Chemical synthesis, molecular cloning, and expression of the gene coding for the Trichosanthes trypsin inhibitor--a squash family inhibitor.

abstract：:A completely sodium dodecyl sulfate-insoluble protein was obtained from plasmodia of Physarum polycephalum. The amino acid composition of this protein was very similar to that of connectin, an elastic protein of muscle. When the protein suspension was treated with 6 M guanidine-HCl in the presence of 0.1 M 2-mercaptoe...

journal_title：Journal of biochemistry

authors： Ozaki K,Maruyama K

更新日期：1980-09-01 00:00:00

abstract：:Methylobacterium extorquens AM1 is an aerobic facultative methylotroph known to secrete pyrroloquinoline quinone (PQQ), a cofactor of a number of bacterial dehydrogenases, into the culture medium. To elucidate the molecular mechanism of PQQ biosynthesis, we are focusing on PqqE which is believed to be the enzyme catal...

journal_title：Journal of biochemistry

authors： Saichana N,Tanizawa K,Pechoušek J,Novák P,Yakushi T,Toyama H,Frébortová J

更新日期：2016-01-01 00:00:00

abstract：:Inositol 1,4,5-trisphosphate (InsP3) releases Ca2+ from the non-mitochondrial Ca2+ store site of various types of cells. To study the mechanisms of the Ca2+ release from the store site, the effect of InsP3 on the passive Ca2+ release and influx, and the active Ca2+ uptake in the presence of oxalate, was examined using...

journal_title：Journal of biochemistry

authors： Hirata M,Kukita M,Sasaguri T,Suematsu E,Hashimoto T,Koga T

更新日期：1985-06-01 00:00:00

abstract：:Serum mannan binding protein (MBP), a mannose/N-acetylglusosamine-specific lectin, is important in innate immunity. In order to elucidate the mechanism underlying the wide intra- and interracial variety in the MBP serum level, we have studied the transcriptional regulation of human MBP. Rapid amplification of cDNA end...

journal_title：Journal of biochemistry

authors： Naito H,Ikeda A,Hasegawa K,Oka S,Uemura K,Kawasaki N,Kawasaki T

更新日期：1999-12-01 00:00:00

abstract：:This paper describes a method for imaging the endogenous release of glutamate from cerebral neurons. This method is based on the reactions of glutamate oxidase and peroxidase, and on the detection of hydrogen peroxide by a fluorescent substrate of peroxidase. Glutamate has been sensitively measured in vitro in the ran...

journal_title：Journal of biochemistry

authors： Akagi Y,Hashigasako A,Degenaar P,Iwabuchi S,Hasan Q,Morita Y,Tamiya E

更新日期：2003-09-01 00:00:00

abstract：:RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

journal_title：Journal of biochemistry

authors： Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

更新日期：2020-11-01 00:00:00

abstract：:The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the pr...

journal_title：Journal of biochemistry

authors： Shiwa Y,Yoshikawa H,Tanaka T,Ogura M

更新日期：2015-05-01 00:00:00

abstract：:The dissociation of porcine erythrocyte catalase [EC 1.11.1.6] into subunits on denaturation with alkali, GuHCl and urea was investigated by following the changes in hydrodynamic properties, absorption and CD spectra in the Soret region and inactivation of the enzyme. It was found that dissociation proceeded in an "al...

journal_title：Journal of biochemistry

authors： Takeda A,Hirano K,Shiroya Y,Samejima T

更新日期：1983-04-01 00:00:00

abstract：:MF-18, one of the monoclonal antibodies generated to chicken myosin, cross-reacted with rabbit skeletal myosin subfragment-1 (S1). Utilizing an improved procedure of immuno-blotting, a decrease in reactivity of MF-18 to S1 by trinitrophenylation was observed. This indicates that the reactive lysyl residue is very clos...

journal_title：Journal of biochemistry

authors： Katayama E,Wakabayashi T,Reinach F,Masaki T,Fischman DA

更新日期：1984-03-01 00:00:00

abstract：:As a new strategy for drug discovery and development, I focus on drug re-profiling as a way to identify new treatments for diseases. In this strategy, the actions of existing medicines, whose safety and pharmacokinetic effects in humans have already been confirmed clinically and approved for use, are examined comprehe...

journal_title：Journal of biochemistry

authors： Mizushima T

更新日期：2011-05-01 00:00:00

abstract：:It is well established that promyelocytic leukaemia nuclear bodies (PML NBs) play important roles in DNA damage responses (DDR). After irradiation, PML NBs dynamically recruit or release important proteins involved in cell-cycle regulation, DNA repair and apoptosis. As PML protein is the key molecule of PML NBs' dynam...

