Structure, expression, and evolution of guinea pig serum amyloid P component and C-reactive protein.

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00

abstract：:We have cloned a DNA from a human pancreatic cDNA library using a cloned rat pancreatic elastase 1 cDNA as a probe, and determined its nucleotide sequence. This cDNA contains a coding region of 810 nucleotides which encodes a 270-amino-acid protein. The deduced amino acid sequence shows less than 60% homologies with r...

journal_title：Journal of biochemistry

authors： Shirasu Y,Takemura K,Yoshida H,Sato Y,Iijima H,Shimada Y,Mikayama T,Ozawa T,Ikeda N,Ishida A

更新日期：1988-08-01 00:00:00

abstract：:The gene encoding subtilisin Amylosacchariticus from Bacillus subtilis var. amylosacchariticus was isolated and the entire nucleotide sequence of the coding sequence was determined. The deduced amino acid sequence revealed an N-terminal signal peptide and pro-peptide of 106 residues followed by the mature protein comp...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Oyama H,Honda T,Tone H,Takeshita T,Kamiyama T,Tsuru D

更新日期：1988-06-01 00:00:00

abstract：:The differential assay conditions for protein phosphatases PP1, PP2A, and PP2C were extensively studied by using crude extracts from mouse lymphoid tissues as enzyme sources. Under these conditions, the protein phosphatase activities were measured in MRL/MpJ-lpr/lpr mice (MRL/lpr mice), autoimmune-prone mice, and MRL/...

journal_title：Journal of biochemistry

authors： Matsuzawa S,Tamura T,Mizuno Y,Kobayashi S,Okuyama H,Tsukitani Y,Uemura D,Kikuchi K

更新日期：1992-04-01 00:00:00

abstract：:Comparative studies have been made on the effects of diazoacetyl-DL-norleucine methyl ester (DAN), 1,2-epoxy-3-(p-nitrophenoxy)propane (EPNP) and pepstatin on acid proteases, including those from Acrocylindrium sp., Aspergillus niger, Aspergillus saitoi, Mucor pusillus, Paecilomyces varioti, Rhizopus chinensis, and Tr...

journal_title：Journal of biochemistry

authors： Takahashi K,Chang WJ

更新日期：1976-09-01 00:00:00

abstract：:The 1H-NMR spectra of eight unsaturated disaccharides obtained by bacterial eliminase digestion of chondroitin sulfate and of heparan sulfate/heparin were recorded in order to construct an NMR data base of sulfated oligosaccharides and to investigate the effects of sulfation on the proton chemical shifts. These shifts...

journal_title：Journal of biochemistry

authors： Yamada S,Yoshida K,Sugiura M,Sugahara K

更新日期：1992-10-01 00:00:00

abstract：:In vitro activation of human reovirus transcriptase by alpha-chymotrypsin digestion of viral outer shell proteins was completely dependent on the ionic size of the monovalent cation in the medium. Cations with nonhydrated ionic radii larger than 1.3 A showed full potency of activation of chymotrypsin digestion, and pr...

journal_title：Journal of biochemistry

authors： Nagai J,Tanaka M,Nakashima K

更新日期：1984-03-01 00:00:00

abstract：:We previously showed that the 9 S estrogen receptor (ER) could be reconstituted from purified ER and purified heat shock protein 90 (hsp 90). So, we investigated the role of hsp 90 in the binding of purified ER to an estrogen responsive element (ERE) by using the reconstitution system. ER purified from calf uterus sho...

journal_title：Journal of biochemistry

authors： Inano K,Curtis SW,Korach KS,Omata S,Horigome T

更新日期：1994-10-01 00:00:00

abstract：:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of...

journal_title：Journal of biochemistry

authors： Jing Y,Wang M,Tang W,Qi T,Gu C,Hao S,Zeng X

更新日期：2007-05-01 00:00:00

abstract：:beta-D-Galactosidase has been purified to apparent homogeneity from rabbit spleen. The purification steps involved ammonium sulphate precipitation, DEAE-cellulose, concanavalin A-Sepharose, Sephadex G-200, and Sepharose 4B-(epsilon-aminocaproyl)-2-deoxy-beta-D-glucosylamine affinity chromatographies. In the DEAE-cellu...

