Conformation and template activity of human reovirus genome RNA.

abstract：:The fructose 2,6-bisphosphate concentrations in unwashed, washed, and leukocyte-free erythrocytes were compared. The concentration in washed red cells was 31 +/- 15 pmol per ml of cells (mean +/- S.D., n = 6). The concentration in unwashed erythrocytes was at least twofold higher, but the value in washed red cells was...

journal_title：Journal of biochemistry

authors： Moriyasu M,Yamamoto M,Hamasaki N

更新日期：1988-06-01 00:00:00

abstract：:The substrate specificity of microsomal nonspecific carboxyesterase [EC 3.1.1.1] from rabbit and rat livers was studied in vitro by using seven (A)-type and six (B)-type 12,13-epoxytrichothecene mycotoxins. The C-4 acetyl residues of diacetoxyscirpenol, T-2 toxin, monoacetylnivalenol (fusarenon-X), and diacetylnivalen...

journal_title：Journal of biochemistry

authors： Ohta M,Matsumoto H,Ishii K,Ueno Y

更新日期：1978-09-01 00:00:00

abstract：:The chromatin remodeling complex, SWI/SNF, is known to regulate the transcription of several genes by altering the chromatin structure in an ATP-dependent manner. SWI/SNF exclusively contains BRG1 or BRM as an ATPase subunit. In the present study, we studied the role of SWI/SNF containing BRM or BRG1 in the expression...

journal_title：Journal of biochemistry

authors： Inayoshi Y,Kaneoka H,Machida Y,Terajima M,Dohda T,Miyake K,Iijima S

更新日期：2005-10-01 00:00:00

abstract：:The stalk protein L12 is the only multiple component in 50S ribosomal subunit. In Escherichia coli, two L12 dimers bind to the C-terminal domain of L10 to form a pentameric complex, L10[(L12)(2)](2), while the recent X-ray crystallographic study and tandem MS analyses revealed the presence of a heptameric complex, L10...

journal_title：Journal of biochemistry

authors： Nomura T,Nakatsuchi M,Sugita D,Nomura M,Kaminishi T,Takemoto C,Shirouzu M,Miyoshi T,Yokoyama S,Hachimori A,Uchiumi T

更新日期：2008-11-01 00:00:00

abstract：:beta-Glycosidase from the extreme thermophilic archaeon Sulfolobus solfataricus is a tetrameric protein with a molecular mass of 240 kDa, stable in the presence of detergents, and with a maximal activity at temperatures above 95 degrees C. Understanding the structure-activity relationships of the enzyme under differen...

journal_title：Journal of biochemistry

authors： D'Auria S,Nucci R,Rossi M,Bertoli E,Tanfani F,Gryczynski I,Malak H,Lakowicz JR

更新日期：1999-09-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:A highly sensitive method was developed for quantitative analysis of phospholipid molecular species. Diradylglycerols prepared from phospholipids with phospholipase C were converted to the anthroyl-diradylglycerol derivatives, which could be separated into molecular species and sensitively quantified by reverse-phase ...

journal_title：Journal of biochemistry

authors： Takamura H,Kito M

更新日期：1991-03-01 00:00:00

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00

abstract：:CXCL14 is a member of the CXC chemokine family. CXCL14 possesses chemoattractive activity for activated macrophages, immature dendritic cells and natural killer cells. CXCL14-deficient mice do not exhibit clear immune system abnormalities, suggesting that the function of CXCL14 can be compensated for by other chemokin...

journal_title：Journal of biochemistry

authors： Hara T,Tanegashima K

更新日期：2012-05-01 00:00:00

abstract：:Human promyelocytic leukemia cells (HL-60) were treated with several differentiation inducers, then the changes in the activity of cytosolic protein kinase C (PKC) isoforms were examined by hydroxylapatite chromatography and the species of the isoforms were determined immunologically. In three undifferentiated HL-60 c...

