Evolutionary well-conserved region in the signal peptide of parathyroid hormone-related protein is critical for its dual localization through the regulation of ER translocation.


:Parathyroid hormone-related protein (PTHrP) has two different targeting signals: an N-terminal signal peptide for the endoplasmic reticulum (ER) targeting and an internal nuclear localization signal. The protein not only functions as a secretory protein, but is also found in the nucleus and/or nucleolus under certain conditions. PTHrP signal peptide is less hydrophobic than most signal peptides mainly due to its evolutionarily well-conserved region (QQWS). The substitution of four tandem leucine residues for this conserved region resulted in a significant inhibition of the signal peptide cleavage. At the same time, proportion of nuclear and/or nucleolar localization decreased, probably due to tethering of the protein to the ER membrane by the uncleaved mutant signal peptide. Almost complete cleavage of the signal peptide accompanied by a lack of nuclear/nucleolar localization was achieved by combining the hydrophobic h-region and an optimized sequence of the cleavage site. In addition, mutational modifications of the distribution of charged residues in and around the signal peptide affect its cleavage and/or nuclear/nucleolar localization of the protein. These results indicate that the well-conserved region in the signal peptide plays an essential role in the dual localization of PTHrP through ER targeting and/or the membrane translocation.


J Biochem


Journal of biochemistry


Amaya Y,Nakai T,Miura S




Has Abstract


2016-04-01 00:00:00














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    authors: Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

    更新日期:1981-11-01 00:00:00

  • Structural and functional characterization of haemocyanin from the anemone hermit crab Dardanus calidus.

    abstract::Oxygen-binding to haemocyanin (Hc) is generally an exothermic process, with overall enthalphy of oxygenation varying from species to species. A number of crustacean Hcs showed a null or reduced enthalphy of oxygenation, among others, the anomuran Pagurus bernhardus and Paralithodes camtscaticae possess a completely te...

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    authors: Podda G,Manconi B,Olianas A,Pellegrini M,Messana I,Mura M,Castagnola M,Giardina B,Sanna MT

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    abstract::Cytokine-induced neutrophil chemoattractant (CINC/Gro) is a chemotactic factor for neutrophils in the rat and a member of the CXC chemokine family. The refined three-dimensional structure of CINC/Gro was derived with the aid of heteronuclear magnetic resonance spectroscopy and a hybrid method of distance geometry and ...

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    pub_type: 杂志文章


    authors: Ishihara M,Kariya Y,Kikuchi H,Minamisawa T,Yoshida K

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    pub_type: 杂志文章


    authors: Komai M,Umino M,Hanai R

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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Tsurugi K,Ogata K

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    authors: Honjo M,Ishida T,Horiike K

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    更新日期:1991-03-01 00:00:00

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    authors: Murakami Y,Kamiyama S,Howlader ZH,Yamato T,Komai M,Furukawa Y

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    authors: Sone N,Kutoh E,Sato K

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    journal_title:Journal of biochemistry

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    authors: Mizutani N,Inoue M,Omori Y,Ito H,Tamiya-Koizumi K,Takagi A,Kojima T,Nakamura M,Iwaki S,Nakatochi M,Suzuki M,Nozawa Y,Murate T

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kizaki T,Takasawa T,Mizuno Y,Shiokawa H

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


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  • Fructose 2,6-bisphosphate in human erythrocytes.

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Moriyasu M,Yamamoto M,Hamasaki N

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohyama K,Tokimitsu I,Tajima S

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    pub_type: 杂志文章


    authors: Hara A,Nakagawa M,Taniguchi H,Sawada H

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    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morishima N,Ikai A

    更新日期:1987-12-01 00:00:00

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    authors: Mima J,Suzuki H,Takahashi M,Hayashi R

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    pub_type: 杂志文章


    authors: Lee S,Oneda H,Minoda M,Tanaka A,Inouye K

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