Evolutionary well-conserved region in the signal peptide of parathyroid hormone-related protein is critical for its dual localization through the regulation of ER translocation.


:Parathyroid hormone-related protein (PTHrP) has two different targeting signals: an N-terminal signal peptide for the endoplasmic reticulum (ER) targeting and an internal nuclear localization signal. The protein not only functions as a secretory protein, but is also found in the nucleus and/or nucleolus under certain conditions. PTHrP signal peptide is less hydrophobic than most signal peptides mainly due to its evolutionarily well-conserved region (QQWS). The substitution of four tandem leucine residues for this conserved region resulted in a significant inhibition of the signal peptide cleavage. At the same time, proportion of nuclear and/or nucleolar localization decreased, probably due to tethering of the protein to the ER membrane by the uncleaved mutant signal peptide. Almost complete cleavage of the signal peptide accompanied by a lack of nuclear/nucleolar localization was achieved by combining the hydrophobic h-region and an optimized sequence of the cleavage site. In addition, mutational modifications of the distribution of charged residues in and around the signal peptide affect its cleavage and/or nuclear/nucleolar localization of the protein. These results indicate that the well-conserved region in the signal peptide plays an essential role in the dual localization of PTHrP through ER targeting and/or the membrane translocation.


J Biochem


Journal of biochemistry


Amaya Y,Nakai T,Miura S




Has Abstract


2016-04-01 00:00:00














  • Detection and partial characterization of a specific plasminogen activator inhibitor in human chondrocyte cultures.

    abstract::Serum-free culture medium collected from primary monolayer cultures of human articular chondrocytes was found to inhibit human urokinase [EC] activity. Although chondrocyte culture medium contained a small amount of endothelial-type plasminogen activator inhibitor which could be demonstrated by reverse fibri...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamada H,Stephens RW,Nakagawa T,McNicol D

    更新日期:1988-12-01 00:00:00

  • Drosophila melanogaster aldolase: characterization of the isozymes alpha, beta, and gamma generated from a single gene.

    abstract::Three isozymic forms, alpha, beta, and gamma, of Drosophila melanogaster aldolase are produced from a single gene by alternative usage of the triple exons 4 (4 alpha, 4 beta, and 4 gamma) [Shaw-Lee et al. (1992) J. Biol. Chem. 267, 3959-3967; Kim et al. (1992) Mol. Cell. Biol. 12, 773-783; Kai et al. (1992) J. Biochem...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Zhang R,Kai T,Sugimoto Y,Kusakabe T,Takasaki Y,Koga K,Hori K

    更新日期:1995-07-01 00:00:00

  • Characterization of monoclonal antibodies against human prorenin profragment and identification of active prorenins in plasma.

    abstract::Monoclonal antibodies were raised against a synthetic peptide (43 amino acid residues) that corresponds to the complete profragment of human prorenin. Seven monoclonal antibodies were chosen for further characterization. Two antibodies, 2-X-C1 and 4-X-E1, reacted with the middle region and C-terminus of the profragmen...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ishizuka Y,Iizumi S,Akiyama F,Hori H,Poorman RA,Lobl TJ,Murakami K

    更新日期:1989-09-01 00:00:00

  • Intestinal absorption of bile alcohols.

    abstract::The absorption of bile alcohols by rat small intestine was studied using in vivo and in vitro preparations. In bile duct cannulated rats, intraduodenal administered bile alcohols were certainly absorbed and excreted in the bile, although the absorption was not so efficient as for bile acids. The absorption during a 24...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hoshita T,Harada N,Morita I,Kihira K

    更新日期:1981-11-01 00:00:00

  • Oxygen metabolism of human colostral macrophages: comparison with monocytes and polymorphonuclear leukocytes.

    abstract::Human colostral macrophages stimulated by opsonized zymosan or phorbol myristate acetate (PMA) released superoxide anions (O2-) and hydrogen peroxide (H2O2) with activities comparable to those of monocytes and about one-fourth of those of polymorphonuclear leukocytes (PMNL) of blood. The O2- -forming oxidase in the ma...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsuda H,Takeshige K,Shibata Y,Minakami S

    更新日期:1984-05-01 00:00:00

  • Redox process of iron-sulfur clusters of the soluble-domain of the membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F studied by resonance Raman spectroscopy.

    abstract::Resonance Raman spectra of the soluble-domain of a membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F were recorded in different oxidation states. In the oxidized state, the Raman band due to the totally symmetric stretching mode of the iron-sulfur cluster was observed at 341 cm-1, which was attributed ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Furuichi H,Ozaki Y,Niki K,Akutsu H

    更新日期:1990-11-01 00:00:00

  • Isolation and some properties of NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii.

    abstract::NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder0 452, 411, and 3...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shioi Y,Takamiya K,Nishimura M

    更新日期:1976-02-01 00:00:00

  • Mediator complex cooperatively regulates transcription of retinoic acid target genes with Polycomb Repressive Complex 2 during neuronal differentiation.

