Chemical modification of tryptophan residues in ribonuclease from a Rhizopus sp.

abstract：:We obtained enhanced thermostability by replacing Ser161 with Cys in subtilisin E from Bacillus subtilis, a cysteine-free alkaline serine protease. The Ser161Cys mutant subtilisin E was purified from the culture supernatant of the recombinant B. subtilis in an oxidizing environment. SDS-polyacrylamide gel electrophore...

journal_title：Journal of biochemistry

authors： Takagi H,Hirai K,Wada M,Nakamori S

更新日期：2000-10-01 00:00:00

abstract：:The binding properties of dynein arms to the A- and B-tubules of outer doublets of cilia from Tetrahymena pyriformis were examined, with the following results: 1. When 30s dynein purified from Tetrahymena cilia was added to doublets deficient in dynein arms, it bound to both A- and B-tubules almost equally and formed ...

journal_title：Journal of biochemistry

authors： Takahashi M,Tonomura Y

更新日期：1978-12-01 00:00:00

abstract：:1. CD spectra showed that the fowl protamine, galline, has an unordered structure rich in reverse turns in neutral solution. Eight reverse turns were predicted to be present in the galline molecule on the basis of its amino acid sequence. Spectrophotometric analyses revealed that galline efficiently bound to DNA in 0....

journal_title：Journal of biochemistry

authors： Nakano M,Kasai K,Yoshida K,Tanimoto T,Tamaki Y,Tobita T

更新日期：1989-01-01 00:00:00

abstract：:Protein kinases are essential mediators of cellular signal transduction and are often dysregulated in disease. Among these, protein tyrosine kinases (PTKs) have received specific interest due to their common roles in various diseases including cancer, and emerging observations indicating that PTK signalling pathways a...

journal_title：Journal of biochemistry

authors： Dustin CM,Heppner DE,Lin MJ,van der Vliet A

更新日期：2020-02-01 00:00:00

abstract：:Human colostral macrophages stimulated by opsonized zymosan or phorbol myristate acetate (PMA) released superoxide anions (O2-) and hydrogen peroxide (H2O2) with activities comparable to those of monocytes and about one-fourth of those of polymorphonuclear leukocytes (PMNL) of blood. The O2- -forming oxidase in the ma...

journal_title：Journal of biochemistry

authors： Tsuda H,Takeshige K,Shibata Y,Minakami S

更新日期：1984-05-01 00:00:00

abstract：:A large imprinted gene cluster in human chromosome 11p15.5 has been implicated in Beckwith-Wiedemann syndrome and Wilms' tumor. We have identified a paternally expressed imprinted gene, PEG8/IGF2AS, in this locus. It is transcribed in the opposite direction to the IGF2 transcripts and some genomic regions are shared w...

journal_title：Journal of biochemistry

authors： Okutsu T,Kuroiwa Y,Kagitani F,Kai M,Aisaka K,Tsutsumi O,Kaneko Y,Yokomori K,Surani MA,Kohda T,Kaneko-Ishino T,Ishino F

更新日期：2000-03-01 00:00:00

abstract：:Nascent peptides on free and bound ribosomes prepared from rat liver were labeled and released with [3H]puromycin, and the amounts of the nascent peptides of two secretory proteins, serum albumin and transferrin, were determined by immunoprecipitation with specific antibodies. An appreciable amount of serum albumin na...

journal_title：Journal of biochemistry

authors： Oda T,Ito A

更新日期：1981-05-01 00:00:00

abstract：:The present investigation was undertaken to see to what extent the alpha-amino group of the amino acid, the side chain of the amino acid of aminoacyl-tRNA, and the tRNA structure are involved in determining the affinity of aminoacyl-tRNA for bacterial elongation factor Tu-GTP complex. Various aminoacyl-tRNAs, mis-amin...

journal_title：Journal of biochemistry

authors： Tanada S,Kawakami M,Nishio K,Takemura S

更新日期：1982-01-01 00:00:00

abstract：:Amino acid sequences of fibrinopeptides A and B from the macaque, Macaca fuscata (Japanese monkey) and the guenon, Erythrocebus patas (patas monkey) were established. Fibrinopeptides A of the monkeys had a sequence identical with those of baboons: Ala-Asp-Thr-Gly-Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg. Fibrin...

