Evolutionary well-conserved region in the signal peptide of parathyroid hormone-related protein is critical for its dual localization through the regulation of ER translocation.

abstract：:Serum-free culture medium collected from primary monolayer cultures of human articular chondrocytes was found to inhibit human urokinase [EC 3.4.21.31] activity. Although chondrocyte culture medium contained a small amount of endothelial-type plasminogen activator inhibitor which could be demonstrated by reverse fibri...

journal_title：Journal of biochemistry

authors： Yamada H,Stephens RW,Nakagawa T,McNicol D

更新日期：1988-12-01 00:00:00

abstract：:Three isozymic forms, alpha, beta, and gamma, of Drosophila melanogaster aldolase are produced from a single gene by alternative usage of the triple exons 4 (4 alpha, 4 beta, and 4 gamma) [Shaw-Lee et al. (1992) J. Biol. Chem. 267, 3959-3967; Kim et al. (1992) Mol. Cell. Biol. 12, 773-783; Kai et al. (1992) J. Biochem...

journal_title：Journal of biochemistry

authors： Zhang R,Kai T,Sugimoto Y,Kusakabe T,Takasaki Y,Koga K,Hori K

更新日期：1995-07-01 00:00:00

abstract：:Monoclonal antibodies were raised against a synthetic peptide (43 amino acid residues) that corresponds to the complete profragment of human prorenin. Seven monoclonal antibodies were chosen for further characterization. Two antibodies, 2-X-C1 and 4-X-E1, reacted with the middle region and C-terminus of the profragmen...

journal_title：Journal of biochemistry

authors： Ishizuka Y,Iizumi S,Akiyama F,Hori H,Poorman RA,Lobl TJ,Murakami K

更新日期：1989-09-01 00:00:00

abstract：:The absorption of bile alcohols by rat small intestine was studied using in vivo and in vitro preparations. In bile duct cannulated rats, intraduodenal administered bile alcohols were certainly absorbed and excreted in the bile, although the absorption was not so efficient as for bile acids. The absorption during a 24...

journal_title：Journal of biochemistry

authors： Hoshita T,Harada N,Morita I,Kihira K

更新日期：1981-11-01 00:00:00

abstract：:Human colostral macrophages stimulated by opsonized zymosan or phorbol myristate acetate (PMA) released superoxide anions (O2-) and hydrogen peroxide (H2O2) with activities comparable to those of monocytes and about one-fourth of those of polymorphonuclear leukocytes (PMNL) of blood. The O2- -forming oxidase in the ma...

journal_title：Journal of biochemistry

authors： Tsuda H,Takeshige K,Shibata Y,Minakami S

更新日期：1984-05-01 00:00:00

abstract：:Resonance Raman spectra of the soluble-domain of a membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F were recorded in different oxidation states. In the oxidized state, the Raman band due to the totally symmetric stretching mode of the iron-sulfur cluster was observed at 341 cm-1, which was attributed ...

journal_title：Journal of biochemistry

authors： Furuichi H,Ozaki Y,Niki K,Akutsu H

更新日期：1990-11-01 00:00:00

abstract：:NAD+ reductase of the green photosynthetic bacterium Prosthecochloris aestuarii was isolated and purified by ammonium sulfate fractionation, DEAE-cellulose column chromatography, and Sephadex G-200 gel filtration. This enzyme is an FAD-containing flavoprotein and has absorption maxima at 485 (shoulder0 452, 411, and 3...

journal_title：Journal of biochemistry

authors： Shioi Y,Takamiya K,Nishimura M

更新日期：1976-02-01 00:00:00

abstract：:The Mediator complex (Mediator) plays key roles in transcription and functions as the nexus for integration of various transcriptional signals. Previously, we screened for Mediator cyclin-dependent kinase (CDK)-interacting factors and identified three proteins related to chromatin regulation. One of them, SUZ12 is req...

journal_title：Journal of biochemistry

authors： Fukasawa R,Iida S,Tsutsui T,Hirose Y,Ohkuma Y

更新日期：2015-11-01 00:00:00

abstract：:Gelatinase was extracted at 60 degrees C from the collagen fiber-rich fraction of granulation tissue induced by carrageenin in rats. A large part of the extracted gelatinase was unbound to Zn-chelating Sepharose. The unbound gelatinase gave a single band corresponding to a molecular mass of 57 kDa on SDS-substrate PAG...

