A comparative study of tropomyosin from vertebrate ventricles.

abstract：:FOXP2 is a member of the P subfamily of FOX transcription factors, the DNA-binding domain of which is the winged helix forkhead domain (FHD). In this work we show that the FOXP2 FHD is able to bind to various DNA sequences, including a novel sequence identified in this work, with different affinities and rates as dete...

journal_title：Journal of biochemistry

authors： Webb H,Steeb O,Blane A,Rotherham L,Aron S,Machanick P,Dirr H,Fanucchi S

更新日期：2017-07-01 00:00:00

abstract：:We previously reported the utilization of antisense RNA in the development of a novel immune system against RNA coliphage SP proliferation (Hirashima et al. [1986] Proc. Natl. Acad. Sci. U.S. 83, 7726-7730). We attempted to determine the most effective (i.e., those eliciting antiviral activity) sequences for targeting...

journal_title：Journal of biochemistry

authors： Hirashima A,Sawaki S,Mizuno T,Houba-Herin N,Inouye M

更新日期：1989-07-01 00:00:00

abstract：:In the nucleus of eukaryotic cells, chromatin is tethered to the nuclear envelope (NE), wherein inner nuclear membrane proteins (INMPs) play major roles. However, in Xenopus blastula, chromatin tethering to the NE depends on nuclear filamentous actin that develops in a blastula-specific manner. To investigate whether ...

journal_title：Journal of biochemistry

authors： Oda H,Kato S,Ohsumi K,Iwabuchi M

更新日期：2020-11-10 00:00:00

abstract：:Transmissible spongiform encephalopathies (TSEs), or prion diseases, are fatal infectious neurodegenerative disorders. Their causative agents are prions, which are composed of disease-associated forms of prion protein (PrP(Sc)). Naturally occurring cases of TSEs are found in several mammalian species including humans,...

journal_title：Journal of biochemistry

authors： Hagiwara K,Hara H,Hanada K

更新日期：2013-02-01 00:00:00

abstract：:The second domain (R-020) of human urinary trypsin inhibitor (UTI) exerts similar inhibitory activities on trypsin, alpha-chymotrypsin, leukocyte elastase, and plasmin to those of UTI itself, and additionally inhibits coagulation factor Xa (FXa) and plasma kallikrein, on both of which UTI has no inhibitory effect. In ...

journal_title：Journal of biochemistry

authors： Nii A,Morishita H,Yamakawa T,Matsusue T,Hirose J,Miura T,Isaji M,Horisawa Y,Sugihara K,Kanamori T

更新日期：1994-06-01 00:00:00

abstract：:An improved procedure for the purification of a lipid-containing acidophilic thermophilic bacteriophage, phiNS11, is described. The procedure involves Cs2SO4 discontinuous density gradient, Cs2SO4 equilibrium density gradient and sucrose gradient centrifugations. The phage has an icosahedral shape, 75 nm in diameter, ...

journal_title：Journal of biochemistry

authors： Sakaki Y,Yamada K,Oshima M,Oshima T

更新日期：1977-11-01 00:00:00

abstract：:The N-terminal amino acid sequences of human urinary prokallikrein and kallikrein have been determined. Their amino acid sequences are as follows. (Formula; see text) The results showed that prokallikrein comprises an additional seven amino acids at the amino terminus of the kallikrein, of which the sequence is (H2N)A...

journal_title：Journal of biochemistry

authors： Takahashi S,Irie A,Katayama Y,Ito K,Miyake Y

更新日期：1986-03-01 00:00:00

abstract：:Microvesicles (MVs) serve as vectors of nucleic-acid dissemination and are important mediators of intercellular communication. However, the functionality of packaged nucleic acids on recipient cells following transfer of MV cargo has not been clearly elucidated. This limitation is attributed to a lack of methodology a...

journal_title：Journal of biochemistry

authors： Lu JF,Pokharel D,Padula MP,Bebawy M

更新日期：2016-11-01 00:00:00

abstract：:ATP synthases convert an electrochemical proton gradient into rotational movement to produce the ubiquitous energy currency adenosine triphosphate. Tension generated by the rotational torque is compensated by the stator. For this task, a peripheral stalk flexibly fixes the hydrophilic catalytic part F1 to the membrane...

journal_title：Journal of biochemistry

authors： Poetsch A,Berzborn RJ,Heberle J,Link TA,Dencher NA,Seelert H

更新日期：2007-03-01 00:00:00

abstract：:Alginate, which is an anionic polysaccharide, is widely distributed in the cell wall of brown algae. Alginate and the products of its degradation (oligosaccharides) are used in stabilizers, thickeners and gelling agents, especially in the food industry. The degradation of alginate generally involves a combination of s...