journal_title：Journal of biochemistry

authors： Nakamura S,Takenaka O,Takahashi K

更新日期：1985-05-01 00:00:00

abstract：:To clarify the properties and functions of a trypsin inhibitor from Japanese barley in comparison with the inhibitor from Pirkka barley, an inhibitor was isolated from the barley Hordeum distichum L var. emend Lamark by extraction with 1% NaCl, ammonium sulfate fractionation and repeated chromatography on DEAE-cellul...

journal_title：Journal of biochemistry

authors： Ogiso T,Noda T,Sako Y,Kato Y,Aoyama M

更新日期：1975-07-01 00:00:00

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00

abstract：:The relationship between DNA structure of replacement vectors and gene targeting efficiency was studied using positive-negative selection. The vectors contained pBR322 DNA, a bacterial neomycin-resistance gene (neo) for positive selection, a herpes simplex virus (HSV) thymidine kinase gene (tk) for negative selection,...

journal_title：Journal of biochemistry

authors： Horie K,Nishiguchi S,Maeda S,Shimada K

更新日期：1994-03-01 00:00:00

abstract：:We recently found that budding yeast Saccharomyces cerevisiae sigma1278b, but not genome project strain S288C, has a gene conferring resistance to L-azetidine-2-carboxylic acid (AZC), a toxic four-membered ring analogue of L-proline. Also, the gene, designated as MPR1, encodes a novel acetyltransferase that detoxifies...

journal_title：Journal of biochemistry

authors： Nomura M,Nakamori S,Takagi H

更新日期：2003-01-01 00:00:00

abstract：:In porcine aortic smooth muscle cells, neuropeptide Y (NPY) stimulates mobilization of CA(2+) from intracellular store sites via Y1 receptors. However, it has been debated whether or not Ca(2+) mobilization by Y1 receptors depends on the generation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] following activation of...

journal_title：Journal of biochemistry

authors： Shigeri Y,Nakajima S,Fujimoto M

更新日期：1995-09-01 00:00:00

abstract：:The immunological properties of gamma-glutamyltransferases (gamma-GTs) from human serum, liver and tonsil were studied by using a monospecific antibody to human kidney gamma-GT for the purpose of elucidating their isozymic relationships. gamma-GTs partially purified from liver and tonsil were indistinguishable in this...

journal_title：Journal of biochemistry

authors： Tsuchida S,Imai F,Sato K

更新日期：1981-03-01 00:00:00

abstract：:Ion-exchange column-purified I-protein was labeled by fluorescein isothiocyanate (FITC) at an equimolar ratio. When FITC-labeled I-protein was reacted with glycerinated myofibrils of chicken breast muscle in a phosphate-buffered saline, fluorescence was observed at the A-band and/or the Z-line of the sarcomere. Howeve...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1988-02-01 00:00:00

abstract：:Matriptase is a type II transmembrane serine protease expressed abundantly in the epithelial cells and keratinocyte. It plays a key role in the establishment and maintenance of epithelial integrity. Matriptase is considered to be at the most upstream in cellular protease cascade. Activation of its zymogen is the most ...

journal_title：Journal of biochemistry

authors： Kojima K,Inouye K

更新日期：2011-08-01 00:00:00

abstract：:Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev trans-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show the full HIV-1 regulator of virion expressio...

journal_title：Journal of biochemistry

authors： Kimura T,Ohyama A

更新日期：1994-05-01 00:00:00

abstract：:Nitrate reductase (NaR) linked to reduced methyl viologen from Clostridium perfringens was purified by ammonium sulfate precipitation. DEAE-cellulose chromatography, disc electrophoresis on polyacrylamide gel, and triple DEAE-Sephadex chromatography. The specific activity was increased 1,200-fold with a yield of 9%. T...

journal_title：Journal of biochemistry

authors： Seki-Chiba S,Ishimoto M

更新日期：1977-12-01 00:00:00

abstract：:The three-dimensional structure of cytochrome c-553 isolated from sulfate-reducing bacterium, Desulfovibrio vulgaris Miyazaki F strain, has been determined by the multi-wavelength anomalous dispersion technique with use of synchrotron radiation. The result shows that bacterial S-class cytochromes c have a variety of f...

journal_title：Journal of biochemistry

authors： Nakagawa A,Higuchi Y,Yasuoka N,Katsube Y,Yagi T

更新日期：1990-11-01 00:00:00

abstract：:In our recent study on monoclonal antibodies (mAbs AHA-1-5) against glutaraldehyde (GA)-conjugated histamine (HA), we identified one mAb (AHA-2) which can detect neuronal HA in the rat brain with an immunocytochemistry method (ICC) [Fujiwara et al. (1999) J. Biochem. 126, 503-509]. In the present study the specificity...