journal_title：Journal of biochemistry

authors： Liu J,Song Y,Tian B,Qian J,Dong Y,Liu J,Liu B,Sun Z

更新日期：2010-12-01 00:00:00

abstract：:The activity and stability of mushroom tyrosinase were studied in ionic liquid (IL)-containing aqueous systems. The effect of three ILs ([BMIm][PF(6)], [BMIm][BF(4)]), and [BMIm][MeSO(4)], where [BMIm] = 1-butyl-3-methylimidazolium) and their inorganic salts (KMeSO(4), KPF(6), and NaBF(4)) on the enzyme performance wa...

journal_title：Journal of biochemistry

authors： Yang Z,Yue YJ,Huang WC,Zhuang XM,Chen ZT,Xing M

更新日期：2009-03-01 00:00:00

abstract：:A 45-60 kDa Gal/GalNAc-specific macrophage lectin was found to participate in the interaction between tumor cells and tumoricidal macrophages activated by an antitumor streptococcal preparation, OK-432, and in the tumoricidal activity of the activated macrophages. The binding between OK-432-elicited activated macropha...

journal_title：Journal of biochemistry

authors： Oda S,Sato M,Toyoshima S,Osawa T

更新日期：1989-06-01 00:00:00

abstract：:A soluble enzyme preparation (20,000 X g supernatant fraction), prepared from the mycelia of wild-type Neurospora crassa, was capable of transferring [14C]glucose from UDP-[14C]glucose into both trichloroacetic acid (TCA)-soluble and TCA-insoluble macromolecule products in the absence of added primer. These reactions ...

journal_title：Journal of biochemistry

authors： Takahara H,Matsuda K

更新日期：1977-06-01 00:00:00

abstract：:The Basidiomycete ras gene possesses a pyrimidine-rich stretch (CT-motif) with a short (7 bases) mirror repeat in which its major transcription start point is contained. To analyze the tertiary structure induced by the CT/AG-biased sequence and its effect on gene expression in supercoiled plasmids in Escherichia coli,...

journal_title：Journal of biochemistry

authors： Yamazaki T,Hasebe T,Shouguchi J,Amano H,Kajiwara S,Shishido K

更新日期：1997-10-01 00:00:00

abstract：:Isoleucyl-tRNA formation and isoleucine-dependent PPi-ATP exchange catalyzed by purified isoleucyl-tRNA synthetase [EC 6.1.1.5] of Escherichia coli were studied in the presence of various amounts of either Mg2+, Ca2+, Fe2+, Ni2+, or Cu2+. In the presence of Mg2+, isoleucine-dependent PPi-ATP exchange was observed in p...

journal_title：Journal of biochemistry

authors： Takeda Y,Ohnishi T,Ogiso Y

更新日期：1976-09-01 00:00:00

abstract：:beta1,4-galactosyltransferase II (beta1,4GalT II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Previously, we have reported that beta1,4GalT II overexpression increased cisplatin-induced HeLa cell apoptosis. However, the mechanisms of its expression regula...

journal_title：Journal of biochemistry

authors： Zhou J,Wei Y,Liu D,Ge X,Zhou F,Jiang XY,Gu J

更新日期：2008-04-01 00:00:00

abstract：:A fluorescent fragment of Mr = 23,800 was obtained by the papain digestion of N-iodoacetyl-N'-(5-sulfo-1-naphthyl)ethylene diamine (abbreviated as IAEDANS)-modified chicken gizzard myosin. The fragment was isolated by gel filtration on a Sephadex G-100 column in the presence of 5 M guanidine-HCl followed by anion exch...

journal_title：Journal of biochemistry

authors： Onishi H,Maita T,Miyanishi T,Watanabe S,Matsuda G

更新日期：1986-12-01 00:00:00

abstract：:The cDNA of a novel protein, which contains the association domain of alpha isoform of calmodulin-dependent protein kinase II (CaM-kinase II alpha), was cloned from rat skeletal muscle. This protein, called alpha KAP, consisted of 200 amino acid residues with a molecular weight of 22,583. alpha KAP has a highly hydrop...

journal_title：Journal of biochemistry

authors： Sugai R,Takeuchi M,Okuno S,Fujisawa H

更新日期：1996-10-01 00:00:00

abstract：:Sliding machines composed of F-actin and myosin or microtubules and kinesin or dynein convert the free energy of ATP hydrolysis into sliding movements and mechanical work. Development of optical microscopy with micromanipulation techniques has made possible direct observation of single events of sliding exhibited by s...

