Involvement of free ribosomes in the early stage of secretory protein biosynthesis in rat liver.

abstract：:Dimeric T. flavoviridis phospholipase A2 has been studied in terms of the interaction with essential Ca2+ by equilibrium gel filtration, ultraviolet difference spectroscopy, fluorescence measurements, and chemical modifications with p-bromophenacyl bromide. The subunit bound to Ca2+ with a 1:1 molar ratio and no coope...

journal_title：Journal of biochemistry

authors： Ohno M,Honda A,Tanaka S,Mohri N,Shieh TC,Kihara H

更新日期：1984-10-01 00:00:00

abstract：:A cyclodextrin glucanotransferase (CGTase) from Bacillus clarkii 7364 converts starch into gamma-cyclodextrin (gamma-CD) with high specificity. Comparison of the deduced amino acid sequence of this CGTase with those of other typical CGTases revealed that several amino acids are deleted or substituted with others at se...

journal_title：Journal of biochemistry

authors： Nakagawa Y,Takada M,Ogawa K,Hatada Y,Horikoshi K

更新日期：2006-09-01 00:00:00

abstract：:By use of purified RNA polymerase II, it was demonstrated that S-II, a stimulatory protein of RNA polymerase II, enhanced the frequency of initiation of transcription from discrete sites on the promoter region of the silk fibroin gene integrated in a supercoiled plasmid DNA in the presence of manganese. In the absence...

journal_title：Journal of biochemistry

authors： Sekimizu K,Horikoshi M,Natori S

更新日期：1984-12-01 00:00:00

abstract：:In order to stabilize human RNase 1 by introduction of an intramolecular cross-link, a mutant protein (4-118CL RNase 1), in which Arg4 and Val118 are replaced with cysteine residues and linked by a disulfide bond, was designed and expressed in Escherichia coli as inclusion bodies. The 4-118CL RNase 1 that refolded und...

journal_title：Journal of biochemistry

authors： Futami J,Tada H,Seno M,Ishikami S,Yamada H

更新日期：2000-08-01 00:00:00

abstract：:To model gene transcription kinetics, empirical fitting with the Hill function or S-system is often used. In this study, we derived an analytical expression for gene transcription rates in a manner similar to that developed for enzyme kinetics to describe the kinetics of gene transcription mediated by dimeric transcri...

journal_title：Journal of biochemistry

authors： Yang HT,Hsu CP,Hwang MJ

更新日期：2007-08-01 00:00:00

abstract：:Regulation of the enzymatic activity of heterotrimeric smooth muscle myosin phosphatase (SMMP) by MgATP was examined using phosphorylated myosin (P-myosin), heavy meromyosin (P-HMM), subfragment-1 (P-S1), and 20 kDa myosin light chain (P-MLC(20)) as substrates. The activity toward P-myosin and P-HMM was dose-dependent...

journal_title：Journal of biochemistry

authors： Sato O,Ogawa Y

更新日期：1999-10-01 00:00:00

abstract：:Monoclonal antibodies for human thrombomodulin, a cofactor for thrombin-catalyzed activation of protein C, were prepared and their epitopes characterized. All six antibodies (MFTM-1-MFTM-6) bound to an elastase-digested active fragment of thrombomodulin, which contains six consecutive EGF domains. Binding of thrombomo...

journal_title：Journal of biochemistry

authors： Hayashi T,Suzuki K

更新日期：1990-11-01 00:00:00

abstract：:The crystal structure of a ternary complex of meso-2,3-butanediol dehydrogenase with NAD+ and a competitive inhibitor, mercaptoethanol, has been determined at 1.7 A resolution by means of molecular replacement and refined to a final R-factor of 0.194. The overall structure is similar to those of the other short chain ...

journal_title：Journal of biochemistry

authors： Otagiri M,Kurisu G,Ui S,Takusagawa Y,Ohkuma M,Kudo T,Kusunoki M

更新日期：2001-02-01 00:00:00

abstract：:To investigate the fine substrate specificities of four highly purified exo-type cellulases (Exo-A from Aspergillus niger, CBHI and CBHII from Trichoderma reesei, and Ex-1 from Irpex lacteus), water-soluble substrates such as barley glucan, xyloglucan from tamarind (Tamarindus indica L.), and their oligosaccharides we...

journal_title：Journal of biochemistry

authors： Amano Y,Shiroishi M,Nisizawa K,Hoshino E,Kanda T

更新日期：1996-12-01 00:00:00

abstract：:During a large-scale in vitro translation analysis of a human full-length cDNA bank, we found many clones producing in vitro translation products showing ladder bands on a fluorogram with the equidistance of about 9 kDa at the position larger than the molecular mass expected from the open reading frame. We have analyz...

