Monitoring of the refolding process for immobilized firefly luciferase with a biosensor based on surface plasmon resonance.

abstract：:GM2 containing NeuGc was found to be a major ganglioside in the liver of most inbred strains of mouse we have examined so far. They were DBA/2, C3H/He, C57BL/6, BALB/c, and CBA mice. However, WHT/Ht mouse does not contain a detectable amount of GM2 (NeuGc) but has GM3 containing NeuGc as the major ganglioside in the l...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Otsuka H,Sudo K,Suzuki K,Suzuki A,Yamakawa T

更新日期：1983-03-01 00:00:00

abstract：:The absorption of bile alcohols by rat small intestine was studied using in vivo and in vitro preparations. In bile duct cannulated rats, intraduodenal administered bile alcohols were certainly absorbed and excreted in the bile, although the absorption was not so efficient as for bile acids. The absorption during a 24...

journal_title：Journal of biochemistry

authors： Hoshita T,Harada N,Morita I,Kihira K

更新日期：1981-11-01 00:00:00

abstract：:Bindings of cobra venom phospholipases A2 to micelles of n-hexadecylphosphorylcholine were studied by the tryptophyl fluorescence method at 25 degrees C and ionic strength 0.1. The data were analyzed by assuming that the micellar surface has multiple binding sites for the enzyme and these sites are identical and mutua...

journal_title：Journal of biochemistry

authors： Teshima K,Ikeda K,Hamaguchi K,Hayashi K

更新日期：1983-07-01 00:00:00

abstract：:The STPR domain is a novel DNA-binding domain composed of repeats of 23 amino-acid-long peptide found in the fibroin-modulator-binding protein-1 (FMBP-1) of the silkworm Bombyx mori. Theoretical proteins having the STPR domain are highly conserved, particularly in vertebrates, but the functions are mostly unknown. In ...

journal_title：Journal of biochemistry

authors： Takiya S,Saito S,Yokoyama T,Matsumoto D,Aizawa T,Kamiya M,Demura M,Kawano K

更新日期：2009-07-01 00:00:00

abstract：:The inhibition of tau fibrillation is a potential therapeutic target for Alzheimer's and other neurodegenerative diseases. As a series of studies on inhibiting the transition of soluble monomeric tau into mature fibril, the effect of Tyr310 residue in the third repeat (R3) of the microtubule-binding domain (MBD) on th...

journal_title：Journal of biochemistry

authors： Nishiura C,Takeuchi K,Minoura K,Sumida M,Taniguchi T,Tomoo K,Ishida T

更新日期：2010-03-01 00:00:00

abstract：:Roxadustat (FG-4592, Rox) is a stabilizer for hypoxia-inducible transcription factors (HIFs), which induce production of the erythroid growth factor erythropoietin, and has been listed by the World Anti-Doping Agency as a prohibited substance for athletes since 2011. Although the detection technologies for Rox and its...

journal_title：Journal of biochemistry

authors： Saigusa D,Suzuki N,Matsumoto Y,Umeda K,Tomioka Y,Koshiba S,Yamamoto M

更新日期：2018-02-09 00:00:00

abstract：:Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

journal_title：Journal of biochemistry

authors： Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

更新日期：1981-11-01 00:00:00

abstract：:The conformation and stabilities of the CL fragment isolated from a type lambda Bence Jones protein and the fragment in which the intrachain disulfide bond had been reduced were studied by measuring CD, fluorescence, and ultraviolet absorption. The results indicated that no great conformational change occurs on reduct...

journal_title：Journal of biochemistry

authors： Goto Y,Hamaguchi K

更新日期：1979-11-01 00:00:00

abstract：:Cholesterol esterification and cholesterol ester hydrolysis in dog plasma were investigated. Esterification proceeded linearly for 60 min, and the amounts of cholesterol esterified were in the range of 0.13-0.18 mumol/ml/h. No change of acyl composition had occurred in newly formed cholesterol esters during incubation...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamo-Yamada F,Cho S,Sugano M

更新日期：1980-05-01 00:00:00

abstract：:We report a strategy for generating efficient signal transduction with unnatural heterologous receptor combinations. As previously described [Ueda, H., Kawahara, M. et al. (2000) J. Immunol. Methods 241, 159-170], chimeric receptors composed of the V(H)/V(L) domains of anti-hen egg lysozyme antibody HyHEL-10 and N-ter...