journal_title：Journal of biochemistry

authors： Oosawa F

更新日期：1995-11-01 00:00:00

abstract：:A method is described for the fractionation of reduced and alkylated proteins of fowl feather. Fowl feather extracts were chromatographed on a Sephadex G-75 column in 4 M urea containing 1 M NaCl and separated into four fractions, GF-1, 2, 3, and 4. The elution patterns were used to compare the components of different...

journal_title：Journal of biochemistry

authors： Akahane K,Murozono S,Murayama K

更新日期：1977-01-01 00:00:00

abstract：:We cloned and sequenced the Serratia marcescens prolyl aminopeptidase (SPAP) gene. Nucleotide sequence analysis revealed an open reading frame of 951 bp, encoding a protein of 317 amino acids with a predicted molecular weight of 36,083. The expressed enzyme was purified about 90-fold on columns of Toyopearl HW65C and ...

journal_title：Journal of biochemistry

authors： Kabashima T,Kitazono A,Kitano A,Ito K,Yoshimoto T

更新日期：1997-09-01 00:00:00

abstract：:Hepatic peroxisomes and mitochondria from 20-day-old chick embryo were separated by sucrose density gradient centrifugation and the characteristics of carnitine acyltransferases in these organelles were studied. The carnitine acyltransferase activities in peroxisomes were increased markedly by the treatment of chick e...

journal_title：Journal of biochemistry

authors： Ishii S,Ishii H,Suga T

更新日期：1985-09-01 00:00:00

abstract：:MutM protein, which removes the oxidatively damaged DNA base product, 8-oxoguanine (GO), has been crystallized by means of a hanging-drop vapor-diffusion procedure using polyethyleneglycol monomethylether 2000 as a precipitant in 2-(cyclohexylamino) ethanesulfonic acid (CHES) buffer, pH 9.8. The diffraction data deriv...

journal_title：Journal of biochemistry

authors： Sugahara M,Mikawa T,Kato R,Fukuyama K,Kumasaka T,Yamamoto M,Inoue Y,Kuramitsu S

更新日期：2000-01-01 00:00:00

abstract：:Diacylglycerol acyltransferase (DGAT) was examined as a key enzyme for triacylglycerol (TG) accumulation of an oleaginous fungus, Mortierella ramanniana var. angulispora. Subcellular fractionation of the fungus showed that DGAT activity was highest in the lipid body fraction, which occupied 77% of the recovered DGAT a...

journal_title：Journal of biochemistry

authors： Kamisaka Y,Nakahara T

更新日期：1994-12-01 00:00:00

abstract：:The phospholipid mediator, platelet activating factor (PAF: 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine), was recently reported to activate macrophage-monocyte cells as well as neutrophils or platelets. PAF was incorporated into dipalmitoylphosphatidylcholine-cholesterol liposomes, and their effect on guinea pig ...

journal_title：Journal of biochemistry

authors： Hayashi H,Kudo I,Inoue K,Nomura H,Nojima S

更新日期：1985-04-01 00:00:00

abstract：:The crystal structure of an adenylate kinase from an extremophilic archaeon Aeropyrum pernix was determined in complex with full ligands, ATP-Mg2+ and AMP, at a resolution of 2.0 Å. The protein forms a trimer as found for other adenylate kinases from archaea. Interestingly, the reacting three atoms, two phosphorus and...

journal_title：Journal of biochemistry

authors： Shibanuma Y,Nemoto N,Yamamoto N,Sampei GI,Kawai G

更新日期：2020-09-01 00:00:00

abstract：:A comparative study of vertebrate ventricle tropomyosin has been carried out from the viewpoint of molecular evolution. The ventricles containing one-component tropomyosin were generally known, and in this paper those containing two components were also found in 8 species among mammals, reptiles, amphibia, and fish, b...

journal_title：Journal of biochemistry

authors： Yamane A,Hirabayashi T

更新日期：1985-05-01 00:00:00

abstract：:Protein S, a vitamin K-dependent plasma protein having Gla-residues, increases the rate of inactivation of Factor Va by activated protein C by enhancing the binding of activated protein C to phospholipid [Walker, J.F. (1981) J. Biol. Chem. 256, 11128-11131]. The present study aimed at elucidating the effect of thrombi...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Hashimoto S

更新日期：1983-09-01 00:00:00

abstract：:Several maleimide derivatives of potential usefulness as conformational probes were tested for reactivity toward SH groups of Ca2+, Mg2+-ATPase of sarcoplasmic reticulum. These include three fluorescent labels, N-(1-anilinonaphthyl-4)maleimide (ANM), N-(p-(2-benzimidazolyl)phenyl)maleimide (BIPM), and N-(7-dimethylami...

journal_title：Journal of biochemistry

authors： Yasuoka-Yabe K,Kawakita M

更新日期：1983-09-01 00:00:00