Simultaneous bindings of ATPase inhibitor and 9K protein to F1F0-ATPase in the presence of 15K protein in yeast mitochondria.

abstract：:The state of association of bovine Band 3, which is a major intrinsic protein of erythrocyte membranes, was examined in nonaethyleneglycol dodecyl ether (C12E9) solution by ultracentrifugation, gel filtration, gel electrophoresis and cross-linking studies. The molecular weight of bovine Band 3 was 107,000 +/- 5% as de...

journal_title：Journal of biochemistry

authors： Nakashima H,Makino S

更新日期：1980-10-01 00:00:00

abstract：:Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC 3.1.4.34] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

journal_title：Journal of biochemistry

authors： Sawai Y,Yanokura M,Tsukada K

更新日期：1979-09-01 00:00:00

abstract：:Calphobindins (CPBs, placental annexins) are intracellular Ca(2+)- and phospholipid-dependent proteins like protein kinase C [EC 2.7.1.37]. We investigated the inhibitory effects of calphobindins on the protein kinase C activity in vitro. CPB I inhibited the protein kinase C activity for both histone phosphorylation a...

journal_title：Journal of biochemistry

authors： Shibata S,Sato H,Maki M

更新日期：1992-10-01 00:00:00

abstract：:Recently, we revealed that microtubule-associated protein (MAP) 4 isoforms, which differ in the number of repeat sequences, alter the microtubule surface properties, and we proposed a hypothesis stating that the change in the surface properties may regulate the movements of microtubule motors [Tokuraku et al. (2003) J...

journal_title：Journal of biochemistry

authors： Tokuraku K,Noguchi TQ,Nishie M,Matsushima K,Kotani S

更新日期：2007-04-01 00:00:00

abstract：:Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecul...

journal_title：Journal of biochemistry

authors： Igarashi J,Hayashi N,Kikuchi G

更新日期：1976-11-01 00:00:00

abstract：:Covalently closed-circular, superhelical DNAs, including viral DNAs, bacterial plasmid DNAs, and bacteriophage replicative-form DNA, were treated with a small amount of Haemophilus gallinarum DNA-relaxing enzyme to generate incompletely relaxed DNA molecules. Each sample consisted of a set of closed-circular DNA molec...

journal_title：Journal of biochemistry

authors： Shishido K

更新日期：1979-09-01 00:00:00

abstract：:We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted into liposomes comprising Salmonella minnesota R595 lipopolysaccharides, and fusion of spleen cells with a mouse myeloma cell...

journal_title：Journal of biochemistry

authors： Watarai S,Kushi Y,Shigeto R,Misawa N,Eishi Y,Handa S,Yasuda T

更新日期：1995-05-01 00:00:00

abstract：:Two forms of urokinase [EC 3.4.99.26] with molecular weights of 51,600 and 34,500 were purified from human urine. The specific activities of the high molecular weight urokinase (HMW-UK) and low molecular weight urokinase (LMW-UK) were 157,400 and 246,700 International Units (IU/mg), respectively. Purified HMW-UK was 9...

journal_title：Journal of biochemistry

authors： Nobuhara M,Sakamaki M,Ohnishi H,Suzuki Y

更新日期：1981-07-01 00:00:00

abstract：:Midkine (MK) and pleiotrophin (PTN) constitute a new family of heparin-binding growth factors. Extraembryonic membranes and the placenta of the mouse expressed MK mRNA at 11.5 days gestation. While the MK mRNA level in extraembryonic membranes decreased during embryogenesis, that in the placenta remained unchanged. Im...

journal_title：Journal of biochemistry

authors： Obama H,Tsutsui J,Ozawa M,Yoshida H,Yoshida Y,Osame M,Muramatsu T

更新日期：1995-07-01 00:00:00

abstract：:Molecular cloning of the gene and the crystal structure of the prolyl aminopeptidase [EC 3.4.11.5] from Serratia marcescens have been studied by us [J. Biochem. 122, 601-605 (1997); ibid. 126, 559-565 (1999)]. Through these studies, Phe139, Tyr149, Glu204, and Arg136 were estimated to be concerned with substrate recog...

journal_title：Journal of biochemistry

authors： Ito K,Inoue T,Kabashima T,Kanada N,Huang HS,Ma X,Azmi N,Azab E,Yoshimoto T

更新日期：2000-10-01 00:00:00

abstract：:A novel immortalized rheumatoid fibroblast-like synoviocyte (FLS) line, MH7A, was established by stably transfecting FLS cells with SV40 T antigen gene. MH7A cells expressed SV40-specific small t and large T antigens as well as an elevated level of p53 protein. They have already reached over 150 population doublings t...

