In vitro yeast reconstituted translation system reveals function of eIF5A for synthesis of long polypeptide.


:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inserted at the internal position of the protein arrests translation exclusively at low Mg2+ concentrations, and peptidylpolyproline-tRNA intrinsically destabilizes 80S ribosomes. We demonstrate that unmodified eIF5A essentially resolves such ribosome stalling; however, the hypusine modification drastically stimulates ability of eIF5A to rescue polyproline-mediated ribosome stalling and is particularly important for the efficient translation of the N-terminal or long internal polyproline motifs.


J Biochem


Journal of biochemistry


Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N




Has Abstract


2020-05-01 00:00:00














  • Fibrinopeptides A and B of Japanese monkey (Macaca fuscata) and patas monkey (Erythrocebus patas): their amino acid sequences, restricted mutations, and a molecular phylogeny for macaques, guenons, and baboons.

    abstract::Amino acid sequences of fibrinopeptides A and B from the macaque, Macaca fuscata (Japanese monkey) and the guenon, Erythrocebus patas (patas monkey) were established. Fibrinopeptides A of the monkeys had a sequence identical with those of baboons: Ala-Asp-Thr-Gly-Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg. Fibrin...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakamura S,Takenaka O,Takahashi K

    更新日期:1985-05-01 00:00:00

  • Sulfation of chondroitin sulfate secreted by baby hamster kidney cells and their polyoma virus-transformed counterparts.

    abstract::Sulfation of glycosaminoglycans (GAGs) secreted by baby hamster kidney (BHK) cells and the polyoma virus-transformants (PY-BHK) was investigated. It has been reported that chondroitin sulfate (CS) of cell membranes from PY-BHK cells is undersulfated compared to that from BHK cells (Cancer Res. 43, 2712-2717, 1983). In...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sugahara K,Fukui S,Yamashina I

    更新日期:1985-10-01 00:00:00

  • Subunit compositions of trypsin inhibitors from tubers of taro, Colocasia antiquorum var. nymphaifolia?

    abstract::Several trypsin inhibitors with different mobilities on polyacrylamide gel electrophoresis occur in the tubers of taro (Colocasia antiquorum), and they each have a dimeric molecular weight of 40,000. Of all the constituent subunits, molecular weight 20,000, of the taro trypsin inhibitor (TTI), three major subunit comp...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ogata F,Makisumi S

    更新日期:1985-02-01 00:00:00

  • Ca2+-induced distance change between points on actin and troponin in skeletal muscle thin filaments estimated by fluorescence energy transfer spectroscopy.

    abstract::Fluorescence resonance energy transfer spectroscopy has been used to study the spatial relationships between probes attached to actin and troponin in the reconstituted skeletal muscle thin filament in the presence and absence of Ca2+ ions. Gln-41 and the nucleotide-binding site of actin were selectively labeled with t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Miki M,Kobayashi T,Kimura H,Hagiwara A,Hai H,Maéda Y

    更新日期:1998-02-01 00:00:00

  • LncRNA MEG3 inhibits the progression of prostate cancer by facilitating H3K27 trimethylation of EN2 through binding to EZH2.

    abstract::This study aims to study the effects of intra-nuclear lncRNA MEG3 on the progression of prostate cancer and the underlying mechanisms. Expressions of relative molecules were detected by Quantitative real time PCR (qRT-PCR) and western blot. Chromatin immunoprecipitation and RNA immunoprecipitation (RIP) assays were us...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Zhou Y,Yang H,Xia W,Cui L,Xu R,Lu H,Xue D,Tian Z,Ding T,Cao Y,Shi Q,He X

    更新日期:2020-03-01 00:00:00

  • Purification and properties of epithelial growth inhibitor (EGI) from human platelets: its separation from type beta transforming growth factor (TGF-beta).

    abstract::We previously reported that sera from various kinds of animals contain a protein(s) capable of inhibiting the growth of the non-malignant epithelial cell line derived from Buffalo rat liver (BRL). In the present study, a similar epithelial cell-specific growth inhibitor (EGI) was purified to homogeneity from an acid-e...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Huang W,Kimura T,Mashima K,Miyazaki K,Masaki H,Yamashita J,Horio T

    更新日期:1986-09-01 00:00:00

  • 2'-Deoxy-2'-azidoadenosine triphosphate and 2'-deoxy-2'-fluoroadenosine triphosphate as substrates and inhibitors for Escherichia coli DNA-dependent RNA polymerase.

    abstract::The effects of 2'-substitutions of ATP on the substrate and inhibitor properties for RNA synthesis were studied in the poly(dAT)-dependent reaction of Escherichia coli RNA polymerase. In the presence of UTP, 2'-deoxy-2'-azidoadenosine 5'-triphosphate (AZTP) was incorporated into an acid-insoluble fraction at one-tenth...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ishihama A,Enami M,Nishijima Y,Fukui T,Ohtsuka E,Ikehara M

    更新日期:1980-03-01 00:00:00

  • Identification of mK1, a true tissue (glandular) kallikrein of mouse submandibular gland: tissue distribution and a comparison of kinin-releasing activity with other submandibular kallikreins.