    abstract::The Mediator complex (Mediator) plays key roles in transcription and functions as the nexus for integration of various transcriptional signals. Previously, we screened for Mediator cyclin-dependent kinase (CDK)-interacting factors and identified three proteins related to chromatin regulation. One of them, SUZ12 is req...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fukasawa R,Iida S,Tsutsui T,Hirose Y,Ohkuma Y

    更新日期:2015-11-01 00:00:00

  • Purification of an active gelatinase from collagen fiber fraction of granulation tissue in rats.

    abstract::Gelatinase was extracted at 60 degrees C from the collagen fiber-rich fraction of granulation tissue induced by carrageenin in rats. A large part of the extracted gelatinase was unbound to Zn-chelating Sepharose. The unbound gelatinase gave a single band corresponding to a molecular mass of 57 kDa on SDS-substrate PAG...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakagawa H,Hirata M,Hoshino K,Sakata K,Hatakeyama S

    更新日期:1990-09-01 00:00:00

  • Purification and characterization of a glucoamylase from Aspergillus saitoi.

    abstract::1. A major glucoamylase [EC] of Aspergillus saitoi was purified by ultrafiltration followed by successive chromatography on DEAE-Sephadex, Ultrogel AcA 44 and SP-Sephadex. The purification achieved was 23-fold from crude extract with a yield of 21%. The purified enzyme, named Gluc M1, was proved homogeneous as...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takahashi T,Inokuchi N,Irie M

    更新日期:1981-01-01 00:00:00

  • Analysis of GPI-anchored proteins involved in germline stem cell proliferation in the Caenorhabditis elegans germline stem cell niche.

    abstract::Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphospha...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Rikitake M,Matsuda A,Murata D,Dejima K,Nomura KH,Abbott KL,Mitani S,Nomura K

    更新日期:2020-12-26 00:00:00

  • Allergenic potential of rice-pollen proteins: expression, immuno-cross reactivity and IgE-binding.

    abstract::Pollen proteins from several grass species have been identified and characterized as causative allergens in grass pollinosis. In contrast, allergenic potential of pollen proteins from rice, which belongs to the same Poaceae family, has not well been investigated, despite that a few clinical cases have been reported on...

    journal_title:Journal of biochemistry

    pub_type: 临床试验,杂志文章


    authors: Hirano K,Hino S,Oshima K,Okajima T,Nadano D,Urisu A,Takaiwa F,Matsuda T

    更新日期:2013-08-01 00:00:00

  • Studies on the serine proteases associated with rat liver chromatin.

    abstract::The chromatin fraction of rat liver exhibited proteolytic activity caused by serine proteases, with maximal activity at pH 8 or 10. They were analyzed by affinity labeling with [3H]diisopropylfluorophosphate followed by SDS-polyacrylamide gel electrophoresis, and partially purified by gel filtration through Sepharose ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tsurugi K,Ogata K

    更新日期:1982-11-01 00:00:00

  • Subcellular distribution of calmodulin and calmodulin-binding sites in Tetrahymena pyriformis.

    abstract::The subcellular distribution of calmodulin and particulate calmodulin-binding activity was studied in a eukaryotic protozoan, Tetrahymena pyriformis NT-1. The particulate calmodulin-binding activity was found to be localized principally in microsomes and to some extent in cilia and surface membranes called pellicles. ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nagao S,Banno Y,Nozawa Y,Sobue K,Yamazaki R,Kakiuchi S

    更新日期:1981-09-01 00:00:00

  • Roles of aggrecan, a large chondroitin sulfate proteoglycan, in cartilage structure and function.

    abstract::Aggrecan, a large aggregating proteoglycan, is one of the major structural components of cartilage. Its core protein contains three glubular domains and two glycosaminoglycan-attachment domains. These domains play various roles to maintain cartilage structure and function. An N-terminal globular domain binds hyalurona...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Watanabe H,Yamada Y,Kimata K

    更新日期:1998-10-01 00:00:00

  • The methylamine oxidizing system of Pseudomonas AM1 reconstituted with purified components.

    abstract::The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fukumori Y,Yamanaka T

    更新日期:1987-02-01 00:00:00

  • Aspartate aminotransferase from an alkalophilic Bacillus contains an additional 20-amino acid extension at its functionally important N-terminus.

    abstract::Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

    更新日期:1996-08-01 00:00:00

  • 3(20)alpha-hydroxysteroid dehydrogenase activity of monkey liver indanol dehydrogenase.

    abstract::Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hara A,Nakagawa M,Taniguchi H,Sawada H

    更新日期:1989-11-01 00:00:00

  • Microsomal aldehyde oxygenase (MALDO): purification and characterization of a cytochrome P450 isozyme responsible for oxidation of 9-anthraldehyde to 9-anthracenecarboxylic acid in monkey liver.

    abstract::Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

    更新日期:1996-04-01 00:00:00

  • A novel DNA binding protein that recognizes the methylated c-Myc binding motif.

    abstract::We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suetake I,Tajima S,Asano A

    更新日期:1995-07-01 00:00:00

  • Studies on phospholipases from Streptomyces. II. Purification and properties of Streptomyces hachijoensis phospholipase D.

