Chemical deglycosylation of hen ovomucoid: protective effect of carbohydrate moiety on tryptic hydrolysis and heat denaturation.

abstract：:The SH groups of glutamine synthetase [EC 6.3.1.2] from Bacillus stearothermophilus were modified with 5, 5'-dithiobis(2-nitrobenzoic acid) in order to determine the number of SH groups in the molecule as well as the effect of the modification on the enzyme activity. Three SH groups per subunit were detected after co...

journal_title：Journal of biochemistry

authors： Hachimori A,Takeda A,Nagaoka T,Suzuki H,Nosoh Y,Samejima T

更新日期：1975-12-01 00:00:00

abstract：:Nascent peptides on free and bound ribosomes prepared from rat liver were labeled and released with [3H]puromycin, and the amounts of the nascent peptides of two secretory proteins, serum albumin and transferrin, were determined by immunoprecipitation with specific antibodies. An appreciable amount of serum albumin na...

journal_title：Journal of biochemistry

authors： Oda T,Ito A

更新日期：1981-05-01 00:00:00

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:Affinity labeling with nucleoside polyphosphopyridoxals, especially those with 3 or 4 phosphate groups, was effective for identifying the lysyl residue(s) located at or near the binding site for ATP, GTP, UDP-Glc, or ADP-Glc in various proteins. Furthermore, kinetic analysis of the mutant enzymes, in which the labeled...

journal_title：Journal of biochemistry

authors： Fukui T

更新日期：1995-06-01 00:00:00

abstract：:Guanidine hydrochloride (GdnHCl)-induced unfolding of bovine spleen galectin-1 (Gal-1) exhibits three-state mechanism involving exclusive, structured tertiary monomer in 0.5 M GdnHCl. Gal-1 has one tryptophan residue (Trp 68) per subunit. Phosphorescence spectra of both Gal-1 dimer and intermediate monomer at 77 K sho...

journal_title：Journal of biochemistry

authors： Mandal P,Molla AR,Mandal DK

更新日期：2013-12-01 00:00:00

abstract：:Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodec...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-10-01 00:00:00

abstract：:A p-nitrophenyl-alpha-D-glucopyranoside-hydrolyzing alpha-glucosidase from an obligate thermophile, Bacillus thermoglucosidius KP 1006, gave a triphasic relationship at pH 6.8 in the van't Hoff plot of Km, in the Arrhenius plot of the first order rate constant of inactivation with 0.1% sodium dodecyl sulfate, and in t...

journal_title：Journal of biochemistry

authors： Suzuki Y,Nakamura N,Kishigami T,Abe S

更新日期：1980-03-01 00:00:00

abstract：:1) A method is described for the separation and fractionation of nonhistone chromosomal proteins from salt-urea dissociated calf thymus chromatin. After precipitating DNA in the dissociated chromatin solution with LaCl3, the chromosomal proteins in the supernatant were fractionated by SP-Sephadex C-25 column chromatog...

journal_title：Journal of biochemistry

authors： Yoshida M,Kikuchi A,Shimura K

更新日期：1975-05-01 00:00:00

abstract：:We have reported that high glucose conditions (27 mM for 4 days) induces activation of protein tyrosine phosphatases (PTPases) which are associated with impaired insulin signaling in Rat 1 fibroblasts expressing human insulin receptors [Maegawa, H. et al. (1995) J. Biol. Chem. 270, 7724-7730]. In this study, we found ...

journal_title：Journal of biochemistry

authors： Obata T,Maegawa H,Kashiwagi A,Pillay TS,Kikkawa R

更新日期：1998-05-01 00:00:00

abstract：:Physicochemical and biological properties of recombinant human erythropoietin (rhEPO) were compared with human urinary erythropoietin (uEPO). uEPO and rhEPO were purified to apparent homogeneity from the urine of patients with aplastic anemia and from the conditioned medium of Chinese hamster ovary (CHO) cells transfe...

journal_title：Journal of biochemistry

authors： Imai N,Kawamura A,Higuchi M,Oh-eda M,Orita T,Kawaguchi T,Ochi N

更新日期：1990-03-01 00:00:00

abstract：:The dephosphorylation of phospho-amino acids with alkaline phosphatase (AlPase) from calf intestine or Escherichia coli and the phosphorylation of bovine serum albumin (BSA) with epidermal growth factor (EGF) receptor kinase from human A431 epidermoid carcinoma cells were investigated by 31P NMR spectroscopy. The init...

