In vitro yeast reconstituted translation system reveals function of eIF5A for synthesis of long polypeptide.


:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inserted at the internal position of the protein arrests translation exclusively at low Mg2+ concentrations, and peptidylpolyproline-tRNA intrinsically destabilizes 80S ribosomes. We demonstrate that unmodified eIF5A essentially resolves such ribosome stalling; however, the hypusine modification drastically stimulates ability of eIF5A to rescue polyproline-mediated ribosome stalling and is particularly important for the efficient translation of the N-terminal or long internal polyproline motifs.


J Biochem


Journal of biochemistry


Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N




Has Abstract


2020-05-01 00:00:00














  • Immunological characterization of gamma-glutamyltransferases in human serum.

    abstract::The immunological properties of gamma-glutamyltransferases (gamma-GTs) from human serum, liver and tonsil were studied by using a monospecific antibody to human kidney gamma-GT for the purpose of elucidating their isozymic relationships. gamma-GTs partially purified from liver and tonsil were indistinguishable in this...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsuchida S,Imai F,Sato K

    更新日期:1981-03-01 00:00:00

  • Neutral glycosphingolipids and gangliosides of bovine thyroid.

    abstract::By the application of a ganglioside-mapping technique, the lipid composition of bovine thyroid was analyzed systematically. The contents of cholesterol, lipid-bound phosphorus and lipid-bound sialic acid in bovine thyroid were 5.10, 9.76, and 0.28 mumol/g of dry tissue, respectively, and the molar ratio of cholesterol...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Iwamori M,Sawada K,Hara Y,Nishio M,Fujisawa T,Imura H,Nagai Y

    更新日期:1982-06-01 00:00:00

  • Thermotropic transition in the states of proteins in sarcoplasmic reticulum vesicles.

    abstract::Interaction between protein and phospholipid molecules in sarcoplasmic reticulum (SR) was studied by measurement of the conformational fluctuation of the protein using the kinetics of the hydrogen-deuterium exchange reaction. It was revealed that the state of SR ATPase undergoes a phase transition at about 18 degrees ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kirino Y,Anzai K,Shimizu H,Ohta S,Nakanishi M,Tsuboi M

    更新日期:1977-10-01 00:00:00

  • Photocontrol of calmodulin interaction with target peptides using azobenzene derivative.

    abstract::Calmodulin (CaM), a physiologically important Ca(2+)-binding protein, participates in numerous cellular regulatory processes. It is dumbbell shaped and contains two globular domains connected by a short alpha-helix. Each of the globular domains has two Ca(2+)-binding sites, the EF hands. CaM undergoes a conformational...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shishido H,Yamada MD,Kondo K,Maruta S

    更新日期:2009-10-01 00:00:00

  • Solubilization and characterization of dehydrodolichyl diphosphate synthase from the yeast Saccharomyces carlsbergensis.

    abstract::When the membrane fraction of Saccharomyces carlsbergensis was incubated with radiolabeled isopentenyl diphosphate in the presence of farnesyl diphosphate and Mg2+, phosphorylated and free long-chain polyprenols were formed. The reaction was inhibited by EDTA and heavy metal cations. A series of non-ionic detergents w...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Bukhtiyarov YE,Shabalin YA,Kulaev IS

    更新日期:1993-06-01 00:00:00

  • Triacylglycerol lipases in adrenals and testes of rats.

    abstract::Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column. The enzymes bound to the column were...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Murase T,Yamada N,Akanuma Y,Ohsawa N

    更新日期:1982-09-01 00:00:00

  • Structural aspects of skeletal muscle F-actin as studied by tryptic digestion: evidence for a second nucleotide interacting site.

    abstract::Trypsin was used as a probe of F-actin conformation. F-actin is known to be refractory to proteolysis [Jacobson, G.R. and Rosenbusch, J.P. (1976) Proc. Natl. Acad. Sci. U.S. 73, 2742-2746]. However, here it was found that F-actin could also be digested by trypsin to a 33-kDa fragment (like G-actin) when free MgADP is ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hozumi T

