Abstract:
:An improved procedure for the purification of a lipid-containing acidophilic thermophilic bacteriophage, phiNS11, is described. The procedure involves Cs2SO4 discontinuous density gradient, Cs2SO4 equilibrium density gradient and sucrose gradient centrifugations. The phage has an icosahedral shape, 75 nm in diameter, with spike-like structures radiating from the vertices. It contained about 12% by weight of lipid, 13--14% of DNA and 74--75% of protein. The phage was stable between pH 2 and 5, but rapidly inactivated above pH 6. It was highly sensitive to organic solvents and detergents. Some other basic properties such as the buoyant density and UV spectrum of the purified phage are also described.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Sakaki Y,Yamada K,Oshima M,Oshima Tdoi
10.1093/oxfordjournals.jbchem.a131833subject
Has Abstractpub_date
1977-11-01 00:00:00pages
1451-6issue
5eissn
0021-924Xissn
1756-2651journal_volume
82pub_type
杂志文章abstract::Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...
journal_title:Journal of biochemistry
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doi:10.1093/oxfordjournals.jbchem.a021227
更新日期:1996-02-01 00:00:00
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更新日期:1977-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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doi:10.1093/oxfordjournals.jbchem.a130973
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更新日期:2000-12-01 00:00:00
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更新日期:1985-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133555
更新日期:1981-10-01 00:00:00
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journal_title:Journal of biochemistry
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doi:10.1093/oxfordjournals.jbchem.a131160
更新日期:1976-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135346
更新日期:1985-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132601
更新日期:1979-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021338
更新日期:1996-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130824
更新日期:1975-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135611
更新日期:1986-05-01 00:00:00
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更新日期:1982-02-01 00:00:00
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更新日期:1988-11-01 00:00:00
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更新日期:1989-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1992-06-01 00:00:00
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更新日期:2003-07-01 00:00:00
abstract::Aleuria aurantia lectin (AAL) is a protein composed of two identical subunits having no carbohydrate chain and shows sugar-binding specificity for L-fucose. Full-length cDNA encoding for the lectin has been isolated from a lambda gt11 library, screened with an antiserum directed against AAL. The cDNA clone contained 1...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123024
更新日期:1990-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1999-02-01 00:00:00
abstract::A spectrofluorometric method for sensitive determination of metalloendopeptidase activity has been developed by using a bimane-peptide containing a tryptophan residue, i.e. 1,7-dioxo-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-alpha]pyrazol-3-yl-methyl- thiomethylcarbonyl-phenylalanyl-tryptophanyl-leucine (Bim-SCH2CO-Phe-Trp-L...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1997-12-01 00:00:00
abstract::A direct spectrophotometric suspension method for the estimation of immobilized ligands on a gel matrix was developed. The method uses 0.05% agarose solution to prepare a practically stable suspension of various agarose beads. The quantity of agarose beads in the suspension was estimated turbidometrically from the att...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131631
更新日期:1977-06-01 00:00:00
abstract::In this study, we demonstrated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phosphatidylserine (PS)-binding protein and localized the putative PS-binding site involved in the membrane fusion induced by this enzyme. In an attempt to identify the PS-binding proteins, we raised polyclonal antibodies against...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021886
更新日期:1997-12-01 00:00:00
abstract::An inhibitor of blood coagulation, a new protein with an apparent molecular weight of 34,000 and an isoelectric point of 4.9, was purified from human placental tissue by EDTA extraction. Five cDNA clones were isolated from the human placental lambda gt11 cDNA library using the mouse monoclonal antibody raised against ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122165
更新日期:1987-11-01 00:00:00
abstract::Calmodulin (CaM), a physiologically important Ca(2+)-binding protein, participates in numerous cellular regulatory processes. It is dumbbell shaped and contains two globular domains connected by a short alpha-helix. Each of the globular domains has two Ca(2+)-binding sites, the EF hands. CaM undergoes a conformational...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvp107
更新日期:2009-10-01 00:00:00