journal_title：Journal of biochemistry

authors： Miyazawa K,Mori A,Okudaira H

更新日期：1998-12-01 00:00:00

abstract：:The pcd gene from Flavobacterium lutescens IFO3084 encoding Delta'-piperideine-6-carboxylate dehydrogenase (PCD) was cloned, sequenced, and expressed in Escherichia coli. The deduced amino acid sequence of PCD from F. lutescens IFO3084 showed strong similarity to that from Streptomyces clavuligerus. The molecular mass...

journal_title：Journal of biochemistry

authors： Fujii T,Narita T,Agematu H,Agata N,Isshiki K

更新日期：2000-12-01 00:00:00

abstract：:Upon CS2SO4 density-gradient equilibrium centrifugation, 60S subunits of rat liver ribosomes were dissociated to form three bands at the densities of 1.55, 1.40, and 1.30 g/ml. The bands at 1.55 and 1.30 g/ml were shown to contain exclusively RNA or proteins, respectively, whereas the band at 1.40 g/ml contained both ...

journal_title：Journal of biochemistry

authors： Isoda N,Tanaka T,Ishikawa K

更新日期：1981-08-01 00:00:00

abstract：:S-RNase is associated with the gametophytic self-incompatibility of flowering plants in Solanaceae and, on the basis of sequence homology, belongs to the RNase T2 family. To identify the active site residues in S-RNase, Nicotiana alata S6-RNase was studied by chemical modification. S6-RNase was inactivated with iodoac...

journal_title：Journal of biochemistry

authors： Ishimizu T,Miyagi M,Norioka S,Liu YH,Clarke AE,Sakiyama F

更新日期：1995-11-01 00:00:00

abstract：:Alanine-glyoxylate aminotransferase (AGT) 2 is a pyridoxal 5'-phosphate dependent, mitochondrial enzyme which, in the rat, is expressed at a high level in the kidney. The amino acid sequences of nine tryptic and seven CNBr peptides of the rat kidney AGT2 were determined. Three overlapping cDNAs encoding the AGT2 were ...

journal_title：Journal of biochemistry

authors： Lee IS,Muragaki Y,Ideguchi T,Hase T,Tsuji M,Ooshima A,Okuno E,Kido R

更新日期：1995-04-01 00:00:00

abstract：:To obtain direct evidence for the attachment of 5SrRNA-ribosomal L5 protein particles (5SRNP) and methionine-tRNA (tRNA(met)) to methionyl-tRNA synthetase (MetRS) in the macromolecular aminoacyl-tRNA synthetase (ARS) complex of rat liver, a MetRS-5SRNP-tRNA(met) complex was dissociated from the macromolecular ARS comp...

journal_title：Journal of biochemistry

authors： Ogata K,Ohno R,Morioka S,Terao K

更新日期：1996-11-01 00:00:00

abstract：:The effect of polyamines on F1-ATPase catalyzed reactions has been studied through the use of submitochondrial particles and F1-ATPase. ATP degradation catalyzed by submitochondrial particles and F1-ATPase was inhibited by spermine and spermidine. Spermine's inhibition was much greater than spermidine's effect. In con...

journal_title：Journal of biochemistry

authors： Igarashi K,Kashiwagi K,Kobayashi H,Ohnishi R,Kakegawa T,Nagasu A,Hirose S

更新日期：1989-08-01 00:00:00

abstract：:Heptic fibrosis/cirrhosis is a common hepatic disease characterized by the hyper-accumulation of connective tissue components, and hepatic necrosis. Chronic alcohol ingestion, viral infection, and metabolic disorders are contributing factors and there has been no effective treatment. Hepatocyte growth factor (HGF), or...

journal_title：Journal of biochemistry

authors： Matsuda Y,Matsumoto K,Ichida T,Nakamura T

更新日期：1995-09-01 00:00:00

abstract：:The Tetrahymena group I intron ribozyme folds into a complex three dimensional structure for performing the self-splicing reaction. Catalysis depends on its core structure comprising two helical domains, P4-P6 and P3-P7. The two domains are joined by three sets of conserved base-triple(s) and other tertiary interactio...

journal_title：Journal of biochemistry

authors： Ohki Y,Ikawa Y,Shiraishi H,Inoue T

更新日期：2002-11-01 00:00:00

abstract：:Streptolysin S (SLS) is a serum-extractable and oxygen-stable hemolysin produced by Group A Streptococcus. A SLS-deficient mutant in which transposon Tn 916 was inserted in a locus distinct from the sag gene cluster [Nizet et al. (2000) Infect. Immun. 68, 4245-4254] was obtained by filter mating of the transposon-harb...

journal_title：Journal of biochemistry

authors： Karaya K,Shimizu T,Taketo A

更新日期：2001-05-01 00:00:00

abstract：:Micromolar levels of free calcium ions added to the extracellular medium elicit secretion of serotonin from electropermeabilized bovine platelets in the presence of millimolar levels of Mg-ATP. Such Ca2(+)-dependent secretion of serotonin was almost completely impaired when the permeabilized platelets were preincubate...