journal_title：Journal of biochemistry

authors： Iwamuro S,Saeki M,Kato S

更新日期：1999-07-01 00:00:00

abstract：:Genomic clones encoding the Drosophila aldolase gene were isolated and the organization of the gene was determined. The protein-coding region spanning nearly 3.5 kb consists of five coding exons (exon 2, 3, 4 alpha, 4 beta, and 4 gamma). The insect exon 2 corresponds to exons 2 to 7 of vertebrate aldolase genes and th...

journal_title：Journal of biochemistry

authors： Kai T,Sugimoto Y,Kusakabe T,Zhang R,Koga K,Hori K

更新日期：1992-11-01 00:00:00

abstract：:High mobility group (HMG) protein 1 contains two DNA binding motifs, called HMG1/2-boxes, linked with a linker region. The functional relationships between the two boxes and the mechanism of involvement of the linker region for effective binding of HMG1 were examined. The binding analyses of truncated HMG1 peptides wi...

journal_title：Journal of biochemistry

authors： Saito K,Kikuchi T,Shirakawa H,Yoshida M

更新日期：1999-02-01 00:00:00

abstract：:The yakC gene in Schizosaccharomyces pombe, which encodes yakC protein (YakC), a potential member of an aldo-keto reductase (AKR) family, was cloned and expressed in Escherichia coli cells. The recombinant YakC purified to homogeneity catalyzed the reduction of 2-nitrobenzaldehyde (k(cat), 44.1 s(-1), K(m), 0.185 +/- ...

journal_title：Journal of biochemistry

authors： Morita T,Huruta T,Ashiuchi M,Yagi T

更新日期：2002-10-01 00:00:00

abstract：:A simple and rapid preparation method for apoaspartate aminotransferase from Escherichia coli B was developed. A crude extract of the bacterial cells was treated batchwise with DEAE-cellulose. The enzyme fraction obtained was then applied to a pyridoxamine-Sepharose column. Apoaspartate aminotransferase was eluted wit...

journal_title：Journal of biochemistry

authors： Yagi T,Kirino J,Yamamoto S,Nozaki M

更新日期：1985-10-01 00:00:00

abstract：:Cytolethal distending toxin (CDT) is one of the exotoxins produced by Actinobacillus actinomycetemcomitans, an agent of localized aggressive periodontitis. We constructed N-terminal deletion mutants of CdtA using an Escherichia coli expression system and found that ADelta19-47, with a deletion from Asn-19 to Pro-47, s...

journal_title：Journal of biochemistry

authors： Saiki K,Gomi T,Konishi K

更新日期：2004-09-01 00:00:00

abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

journal_title：Journal of biochemistry

authors： Takeda A,Hirano K,Shiroya Y,Samejima T

更新日期：1983-04-01 00:00:00

abstract：:Do non-active-site residues participate in protein function in a more direct way than just by holding the static framework of the protein molecule? If so, how important are they? As a model to answer these questions, ATB17, which is a mutant of aspartate aminotransferase created by directed evolution, is an ideal syst...

journal_title：Journal of biochemistry

authors： Shimotohno A,Oue S,Yano T,Kuramitsu S,Kagamiyama H

更新日期：2001-06-01 00:00:00

abstract：:We previously reported that DNAs directly applicable to restriction analyses and transformation of Escherichia coli can be extracted from fungi and yeasts by use of anhydrous hydrogen fluoride (HF) under a mild condition, 5 min at 0 degrees C [Oshiro, S., Katsura, N., Kitada, K., and Gunge, N. (1987) FEBS Lett. 220, 3...

journal_title：Journal of biochemistry

authors： Oshiro S,Katsura N

更新日期：1995-03-01 00:00:00

abstract：:The molecular structure of the active site of horse heart met-cyano cytochrome c, as a function of temperature, has been investigated using 1H-NMR. A temperature dependence study of the NMR spectra revealed that one heme methyl proton resonance exhibits anti-Curie behavior, i.e., the hyperfine shift increases with inc...

journal_title：Journal of biochemistry

authors： Yamamoto Y

更新日期：1996-01-01 00:00:00

abstract：:Two novel oligosaccharides with the sialyl-Le(a) structure were isolated from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography, and structurally characterized...

journal_title：Journal of biochemistry

authors： Kitagawa H,Nakada H,Fukui S,Funakoshi I,Kawasaki T,Yamashina I,Tate S,Inagaki F

