FITC-labeled I-protein specifically binds to A-bands and/or Z-lines of glycerinated myofibrils of chicken breast muscle.

abstract：:The mechanism of binding between thermolysin with its specific inhibitor, talopeptin (MKI), was studied kinetically with the stopped-flow method by monitoring the enhancement of tryptophan fluorescence caused by the complex formation. Only one relaxation obeying first-order kinetics was observed. The dependence of the...

journal_title：Journal of biochemistry

authors： Kitagishi K,Hiromi K

更新日期：1983-01-01 00:00:00

abstract：:A novel fucose-containing neutral glycosphingolipid (GL-5) was purified from the eggs of the sea urchin, Hemicentrotus pulcherrimus. The chemical structure was determined to be Fuc alpha 1-3GalNAc beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-4Glc beta 1-1Cer by methylation analysis, partial acid hydrolysis, fast atom bombardm...

journal_title：Journal of biochemistry

authors： Kubo H,Jiang GJ,Irie A,Suzuki M,Inagaki F,Hoshi M

更新日期：1992-08-01 00:00:00

abstract：:The alkalophile NADH dehydrogenase (NADH: 2,6-dichlorophenolindophenol oxidoreductase) [EC 1.6.99.3] consists of two identical subunits of 65 kDa, and each subunit contains the catalytic and liposome-binding regions. On treatment with trypsin, the polypeptide exhibiting the liposome-binding property in one of the subu...

journal_title：Journal of biochemistry

authors： Xu XM,Kanaya S,Koyama N,Sekiguchi T,Nosoh Y,Ohashi S,Tsuda K

更新日期：1989-04-01 00:00:00

abstract：:The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...

journal_title：Journal of biochemistry

authors： Hayashi T,Nakamura T,Hori H,Nagai Y

更新日期：1980-03-01 00:00:00

abstract：:HSP47 is a stress protein (heat shock protein) which resides in the endoplasmic reticulum, and is postulated to function as a collagen-specific molecular chaperone. To elucidate the role of HSP47 in procollagen biosynthesis, we have established human embryonic kidney 293 cell lines, which were stably transfected with ...

journal_title：Journal of biochemistry

authors： Hosokawa N,Hohenadl C,Satoh M,Kühn K,Nagata K

更新日期：1998-09-01 00:00:00

abstract：:Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68.2% sequence identity with the yeast homologue at the amino acid and nucleotide level, respectively, while it differs by on...

journal_title：Journal of biochemistry

authors： Takahashi A,Muramatsu H,Takagi S,Fujisawa H,Miyake Y,Muramatsu T

更新日期：2001-04-01 00:00:00

abstract：:An acidic protein of human saliva, which we named SAP-1 previously, is now shown to be an inhibitor of several cysteine proteinases. The protein inhibited papain and ficin strongly, and stem bromelain and bovine cathepsin C partially. However, it did not inhibit either porcine cathepsin B or clostripain. The mode of t...

journal_title：Journal of biochemistry

authors： Isemura S,Saitoh E,Ito S,Isemura M,Sanada K

更新日期：1984-10-01 00:00:00

abstract：:Ca2+-Activated neutral protease (CANP), that consists of 80K and 30K subunits, is converted to a low-Ca2+-requiring form by autolysis in the presence of Ca2+. Phosphatidylinositol greatly reduces the Ca2+-requirement for the autolysis of native CANP. However, this effect was not observed for CANP with a trimmed 30K su...

journal_title：Journal of biochemistry

authors： Imajoh S,Kawasaki H,Suzuki K

更新日期：1986-04-01 00:00:00

abstract：:Tryptophan residues in ribonuclease from a Rhizopus sp. (RNase Rh) were modified by NBS, H2O2-dioxane, o-nitrophenylsulfenyl chloride (NPS-Cl) and the relation between the extent of modification and enzymatic activity was studied in each case. By extrapolation of the modified tryptophan residue-enzymatic activity curv...

journal_title：Journal of biochemistry

authors： Sanda A,Irie M

更新日期：1980-04-01 00:00:00

abstract：:Genomic clones of UDP-glucuronosyltransferase family 1 (UGT1) were isolated from wild-type Wistar rats. The UGT1 locus spans > 120 kb and forms a gene complex. In this locus nine unique first exons encoding NH2-terminal portions of each isoform were located at intervals of approximately 10 kb and followed by only one ...

journal_title：Journal of biochemistry

authors： Emi Y,Ikushiro S,Iyanagi T

更新日期：1995-02-01 00:00:00

abstract：:Serine dehydrogenase from Escherichia coli is a homotetrameric enzyme belonging to the short-chain dehydrogenase/reductase (SDR) family. This enzyme catalyses the NADP(+)-dependent oxidation of serine to 2-aminomalonate semialdehyde. The enzyme shows a stereospecificity for β-(3S)-hydroxy acid as a substrate; however,...