journal_title：Journal of biochemistry

authors： Nakagawa H,Hirata M,Hoshino K,Sakata K,Hatakeyama S

更新日期：1990-09-01 00:00:00

abstract：:1. A major glucoamylase [EC 3.2.1.3] of Aspergillus saitoi was purified by ultrafiltration followed by successive chromatography on DEAE-Sephadex, Ultrogel AcA 44 and SP-Sephadex. The purification achieved was 23-fold from crude extract with a yield of 21%. The purified enzyme, named Gluc M1, was proved homogeneous as...

journal_title：Journal of biochemistry

authors： Takahashi T,Inokuchi N,Irie M

更新日期：1981-01-01 00:00:00

abstract：:Stem cells divide and undergo self-renewal depending on the signals received from the stem cell niche. This phenomenon is indispensable to maintain tissues and organs in individuals. However, not all the molecular factors and mechanisms of self-renewal are known. In our previous study, we reported that glycosylphospha...

journal_title：Journal of biochemistry

authors： Rikitake M,Matsuda A,Murata D,Dejima K,Nomura KH,Abbott KL,Mitani S,Nomura K

更新日期：2020-12-26 00:00:00

abstract：:Pollen proteins from several grass species have been identified and characterized as causative allergens in grass pollinosis. In contrast, allergenic potential of pollen proteins from rice, which belongs to the same Poaceae family, has not well been investigated, despite that a few clinical cases have been reported on...

journal_title：Journal of biochemistry

authors： Hirano K,Hino S,Oshima K,Okajima T,Nadano D,Urisu A,Takaiwa F,Matsuda T

更新日期：2013-08-01 00:00:00

abstract：:The chromatin fraction of rat liver exhibited proteolytic activity caused by serine proteases, with maximal activity at pH 8 or 10. They were analyzed by affinity labeling with [3H]diisopropylfluorophosphate followed by SDS-polyacrylamide gel electrophoresis, and partially purified by gel filtration through Sepharose ...

journal_title：Journal of biochemistry

authors： Tsurugi K,Ogata K

更新日期：1982-11-01 00:00:00

abstract：:The subcellular distribution of calmodulin and particulate calmodulin-binding activity was studied in a eukaryotic protozoan, Tetrahymena pyriformis NT-1. The particulate calmodulin-binding activity was found to be localized principally in microsomes and to some extent in cilia and surface membranes called pellicles. ...

journal_title：Journal of biochemistry

authors： Nagao S,Banno Y,Nozawa Y,Sobue K,Yamazaki R,Kakiuchi S

更新日期：1981-09-01 00:00:00

abstract：:Aggrecan, a large aggregating proteoglycan, is one of the major structural components of cartilage. Its core protein contains three glubular domains and two glycosaminoglycan-attachment domains. These domains play various roles to maintain cartilage structure and function. An N-terminal globular domain binds hyalurona...

journal_title：Journal of biochemistry

authors： Watanabe H,Yamada Y,Kimata K

更新日期：1998-10-01 00:00:00

abstract：:The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

journal_title：Journal of biochemistry

authors： Fukumori Y,Yamanaka T

更新日期：1987-02-01 00:00:00

abstract：:Aspartate aminotransferase (AspAT), responsible for a minor part of the total AspAT enzymic activity in alkalophilic Bacillus circulans, was purified, its N-terminal amino acid sequence was determined, and its gene was cloned as two separate fragments. DNA sequencing showed an open reading frame of 432 amino acids (M(...

journal_title：Journal of biochemistry

authors： Battchikova N,Koivulehto M,Denesyuk A,Ptitsyn L,Boretsky Y,Hellman J,Korpela T

更新日期：1996-08-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

journal_title：Journal of biochemistry

authors： Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

更新日期：1996-04-01 00:00:00

abstract：:We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

journal_title：Journal of biochemistry

authors： Suetake I,Tajima S,Asano A

更新日期：1995-07-01 00:00:00

abstract：:1. Phospholipase D [EC 3.1.4.4] from Streptomyces hachijoensis was purified about 570-fold by column chromatography on DEAE-cellulose and Sephadex G-50 followed by isoelectric focusing. 2. The purified preparation was found to be homogeneous both by immunodiffusion and polyacrylamide disc gel electrophoresis. 3. The i...