journal_title：Journal of biochemistry

authors： Rodríguez-Berrocal FJ,Páez de la Cadena M,Cabezas JA,Pérez-González N

更新日期：1988-07-01 00:00:00

abstract：:Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

journal_title：Journal of biochemistry

authors： Kiyonari S,Egashira Y,Ishino S,Ishino Y

更新日期：2014-05-01 00:00:00

abstract：:The effect of concentration of rabbit reticulocyte rRNAs on the translation of rabbit globin mRNAs was studied by using a rabbit reticulocyte lysate system. Globin synthesis was studied using L-[U-14C]leucine. Both the 18S and 28S rRNA inhibited globin synthesis. The 18S rRNA inhibited the synthesis of alpha-globin ch...

journal_title：Journal of biochemistry

authors： Suzuki H

更新日期：1978-04-01 00:00:00

abstract：:NADP+-specific isocitrate dehydrogenase was found in several tissues of the pupa of the silkworm, Bombyx mori. This enzyme was highly purified from the whole bodies of pupae. This is the first isolation of the enzyme from insect materials. The purified enzyme gave a single protein band on polyacrylamide gel electropho...

journal_title：Journal of biochemistry

authors： Miake F,Torikata T,Koga K,Hayashi K

更新日期：1977-08-01 00:00:00

abstract：:An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance sp...

journal_title：Journal of biochemistry

authors： Waki H,Murata A,Kon K,Maruyama K,Kimura S,Ogura H,Ando S

更新日期：1993-04-01 00:00:00

abstract：:A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and ...

journal_title：Journal of biochemistry

authors： Kinoshita M,Okazaki M,Kato H,Teramoto T,Matsushima T,Naito C,Oka H,Hara I

更新日期：1983-08-01 00:00:00

abstract：:Light harvesting (LH) bacteriochlorophyll (Bchl) protein complexes were isolated from chromatophores of Chromatium vinosum, by the combination of detergent solubilization, sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and hydroxyapatite chromatography. Addition of the absorption spectra of thes...

journal_title：Journal of biochemistry

authors： Hayashi H,Morita S

更新日期：1980-11-01 00:00:00

abstract：:A 5'-N-ethylcarboxamido[3H]adenosine ([3H]NECA) binding site of mouse mastocytoma P815 cell membranes has been purified approximately 100-fold by affinity chromatography. This adenosine binding site, which has a similar specificity to that of the A2 adenosine receptor, was absorbed on NECA-linked Sepharose 6B and elut...

journal_title：Journal of biochemistry

authors： Nakata H

更新日期：1989-05-01 00:00:00

abstract：:Recently, we revealed that the cloverleaf structure of some eukaryotic tRNAs is not always stable in vitro, and the denatured structures of these tRNAs are sometimes detected in bacterial RNase P reactions. We have designated the unusual internal cleavage reaction of these tRNAs as hyperprocessing. We have developed t...

journal_title：Journal of biochemistry

authors： Tanaka T,Kondo Y,Hori Y,Kikuchi Y

更新日期：2002-06-01 00:00:00

abstract：:To examine whether serine proteases of rat liver chromatin are also involved in the degradation of newly synthesized and unbound ribosomal proteins and histones, like the nuclear thiol protease which we reported previously (Tsurugi, K. & Ogata, K. (1979) Eur. J. Biochem. 101, 205-213), in vivo experiments were carried...

journal_title：Journal of biochemistry

authors： Tsurugi K,Oyanagi M,Ogata K

更新日期：1983-05-01 00:00:00

abstract：:We examined the substrate specificity of two mutated geranylgeranyl diphosphate synthases, I-9 and I-11, with respect to several artificial substrates. These mutated enzymes have replacements in the amino acid sequences from positions 170 to 173, which are thought to be a part of the putative substrate binding region....

journal_title：Journal of biochemistry

authors： Ohnuma S,Hemmi H,Koyama T,Ogura K,Nishino T

更新日期：1998-06-01 00:00:00

abstract：:Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

journal_title：Journal of biochemistry

authors： Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

更新日期：2001-06-01 00:00:00

abstract：:The cytoplasmic peptide elongation factor, EF-1 alpha, is anchored at the endoplasmic reticulum membrane by phosphatidylinositol via ethanolamine bridging presumably to Asp306 of the protein. ...