journal_title：Journal of biochemistry

authors： Yamazawa R,Nakajima Y,Mushiake K,Yoshimoto T,Ito K

更新日期：2011-06-01 00:00:00

abstract：:The crystal structure of a ternary complex of meso-2,3-butanediol dehydrogenase with NAD+ and a competitive inhibitor, mercaptoethanol, has been determined at 1.7 A resolution by means of molecular replacement and refined to a final R-factor of 0.194. The overall structure is similar to those of the other short chain ...

journal_title：Journal of biochemistry

authors： Otagiri M,Kurisu G,Ui S,Takusagawa Y,Ohkuma M,Kudo T,Kusunoki M

更新日期：2001-02-01 00:00:00

abstract：:When an aqueous solution of a spin-labeled "two tail" gangliosidoid was incubated with liposomes or sheep erythrocytes, the broad single resonance line in the ESR spectrum disappeared and a signal showing an anisotropic motion appeared, indicating that the spin-labeled "two tail" gangliosidoid in the micellar state wa...

journal_title：Journal of biochemistry

authors： Kanda S,Inoue K,Nojima S,Utsumi H,Wiegandt H

更新日期：1982-06-01 00:00:00

abstract：:Rho-kinase is a main player in the regulation of cytoskeletal events and a promising drug target in the treatment of both vascular and neurological disorders. Here we report the crystal structure of the Rho-kinase catalytic domain in complex with the specific inhibitor Y-27632. Comparison with the structure of PKA bou...

journal_title：Journal of biochemistry

authors： Yamaguchi H,Miwa Y,Kasa M,Kitano K,Amano M,Kaibuchi K,Hakoshima T

更新日期：2006-09-01 00:00:00

abstract：:Using an immunoblotting technique, we have studied the processing of plasma proteins in subcellular fractions of rat liver including rough and smooth microsomes and the Golgi subfractions. Each subcellular fraction was directly subjected to SDS-polyacrylamide gel electrophoresis and analyzed by immunoblotting with ant...

journal_title：Journal of biochemistry

authors： Oda K,Miki K,Hirose S,Takami N,Misumi Y,Ikehara Y

更新日期：1986-12-01 00:00:00

abstract：:Reports vary on the role of growth hormone (GH) in adipocyte differentiation. In this study, we showed that GH exerted dual effects depending on the stage of differentiation, using a serum-free culture of 3T3-L1 preadipocytes. GH promoted the differentiation when added to the medium during differentiation-inducing tre...

journal_title：Journal of biochemistry

authors： Tominaga S,Morikawa M,Osumi T

更新日期：2002-12-01 00:00:00

abstract：:The cytoplasmic peptide elongation factor, EF-1 alpha, is anchored at the endoplasmic reticulum membrane by phosphatidylinositol via ethanolamine bridging presumably to Asp306 of the protein. ...

journal_title：Journal of biochemistry

authors： Hayashi Y,Urade R,Utsumi S,Kito M

更新日期：1989-10-01 00:00:00

abstract：:Dynamic functionality of mitochondria is maintained by continual fusion and fission events. A mitochondrial outer membrane protein Fzo1 plays a pivotal role upon mitochondrial fusion by homo-oligomerization to tether fusing mitochondria. Fzo1 is tightly regulated by ubiquitylations and the ubiquitin-responsible AAA pr...

journal_title：Journal of biochemistry

authors： Nahar S,Chowdhury A,Ogura T,Esaki M

更新日期：2020-03-01 00:00:00

abstract：:We characterized the taurine transport system in human intestinal Caco-2 cells and showed that it is subject to adaptive regulation. The activity of taurine transport in Caco-2 cells was evaluated by means of an Na+- and Cl(-)-dependent high-affinity transport system, the characteristics of which were similar to those...

journal_title：Journal of biochemistry

authors： Satsu H,Watanabe H,Arai S,Shimizu M

更新日期：1997-06-01 00:00:00

abstract：:In this study, we demonstrated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phosphatidylserine (PS)-binding protein and localized the putative PS-binding site involved in the membrane fusion induced by this enzyme. In an attempt to identify the PS-binding proteins, we raised polyclonal antibodies against...

journal_title：Journal of biochemistry

authors： Kaneda M,Takeuchi K,Inoue K,Umeda M

更新日期：1997-12-01 00:00:00

abstract：:We investigated whether transforming growth factor (TGF)-β1 promoted epithelial-mesenchymal transition (EMT) and migration of human oral squamous cell carcinoma (hOSCC) cells. Among 6 hOSCC cell lines investigated, Smad2 phosphorylation and TGF-β target genes expression were most clearly upregulated following TGF-β1 s...

