RNF8 induces autophagy and reduces inflammation by promoting AKT degradation via ubiquitination in ulcerative colitis mice.

abstract：:Phospholipid composition of biological membranes differs between the cytoplasmic and exoplasmic leaflets. The type 4 P-type ATPases are phospholipid flippases that generate such membrane phospholipid asymmetry. Drs2p, a flippase in budding yeast, is involved in the endocytic recycling pathway. Drs2p is implicated in c...

journal_title：Journal of biochemistry

authors： Hanamatsu H,Fujimura-Kamada K,Yamamoto T,Furuta N,Tanaka K

更新日期：2014-01-01 00:00:00

abstract：:The traditional two-step EGTA/collagenase method is widely used in studying nitric oxide (NO) production in hepatocytes. The present study first revealed that hepatocytes isolated by this method spontaneously express an iNOS mRNA. Thereafter, based on this novel finding, we characterized the expression and regulation ...

journal_title：Journal of biochemistry

authors： Wang H,Gao X,Fukumoto S,Tademoto S,Sato K,Hirai K

更新日期：1998-11-01 00:00:00

abstract：:The effects of N,N'-dicyclohexylcarbodiimide (DCCD) on light-induced H+-transport and transmembrane electric potential (delta phi) formation were studied in the membrane vesicles of Halobacterium halobium R1M1. In accordance with our previous finding of the existence of two DCCD-binding components in vesicle membrane ...

journal_title：Journal of biochemistry

authors： Murakami N,Konishi T

更新日期：1985-10-01 00:00:00

abstract：:The role of thiols on the activation and/or stabilization of rat brain nitric oxide synthase (NOS) has been investigated. It was found that thiols are not necessary for stabilizing or protecting the protein during purification but are required during enzyme turnover for maximum activity. In the complete absence of thi...

journal_title：Journal of biochemistry

authors： Komori Y,Hyun J,Chiang K,Fukuto JM

更新日期：1995-04-01 00:00:00

abstract：:The ascorbate oxidase obtained from a microorganism, Acremonium sp. HI-25 (molecular weight, 80 kDa; monomeric protein), was studied with respect to atomic absorption, EPR, absorption spectra, circular dichroism (CD) spectra, and steady-state kinetics. The enzyme was found to be a multicopper protein, containing four ...

journal_title：Journal of biochemistry

authors： Hirose J,Sakurai T,Imamura K,Watanabe H,Iwamoto H,Hiromi K,Itoh H,Shin T,Murao S

更新日期：1994-05-01 00:00:00

abstract：:The amino acid sequences of human histones have been investigated for studies of histone evolution. The whole histone was prepared from human spleen and was separated into 3 fractions, H4+H3+H2A, H2B, and H1, by our technique of CM-cellulose chromatography. The H2B fraction was further purified by Bio-Gel P-60 chromat...

journal_title：Journal of biochemistry

authors： Ohe Y,Hayashi H,Iwai K

更新日期：1979-02-01 00:00:00

abstract：:In order to identify the cGMP-sensitive ion channel protein in frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by means of photoaffinity labeling with [3H]cGMP. We found four cGMP binding proteins with molecular weights (Mws) of 250K, 100K, 92K, and 53K. The 250K protein was an integral-mem...

journal_title：Journal of biochemistry

authors： Shinozawa T,Sokabe M,Terada S,Matsusaka H,Yoshizawa T

更新日期：1987-08-01 00:00:00

abstract：:The cytosol of human erythrocytes was found to contain a Ca2+-dependent thiol protease (calpain) and its specific inhibitor (calpastatin) by DEAE-cellulose chromatography at pH 8.0, although no proteolytic activity toward casein was detected in the unfractionated hemolysate. The protease required only 40 microM Ca2+ f...

journal_title：Journal of biochemistry

authors： Murakami T,Hatanaka M,Murachi T

更新日期：1981-12-01 00:00:00

abstract：:An acidic protein of human saliva, which we named SAP-1 previously, is now shown to be an inhibitor of several cysteine proteinases. The protein inhibited papain and ficin strongly, and stem bromelain and bovine cathepsin C partially. However, it did not inhibit either porcine cathepsin B or clostripain. The mode of t...

journal_title：Journal of biochemistry

authors： Isemura S,Saitoh E,Ito S,Isemura M,Sanada K

更新日期：1984-10-01 00:00:00

abstract：:The readthrough of premature termination codon (PTC) by ribosome sometimes produces full-length proteins. We previously reported a readthrough of PTC of glycosyltransferase gene B4GALNT1 with hereditary spastic paraplegia (HSP). Here we featured the readthrough of B4GALNT1 of two mutants, M4 and M2 with PTC by immunob...