journal_title：Journal of biochemistry

authors： Yagi H,Fujise A,Itabashi N,Ohshiro T

更新日期：2018-04-01 00:00:00

abstract：:The promoter -10 binding region of the Bacillus subtilis sigma(A) factor forms an amphiphilic alpha-helix with three conserved isoleucines located at four-residue intervals. To investigate the structural and functional roles of the three isoleucine residues, we constructed a series of sigA mutants with single and doub...

journal_title：Journal of biochemistry

authors： Liao CT,Wang WH,Yang HS,Chen JP,Chang BY

更新日期：1999-09-01 00:00:00

abstract：:The association constants of hen egg yolk riboflavin binding protein with 8-substituted riboflavins were measured using a fluorometric titration method in the range of 10-40 degrees C, and thermodynamic parameters were calculated using ordinary methods. The flavins used were riboflavins whose 8-methyl group was substi...

journal_title：Journal of biochemistry

authors： Matsui K,Sugimoto K,Kasai S

更新日期：1982-04-01 00:00:00

abstract：:We have detected in rat liver cytosol three enzymes (termed C-1, C-2, and C-3) which cleaved the RNA moiety of RNA-DNA hybrid. These enzymes were separated from each other by DEAE-Sephadex and Sephadex G-200 chromatography. C-1 and C-2 specifically act on the RNA moiety of RNA-DNA hybrid, while C-3 degrades single-str...

journal_title：Journal of biochemistry

authors： Tashiro F,Ueno Y

更新日期：1978-08-01 00:00:00

abstract：:The location and state of an essential histidyl residue in a milk-clotting acid proteases, Mucor rennin, were investigated by NMR spectroscopy. Assignment of the C2H resonance peak of the essential histidyl residue was possible by comparison of the NMR spectrum of the native enzyme with that of the photo-oxidized enzy...

journal_title：Journal of biochemistry

authors： Etoh Y,Shoun H,Ogino T,Fujiwara S,Arima K,Beppu T

更新日期：1982-06-01 00:00:00

abstract：:Effects of anion transport inhibitors on hemolysis of human erythrocytes at 200 mPa were examined. The degree of hemolysis was decreased by treating intact cells with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonate and pyridoxal 5'-phosphate (PLP) had little effect on the hem...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Matsumoto M,Kimoto E

更新日期：1995-10-01 00:00:00

abstract：:We have analyzed the manner of incorporation of bile acid into lipid bilayers and resultant perturbation of the bilayer structure with lower bile acid/lipid ratios relevant to the physiological conditions (approximately 1 mM) by 2H and 31P NMR methods, as an aid to understanding the possible role as an endogenous tumo...

journal_title：Journal of biochemistry

authors： Saitŏ H,Sugimoto Y,Tabeta R,Suzuki S,Izumi G,Kodama M,Toyoshima S,Nagata C

更新日期：1983-12-01 00:00:00

abstract：:A synchronous enzyme-reaction system using water-soluble formazan and a non-enzymatic electron mediator was developed and applied to an enzyme immunoassay (EIA). The reaction system consists of four steps: (I) dephosphorylation of NADP(+) to produce NAD(+) by alkaline phosphatase (ALP), (II) reduction of NAD(+) to pro...

journal_title：Journal of biochemistry

authors： Inouye K,Ueno I,Yokoyama S,Sakaki T

更新日期：2002-01-01 00:00:00

abstract：:We have purified L-kynurenine 3-monooxygenase from pig liver mitochondria using a procedure involving seven steps composed of (1) preparation of mitochondrial outer membrane, (2) preparation of the zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (Chaps) insoluble outer membrane mater...

journal_title：Journal of biochemistry

authors： Uemura T,Hirai K

更新日期：1998-02-01 00:00:00

abstract：:Sialoglycoprotein which exhibits inhibitory activity for hemagglutination by Hemagglutinating Virus of Japan (HVJ, Sendai virus) was isolated from the membrane of bovine erythrocytes. Purification steps for this sialoglycoprotein included extraction with lithium diiodosalicylate, phenol partition, precipitation with e...

journal_title：Journal of biochemistry

authors： Suzuki Y,Suzuki T,Matsumoto M

更新日期：1983-06-01 00:00:00

abstract：:Parathyroid hormone-related protein (PTHrP) has two different targeting signals: an N-terminal signal peptide for the endoplasmic reticulum (ER) targeting and an internal nuclear localization signal. The protein not only functions as a secretory protein, but is also found in the nucleus and/or nucleolus under certain ...

journal_title：Journal of biochemistry

authors： Amaya Y,Nakai T,Miura S

更新日期：2016-04-01 00:00:00

abstract：:The Escherichia coli strain carrying pTP 6-10 which was constructed in our previous work (Iwakura, M., et al. (1983) J. Biochem. 93, 927-930) produces more than 400-fold dihydrofolate reductase as compared with the strain without the plasmid. Dihydrofolate reductase was highly purified from the cell-free extract of th...

