Affinity purification and characterization of serine hydroxymethyltransferases from rat liver.

abstract：:An NAD(P)H-dependent C-nitrosoreductase was purified 90 fold from porcine heart cytosol fraction by ammonium sulfate fractionation, gel filtration with Sephadex G-100, ion-exchange chromatography on DEAE-Sephadex A-50, and affinity chromatography on 5'-AMP-Sepharose. The enzyme had no aldehyde reductase activity and s...

journal_title：Journal of biochemistry

authors： Horie S,Ogura Y

更新日期：1980-10-01 00:00:00

abstract：:Cation specificity and effects of pH and temperature on membrane aggregation of porcine intestinal brush borders were studied in terms of the turbidity change of the membrane suspensions due to the addition of polyvalent cations. Monovalent cations (K+ and Na+) did not induce the membrane aggregation at 5 mM or below....

journal_title：Journal of biochemistry

authors： Ohyashiki T,Takeuchi M,Mohri T

更新日期：1984-03-01 00:00:00

abstract：:High mobility group (HMG) protein 1 contains two DNA binding motifs, called HMG1/2-boxes, linked with a linker region. The functional relationships between the two boxes and the mechanism of involvement of the linker region for effective binding of HMG1 were examined. The binding analyses of truncated HMG1 peptides wi...

journal_title：Journal of biochemistry

authors： Saito K,Kikuchi T,Shirakawa H,Yoshida M

更新日期：1999-02-01 00:00:00

abstract：:The effect of ryanodine on the permeation of glucose, choline, and Ca2+ through the Ca2+ channel in sarcoplasmic reticulum vesicles was studied by means of the light scattering and/or the tracer method. Ryanodine had the same effect on the permeabilities of choline and Ca2+; low concentrations of ryanodine (10 microM)...

journal_title：Journal of biochemistry

authors： Kasai M,Kawasaki T

更新日期：1993-03-01 00:00:00

abstract：:Galactocerebrosidase was purified about 22,600-fold using several hydrophobic column and gel filtration steps with a 4.8% recovery, from human lymphocytes. Its specific activity was 1.54 x 10(5) nmol/h/mg with tritium-labeled galactocerebroside as the substrate in the taurocholate system. The optimal pH for galactocer...

journal_title：Journal of biochemistry

authors： Sakai N,Inui K,Midorikawa M,Okuno Y,Ueda S,Iwamatsu A,Okada S

更新日期：1994-09-01 00:00:00

abstract：:The activity of biological molecules is often affected by their phosphorylation state. Regulatory phosphorylation operates as a binary switch and is usually controlled by counteracting kinases and phosphatases. However, phosphatidylinositol (PtdIns) has three phosphorylation sites on its inositol ring. The phosphoryla...

journal_title：Journal of biochemistry

authors： Miyazawa K

更新日期：2013-01-01 00:00:00

abstract：:The process of mitochondrial dysfunction in ischemic rat liver was studied. A close correlation was found between decrease in the mitochondrial adenine nucleotide content and deterioration of oxidative phosphorylation capacity. The level of total adenine nucleotides, which was 15--20 nmol/mg protein in mitochondria is...

journal_title：Journal of biochemistry

authors： Watanabe F,Kamiike W,Nishimura T,Hashimoto T,Tagawa K

更新日期：1983-08-01 00:00:00

abstract：:Human platelet membrane glycoproteins IIb (GPIIb) and IIIa (GPIIIa), which have been proposed to be subunits of a receptor for fibrinogen, were purified from Triton X-100-solubilized platelet membranes by affinity chromatography on a concanavalin A (Con A)-Sepharose column followed by preparative sodium dodecyl sulfat...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-11-01 00:00:00

abstract：:The region containing the RNA polymerase alpha subunit (RNAPalpha) gene (rpoA) and the ribosomal protein genes of a thermophilic eubacterial strain, Thermus thermophilus (Tt) HB8, was cloned from a genomic DNA library by Southern hybridization. The gene order in this region is rpl36-rps13-rps11-rps4-rpoA-rpl17, which ...

journal_title：Journal of biochemistry

authors： Wada T,Yamazaki T,Kuramitsu S,Kyogoku Y

更新日期：1999-01-01 00:00:00

abstract：:Site-directed mutagenesis studies of recombinant Trimeresurus flavoviridis venom gland phospholipase A2 (PLA2) showed that the Asn residue at position 67 takes part in recognition of the substrate 2-arachidonoyl sn-glycero-3-phosphocholine in both monomeric and micellar states. The amount of arachidonate released from...