journal_title：Journal of biochemistry

authors： Yesmin F,Bhuiyan RH,Ohmi Y,Ohkawa Y,Tajima O,Okajima T,Furukawa K,Furukawa K

更新日期：2020-08-01 00:00:00

abstract：:One of the 2 main hemoglobins of the sea snake Pelamis platurus, (the Yellow-bellied sea snake) comprising about 70% of the total hemoglobin, was separated by DEAE-Sephadex column chromatography. From results of gel filtration and iron content determination, both intact sea snake hemoglobin, and the isolated major hem...

journal_title：Journal of biochemistry

authors： Liu CS

更新日期：1975-07-01 00:00:00

abstract：:We examined the effects of growth factors on intracellular free Mg2+ concentrations ([Mg2+]i) in single Swiss 3T3 fibroblasts, using microfluorometry of a Mg(2+)-sensitive dye mag-fura-2. We had already noted an increase in [Mg2+]i after exposure to bombesin for 30-60 min [Ishijima, S., Sonoda, T., & Tatibana, M. (199...

journal_title：Journal of biochemistry

authors： Ishijima S,Tatibana M

更新日期：1994-04-01 00:00:00

abstract：:In this work we have studied the equilibrium and kinetic stability of a hyperthermophilic protein, O(6)-methylguanine-DNA methyltransferase (Tk-MGMT), and its mesophilic counterpart AdaC, in various chemical solutions. In an unfolding experiment using guanidine hydrochloride (GdnHCl), the unfolding free-energy change ...

journal_title：Journal of biochemistry

authors： Nishikori S,Shiraki K,Okanojo M,Imanaka T,Takagi M

更新日期：2004-10-01 00:00:00

abstract：:Basigin belongs to the immunoglobulin superfamily and may be related to the primordial form of the superfamily. Human basigin cDNA was isolated and sequenced, and the predicted protein structure was compared with that of mouse basigin and two related molecules, embigin and the chicken blood-brain barrier antigen HT7. ...

journal_title：Journal of biochemistry

authors： Miyauchi T,Masuzawa Y,Muramatsu T

更新日期：1991-11-01 00:00:00

abstract：:A protein, which was immunoreactive to antibody against cytochrome c oxidase, was found in the mitochondrial membrane fraction of sweet potato root tissue. The protein was associated relatively weakly with the mitochondrial inner membrane as compared with cytochrome c oxidase. It exerted no cytochrome c oxidase activi...

journal_title：Journal of biochemistry

authors： Maeshima M,Asahi T

更新日期：1981-08-01 00:00:00

abstract：:Streptomyces caespitosus neutral protease (ScNP) is one of the smallest metalloproteinase with a molecular mass of 14 kDa. Effects of solvent composition on ScNP activity were examined using a peptide substrate. The k(cat)/K(m) values of ScNP exhibited bell-shaped pH-dependence with the optimal pH of 6.4-7.0 and the p...

journal_title：Journal of biochemistry

authors： Inouye K,Shimada T,Yasukawa K

更新日期：2007-09-01 00:00:00

abstract：:The degradation rates of type I, II, and III collagens by tadpole collagenase were studied by measuring the viscosity of the solution and the contents of alpha chains and alpha A chains of collagen, using SDS-polyacrylamide gel electrophoresis followed by densitometric analysis of the separated peptide bands. An empir...

journal_title：Journal of biochemistry

authors： Hayashi T,Nakamura T,Hori H,Nagai Y

更新日期：1980-03-01 00:00:00

abstract：:A chromosomal gene, mlr6793, in Mesorhizobium loti was identified as the gene encoding 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) dehydrogenase (dismutase) involved in the degradation pathway for pyridoxine (vitamin B(6)). The homogenously purified recombinant enzyme has a molecular mass of 59.1 kDa...

journal_title：Journal of biochemistry

authors： Yokochi N,Yoshikane Y,Matsumoto S,Fujisawa M,Ohnishi K,Yagi T

更新日期：2009-04-01 00:00:00

abstract：:3-Ketovalidoxylamine A C-N lyase was purified about 900-fold from the cell-free extract of Flavobacterium saccharophilum by ammonium sulfate fractionation, column chromatography on CM cellulose and gel filtration on Sephacryl S-200. The purified enzyme was homogeneous as judged by sodium dodecyl sulfate (SDS) polyacry...

journal_title：Journal of biochemistry

authors： Takeuchi M,Asano N,Kameda Y,Matsui K

更新日期：1985-12-01 00:00:00

abstract：:Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each protein in different c...