journal_title：Journal of biochemistry

authors： Iwakura M,Shimura Y,Sakai T,Tsuda K

更新日期：1983-09-01 00:00:00

abstract：:Proteoheteroglycan (PHG) was prepared from Neurospora crassa cells by extraction with hot water followed by cetyltrimethylammoniumbromide fractionation. The polymer was purified by DEAE-cellulose chromatography followed by gel filtrations. The PHG was fractionated into five subfractions containing carbohydrate (65-88%...

journal_title：Journal of biochemistry

authors： Nakajima T,Yoshida M,Hiura N,Matsuda K

更新日期：1984-10-01 00:00:00

abstract：:Recombinant mouse Dnmt3a and Dnmt3b were expressed in sf9 cells and purified to near homogeneity. The purified Dnmt3a and Dnmt3b gave specific activities of 1.8 +/- 0.3 and 1.3 +/- 0.1 mol/h/mol enzyme towards poly(dGdC)-poly(dGdC), respectively, which were the highest among those reported. Dnmt3a or Dnmt3b showed sim...

journal_title：Journal of biochemistry

authors： Suetake I,Miyazaki J,Murakami C,Takeshima H,Tajima S

更新日期：2003-06-01 00:00:00

abstract：:Deleting the gene for small RNA GcvB in Esherichia coli was found to increase the sensitivity to several aminoglycoside antibiotics, such as neomycin, streptomycin, kanamycin, kasugamycin and spectinomycin, at low concentrations. GcvB, conserved in gram-negative enteric bacteria, is known to negatively control the exp...

journal_title：Journal of biochemistry

authors： Muto A,Goto S,Kurita D,Ushida C,Himeno H

更新日期：2020-11-10 00:00:00

abstract：:After partial chromatographic purification of rat liver cell sap on DEAE-cellulose, including the removal of type M2 pyruvate kinase, different forms of type L pyruvate kinase were separated by chromatofocusing. Three fractions of pyruvate kinase activity were found, eluting at pH 5.0, 5.2, and 5.3, respectively. The ...

journal_title：Journal of biochemistry

authors： Ekdahl KN,Ekman P

更新日期：1984-04-01 00:00:00

abstract：:A sensitive enzyme-immunochemical staining method was developed for detection of N-glycolylneuraminic acid (NeuGc)-containing glycosphingolipids (GSLs) on silica gel thin-layer chromatography. The procedure consists of immune reaction among NeuGc-containing GSLs, affinity-purified chicken anti-NeuGc-LacCer and horsera...

journal_title：Journal of biochemistry

authors： Higashi H,Fukui Y,Ueda S,Kato S,Hirabayashi Y,Matsumoto M,Naiki M

更新日期：1984-05-01 00:00:00

abstract：:We determined the complete amino acid sequence of a novel subtilisin inhibitor, SIL15, which had been isolated from the culture supernatant of Streptomyces bikiniensis and shown to be a member of the Streptomyces subtilisin inhibitor (SSI)-like (SIL) protein family, and then identified its reactive site. SIL15 is comp...

journal_title：Journal of biochemistry

authors： Terabe M,Kojima S,Taguchi S,Momose H,Miura K

更新日期：1995-03-01 00:00:00

abstract：:1. CD spectra showed that the fowl protamine, galline, has an unordered structure rich in reverse turns in neutral solution. Eight reverse turns were predicted to be present in the galline molecule on the basis of its amino acid sequence. Spectrophotometric analyses revealed that galline efficiently bound to DNA in 0....

journal_title：Journal of biochemistry

authors： Nakano M,Kasai K,Yoshida K,Tanimoto T,Tamaki Y,Tobita T

更新日期：1989-01-01 00:00:00

abstract：:The internal structure of the recombinant yeast-derived human hepatitis B virus surface antigen vaccine particle was investigated by small-angle neutron scattering using the contrast variation method. Data were collected in aqueous buffer solutions containing 0, 40, 60, and 100% D2O in the q range of 0.005 to 0.2 A-1 ...

journal_title：Journal of biochemistry

authors： Sato M,Sato-Miyamoto Y,Kameyama K,Ishikawa N,Imai M,Ito Y,Takagi T

更新日期：1995-12-01 00:00:00

abstract：:Human liver cytosol contains multiple forms of 3 alpha-hydroxysteroid dehydrogenase and dihydrodiol dehydrogenase with hydroxysteroid dehydrogenase activity, and multiple cDNAs for the enzymes have been cloned from human liver cDNA libraries. To understand the relationship of the multiple enzyme froms to the genes, a ...

journal_title：Journal of biochemistry

authors： Deyashiki Y,Tamada Y,Miyabe Y,Nakanishi M,Matsuura K,Hara A

更新日期：1995-08-01 00:00:00