journal_title：Journal of biochemistry

authors： Ogawa T,Shimohigashi Y,Ohno M

更新日期：1997-11-01 00:00:00

abstract：:We isolated the human arylhydrocarbon receptor (AhR) cDNA from a human lung cDNA library, by using mouse AhR cDNA as a labeled probe. The nucleotide sequence of cloned human AhR cDNA is identical to the previously reported human AhR sequence [Dolwick et al. (1993), Mol. Pharmacol. 44, 911-917] from cell line HepG2. Th...

journal_title：Journal of biochemistry

authors： Ema M,Matsushita N,Sogawa K,Ariyama T,Inazawa J,Nemoto T,Ota M,Oshimura M,Fujii-Kuriyama Y

更新日期：1994-10-01 00:00:00

abstract：:One mg protein/ml of sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle were solubilized with 50 mg/ml of octaethyleneglycol mono n-dodecyl ether (C12E8) in a solution containing 5 mM CaCl2, 0.1 M KCl, and 20% glycerol at pH 7.5. When 30 mg/ml of soybean lecithin was added to this mixture and t...

journal_title：Journal of biochemistry

authors： Andoh R,Yamamoto T

更新日期：1985-03-01 00:00:00

abstract：:CEL-III is a haemolytic lectin, which has two beta-trefoil domains (domains 1 and 2) and a beta-sheet-rich domain (domain 3). In domain 3 (residues 284-432), there is a hydrophobic region containing two alpha-helices (H8 and H9, residues 317-357) and a loop between them, in which alternate hydrophobic residues, especi...

journal_title：Journal of biochemistry

authors： Hisamatsu K,Tsuda N,Goda S,Hatakeyama T

更新日期：2008-01-01 00:00:00

abstract：:beta1,4-galactosyltransferase II (beta1,4GalT II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Previously, we have reported that beta1,4GalT II overexpression increased cisplatin-induced HeLa cell apoptosis. However, the mechanisms of its expression regula...

journal_title：Journal of biochemistry

authors： Zhou J,Wei Y,Liu D,Ge X,Zhou F,Jiang XY,Gu J

更新日期：2008-04-01 00:00:00

abstract：:Cathepsin E, an intracellular aspartic proteinase of the pepsin family, is composed of two homologous domains, each containing the catalytic Asp residue in a consensus DTG motif. Here we examine the significance of residues in the motifs of rat cathepsin E by substitution of Asp98, Asp283, and Thr284 with other residu...

journal_title：Journal of biochemistry

authors： Liu J,Tsukuba T,Okamoto K,Ohishi M,Yamamoto K

更新日期：2002-09-01 00:00:00

abstract：:We found functionally active thrombomodulin in human platelets (60 +/- 18 molecules per platelet). Protein C appeared not to be activated by thrombin with gel-filtered platelets. However, the activation of protein C by thrombin was accelerated by thrombin-stimulated and washed platelets. This cofactor activity of the ...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Hayashi T,Kosaka Y

更新日期：1988-10-01 00:00:00

abstract：:Recent studies by our group suggested that lysozyme has a high affinity for bacterial lipopolysaccharide (LPS) of both the smooth and rough forms, and inhibits various immunomodulatory activities of LPS. GLA60 is a synthetic monosaccharide analogue of bacterial lipid A well known as having most of the activities of li...

journal_title：Journal of biochemistry

authors： Tanida N,Ohno N,Adachi Y,Matsuura M,Nakano M,Kiso M,Hasegawa A,Yadomae T

更新日期：1992-11-01 00:00:00

abstract：:A cell-wall-associated proteinase from Lactobacillus helveticus CP790, grown in skim milk, was purified to homogeneity by DEAE ion exchange chromatography in the presence of EDTA. Its molecular weight was estimated to be 45,000 by SDS-PAGE and also by the recovery of proteinase activity only from this fraction of SDS-...

journal_title：Journal of biochemistry

authors： Yamamoto N,Akino A,Takano T

更新日期：1993-11-01 00:00:00

abstract：:The alpha-subunit of rod photoreceptor G protein transducin (G(t1)alpha) is heterogeneously modified at the N-terminus by a mixture of acyl groups, laurate (C12:0), myristate (C14:0), and two unsaturated fatty acids (C14:1 and C14:2). Although the N-fatty acylation of G(t1)alpha plays important roles in protein-protei...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Matsuda T,Matsuura Y,Haga T,Fukada Y

更新日期：2004-03-01 00:00:00

abstract：:Aplyronine A is a macrolide isolated from Aplysia kurodai. By monitoring fluorescent intensity of pyrenyl-actin, it was found that aplyronine A inhibited both the velocity and the degree of actin polymerization. Aplyronine A also quickly depolymerized F-actin. The kinetics of depolymerization suggest that aplyronine A...