journal_title：Journal of biochemistry

authors： Khademi F,Mostafaie A

更新日期：2010-05-01 00:00:00

abstract：:Formate dehydrogenase (EC 1.2.1.2) prepared from peas (Pisum sativum) was a two-subunit enzyme. The enzyme accelerated the formation of an NAD+-cyanide compound having an adsorption band at 330 nm. The enzyme was able to bind one NAD+ molecule per each subunit but only 1 mole of NAD+-cyanide compound was formed per ...

journal_title：Journal of biochemistry

authors： Ohyama T,Yamazaki I

更新日期：1975-04-01 00:00:00

abstract：:The time course of binding of N-ethylmaleimide (NEM) to the SR was measured at pH 7.5 in the presence or absence of ATP or ADP. The following results were obtained. 1. Both in the presence and absence of nucleotide, the ATPase [EC 3.6.1.3] activity decreased linearly with increase in the amount of NEM bound to the fra...

journal_title：Journal of biochemistry

authors： Yoshida H,Tonomura Y

更新日期：1976-03-01 00:00:00

abstract：:By use of purified RNA polymerase II, it was demonstrated that S-II, a stimulatory protein of RNA polymerase II, enhanced the frequency of initiation of transcription from discrete sites on the promoter region of the silk fibroin gene integrated in a supercoiled plasmid DNA in the presence of manganese. In the absence...

journal_title：Journal of biochemistry

authors： Sekimizu K,Horikoshi M,Natori S

更新日期：1984-12-01 00:00:00

abstract：:Many eukaryotic genes are interrupted by introns, which are removed from mRNA precursors by the RNA splicing mechanism. Although nucleotide sequences around splice sites have considerable homology among various genes, exact splice-site signals are unknown. Previously, applying the computer searching method to primary ...

journal_title：Journal of biochemistry

authors： Iida Y

更新日期：1985-04-01 00:00:00

abstract：:Bovine renal brush-border membranes were solubilized by 1.6% sodium cholate. Na+/H(+)-antiporter was recovered in the supernatant after centrifugation at 160,000 x g for 1 h and was successfully reconstituted into proteoliposomes by a cholate-dialysis procedure. The reconstituted Na+/H(+)-antiporter showed a pH-gradie...

journal_title：Journal of biochemistry

authors： Yamashita T,Kawakita M

更新日期：1992-02-01 00:00:00

abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...

journal_title：Journal of biochemistry

authors： Senshu T,Fukuda M,Ohashi M

更新日期：1978-10-01 00:00:00

abstract：:An adenosinetriphosphatase (ATPase) [EC 3.6.1.3] copurified with the DNA-dependent RNA polymerase [EC 2.7.7.6] from Escherichia coli was isolated to apparent homogeneity and some of its functional as well as structural properties were examined. Although the novel ATPase exhibited metal requirements similar to those of...

journal_title：Journal of biochemistry

authors： Ishihama A,Ikeuchi T,Matsumoto A,Yamamoto S

更新日期：1976-05-01 00:00:00

abstract：:Peptide:N-glycanase (PNGase) is the enzyme responsible for de-N-glycosylation of misfolded glycoproteins in the cytosol. Here, we report the molecular identification and characterization of PNGase (png-1, F56G4.5) from Caenorhabditis elegans. This enzyme released both high mannose- and complex-type N-glycans from glyc...

journal_title：Journal of biochemistry

authors： Kato T,Kawahara A,Ashida H,Yamamoto K

更新日期：2007-08-01 00:00:00

abstract：:The gene encoding a MutM from Desulfovibrio vulgaris (Miyazaki F) was cloned and expressed in Escherichia coli. A 5.9-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by XhoI and PvuII, contained a MutM gene and other open reading frames. The nucleotide sequence of the MutM gene indicated that the protein was c...

journal_title：Journal of biochemistry

authors： Sanada H,Nakanishi T,Inoue H,Kitamura M

更新日期：2009-04-01 00:00:00

abstract：:Methylobacterium extorquens AM1 is an aerobic facultative methylotroph known to secrete pyrroloquinoline quinone (PQQ), a cofactor of a number of bacterial dehydrogenases, into the culture medium. To elucidate the molecular mechanism of PQQ biosynthesis, we are focusing on PqqE which is believed to be the enzyme catal...

journal_title：Journal of biochemistry

authors： Saichana N,Tanizawa K,Pechoušek J,Novák P,Yakushi T,Toyama H,Frébortová J

更新日期：2016-01-01 00:00:00