journal_title：Journal of biochemistry

authors： Takahashi K,Shimidzu M,Shindo H,Kawamoto T,Nishi M,Matsumoto U,Taniguchi S

更新日期：1987-05-01 00:00:00

abstract：:Yeast mitochondrial ATP synthase has three regulatory proteins, ATPase inhibitor, 9K protein, and 15K protein. The 9K protein binds directly to purified F1-ATPase, as does the ATPase inhibitor, but the 15K protein does not [Hashimoto, T. et al. (1987) J. Biochem. 102, 685-692]. In the present study, we found that 15K ...

journal_title：Journal of biochemistry

authors： Hashimoto T,Yoshida Y,Tagawa K

更新日期：1990-07-01 00:00:00

abstract：:Many membrane proteins such as ion channels are oligomers, but the determinants of the degree of oligomerization are not fully understood. Mechanosensitive channel with large conductance (MscL), which is ubiquitous in bacteria, is a homopentamer with two transmembrane helices and a cytoplasmic helix in each subunit. T...

journal_title：Journal of biochemistry

authors： Ando C,Liu N,Yoshimura K

更新日期：2015-08-01 00:00:00

abstract：:The interaction between lipopolysaccharide (LPS) and an LPS-sensitive serine protease zymogen, factor C, purified from horseshoe crab (Tachypleus tridentatus) hemocytes, was investigated to elucidate the LPS-mediated activation of factor C. The rate of activation of the zymogen factor C was highly dependent on the con...

journal_title：Journal of biochemistry

authors： Nakamura T,Tokunaga F,Morita T,Iwanaga S

更新日期：1988-02-01 00:00:00

abstract：:Pig heart phosphoprotein phosphatase [phosphoprotein phosphophydrolase, EC 3.1.3.16] of Mr 224,000 was dissociated by gel-filtration on Sephacryl S-300, into an active subunit (alpha subunit) of Mr 31,000 and inactive subunits of higher molecular weight in the presence of 6 M urea. After the removal of urea, these sub...

journal_title：Journal of biochemistry

authors： Imazu M,Imaoka T,Usui H,Kinohara N,Takeda M

更新日期：1981-09-01 00:00:00

abstract：:The pcd gene from Flavobacterium lutescens IFO3084 encoding Delta'-piperideine-6-carboxylate dehydrogenase (PCD) was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence of PCD from F. lutescens IFO3084 showed strong similarity to that from Streptomyces clavuligerus. The molecular mass...

journal_title：Journal of biochemistry

authors： Fujii T,Narita T,Agematu H,Agata N,Isshiki K

更新日期：2000-12-01 00:00:00

abstract：:ZFP36L1 is an RNA-binding protein responsible for mRNA decay in the cytoplasm. ZFP36L1 has also been suggested as a nuclear-cytoplasmic shuttling protein because it contains a potential nuclear localization signal and a nuclear export signal. However, it remains unclear how the nuclear localization of ZFP36L1 is contr...

journal_title：Journal of biochemistry

authors： Matsuura Y,Noguchi A,Sakai S,Yokota N,Kawahara H

更新日期：2020-11-01 00:00:00

abstract：:Single-site catalysis by F1-ATPase from a thermophilic bacterium PS3 (TF1) was examined by incubating the enzyme with a submolar amount of radioactive ATP. The profile of single-site catalysis by TF1 at 23 degrees C was different from that of beef heart mitochondrial F1-ATPase (MF1). ATP hydrolysis on the enzyme and r...

journal_title：Journal of biochemistry

authors： Yohda M,Yoshida M

更新日期：1987-10-01 00:00:00

abstract：:Haematopoiesis and blood vessel formation are closely associated, with several molecules employed by both systems. Recently, vasohibin-1 (VASH1), an endothelium-derived negative feedback regulator of angiogenesis, has been isolated and characterized. VASH1 is induced by VEGF or bFGF in endothelial cells (ECs) and inhi...

journal_title：Journal of biochemistry

authors： Naito H,Kidoya H,Sato Y,Takakura N

更新日期：2009-05-01 00:00:00

abstract：:When crude neurofilaments were dissolved in a solution containing 8 M urea and 1% beta-mercaptoethanol (beta-ME), the component proteins of the neurofilaments and other contaminating filaments were solubilized into monomeric forms. However, when reassembled filaments were solubilized again by the addition of urea to 8...