journal_title：Journal of biochemistry

authors： Saito S,Watabe S,Ozaki H,Kigoshi H,Yamada K,Fusetani N,Karaki H

更新日期：1996-09-01 00:00:00

abstract：:Palmitoyl CoA inhibited EDTA-ATPase of heavy meromyosin (HMM) prepared from rabbit skeletal muscle. The concentration for half maximum inhibition of EDTA-ATPase was about 18 microM. Myristoyl CoA, the other long chain fatty acyl CoA, also inhibited EDTA-HMM ATPase, but CoA and short chain CoA thioesters, such as butyr...

journal_title：Journal of biochemistry

authors： Fujiwara A,Fujisaki H,Asai H,Yasumasu I

更新日期：1981-09-01 00:00:00

abstract：:EnvZ is a membrane-located protein kinase which modulates expression of the ompF and ompC genes through phosphotransfer signal transduction in Escherichia coli. Previously, we developed an in vitro method for analyzing the intact form of EnvZ in isolated cytoplasmic membranes, and demonstrated that this particular for...

journal_title：Journal of biochemistry

authors： Tokishita S,Kojima A,Mizuno T

更新日期：1992-06-01 00:00:00

abstract：:We have previously purified two D-3-hydroxyacyl-CoA dehydratase preparations from human liver. One preparation contained a 77-kDa polypeptide and smaller polypeptides, and the other was a homodimer of a 46-kDa polypeptide. Three different purified rat peroxisomal D-3-hydroxyacyl-CoA dehydratase preparations have been ...

journal_title：Journal of biochemistry

authors： Jiang LL,Miyazawa S,Hashimoto T

更新日期：1996-09-01 00:00:00

abstract：:A new sulfated polysaccharide was isolated from the culture supernatant of a strain of Arthrobacter sp. The polysaccharide purified with quaternary ammonium salts consists of D-galactose, D-glucose, sulfate, phosphorus, glucosamine, muramic acid, alanine, glutamic acid, glycine, and LL-diaminopimelic acid in a molar r...

journal_title：Journal of biochemistry

authors： Inoue K,Korenaga H,Kadoya S

更新日期：1982-12-01 00:00:00

abstract：:We developed a high-throughput Enzyme-linked immunosorbent assay (ELISA) for measuring α2,6-sialylated transferrin (Tf), based on inhibition of anti-Tf antibody binding to α2,6-sialylated Tf by a lectin, Sambucus sieboldiana Agglutinin (SSA). The inhibition was not observed with other glycoforms, such as periodate-tre...

journal_title：Journal of biochemistry

authors： Hoshi K,Kariya Y,Nara K,Ito H,Matsumoto K,Nagae M,Yamaguchi Y,Nakajima M,Miyajima M,Arai H,Kuno A,Narimatsu H,Shirotani K,Hashimoto Y

更新日期：2013-09-01 00:00:00

abstract：:Spleen cells from a CBF1 (BALB/c X C57BL/6) mouse immunized with rat tyrosine 3-monooxygenase were fused with NS-1 mouse myeloma cells. From 188 hybrid cells, 2 stable clones secreting anti-tyrosine 3-monooxygenase antibody were obtained. Antibody from one clone was coupled to CNBr-activated Sepharose 4B and the monoc...

journal_title：Journal of biochemistry

authors： Okuno S,Fujisawa H

更新日期：1985-01-01 00:00:00

abstract：:The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis usi...

journal_title：Journal of biochemistry

authors： Kubo T,Sasaki M,Nakamura J,Sasagawa H,Ohashi K,Takeuchi H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase that is exclusively expressed in cells of the gastrointestinal tracts, lymphoid tissues, urinary organs and red blood cells. However, the molecular mechanism by which CE is predominantly expressed in these cells remains unknown. Here, we report the identificatio...

journal_title：Journal of biochemistry

authors： Okamoto K,Okamoto Y,Kawakubo T,Iwata J,Yasuda Y,Tsukuba T,Yamamoto K

更新日期：2012-03-01 00:00:00

abstract：:The circadian change of gluconeogenesis in the liver in vivo in fed rats was studied. Gluconeogenesis in the liver in vivo was determined by measuring the rate of synthesis of 14C-labeled blood glucose derived from a 14C-labeled substrate, such as lactate, pyruvate or an amino acid injected into nephrectomized rats. G...

journal_title：Journal of biochemistry

authors： Kida K,Nishio T,Yokozawa T,Nagai K,Matsuda H,Nakagawa H

更新日期：1980-10-01 00:00:00

abstract：:The mobility of spin labels covalently bound to the Ca2+-transport ATPase (ATP phosphohydrolase [EC 3.y.1.3]) was studied by electron spin-resonance spectroscopy in purified ATPase and reconstituted vesicles. The purified ATPase of sarcoplasmic reticulum of rabbit skeletal muscle was covalently labeled with maleimide ...

journal_title：Journal of biochemistry

authors： Nakamura H,Martonosi AN

更新日期：1981-01-01 00:00:00