journal_title：Journal of biochemistry

authors： Kawahara M,Ueda H,Tsumoto K,Kumagai I,Mahoney W,Nagamune T

更新日期：2001-08-01 00:00:00

abstract：:We analyzed lipids extracted from human hepatoma HepG2 cells using a high performance liquid chromatograph equipped with a reversed phase column and found a compound with a mass spectrum showing certain diagnostic ion fragments of 1-methoxy-5-polyprenyl-phenol, a known intermediate of ubiquinone biosynthesis. Universa...

journal_title：Journal of biochemistry

authors： Kang DC,Takeshige K,Minakami S

更新日期：1990-12-01 00:00:00

abstract：:The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the pr...

journal_title：Journal of biochemistry

authors： Shiwa Y,Yoshikawa H,Tanaka T,Ogura M

更新日期：2015-05-01 00:00:00

abstract：:Aleuria aurantia lectin (AAL) is a protein composed of two identical subunits having no carbohydrate chain and shows sugar-binding specificity for L-fucose. Full-length cDNA encoding for the lectin has been isolated from a lambda gt11 library, screened with an antiserum directed against AAL. The cDNA clone contained 1...

journal_title：Journal of biochemistry

authors： Fukumori F,Takeuchi N,Hagiwara T,Ohbayashi H,Endo T,Kochibe N,Nagata Y,Kobata A

更新日期：1990-02-01 00:00:00

abstract：:Phospholipase A2 inhibitor (PLI) was purified from the blood plasma of Chinese Mamushi, Agkistrodon blomhoffii siniticus, by sequential chromatography on Sephadex G-200, Mono Q, and Blue-Sepharose CL-6B columns. The purified PLI was a glycoprotein with an apparent molecular mass of 75 kDa and was composed of a single ...

journal_title：Journal of biochemistry

authors： Ohkura N,Inoue S,Ikeda K,Hayashi K

更新日期：1993-04-01 00:00:00

abstract：:ATP synthases convert an electrochemical proton gradient into rotational movement to produce the ubiquitous energy currency adenosine triphosphate. Tension generated by the rotational torque is compensated by the stator. For this task, a peripheral stalk flexibly fixes the hydrophilic catalytic part F1 to the membrane...

journal_title：Journal of biochemistry

authors： Poetsch A,Berzborn RJ,Heberle J,Link TA,Dencher NA,Seelert H

更新日期：2007-03-01 00:00:00

abstract：:Rat serum carboxylesterase [carboxylic ester hydrolase, EC 3.1.1.1] was purified by ammonium sulfate precipitation, chromatography on DEAE-cellulose, QAE Sephadex A-50 and brushite, and gel filtration on Sephadex G-200. This purified enzyme was shown to be a single protein band on slab gel electrophoresis and its fina...

journal_title：Journal of biochemistry

authors： Hashinotsume M,Higashino K,Hada T,Yamamura Y

更新日期：1978-12-01 00:00:00

abstract：:In order to identify the cGMP-sensitive ion channel protein in frog rod outer segments (ROS), we analyzed cGMP binding proteins in the ROS by means of photoaffinity labeling with [3H]cGMP. We found four cGMP binding proteins with molecular weights (Mws) of 250K, 100K, 92K, and 53K. The 250K protein was an integral-mem...

journal_title：Journal of biochemistry

authors： Shinozawa T,Sokabe M,Terada S,Matsusaka H,Yoshizawa T

更新日期：1987-08-01 00:00:00

abstract：:Upon rehydration of lyophilized Escherichia coli cells with phosphate buffer containing [14C]isopentenyl pyrophosphate (IPP), 14C was incorporated into the cells. Radioactivity was found in ubiquinone-8, an unidentified precursor of ubiquinone-8, demethylmenaquinone-8 and phosphate esters of all-trans-octaprenol and c...

journal_title：Journal of biochemistry

authors： Fujisaki S,Nishino T,Katsuki H

更新日期：1986-04-01 00:00:00

abstract：:Glycerol dehydrogenase [EC 1.1.1.6] (pI 5.9) from Geotrichum candidium is effectively adsorbed in the presence of 20 mM acute buffer (pH 6.0) on either octyl-Sepharose or 10-carboxydecyl-Sepharose, among ten different kinds of n-alkyl-Sepharose derivatives tested, some of which have an amino or a carboxyl group as a t...

journal_title：Journal of biochemistry

authors： Sasaki I,Itoh N,Goto H,Yamamoto R,Tanaka H,Yamashita K,Yamashita J,Horio T