    abstract::The protein structure, kinin-releasing activity, and tissue distribution of four major proteinases of mouse submandibular gland (mK22, mK9, proteinase F, proteinase P) were studied. When compared with the deduced amino acid sequence of each member of the tissue (glandular) kallikrein gene family, the amino acid sequen...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hosoi K,Tsunasawa S,Kurihara K,Aoyama H,Ueha T,Murai T,Sakiyama F

    更新日期:1994-01-01 00:00:00

  • Biosynthesis of enzyme-bound formylvaline and formylvalylglycine. A possible initiation complex for gramicidin A biosynthesis.

    abstract::A partially purified fraction (Component I) which catalyzed the ATP-PPi exchange reaction dependent only on valine and glycine among the five constituent amino acids of gramicidin A was obtained from crude extracts of Bacillus brevis ATCC 8185, which produces both gramicidin A and tyrocidines. This fraction was found ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Akashi K,Kubota K,Kurahashi K

    更新日期:1977-01-01 00:00:00

  • Multiple forms of ribonuclease H from rat liver cytosol.

    abstract::Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sawai Y,Yanokura M,Tsukada K

    更新日期:1979-09-01 00:00:00

  • Cloning and nucleotide sequences of NADH-putidaredoxin reductase gene (camA) and putidaredoxin gene (camB) involved in cytochrome P-450cam hydroxylase of Pseudomonas putida.

    abstract::Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. Cytochrome P-450cam and related enzymes participate in the early oxidation steps of D-camphor degradation metabolism. We cloned from a HindIII DNA lib...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Koga H,Yamaguchi E,Matsunaga K,Aramaki H,Horiuchi T

    更新日期:1989-11-01 00:00:00

  • Characterization of human serum mannan-binding protein promoter.

    abstract::Serum mannan binding protein (MBP), a mannose/N-acetylglusosamine-specific lectin, is important in innate immunity. In order to elucidate the mechanism underlying the wide intra- and interracial variety in the MBP serum level, we have studied the transcriptional regulation of human MBP. Rapid amplification of cDNA end...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Naito H,Ikeda A,Hasegawa K,Oka S,Uemura K,Kawasaki N,Kawasaki T

    更新日期:1999-12-01 00:00:00

  • Use of SpyTag/SpyCatcher to construct bispecific antibodies that target two epitopes of a single antigen.

    abstract::Bispecific antibody targeting of two different antigens is promising, but when fragment-based antibodies are used, homogeneous production is difficult. To overcome this difficulty, we developed a method using the SpyTag/SpyCatcher system in which a covalent bond is formed between the two polypeptides. Using this metho...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yumura K,Akiba H,Nagatoishi S,Kusano-Arai O,Iwanari H,Hamakubo T,Tsumoto K

    更新日期:2017-09-01 00:00:00

  • Thiobacillus novellus cytochrome c oxidase contains one heme alpha molecule and one copper atom per catalytic unit.

    abstract::The minimal structural unit of cytochrome c oxidase purified from Thiobacillus novellus was composed of one molecule each of two subunits with molecular masses of 32 and 23 kDa, respectively, and the unit had one molecule of heme a and one atom of copper. In the presence of n-octyl-beta-D-thioglucoside, the oxidase ex...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shoji K,Yamazaki T,Nagano T,Fukumori Y,Yamanaka T

    更新日期:1992-01-01 00:00:00

  • Purification and properties of xanthine dehydrogenase from Streptomyces cyanogenus.

    abstract::Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 45...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohe T,Watanabe Y

    更新日期:1979-07-01 00:00:00

  • The modification of sulfhydryl groups of glutamine synthetase from Bacillus stearothermophilus with 5, 5'-dithiobis(2-nitrobenzoic acid).

    abstract::The SH groups of glutamine synthetase [EC] from Bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of SH groups in the molecule as well as the effect of the modification on the enzyme activity. Three SH groups per subunit were detected after co...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hachimori A,Takeda A,Nagaoka T,Suzuki H,Nosoh Y,Samejima T

    更新日期:1975-12-01 00:00:00

  • Binding of actin filaments to connectin.

    abstract::The binding of actin filaments to connectin, a muscle elastic protein, was investigated by means of turbidity and sedimentation measurements and electron microscopy. In the presence of less than 0.12 M KCl at pH 7.0, actin filaments bound to connectin. Long actin filaments formed bundles. Short actin filaments also ag...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Maruyama K,Hu DH,Suzuki T,Kimura S

    更新日期:1987-06-01 00:00:00

  • Aspartate aminotransferase from an alkalophilic Bacillus contains an additional 20-amino acid extension at its functionally important N-terminus.

    abstract::Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

    更新日期:1996-08-01 00:00:00

  • Properties of amino acid composition of the tryptic fragments of the heavy chain of myosin subfragment-1.

    abstract::Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry 20, 2945-2950) that these fragments can be separated by gel filtration i...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hozumi T

    更新日期:1981-09-01 00:00:00

  • Purification and characterization of methioninase from Pseudomonas putida.