journal_title：Journal of biochemistry

authors： Saito D,Kyakumoto S,Chosa N,Ibi M,Takahashi N,Okubo N,Sawada S,Ishisaki A,Kamo M

更新日期：2013-03-01 00:00:00

abstract：:Rhizopus delemar (ATCC 34612) lipase was modified by phospholipid (PL)-treatment so as to enhance its activity on lipoprotein. In order to detect change in lipase conformation in the modified state, a preliminary experiment was performed to remove PL from the PL-treated lipase solution which included PL nonessential t...

journal_title：Journal of biochemistry

authors： Iwai M,Shimada Y,Tsujisaka Y

更新日期：1980-08-01 00:00:00

abstract：:ISG15 is a ubiquitin-like protein that is upregulated on treatment with interferon. ISG15 is considered to be covalently conjugated to cellular proteins through a sequential reaction similar to that of the ubiquitin conjugation system consisting of E1/E2/E3 enzymes: UBE1L and UbcH8 have been reported to function as E1...

journal_title：Journal of biochemistry

authors： Takeuchi T,Iwahara S,Saeki Y,Sasajima H,Yokosawa H

更新日期：2005-12-01 00:00:00

abstract：:Acetohydroxy acid synthetase [EC 4.1.3.18] of Neurospora crassa has been solubilized from a mitochondrial pellet fraction by sonic treatment in 1.0 M potassium phosphate buffer at pH 7.5 containing 10 mM MgSO4 and centrifugation at 180,000 X g for 20 min. The soluble enzyme thus obtained has a high specific activity a...

journal_title：Journal of biochemistry

authors： Kuwana H,Date M

更新日期：1975-01-01 00:00:00

abstract：:Arginine methylation in RNA-binding proteins containing arginine- and glycine-rich RGG motifs is catalyzed by specific protein arginine N-methyltransferase in cells. We previously showed that lymphoblastoid cells grown in the presence of an indirect methyltransferase inhibitor, adenosine dialdehyde (AdOx), accumulated...

journal_title：Journal of biochemistry

authors： Lin CH,Hsieh M,Li YC,Li SY,Pearson DL,Pollard KM,Li C

更新日期：2000-09-01 00:00:00

abstract：:ATPase of 14S dynein, extracted from spermatozoa of the sea urchin, Hemicentrotus pulcherrimus, and partially purified by sucrose density gradient centrifugation, was inhibited non-competitively by palmitoyl CoA at concentrations higher than 20 microns, and was stimulated at concentrations between 2 microns and 10 mic...

journal_title：Journal of biochemistry

authors： Fujiwara A,Yokokawa M,Hino A,Yasumasu I

更新日期：1982-08-01 00:00:00

abstract：:The structure and expression of the pentraxins, serum amyloid P component (SAP), and C-reactive protein (CRP), have been investigated in the guinea pig. Northern blot analysis of hepatic RNA from animals in which acute inflammation had been induced by intraperitoneal injection of thioglycollate established that neithe...

journal_title：Journal of biochemistry

authors： Rubio N,Sharp PM,Rits M,Zahedi K,Whitehead AS

更新日期：1993-03-01 00:00:00

abstract：:Studies were carried out on the delta 5-desaturation reaction in ergosterol biosynthesis with a particulate fraction of cell-free extract of yeast. A reduced pyridine nucleotide coenzyme and molecular oxygen were required for the reaction. It was shown that the enzyme activity is located in a fraction corresponding to...

journal_title：Journal of biochemistry

authors： Osumi T,Nishino T,Katsuki H

更新日期：1979-03-01 00:00:00

abstract：:We have recently shown that furin, a mammalian homologue of the yeast precursor-processing endoprotease Kex2, is involved in precursor cleavage at sites marked by the Arg-X-Lys/Arg-Arg motif within the constitutive secretory pathway. In this study, we analyzed molecular and enzymatic properties of furin expressed in C...

journal_title：Journal of biochemistry

authors： Hatsuzawa K,Murakami K,Nakayama K

更新日期：1992-03-01 00:00:00

abstract：:The 1H-NMR spectra of eight unsaturated disaccharides obtained by bacterial eliminase digestion of chondroitin sulfate and of heparan sulfate/heparin were recorded in order to construct an NMR data base of sulfated oligosaccharides and to investigate the effects of sulfation on the proton chemical shifts. These shifts...

journal_title：Journal of biochemistry

authors： Yamada S,Yoshida K,Sugiura M,Sugahara K

更新日期：1992-10-01 00:00:00