journal_title：Journal of biochemistry

authors： Okawa Y,Yamaguchi T

更新日期：1975-08-01 00:00:00

abstract：:Streptococcus pyogenes nuclease A (SpnA) and streptococcal 5' nucleosidase A (S5nA) are two recently described virulence factors from the human pathogen S. pyogenes. In vitro studies have shown that SpnA is a nuclease that cleaves ssDNA and dsDNA, including the DNA backbone of neutrophil extracellular traps. S5nA was ...

journal_title：Journal of biochemistry

authors： Soh KY,Loh JMS,Proft T

更新日期：2018-08-01 00:00:00

abstract：:Using the methods of methylation/GLC, MS, and 1H-NMR analyses, a disaccharide structure of GalNAc alpha 1----2Fuc1---- was identified as one of the major structural units at non-reducing terminals of a highly branched and N-glycosidically linked carbohydrate on an allergenically active glycopeptide, Gp-2 (Mr ca. 8,000...

journal_title：Journal of biochemistry

authors： Shigeta S,Ono K,Ohta M,Kobayashi Y,Oka S

更新日期：1989-05-01 00:00:00

abstract：:Previous studies have shown that purified mitochondrial outer membrane is able to catalyze the transfer of sialic acid from CMP-Neu5Ac to an exogenous asialoglycoprotein acceptor, asialofetuin. Considering the heterogeneity of the glycan chains borne by this glycoprotein, an investigation of mitochondrial sialyltransf...

journal_title：Journal of biochemistry

authors： Gasnier F,Baubichon-Cortay H,Louisot P,Gateau-Roesch O

更新日期：1991-11-01 00:00:00

abstract：:Long interspersed nuclear element (LINE) is known to be transposed by reverse transcription using its RNA transcript. Recognition of the 3' stem-loop of LINE RNA by its reverse transcriptase (RT) is an important step of the retrotransposition. Our previous study revealed that the second G residue (G8) in the GGAUA loo...

journal_title：Journal of biochemistry

authors： Otsu M,Kajikawa M,Okada N,Kawai G

更新日期：2017-10-01 00:00:00

abstract：:The amino acid sequences of human histones have been investigated for studies of histone evolution. The whole histone was prepared from human spleen and was separated into 3 fractions, H4+H3+H2A, H2B, and H1, by our technique of CM-cellulose chromatography. The H2B fraction was further purified by Bio-Gel P-60 chromat...

journal_title：Journal of biochemistry

authors： Ohe Y,Hayashi H,Iwai K

更新日期：1979-02-01 00:00:00

abstract：:Cyclohexadextrin and maltose bound to soybean beta-amylase and affected the environments of tryptophan and tyrosine residues, producing characteristic difference spectra in the ultraviolet region. The difference spectrum produced by cyclohexadextrin, a competitive inhibitor, had peaks at 285, 292, and 299 nm, while th...

journal_title：Journal of biochemistry

authors： Mikami B,Nomura K,Morita Y

更新日期：1983-07-01 00:00:00

abstract：:Glutathione-S-epoxide transferase has been purified from guinea pig liver in a homogeneous form having a sedimentation coefficient, s20,w of 3.8S, and a molecular weight of 46,000, and dissociable into subunits with a molecular weight of 25,000. The activities with four substrates, styrene oxide, naphthalene oxide, io...

journal_title：Journal of biochemistry

authors： Hayakawa T,Myokei Y,Yagi H,Jerina DM

更新日期：1977-08-01 00:00:00

abstract：:The effect of synthetic polycations, polyallylamine, and polyethylenimine, on liposomes containing phosphatidylserine was investigated along with that of polylysine and divalent cations. The addition of polycations caused aggregation of sonicated vesicles composed of phosphatidylserine and phosphatidylcholine (molar r...

journal_title：Journal of biochemistry

authors： Oku N,Yamaguchi N,Yamaguchi N,Shibamoto S,Ito F,Nango M

更新日期：1986-10-01 00:00:00

abstract：:Sphingosine-1-phosphate (S1P) and lysophosphatidic acid (LPA) are blood-borne lysophospholipids with a wide spectrum of biological activities, which include stimulation of cell growth, prevention of apoptosis, regulation of actin cytoskeleton, and modulation of cell shape, cell migration, and invasion. Activated plate...

journal_title：Journal of biochemistry

authors： Takuwa Y,Takuwa N,Sugimoto N

更新日期：2002-06-01 00:00:00