journal_title：Journal of biochemistry

authors： Tanaka Y,Yoshihara K,Tsuyuki M,Itaya-Hironaka A,Inada Y,Kamiya T

更新日期：1992-02-01 00:00:00

abstract：:Ion-exchange column-purified I-protein was labeled by fluorescein isothiocyanate (FITC) at an equimolar ratio. When FITC-labeled I-protein was reacted with glycerinated myofibrils of chicken breast muscle in a phosphate-buffered saline, fluorescence was observed at the A-band and/or the Z-line of the sarcomere. Howeve...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1988-02-01 00:00:00

abstract：:Epithelial-mesenchymal transition (EMT) is a morphogenetic process of cells that adopt an epithelial organization in their developmental ontogeny or homeostatic maintenance. Abnormalities in EMT regulation result in many malignant tumours in the human body. Tumours associated with the haematopoietic system, however, a...

journal_title：Journal of biochemistry

authors： Hamidi S,Sheng G

更新日期：2018-10-01 00:00:00

abstract：:The interactions of porcine alpha2-macroglobulin (alpha2M) with native proteinases, their zymogens and the chemically-modified enzymes were compared. The alpha2M did not bind to chymotrypsinogen, or to most of the chemically modified derivatives of alpha-chymotrypsin, trypsinogen, DIP- and PMS-trypsins, but it could i...

journal_title：Journal of biochemistry

authors： Tsuru D,Kado K,Fujiwara K,Tmimatsu M,Ogita K

更新日期：1978-05-01 00:00:00

abstract：:The activity and stability of mushroom tyrosinase were studied in ionic liquid (IL)-containing aqueous systems. The effect of three ILs ([BMIm][PF(6)], [BMIm][BF(4)]), and [BMIm][MeSO(4)], where [BMIm] = 1-butyl-3-methylimidazolium) and their inorganic salts (KMeSO(4), KPF(6), and NaBF(4)) on the enzyme performance wa...

journal_title：Journal of biochemistry

authors： Yang Z,Yue YJ,Huang WC,Zhuang XM,Chen ZT,Xing M

更新日期：2009-03-01 00:00:00

abstract：:In the embryonic smooth muscle of chicken gizzards we found 4 high-Mr-type and 5 low-Mr-type tropomyosin isoforms in addition to alpha- and beta-isoforms reported already. The criteria by which they were identified as tropomyosin isoforms were as follows: 1) anomalous reduction of electrophoretic mobility in the prese...

journal_title：Journal of biochemistry

authors： Hosoya M,Miyazaki J,Hirabayashi T

更新日期：1989-05-01 00:00:00

abstract：:PKNalpha is a fatty acid- and Rho-activated serine/threonine protein kinase having a catalytic domain homologous to members of the protein kinase C family. Recently it was reported that PKNalpha is involved in the p38 mitogen-activated protein kinase (MAPK) signaling pathway. To date, however, how PKNalpha regulates t...

journal_title：Journal of biochemistry

authors： Takahashi M,Gotoh Y,Isagawa T,Nishimura T,Goyama E,Kim HS,Mukai H,Ono Y

更新日期：2003-02-01 00:00:00

abstract：:The stress-related protein Stm1 interacts with ribosomes, and is implicated in repressing translation. Stm1 was previously studied both in vivo and in vitro by cell-free translation systems using crude yeast lysates, but its precise functional mechanism remains obscure. Using an in vitro reconstituted translation syst...

journal_title：Journal of biochemistry

authors： Hayashi H,Nagai R,Abe T,Wada M,Ito K,Takeuchi-Tomita N

更新日期：2018-03-01 00:00:00

abstract：:The Escherichia coli strain carrying pTP 6-10 which was constructed in our previous work (Iwakura, M., et al. (1983) J. Biochem. 93, 927-930) produces more than 400-fold dihydrofolate reductase as compared with the strain without the plasmid. Dihydrofolate reductase was highly purified from the cell-free extract of th...

journal_title：Journal of biochemistry

authors： Iwakura M,Shimura Y,Sakai T,Tsuda K

更新日期：1983-09-01 00:00:00

abstract：:In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papillomavirus vector DNA. After co-transfection of mouse C127 cells with...

journal_title：Journal of biochemistry

authors： Nakamura Y,Oda K,Nakada S

更新日期：1991-09-01 00:00:00

abstract：:We have unexpectedly found that Streptomyces subtilisin inhibitor (SSI) and some other similar serine protease inhibitors produced by Streptomycetes strongly inhibit Streptomyces griseus metallo-endopeptidase II (SGMP II) [Kajiwara, K. et al. (1991) J. Biochem. 110, 350-354]. In order to elucidate the mode of their un...

