Abstract:
:The process of mitochondrial dysfunction in ischemic rat liver was studied. A close correlation was found between decrease in the mitochondrial adenine nucleotide content and deterioration of oxidative phosphorylation capacity. The level of total adenine nucleotides, which was 15--20 nmol/mg protein in mitochondria isolated from normal liver, fell to 1--2 nmol/mg protein with concomitant loss of oxidative phosphorylation capacity after anoxic incubation in vitro or in vivo for 120 min. However, neither the permeability barrier to adenine nucleotides nor matrix enzymes were affected under these conditions. The loss of adenine nucleotides was ascribed to degradation of AMP to adenosine and then leakage of the latter. Conventional procedures for maintenance of oxidative phosphorylation capacity of isolated mitochondria, preservation in the cold and addition of ATP or a respiratory substrate under aerobic conditions, were very effective in maintaining the intramitochondrial levels of adenine nucleotides. Of the three species of adenine nucleotides, only AMP was ineffective in maintaining mitochondrial function; mitochondria containing more than 5 nmol of ATP plus ADP/mg protein exhibited normal activity of oxidative phosphorylation, but with less than 2 nmol they showed no activity.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Watanabe F,Kamiike W,Nishimura T,Hashimoto T,Tagawa Kdoi
10.1093/oxfordjournals.jbchem.a134380subject
Has Abstractpub_date
1983-08-01 00:00:00pages
493-9issue
2eissn
0021-924Xissn
1756-2651journal_volume
94pub_type
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1977-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1982-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133823
更新日期:1982-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123669
更新日期:1991-12-01 00:00:00
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更新日期:1999-07-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:2015-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1986-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1985-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:2006-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132081
更新日期:1978-06-01 00:00:00
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