更新日期：1982-01-01 00:00:00

abstract：:RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

journal_title：Journal of biochemistry

authors： Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

更新日期：2020-11-01 00:00:00

abstract：:alphaB-Crystallin, a member of the small heat shock protein (sHSP) family, is expressed in various tissues including lens, heart, and skeletal muscle. Previously we identified the gene of HSPB2, another member of the sHSP family, located 1-kb upstream of the alphaB-crystallin gene in a head-to-head manner. In the pres...

journal_title：Journal of biochemistry

authors： Ijichi N,Tsujimoto N,Iwaki T,Fukumaki Y,Iwaki A

更新日期：2004-03-01 00:00:00

abstract：:Polysialoglycoprotein (PSGP) is a major cortical alveolus glycoprotein of rainbow trout eggs that is characterized by the attachment of a polysialic acid structure to its O-glycan chains. It has been demonstrated that the polysialic acid structure is synthesized by at least three sialyltransferases mostly localized in...

journal_title：Journal of biochemistry

authors： Asahina S,Sato C,Kitajima K

更新日期：2004-08-01 00:00:00

abstract：:In order to study the conformation of the side chain of lysine substrates bound to the active center of trypsin, two lysine analogs, cis- and trans-2,6-diamino-4-hexenoic acids (4,5-dehydrolysines) were synthesized and kinetic parameters for the hydrolysis of benzoyl methyl esters and phenylthiazolones of these analog...

journal_title：Journal of biochemistry

authors： Mizusaki K,Sugahara Y,Tsunematsu H,Makisumi S

更新日期：1986-07-01 00:00:00

abstract：:Cation specificity and effects of pH and temperature on membrane aggregation of porcine intestinal brush borders were studied in terms of the turbidity change of the membrane suspensions due to the addition of polyvalent cations. Monovalent cations (K+ and Na+) did not induce the membrane aggregation at 5 mM or below....

journal_title：Journal of biochemistry

authors： Ohyashiki T,Takeuchi M,Mohri T

更新日期：1984-03-01 00:00:00

abstract：:Actin-activated ATPase activity of myosin from Physarum polycephalum decreases when it binds Ca2+ and increases when it loses Ca2+. This Ca-inhibition is observed with phosphorylated myosin [Kohama, K. (1990) Trend, Pharmacol. Sci. 11, 433-435]. The activity of dephosphorylated myosin remained at a low level both in t...

journal_title：Journal of biochemistry

authors： Kohama K,Kohno T,Okagaki T,Shimmen T

更新日期：1991-10-01 00:00:00

abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

journal_title：Journal of biochemistry

authors： Takeda A,Hirano K,Shiroya Y,Samejima T

更新日期：1983-04-01 00:00:00

abstract：:The lifetime of the purple intermediate formed from D-amino acid oxidase [D-amino acid: O2 oxidoreductase (deaminating), EC 1.4.3.3] and a neutral D-amino acid under anaerobic conditions was measured with a series of neutral D-amino acids. The lifetime increases with increase in the number of carbons in the side chain...

journal_title：Journal of biochemistry

authors： Yagi K,Ohishi N

更新日期：1978-12-01 00:00:00

abstract：:We have isolated a gene (clone Y113G7A.11) from Caenorhabditis elegans (C. elegans), that we have designated as ceST1, and which is the only member of the cytosolic sulfotransferase (SULT) gene family present in the genome of this organism. We identified the SULT motifs of ceST1 based upon their deduced amino acid seq...

journal_title：Journal of biochemistry

authors： Hattori K,Inoue M,Inoue T,Arai H,Tamura HO

更新日期：2006-03-01 00:00:00

abstract：:The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate wa...

journal_title：Journal of biochemistry

authors： Mizutani H,Miyahara I,Hirotsu K,Nishina Y,Shiga K,Setoyama C,Miura R

更新日期：2000-07-01 00:00:00

abstract：:L-selectin is a cell adhesion molecule mediating the initial capture and subsequent rolling of leukocytes along the endothelial cells expressing L-selectin ligands. In addition to its action in adhesion, an intracellular signaling role for L-selectin has been recognized. Its cytoplasmic domain is involved in signal tr...

journal_title：Journal of biochemistry

authors： Chen C,Ba X,Xu T,Cui L,Hao S,Zeng X

更新日期：2006-08-01 00:00:00