journal_title：Journal of biochemistry

authors： Kumazaki T,Kajiwara K,Kojima S,Miura K,Ishii S

更新日期：1993-10-01 00:00:00

abstract：:The effect of polyamines on F1-ATPase catalyzed reactions has been studied through the use of submitochondrial particles and F1-ATPase. ATP degradation catalyzed by submitochondrial particles and F1-ATPase was inhibited by spermine and spermidine. Spermine's inhibition was much greater than spermidine's effect. In con...

journal_title：Journal of biochemistry

authors： Igarashi K,Kashiwagi K,Kobayashi H,Ohnishi R,Kakegawa T,Nagasu A,Hirose S

更新日期：1989-08-01 00:00:00

abstract：:A variant of human tear lipocalin was expressed in Escherichia coli, and the bound fatty acids were analysed by gas chromatography, mass spectroscopy and nuclear magnetic resonance spectroscopy. Five major fatty acids were identified as hexadecanoic acid (palmitic acid, PA), cis-9-hexadecenoic acid (palmitoleic acid),...

journal_title：Journal of biochemistry

authors： Tsukamoto S,Fujiwara K,Ikeguchi M

更新日期：2009-09-01 00:00:00

abstract：:Monoclonal antibody 5E5 recognized an intranuclear antigen of neurons in the rat. We isolated 5E5cDNA and determined its nucleotide and deduced amino acid sequences. The 5E5cDNA had an open reading frame of 825 amino acids and its amino acid sequence showed no significant homology to any protein or to any DNA binding ...

journal_title：Journal of biochemistry

authors： Suzuki E,Kojima N,Yoshimura K,Uyemura K,Obata K,Akagawa K

更新日期：1995-07-01 00:00:00

abstract：:GM2 containing NeuGc was found to be a major ganglioside in the liver of most inbred strains of mouse we have examined so far. They were DBA/2, C3H/He, C57BL/6, BALB/c, and CBA mice. However, WHT/Ht mouse does not contain a detectable amount of GM2 (NeuGc) but has GM3 containing NeuGc as the major ganglioside in the l...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Otsuka H,Sudo K,Suzuki K,Suzuki A,Yamakawa T

更新日期：1983-03-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

journal_title：Journal of biochemistry

authors： Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

更新日期：1996-08-01 00:00:00

abstract：:The region containing the RNA polymerase alpha subunit (RNAPalpha) gene (rpoA) and the ribosomal protein genes of a thermophilic eubacterial strain, Thermus thermophilus (Tt) HB8, was cloned from a genomic DNA library by Southern hybridization. The gene order in this region is rpl36-rps13-rps11-rps4-rpoA-rpl17, which ...

journal_title：Journal of biochemistry

authors： Wada T,Yamazaki T,Kuramitsu S,Kyogoku Y

更新日期：1999-01-01 00:00:00

abstract：:The interaction between lipopolysaccharide (LPS) and an LPS-sensitive serine protease zymogen, factor C, purified from horseshoe crab (Tachypleus tridentatus) hemocytes, was investigated to elucidate the LPS-mediated activation of factor C. The rate of activation of the zymogen factor C was highly dependent on the con...

journal_title：Journal of biochemistry

authors： Nakamura T,Tokunaga F,Morita T,Iwanaga S

更新日期：1988-02-01 00:00:00

abstract：:Tetrahydrobiopterin (BH(4)) acts as a cofactor of the aromatic amino-acid hydroxylases, and its deficiency may result in hyperphenylalaninemia (HPA) and decreased production of the neurotransmitters. BH(4) is synthesized by sepiapterin reductase (SPR) from 6-pyruvoyl-tetrahydropterin (PPH(4)). A patient with SPR defic...

journal_title：Journal of biochemistry

authors： Hirakawa H,Sawada H,Yamahama Y,Takikawa S,Shintaku H,Hara A,Mase K,Kondo T,Iino T

更新日期：2009-07-01 00:00:00

abstract：:The most reactive single thiol group of rabbit skeletal muscle phosphofructokinase per protomer was modified with the following thiol reagents: iodoacetamide, iodoacetate, 2-hydroxyethyl disulfide, 3,3'-dithiodipropionate, and glutathione disulfide. As a result of the modification, there was increase in not only the a...

journal_title：Journal of biochemistry

authors： Nakajima Y,Nakamura K

更新日期：1991-02-01 00:00:00

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00