Abstract:
:Cholesterol esterification and cholesterol ester hydrolysis in dog plasma were investigated. Esterification proceeded linearly for 60 min, and the amounts of cholesterol esterified were in the range of 0.13-0.18 mumol/ml/h. No change of acyl composition had occurred in newly formed cholesterol esters during incubation. With the addition of Na taurocholate (10 mM), complete inhibition of the esterifying activity and maximal activation of the hydrolase activity were observed. Approximately 50% of cholesterol esters present in plasma was hydrolyzed in 10 min of incubation, and the reaction was completed within 60 min. The maximal rate of hydrolysis was estimated to be 4.0-5.4 mumol/ml/h, and polyunsaturated esters were hydrolyzed more rapidly than saturated ones. The esterifying activity was detected in high density (HDL) and very high density lipoproteins (VHDL), while the hydrolytic activity was found only in VHDL. Each lipoprotein fraction served as a good substrate for hydrolysis, while HDL was the sole substrate for esterification. The optimal pH of the hydrolytic activity in VHDL lay in a broad range between 6.8 and 7.2 and the apparent Km was determined as 12.5 x 10(-3) mM for cholesteryl oleate.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Yamamoto K,Kamo-Yamada F,Cho S,Sugano Mdoi
10.1093/oxfordjournals.jbchem.a132864subject
Has Abstractpub_date
1980-05-01 00:00:00pages
1271-8issue
5eissn
0021-924Xissn
1756-2651journal_volume
87pub_type
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更新日期:1988-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00