Abstract:
:Syk protein-tyrosine kinase (PTK) has been implicated in a variety of hematopoietic cell responses including immunoreceptor signaling. However, so far, there has been no evidence of the expression of Syk or Syk-related PTK in non-hematopoietic tissues. In this study, we have purified from blood cell-depleted rat liver a 72-kDa cytoplasmic PTK which shows cross-reactivity with anti-Syk antibody. Partial amino acid sequence analysis revealed that this 72-kDa PTK is identical to Syk. Immunohistochemical and RT-PCR analyses demonstrated that Syk is expressed in human hepatocytes and two rat liver-derived cell lines, JTC-27 and RLC-16. Furthermore, Syk is significantly tyrosine-phosphorylated in response to angiotensin II in JTC-27 cells, and angiotensin II-induced MAP kinase activation is blocked by the treatment of cells with a Syk-selective inhibitor, piceatannol. These results suggest that Syk plays an important role in signaling events of hepatocytes, such as signaling steps leading to MAP kinase activation by G-protein-coupled receptors. This is the first report of the expression of Syk in non-hematopoietic tissue.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Tsuchida S,Yanagi S,Inatome R,Ding J,Hermann P,Tsujimura T,Matsui N,Yamamura Hdoi
10.1093/oxfordjournals.jbchem.a022610keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
321-7issue
2eissn
0021-924Xissn
1756-2651journal_volume
127pub_type
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