journal_title：Journal of biochemistry

authors： Hayashi Y,Urade R,Utsumi S,Kito M

更新日期：1989-10-01 00:00:00

abstract：:An esterase that specifically hydrolyzes medium-chain (C(3)-C(5)) monoalkyl phthalates was purified from phthalate-grown Micrococcus sp. YGJ1. The enzyme activity was split into two fractions by hydrophobic chromatography on Phenyl Sepharose, and the enzymes were purified to homogeneity from each fraction. The purifie...

journal_title：Journal of biochemistry

authors： Maruyama K,Akita K,Naitou C,Yoshida M,Kitamura T

更新日期：2005-01-01 00:00:00

abstract：:Streptomyces caespitosus neutral protease (ScNP) is one of the smallest metalloproteinase with a molecular mass of 14 kDa. Effects of solvent composition on ScNP activity were examined using a peptide substrate. The k(cat)/K(m) values of ScNP exhibited bell-shaped pH-dependence with the optimal pH of 6.4-7.0 and the p...

journal_title：Journal of biochemistry

authors： Inouye K,Shimada T,Yasukawa K

更新日期：2007-09-01 00:00:00

abstract：:The Genome of Bacillus subtilis 168 was used for cloning and engineering of large-sized DNAs. A mouse genomic DNA of approximately 120 kb was cloned into a locus of the B. subtilis genome by ordered assembly of 20- to 50-kb mouse DNA segments. Cloned mouse DNA, maintained stably, was engineered through B. subtilis tra...

journal_title：Journal of biochemistry

authors： Itaya M,Nagata T,Shiroishi T,Fujita K,Tsuge K

更新日期：2000-11-01 00:00:00

abstract：:We previously reported that caldesmon (CaD), together with tropomyosin (TM), effectively protects actin filaments from gelsolin, an actin-severing protein. To elucidate the structure/function relationship of CaD, we dissected the functional domain of CaD required for the protection. The basic C-terminal half of rat no...

journal_title：Journal of biochemistry

authors： Takiguchi K,Matsumura F

更新日期：2005-12-01 00:00:00

abstract：:The fatty acid composition of cardiolipin from the Harderian gland of guinea pig was examined by gas chromatography and gas chromatography-mass spectrometry. At least 33 kinds of fatty acids were detected. Oleic acid was the most prominent component, accounting for 18.2 mol% of the total fatty acids. About 70.2 mol% o...

journal_title：Journal of biochemistry

authors： Seyama Y,Ohashi K,Yasugi E,Otsuka H

更新日期：1984-12-01 00:00:00

abstract：:The P1' Ser(50) at the second reactive site of soybean protease inhibitor C-II was replaced with arginine to confirm the contribution of this residue to the inhibition. The Arg derivative had less trypsin inhibitory activity (Ki = 1 X 10(-7) M) than the Ser derivative (Ki = 2 X 10(-8) M), in contrast to the results ob...

journal_title：Journal of biochemistry

authors： Kurokawa T,Hara S,Teshima T,Ikenaka T

更新日期：1987-09-01 00:00:00

abstract：:Using a new cannulation method, which completely prevented interruption of circulation to brain tissue during surgical operations, we could prepare a functionally intact hemoglobin-free isolated perfused rat head in situ. The frequency distribution (8-25 Hz) and amplitude of a spontaneous electroencephalogram, EEG (50...

journal_title：Journal of biochemistry

authors： Inagaki M,Tamura M

更新日期：1993-06-01 00:00:00

abstract：:In porcine aortic smooth muscle cells, neuropeptide Y (NPY) stimulates mobilization of CA(2+) from intracellular store sites via Y1 receptors. However, it has been debated whether or not Ca(2+) mobilization by Y1 receptors depends on the generation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] following activation of...

journal_title：Journal of biochemistry

authors： Shigeri Y,Nakajima S,Fujimoto M

更新日期：1995-09-01 00:00:00