    更新日期:1988-08-01 00:00:00

  • Purification and characterization of an esterase hydrolyzing monoalkyl phthalates from Micrococcus sp. YGJ1.

    abstract::An esterase that specifically hydrolyzes medium-chain (C(3)-C(5)) monoalkyl phthalates was purified from phthalate-grown Micrococcus sp. YGJ1. The enzyme activity was split into two fractions by hydrophobic chromatography on Phenyl Sepharose, and the enzymes were purified to homogeneity from each fraction. The purifie...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Maruyama K,Akita K,Naitou C,Yoshida M,Kitamura T

    更新日期:2005-01-01 00:00:00

  • Human arylhydrocarbon receptor: functional expression and chromosomal assignment to 7p21.

    abstract::We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. Th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ema M,Matsushita N,Sogawa K,Ariyama T,Inazawa J,Nemoto T,Ota M,Oshimura M,Fujii-Kuriyama Y

    更新日期:1994-10-01 00:00:00

  • Immunoblotting analysis of plasma protein processing in the secretory pathway of rat liver: identification of proteolytic conversion sites of complement pro-C3 and prohaptoglobin.

    abstract::Using an immunoblotting technique, we have studied the processing of plasma proteins in subcellular fractions of rat liver including rough and smooth microsomes and the Golgi subfractions. Each subcellular fraction was directly subjected to SDS-polyacrylamide gel electrophoresis and analyzed by immunoblotting with ant...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oda K,Miki K,Hirose S,Takami N,Misumi Y,Ikehara Y

    更新日期:1986-12-01 00:00:00

  • Formation of cadmium-binding peptide allomorphs in fission yeast.

    abstract::It has been reported that two kinds of Cd-binding peptide (Cd-BP1 and Cd-BP2) are induced in fission yeast upon exposure to Cd, and that they consist of the same unit peptide (cadystin), but Cd-BP1 binds 1.5 times more Cd atoms per cadystin than Cd-BP2 (Murasugi, A., Wada, C., & Hayashi, Y. (1981) J. Biochem. 90, 1561...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Murasugi A,Wada Nakagawa C,Hayashi Y

    更新日期:1984-11-01 00:00:00

  • Binding of sulphatide to recombinant haemagglutinin of influenza A virus produced by a baculovirus protein expression system.

    abstract::Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

    更新日期:2010-04-01 00:00:00

  • Immunochemical difference between cathepsin D and cathepsin E-like enzyme from rat spleen.

    abstract::The immunological properties of acid proteinases from rat spleen, two types of cathepsin D and a cathepsin E-like enzyme, were examined. The rabbit antiserum was prepared against the major form of cathepsin D (cathepsin D-I) from rat spleen. The antiserum quantitatively precipitated the enzyme activity from the purifi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamamoto K,Kamata O,Katsuda N,Kato K

    更新日期:1980-02-01 00:00:00

  • DCCD-sensitive, Na+-dependent H+-influx process coupled to membrane potential formation in membrane vesicles of Halobacterium halobium.

    abstract::The effects of N,N'-dicyclohexylcarbodiimide (DCCD) on light-induced H+-transport and transmembrane electric potential (delta phi) formation were studied in the membrane vesicles of Halobacterium halobium R1M1. In accordance with our previous finding of the existence of two DCCD-binding components in vesicle membrane ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Murakami N,Konishi T

    更新日期:1985-10-01 00:00:00

  • Phosphorylation of CaBP1 and CaBP2 by protein kinase CK2.

    abstract::Several proteins in the mammalian endoplasmic reticulum are substrates for protein kinases. Many unidentified phosphoproteins from this compartment are described in the literature, and this prompted us to try to identify at least the more dominant ones. When solubilized bovine and murine microsomes were phosphorylated...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Janson IM,Ek B,Ek P