    abstract::Methioninase of Pseudomonas putida was purified to homogeneity, as judged by polyacrylamide gel electrophoresis, with a specific activity 270-fold higher than that of the crude extract. 1. The purified enzyme had an S20,w of 8.37, a molecular weight of 160,000, and an isoelectric point of 5.6. 2. A break in the Arrhen...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ito S,Nakamura T,Eguchi Y

    更新日期:1976-06-01 00:00:00

  • Structure and function of 5S ribosomal ribonucleic acid from Torulopsis utilis. III. Detection of single-stranded regions by digestion with nuclease S1.

    abstract::Identification of single-stranded regions in Torulopsis utilis 5S RNA was attempted by the use of Nuclease S1, a single-strand specific endonuclease. When T. utilis 5S RNA was subjected to prolonged incubation with Nuclease S1, about 50% of the substrate 5S RNA remained as large oligonucleotide "cores." Such Nuclease...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nishikawa K,Takemura S

    更新日期:1977-04-01 00:00:00

  • Species-barrier phenomenon in prion transmissibility from a viewpoint of protein science.

    abstract::Transmissible spongiform encephalopathies (TSEs), or prion diseases, are fatal infectious neurodegenerative disorders. Their causative agents are prions, which are composed of disease-associated forms of prion protein (PrP(Sc)). Naturally occurring cases of TSEs are found in several mammalian species including humans,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Hagiwara K,Hara H,Hanada K

    更新日期:2013-02-01 00:00:00

  • Characterization of recombinant YakC of Schizosaccharomyces pombe showing YakC defines a new family of aldo-keto reductases.

    abstract::The yakC gene in Schizosaccharomyces pombe, which encodes yakC protein (YakC), a potential member of an aldo-keto reductase (AKR) family, was cloned and expressed in Escherichia coli cells. The recombinant YakC purified to homogeneity catalyzed the reduction of 2-nitrobenzaldehyde (k(cat), 44.1 s(-1), K(m), 0.185 +/- ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morita T,Huruta T,Ashiuchi M,Yagi T

    更新日期:2002-10-01 00:00:00

  • Molecular cloning and nucleotide sequence of a cDNA encoding Euglena gracilis cytochrome c1.

    abstract::The amino acid sequence of the mature protein of Euglena gracilis cytochrome c1 was determined by sequencing of its cDNA. A cDNA expression library was constructed from Euglena poly(A)+ RNA in phage lambda gt11 and screened with an antiserum raised against cytochrome c1 polypeptide isolated from purified E. gracilis c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mukai K,Wakabayashi S,Matsubara H

    更新日期:1989-09-01 00:00:00

  • Interaction of studies of a 100,000 dalton chymotryptic fragment of rabbit skeletal M-line protein with the S2 subfragment of myosin.

    abstract::Controlled chymotryptic digestion of the 165,000 dalton component of the M-line of rabbit skeletal muscle, followed by Biogel P-150 chromatography of the digest, has led to the isolation of a homogeneous 100,000 dalton species. This fragment was found, by both sedimentation equilibrium and gel filtration chromatograph...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mani RS,Kay CM

    更新日期:1979-12-01 00:00:00

  • Antigenic structure of adenylate kinase from porcine skeletal muscle. I. Three immunochemically active peptides obtained by cleavage with cyanogen bromide.

    abstract::Analysis of the quantitative precipitin reaction of adenylate kinase from porcine skeletal muscle with goat anti-adenylate kinase antiserum indicated that there are at least four antigenic determinants on the enzyme molecule. Porcine adenylate kinase was cleaved with cyanogen bromide, and four peptides were fractionat...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Sato N,Koyama Y,Shiokawa H

    更新日期:1982-05-01 00:00:00

  • A new Tetrahymena actin-binding protein is localized in the division furrow.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Watanabe A,Kurasawa Y,Watanabe Y,Numata O

    更新日期:1998-04-01 00:00:00

  • Amino acid sequences of Nostoc strain MAC ferredoxins I and II.

    abstract::The amino acid sequences of ferredoxins I and II from a blue-green alga, Nostoc strain MAC were determined. This alga is able to grow autotrophically in the light or heterotrophically in the dark. Analyses of tryptic peptides of Cm-proteins by conventional methods including solid-phase Edman degradation gave the compl...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hase T,Matsubara H,Hutber GN,Rogers LJ

    更新日期:1982-11-01 00:00:00

  • Ca2+- and Sr2+-sensitivity of the ATPase activity of rabbit skeletal myofibrils: effect of the complete substitution of troponin C with cardiac troponin C, calmodulin, and parvalbumins.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morimoto S,Ohtsuki I

    更新日期:1987-02-01 00:00:00

  • Effects of polyamines on the activities of Escherichia coli ribonuclease I and II.

    abstract::The effects of polyamines on the breakdown of synthetic polynucleotides [poly(A), poly(C), and poly(U)] by E. coli ribonuclease I [ribonucleate 3'-oligonucleotidohydrolase, EC] and ribonuclease II [EC] have been studied. The degradation of poly(C) by RNase II was stimulated by spermine and spermidine,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kumagai H,Igarashi K,Yoshikawa M,Hirose S

    更新日期:1977-02-01 00:00:00