    abstract::1. Phospholipase D [EC] from Streptomyces hachijoensis was purified about 570-fold by column chromatography on DEAE-cellulose and Sephadex G-50 followed by isoelectric focusing. 2. The purified preparation was found to be homogeneous both by immunodiffusion and polyacrylamide disc gel electrophoresis. 3. The i...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Okawa Y,Yamaguchi T

    更新日期:1975-08-01 00:00:00

  • Orthologues of Streptococcus pyogenes nuclease A (SpnA) and Streptococcal 5'-nucleotidase A (S5nA) found in Streptococcus iniae.

    abstract::Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5' nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. In vitro studies have shown that SpnA is a nuclease that cleaves ssDNA and dsDNA, including the DNA backbone of neutrophil extracellular traps. S5nA was ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Soh KY,Loh JMS,Proft T

    更新日期:2018-08-01 00:00:00

  • N-acetylgalactosaminyl disaccharide terminals contained in presumed carbohydrate epitopes specific to sea-squirt allergy.

    abstract::Using the methods of methylation/GLC, MS, and 1H-NMR analyses, a disaccharide structure of GalNAc alpha 1----2Fuc1---- was identified as one of the major structural units at non-reducing terminals of a highly branched and N-glycosidically linked carbohydrate on an allergenically active glycopeptide, Gp-2 (Mr ca. 8,000...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shigeta S,Ono K,Ohta M,Kobayashi Y,Oka S

    更新日期:1989-05-01 00:00:00

  • Sialylation processes in mitochondria: evidence for two distinct sialyltransferases located in the outer membrane.

    abstract::Previous studies have shown that purified mitochondrial outer membrane is able to catalyze the transfer of sialic acid from CMP-Neu5Ac to an exogenous asialoglycoprotein acceptor, asialofetuin. Considering the heterogeneity of the glycan chains borne by this glycoprotein, an investigation of mitochondrial sialyltransf...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Gasnier F,Baubichon-Cortay H,Louisot P,Gateau-Roesch O

    更新日期:1991-11-01 00:00:00

  • Solution structure of a reverse transcriptase recognition site of a LINE RNA from zebrafish.

    abstract::Long interspersed nuclear element (LINE) is known to be transposed by reverse transcription using its RNA transcript. Recognition of the 3' stem-loop of LINE RNA by its reverse transcriptase (RT) is an important step of the retrotransposition. Our previous study revealed that the second G residue (G8) in the GGAUA loo...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Otsu M,Kajikawa M,Okada N,Kawai G

    更新日期:2017-10-01 00:00:00

  • Human spleen histone H2B. Isolation and amino acid sequence.

    abstract::The amino acid sequences of human histones have been investigated for studies of histone evolution. The whole histone was prepared from human spleen and was separated into 3 fractions, H4+H3+H2A, H2B, and H1, by our technique of CM-cellulose chromatography. The H2B fraction was further purified by Bio-Gel P-60 chromat...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohe Y,Hayashi H,Iwai K

    更新日期:1979-02-01 00:00:00

  • Interaction of native and SH-modified beta-amylase of soybean with cyclohexadextrin and maltose.

    abstract::Cyclohexadextrin and maltose bound to soybean beta-amylase and affected the environments of tryptophan and tyrosine residues, producing characteristic difference spectra in the ultraviolet region. The difference spectrum produced by cyclohexadextrin, a competitive inhibitor, had peaks at 285, 292, and 299 nm, while th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mikami B,Nomura K,Morita Y

    更新日期:1983-07-01 00:00:00

  • Purification and some properties of glutathione-S-epoxide transferase from guinea pig liver.

    abstract::Glutathione-S-epoxide transferase has been purified from guinea pig liver in a homogeneous form having a sedimentation coefficient, s20,w of 3.8S, and a molecular weight of 46,000, and dissociable into subunits with a molecular weight of 25,000. The activities with four substrates, styrene oxide, naphthalene oxide, io...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hayakawa T,Myokei Y,Yagi H,Jerina DM

    更新日期:1977-08-01 00:00:00

  • The fusogenic effect of synthetic polycations on negatively charged lipid bilayers.

    abstract::The effect of synthetic polycations, polyallylamine, and polyethylenimine, on liposomes containing phosphatidylserine was investigated along with that of polylysine and divalent cations. The addition of polycations caused aggregation of sonicated vesicles composed of phosphatidylserine and phosphatidylcholine (molar r...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oku N,Yamaguchi N,Yamaguchi N,Shibamoto S,Ito F,Nango M

    更新日期:1986-10-01 00:00:00

  • The Edg family G protein-coupled receptors for lysophospholipids: their signaling properties and biological activities.

    abstract::Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are blood-borne lysophospholipids with a wide spectrum of biological activities, which include stimulation of cell growth, prevention of apoptosis, regulation of actin cytoskeleton, and modulation of cell shape, cell migration, and invasion. Activated plate...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Takuwa Y,Takuwa N,Sugimoto N

    更新日期:2002-06-01 00:00:00