journal_title：Journal of biochemistry

authors： Fujiwara K,Murata I,Yagisawa S,Tanabe T,Yabuuchi M,Sakakibara R,Tsuru D

更新日期：1999-12-01 00:00:00

abstract：:In order to examine the possibility of the use of a surface plasmon resonance (SPR) sensor for real-time monitoring of the process of refolding of immobilized proteins, the refolding of firefly luciferase immobilized on a carboxymethyldextran matrix layer was analyzed. The SPR signal of the immobilized luciferase decr...

journal_title：Journal of biochemistry

authors： Zako T,Harada K,Mannen T,Yamaguchi S,Kitayama A,Ueda H,Nagamune T

更新日期：2001-01-01 00:00:00

abstract：:The modification of proteins by SUMO (small ubiquitin-like modifier) regulates various cellular processes. Sumoylation often occurs on a specific lysine residue within the consensus motif psiKxE/D. However, little is known about the specificity and selectivity of SUMO target sites. We describe here a SUMO assay with p...

journal_title：Journal of biochemistry

authors： Schwamborn K,Knipscheer P,van Dijk E,van Dijk WJ,Sixma TK,Meloen RH,Langedijk JP

更新日期：2008-07-01 00:00:00

abstract：:Calmodulin-dependent protein kinase IV (CaM-kinase IV) kinase was recently discovered in the rat brain by its activity to activate the inactive recombinant CaM-kinase IV expressed in Escherichia coli [Okuno, S. and Fujisawa, H. (1993) J. Biochem. 114, 167-170]. In the present study, CaM-kinase IV kinase was purified a...

journal_title：Journal of biochemistry

authors： Okuno S,Kitani T,Fujisawa H

更新日期：1994-10-01 00:00:00

abstract：:N tau-Methylhistidine(3-methylhistidine) in urine of the rat is mainly derived from the degradation of actin and myosin in skeletal muscle, intestine and skin. The fractional degradation rates of the myosin-actin pools of these tissues were calculated from the time course of increase in the specific radioactivities of...

journal_title：Journal of biochemistry

authors： Nagasawa T,Funabiki R

更新日期：1981-04-01 00:00:00

abstract：:Adult rat hepatocytes in primary culture cannot survive more than 2 days in the absence of calf serum. An extract of bovine pituitary gland had a similar effect to calf serum on the survival of hepatocytes, but its specific activity was 70 times that of calf serum. The survival factor was purified from bovine pituitar...

journal_title：Journal of biochemistry

authors： Asami O,Nakamura T,Mura T,Ichihara A

更新日期：1984-02-01 00:00:00

abstract：:RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

journal_title：Journal of biochemistry

authors： Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

更新日期：2020-11-01 00:00:00

abstract：:Dupuytren's disease is a palmar fibromatosis associated with changes in fibroblast activity that also affect the metabolism of extracellular matrix components. In contrast to disease connected alterations in collagen and non-collagenous glycoproteins (mainly fibronectin), the metabolism of proteoglycans, being glycosa...

journal_title：Journal of biochemistry

authors： Koźma EM,Olczyk K,Wisowski G,Głowacki A,Bobiński R

更新日期：2005-04-01 00:00:00

abstract：:Methylobacterium extorquens AM1 is an aerobic facultative methylotroph known to secrete pyrroloquinoline quinone (PQQ), a cofactor of a number of bacterial dehydrogenases, into the culture medium. To elucidate the molecular mechanism of PQQ biosynthesis, we are focusing on PqqE which is believed to be the enzyme catal...

journal_title：Journal of biochemistry

authors： Saichana N,Tanizawa K,Pechoušek J,Novák P,Yakushi T,Toyama H,Frébortová J

更新日期：2016-01-01 00:00:00

abstract：:The Ca2+-ATPase of sarcoplasmic reticulum from rabbit skeletal muscle was incorporated into vesicles made from dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine. The Ca2+-ATPase activity of these reconstituted membranes became appreciable above 20 degrees C and 30 degrees C, respectively, in accord with...

journal_title：Journal of biochemistry

authors： Higashi K,Kirino Y

更新日期：1983-12-01 00:00:00