更新日期：1993-10-01 00:00:00

abstract：:We purified dipeptidyl peptidase II (DPP II) to homogeneity from rat kidney and determined its physicochemical properties, including its molecular weight, substrate specificity, and partial amino acid sequence. Furthermore, we screened a rat kidney cDNA library, isolated the DPP II cDNA and determined its structure. T...

journal_title：Journal of biochemistry

authors： Araki H,Li Y,Yamamoto Y,Haneda M,Nishi K,Kikkawa R,Ohkubo I

更新日期：2001-02-01 00:00:00

abstract：:The amino acid sequence of the mature protein of Euglena gracilis cytochrome c1 was determined by sequencing of its cDNA. A cDNA expression library was constructed from Euglena poly(A)+ RNA in phage lambda gt11 and screened with an antiserum raised against cytochrome c1 polypeptide isolated from purified E. gracilis c...

journal_title：Journal of biochemistry

authors： Mukai K,Wakabayashi S,Matsubara H

更新日期：1989-09-01 00:00:00

abstract：:Comparative substrate specificities of farnesyl pyrophosphate synthetases I and II purified from larvae of silkworm, Bombyx mori, were studied by use of the possible biosynthetic intermediates of juvenile hormones in the insect. In the presence of Mn2+ ions farnesyl pyrophosphate synthetase II showed higher activity t...

journal_title：Journal of biochemistry

authors： Koyama T,Matsubara M,Ogura K

更新日期：1985-08-01 00:00:00

abstract：:The sarcoplasmic reticulum (SR) membranes of rabbit skeletal muscle were allowed to react with N-(3-pyrene)maleimide (PMI) at pH 7 at 30 degrees C. The Ca(2+)-transporting activity of the SR membranes was reduced to 20% when PMI was bound to the extent of 1 mol/mol of Ca(2+)-transporting ATPase. The ATPase and the E-P...

journal_title：Journal of biochemistry

authors： Suzuki T,Kawakita M

更新日期：1993-08-01 00:00:00

abstract：:Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

journal_title：Journal of biochemistry

authors： Kiyonari S,Egashira Y,Ishino S,Ishino Y

更新日期：2014-05-01 00:00:00

abstract：:Streptomyces caespitosus neutral protease (ScNP) is one of the smallest metalloproteinase with a molecular mass of 14 kDa. Effects of solvent composition on ScNP activity were examined using a peptide substrate. The k(cat)/K(m) values of ScNP exhibited bell-shaped pH-dependence with the optimal pH of 6.4-7.0 and the p...

journal_title：Journal of biochemistry

authors： Inouye K,Shimada T,Yasukawa K

更新日期：2007-09-01 00:00:00

abstract：:Microvesicles (MVs) serve as vectors of nucleic-acid dissemination and are important mediators of intercellular communication. However, the functionality of packaged nucleic acids on recipient cells following transfer of MV cargo has not been clearly elucidated. This limitation is attributed to a lack of methodology a...

journal_title：Journal of biochemistry

authors： Lu JF,Pokharel D,Padula MP,Bebawy M

更新日期：2016-11-01 00:00:00

abstract：:When the membrane fraction of Saccharomyces carlsbergensis was incubated with radiolabeled isopentenyl diphosphate in the presence of farnesyl diphosphate and Mg2+, phosphorylated and free long-chain polyprenols were formed. The reaction was inhibited by EDTA and heavy metal cations. A series of non-ionic detergents w...

journal_title：Journal of biochemistry

authors： Bukhtiyarov YE,Shabalin YA,Kulaev IS

更新日期：1993-06-01 00:00:00

abstract：:A method is described for the preparation of a monomeric Fab'-beta-D-galactosidase conjugate, which is required for the development of a sensitive immunoenzymometric assay. Anti-human IgG F(ab')2 was labeled with 2,4-dinitrophenyl groups, split into Fab' by reduction and reacted with excess maleimide groups which had ...

journal_title：Journal of biochemistry

authors： Imagawa M,Hashida S,Ishikawa E,Freytag JW

更新日期：1984-12-01 00:00:00

abstract：:Two proteinaceous factors, 15K and 9K proteins, which acted together to stabilize the inactivated yeast F1F0-ATPase-inhibitor complex [Hashimoto, T., et al. (1984) J. Biochem. 95, 131-136] were hardly distinguishable from the sigma and epsilon subunits, respectively, of yeast F1-ATPase by sodium dodecyl sulfate (SDS) ...

journal_title：Journal of biochemistry

authors： Okada Y,Hashimoto T,Yoshida Y,Tagawa K

更新日期：1986-01-01 00:00:00