journal_title：Journal of biochemistry

authors： Tokutake S,Tanaka K

更新日期：1989-01-01 00:00:00

abstract：:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of...

journal_title：Journal of biochemistry

authors： Jing Y,Wang M,Tang W,Qi T,Gu C,Hao S,Zeng X

更新日期：2007-05-01 00:00:00

abstract：:The localization of GTP-binding protein (G-protein) subunits, Go alpha, Gi2 alpha and beta, in subcellular fractions of rat cerebral cortex was determined by means of immunoassays specific for the respective subunits. High concentrations of all three subunits were observed in both crude mitochondrial and microsomal fr...

journal_title：Journal of biochemistry

authors： Asano T,Nagahama M,Kato K

更新日期：1990-05-01 00:00:00

abstract：:Phosphoenolpyruvate carboxylase (PEPC) [EC 4.1.1.31] has a highly conserved and unique sequence, 578-FHGRGGSIGRGGAP-591 (on Escherichia coli, PEPC), in which a GRGG motif is repeated twice with two intervening residues. Since previous chemical modification studies suggested the functional importance of arginine residu...

journal_title：Journal of biochemistry

authors： Yano M,Terada K,Umiji K,Izui K

更新日期：1995-06-01 00:00:00

abstract：:Vertebrate skeletal muscle alpha-tropomyosin polymerizes in a head-to-tail manner and binds cooperatively to actin. It has been postulated that the cooperative actin binding is governed by the strength of the head-to-tail interaction. In order to know the relationship between the head-to-tail affinity and actin bindin...

journal_title：Journal of biochemistry

authors： Sano K,Maeda K,Oda T,Maéda Y

更新日期：2000-06-01 00:00:00

abstract：:We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. Th...

journal_title：Journal of biochemistry

authors： Ema M,Matsushita N,Sogawa K,Ariyama T,Inazawa J,Nemoto T,Ota M,Oshimura M,Fujii-Kuriyama Y

更新日期：1994-10-01 00:00:00

abstract：:Acetohydroxy acid synthetase [EC 4.1.3.18] of Neurospora crassa has been solubilized from a mitochondrial pellet fraction by sonic treatment in 1.0 M potassium phosphate buffer at pH 7.5 containing 10 mM MgSO4 and centrifugation at 180,000 X g for 20 min. The soluble enzyme thus obtained has a high specific activity a...

journal_title：Journal of biochemistry

authors： Kuwana H,Date M

更新日期：1975-01-01 00:00:00

abstract：:Palmitoyl CoA inhibited EDTA-ATPase of heavy meromyosin (HMM) prepared from rabbit skeletal muscle. The concentration for half maximum inhibition of EDTA-ATPase was about 18 microM. Myristoyl CoA, the other long chain fatty acyl CoA, also inhibited EDTA-HMM ATPase, but CoA and short chain CoA thioesters, such as butyr...

journal_title：Journal of biochemistry

authors： Fujiwara A,Fujisaki H,Asai H,Yasumasu I

更新日期：1981-09-01 00:00:00

abstract：:A rapid and simple method was developed for the purification of serine hydroxymethyltransferases [EC 2.1.2.1]. The procedure involved ammonium sulfate precipitation, DEAE-cellulose column chromatography and affinity chromatography on an L-adsorbent. Through this procedure the cytosolic enzyme (s-SHMT) was purified 1,6...

journal_title：Journal of biochemistry

authors： Masuda T,Sakamoto M,Nishizaki I,Hayashi H,Yamamoto M,Wada H

更新日期：1987-03-01 00:00:00

abstract：:N omega-Phosphoarginine hydrolase from rat liver cytosol was purified to apparent homogeneity on SDS-PAGE, by employing column chromatographies on Sephadex G-75, DEAE-cellulose, QAE-Toyopearl, and glutathione-2-pyridyl-disulfide-Superose. One milligram protein of the final preparation released 4 mumol/min of inorganic...

journal_title：Journal of biochemistry

authors： Yokoyama K,Ohmori H,Kumon A

更新日期：1993-02-01 00:00:00

abstract：:1. New methods of preparing troponins from slow skeletal and cardiac muscle of the chicken have been developed. The electrophoretic mobilities of slow skeletal muscle troponin subunits were different from those of the corresponding fast skeletal muscle subunits. 2. A new method for determining the amount of divalent c...

journal_title：Journal of biochemistry

authors： Kohama K

更新日期：1979-09-01 00:00:00