    更新日期:1997-01-01 00:00:00

  • The localization of 4-O-methylglucuronic acid-containing glycosphingolipid on the cell surface of bivalve spermatozoa by use of an immunological method.

    abstract::Specific and high titer antisera against 4-O-methylglucuronic acid-containing glycosphingolipid (Lipid IV) from spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, were raised in rabbits. The antisera were found to agglutinate spermatozoa of three fresh-water bivalves, H. schlegelii, Anodonta woodiana, and C...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sugita M,Inoue T,Itasaka O,Hori T

    更新日期:1984-03-01 00:00:00

  • A 26K fragment of troponin T from rabbit skeletal muscle.

    abstract::A 26K fragment of troponin T, which was produced by endogenous proteases in rabbit skeletal muscle, was isolated by SE-Sephadex column chromatography. This fragment sensitized both superprecipitation and ATPase of actomyosin to calcium ions, to the same extent as troponin T. There was no difference in affinity for tro...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohtsuki I,Shiraishi F,Suenaga N,Miyata T,Tanokura M

    更新日期:1984-05-01 00:00:00

  • Cloning of the RNA polymerase alpha subunit gene from Thermus thermophilus HB8 and characterization of the protein.

    abstract::The region containing the RNA polymerase alpha subunit (RNAPalpha) gene (rpoA) and the ribosomal protein genes of a thermophilic eubacterial strain, Thermus thermophilus (Tt) HB8, was cloned from a genomic DNA library by Southern hybridization. The gene order in this region is rpl36-rps13-rps11-rps4-rpoA-rpl17, which ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Wada T,Yamazaki T,Kuramitsu S,Kyogoku Y

    更新日期:1999-01-01 00:00:00

  • Enhancement of transfection efficiency by protamine in DDAB lipid vesicle-mediated gene transfer.

    abstract::We have previously developed a simple gene transfection procedure mediated by cationic lipid vesicles for animal cells, in which a commercially available cationic surfactant, dimethyldioctadecyl ammonium bromide (DDAB), was used for making lipid vesicles. In the present study, we examined enhancement of transfection e...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: You J,Kamihira M,Iijima S

    更新日期:1999-06-01 00:00:00

  • Divalent cation binding properties of slow skeletal muscle troponin in comparison with those of cardiac and fast skeletal muscle troponins.

    abstract::1. New methods of preparing troponins from slow skeletal and cardiac muscle of the chicken have been developed. The electrophoretic mobilities of slow skeletal muscle troponin subunits were different from those of the corresponding fast skeletal muscle subunits. 2. A new method for determining the amount of divalent c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kohama K

    更新日期:1979-09-01 00:00:00

  • Conformation of the fowl protamine, galline, and its binding properties to DNA.

    abstract::1. CD spectra showed that the fowl protamine, galline, has an unordered structure rich in reverse turns in neutral solution. Eight reverse turns were predicted to be present in the galline molecule on the basis of its amino acid sequence. Spectrophotometric analyses revealed that galline efficiently bound to DNA in 0....

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakano M,Kasai K,Yoshida K,Tanimoto T,Tamaki Y,Tobita T

    更新日期:1989-01-01 00:00:00

  • A AAA ATPase Cdc48 with a cofactor Ubx2 facilitates ubiquitylation of a mitochondrial fusion-promoting factor Fzo1 for proteasomal degradation.

    abstract::Dynamic functionality of mitochondria is maintained by continual fusion and fission events. A mitochondrial outer membrane protein Fzo1 plays a pivotal role upon mitochondrial fusion by homo-oligomerization to tether fusing mitochondria. Fzo1 is tightly regulated by ubiquitylations and the ubiquitin-responsible AAA pr...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nahar S,Chowdhury A,Ogura T,Esaki M

    更新日期:2020-03-01 00:00:00

  • A cytochrome o-type oxidase of the thermophilic bacterium PS3 grown under air-limited conditions.