journal_title：Journal of biochemistry

authors： Morimoto T,Oho C,Ueda M,Ogihara S,Takisawa H

更新日期：1990-08-01 00:00:00

abstract：:Rho-kinase is a main player in the regulation of cytoskeletal events and a promising drug target in the treatment of both vascular and neurological disorders. Here we report the crystal structure of the Rho-kinase catalytic domain in complex with the specific inhibitor Y-27632. Comparison with the structure of PKA bou...

journal_title：Journal of biochemistry

authors： Yamaguchi H,Miwa Y,Kasa M,Kitano K,Amano M,Kaibuchi K,Hakoshima T

更新日期：2006-09-01 00:00:00

abstract：:When mice were exposed to 100% oxygen at a pressure of 6 atm. absolute, the animals suffered from severe convulsions. The content of gamma-aminobutyric acid (GABA) in synaptosomes was lower in the exposed animals than in unexposed ones. The exposure to high pressure oxygen produced a considerable reduction in GABA for...

journal_title：Journal of biochemistry

authors： Hori S

更新日期：1982-02-01 00:00:00

abstract：:We obtained two kinds of vesicle preparations which were of different density from the same gastric mucosae of hogs stimulated with food before slaughter. Both kinds contained H+,K+-ATPase. The light vesicle preparation differed from the heavy vesicle preparation as follows: the KCl permeability across the membrane of...

journal_title：Journal of biochemistry

authors： Asano S,Iino T,Tabuchi Y,Takeguchi N

更新日期：1988-04-01 00:00:00

abstract：:Pork insulin was subjected to mercaptosuccinylation and then coupled to beta-D-galactosidase [EC 3.2.1.23] from Escherichia coli using N,N'-o-phenylenedimaleimide. The competitive binding of the conjugate and insulin to anti-insulin antibody was tested. Results showed that formation of an insulin-beta-D-galactosidase ...

journal_title：Journal of biochemistry

authors： Kato K,Hamaguchi Y,Fukui H,Ishikawa E

更新日期：1975-07-01 00:00:00

abstract：:Dimer formation of HIV-1 genomic RNA through its dimerization initiation site (DIS) is crucial to maintaining infectivity. Two types of dimers, the initially generated kissing-loop dimer and the subsequent product of the extended-duplex dimer, are formed in the stem-bulge-stem region with a loop including a self-compl...

journal_title：Journal of biochemistry

authors： Baba S,Takahashi K,Noguchi S,Takaku H,Koyanagi Y,Yamamoto N,Kawai G

更新日期：2005-11-01 00:00:00

abstract：:The nucleotide sequence at the intron-exon junction in the human beta-globin gene was analyzed by the quantification method (categorical discriminant analysis) proposed previously. Using the sample score of a 16-nucleotide sequence at a 3'-splice junction, we studied to what extent such a sequence contains the 3'-spli...

journal_title：Journal of biochemistry

authors： Iida Y

更新日期：1990-12-01 00:00:00

abstract：:beta-Hydroxyisovalerylshikonin (beta-HIVS), which was isolated from the plant, Lithospermium radix, inhibited the growth of various lines of cancer cells derived from human solid tumors at low concentrations between 10(-8) and 10(-6) M. When HL-60 cells were treated with 10(-6) M beta-HIVS for 3 h, characteristic feat...

journal_title：Journal of biochemistry

authors： Hashimoto S,Xu M,Masuda Y,Aiuchi T,Nakajo S,Cao J,Miyakoshi M,Ida Y,Nakaya K

更新日期：1999-01-01 00:00:00

abstract：:We have previously purified two D-3-hydroxyacyl-CoA dehydratase preparations from human liver. One preparation contained a 77-kDa polypeptide and smaller polypeptides, and the other was a homodimer of a 46-kDa polypeptide. Three different purified rat peroxisomal D-3-hydroxyacyl-CoA dehydratase preparations have been ...

journal_title：Journal of biochemistry

authors： Jiang LL,Miyazawa S,Hashimoto T

更新日期：1996-09-01 00:00:00

abstract：:A spectrofluorometric method for sensitive determination of metalloendopeptidase activity has been developed by using a bimane-peptide containing a tryptophan residue, i.e. 1,7-dioxo-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-alpha]pyrazol-3-yl-methyl- thiomethylcarbonyl-phenylalanyl-tryptophanyl-leucine (Bim-SCH2CO-Phe-Trp-L...

journal_title：Journal of biochemistry

authors： Kajiwara K,Kumazaki T,Sato E,Kanaoka Y,Ishii S

更新日期：1991-09-01 00:00:00