    abstract::A cytochrome o-type oxidase from the thermophilic bacterium PS3 grown under air-limited conditions was purified by ion-exchange chromatography in the presence of a non-ionic detergent. The enzyme was composed of three subunits (60, 30, and 16 kDa) and seemed to contain two molecules of heme b as prosthetic groups. It ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sone N,Kutoh E,Sato K

    更新日期:1990-04-01 00:00:00

  • Genetic and molecular properties of human and rat renin-binding proteins with reference to the function of the leucine zipper motif.

    abstract::The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs. The in vitro synthesized RnBPs, with the respective cDNAs, formed heterodimers with porcine renin and homodimers. On comparison o...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Inoue H,Takahashi S,Fukui K,Miyake Y

    更新日期:1991-10-01 00:00:00

  • Structures of the carbohydrate chains of membrane glycoproteins IIb and IIIa of human platelets.

    abstract::Human platelet membrane glycoproteins IIb (GPIIb) and IIIa (GPIIIa), which have been proposed to be subunits of a receptor for fibrinogen, were purified from Triton X-100-solubilized platelet membranes by affinity chromatography on a concanavalin A (Con A)-Sepharose column followed by preparative sodium dodecyl sulfat...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsuji T,Osawa T

    更新日期:1986-11-01 00:00:00

  • Preparation and characterization of a monoclonal antibody against the refolded and functional extracellular domain of rat P2X4 receptor.

    abstract::The P2X4 purinergic receptor is a key molecule in neuropathic pain, particularly in the allodynia after peripheral nerve injury. We therefore sought to establish an anti-P2X4 receptor monoclonal antibody that would be useful for detection and characterization of the P2X4 receptor. We first prepared the refolded extrac...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Igawa T,Higashi S,Abe Y,Ohkuri T,Tanaka H,Morimoto S,Yamashita T,Tsuda M,Inoue K,Ueda T

    更新日期:2013-03-01 00:00:00

  • The degradation rates of type I, II, and III collagens by tadpole collagenase.

    abstract::The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hayashi T,Nakamura T,Hori H,Nagai Y

    更新日期:1980-03-01 00:00:00

  • Sensitive enzyme-immunostaining and densitometric determination on thin-layer chromatography of N-glycolylneuraminic acid-containing glycosphingolipids, Hanganutziu-Deicher antigens.

    abstract::A sensitive enzyme-immunochemical staining method was developed for detection of N-glycolylneuraminic acid (NeuGc)-containing glycosphingolipids (GSLs) on silica gel thin-layer chromatography. The procedure consists of immune reaction among NeuGc-containing GSLs, affinity-purified chicken anti-NeuGc-LacCer and horsera...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Higashi H,Fukui Y,Ueda S,Kato S,Hirabayashi Y,Matsumoto M,Naiki M

    更新日期:1984-05-01 00:00:00

  • Detection of cyclic GMP binding protein and ion channel activity in frog rod outer segments.

    abstract::In order to identify the cGMP-sensitive ion channel protein in frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by means of photoaffinity labeling with [3H]cGMP. We found four cGMP binding proteins with molecular weights (Mws) of 250K, 100K, 92K, and 53K. The 250K protein was an integral-mem...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shinozawa T,Sokabe M,Terada S,Matsusaka H,Yoshizawa T

    更新日期:1987-08-01 00:00:00

  • Simultaneous bindings of ATPase inhibitor and 9K protein to F1F0-ATPase in the presence of 15K protein in yeast mitochondria.

    abstract::Yeast mitochondrial ATP synthase has three regulatory proteins, ATPase inhibitor, 9K protein, and 15K protein. The 9K protein binds directly to purified F1-ATPase, as does the ATPase inhibitor, but the 15K protein does not [Hashimoto, T. et al. (1987) J. Biochem. 102, 685-692]. In the present study, we found that 15K ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hashimoto T,Yoshida Y,Tagawa K

    更新日期:1990-07-01 00:00:00