Amino acid sequence and attachment sites of oligosaccharide units of porcine erythrocyte glycophorin.

abstract：:The lipA gene of Pseudomonas cepacia DSM3959 requires a downstream gene, limA, in oder to express lipase activity. The product of the lim gene, LimA, is a molecular chaperone required during the folding of lipase in oder for the lipase to adopt an active conformation. The lipase and LimA proteins have been shown to fo...

journal_title：Journal of biochemistry

authors： Hobson AH,Buckley CM,Jørgensen ST,Diderichsen B,McConnell DJ

更新日期：1995-09-01 00:00:00

abstract：:Droplet counter-current chromatography (DCC) a novel liquid-liquid partition technique, is a useful method for the separation of glycolipids. After a series of investigations on suitable experimental conditions for DDC, mouse brain chloroform-methanol-0.5% CaCl2-n-propanol (50:60:40:6) by the ascending method with 500...

journal_title：Journal of biochemistry

authors： Otsuka H,Yamakawa T

更新日期：1981-07-01 00:00:00

abstract：:1. 3'-Guanylyl-ethanol, 3'-guanylyl-propanol, and 3'-guanylyl-alpha-glycerol were synthesized by ribonuclease N1 [EC 3.1.4.8] using guanosine 2',3'-cyclic phosphate as a phosphate donor and various alcohols as phosphate acceptors. The yields of these phosphodiesters were 15%, 13.5%, 38.2%, respectively, with respect t...

journal_title：Journal of biochemistry

authors： Tamanoi F,Uchida T,Egami F,Oshima T

更新日期：1976-07-01 00:00:00

abstract：:The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the pr...

journal_title：Journal of biochemistry

authors： Shiwa Y,Yoshikawa H,Tanaka T,Ogura M

更新日期：2015-05-01 00:00:00

abstract：:Fluorescence titration curves of 2-[4'-iodoacetamido)anilino)naphthalene-6-sulfonic acid-labeled troponin (IAANS-labeled Tn) and troponin-1-anilinonaphthalene-8-sulfonic acid (Tn-ANS) complex indicated that the fluorescent moiety, IAANS or ANS, detects conformational change of troponin I (TnI) or Tn due to the Ca2+ bi...

journal_title：Journal of biochemistry

authors： Nishio T,Iio T

更新日期：1983-09-01 00:00:00

abstract：:We recently found that budding yeast Saccharomyces cerevisiae sigma1278b, but not genome project strain S288C, has a gene conferring resistance to L-azetidine-2-carboxylic acid (AZC), a toxic four-membered ring analogue of L-proline. Also, the gene, designated as MPR1, encodes a novel acetyltransferase that detoxifies...

journal_title：Journal of biochemistry

authors： Nomura M,Nakamori S,Takagi H

更新日期：2003-01-01 00:00:00

abstract：:Phospholipid composition of biological membranes differs between the cytoplasmic and exoplasmic leaflets. The type 4 P-type ATPases are phospholipid flippases that generate such membrane phospholipid asymmetry. Drs2p, a flippase in budding yeast, is involved in the endocytic recycling pathway. Drs2p is implicated in c...

journal_title：Journal of biochemistry

authors： Hanamatsu H,Fujimura-Kamada K,Yamamoto T,Furuta N,Tanaka K

更新日期：2014-01-01 00:00:00

abstract：:Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. Cytochrome P-450cam and related enzymes participate in the early oxidation steps of D-camphor degradation metabolism. We cloned from a HindIII DNA lib...

journal_title：Journal of biochemistry

authors： Koga H,Yamaguchi E,Matsunaga K,Aramaki H,Horiuchi T

更新日期：1989-11-01 00:00:00

abstract：:A method was developed to obtain heavy meromyosin (HMM) from the tryptic digest of skipjack tuna dorsal myosin. The tuna HMM thus obtained was shown to be homogeneous on gel filtration-gel electrophoresis, and on ultracentrifugation. The sedimentation constant (S20,w) was estimated to be 6.1S for tuna HMM. The ATPase ...

journal_title：Journal of biochemistry

authors： Kimura I,Arai K,Watanabe S

更新日期：1979-12-01 00:00:00

abstract：:Monoclonal antibody 5E5 recognized an intranuclear antigen of neurons in the rat. We isolated 5E5cDNA and determined its nucleotide and deduced amino acid sequences. The 5E5cDNA had an open reading frame of 825 amino acids and its amino acid sequence showed no significant homology to any protein or to any DNA binding ...

journal_title：Journal of biochemistry

authors： Suzuki E,Kojima N,Yoshimura K,Uyemura K,Obata K,Akagawa K

更新日期：1995-07-01 00:00:00

abstract：:The sensitivity and specificity of two influenza C virus assays, solid-phase and overlay assays, were investigated using naturally occurring 9-O-acetylated GD(3), rat serum glycoproteins containing 60% of N-acetyl-9-O-acetylneuraminic acid, and synthetically O-acetylated sialylated compounds. The sensitivity of the so...

journal_title：Journal of biochemistry

authors： Hubl U,Ishida H,Kiso M,Hasegawa A,Schauer R

更新日期：2000-06-01 00:00:00

abstract：:The interaction of Pi with sarcoplasmic reticulum (SR) isolated from rabbit skeletal muscle was studied using bromocresol purple (BCP) as a probe and a dual-wavelength spectrophotometer. Two kinds of absorption-intensity changes controlled by a low concentration of Ca2+ (greater than 10(-6)M) were observed after addit...

journal_title：Journal of biochemistry

authors： Nakamaru Y,Nomura K

更新日期：1977-02-01 00:00:00

abstract：:Subtilisin BPN' was chemically converted to thiolsubtilisin and the interaction of this modified enzyme with Streptomyces subtilisin inhibitor (SSI) was examined. SSI competitively inhibited the esterolytic activity of thiolsubtilisin toward p-nitrophenyl acetate with a K1 value of 1.3 X 10(-5) M at pH 7.5 Spectrophot...

journal_title：Journal of biochemistry

authors： Fujiwara K,Inouye K,Tonomura B,Murao S,Tsuru D

更新日期：1977-07-01 00:00:00

abstract：:A direct spectrophotometric suspension method for the estimation of immobilized ligands on a gel matrix was developed. The method uses 0.05% agarose solution to prepare a practically stable suspension of various agarose beads. The quantity of agarose beads in the suspension was estimated turbidometrically from the att...

journal_title：Journal of biochemistry

authors： Miyagawa A,Okuyama T

更新日期：1977-06-01 00:00:00

abstract：:A transition-state analog, gluconolactone, was found to partially quench the protein fluorescence of glucoamylase [EC 3.2.1.3] from Rhizopus niveus. The interaction between gluconolactone and the enzyme was studied statically and kinetically at pH 4.5 in terms of fluorescence change. The dissociation constant Kd of th...

journal_title：Journal of biochemistry

authors： Ohnishi M,Yamashita T,Hiromi K

更新日期：1977-01-01 00:00:00

abstract：:Resonance Raman (RR) spectra were obtained in H2O or D2O solution for the purple intermediates of D-amino acid oxidase (DAO) with isotopically labeled substrates, i.e., [1-13C]-, [2-13C]-, [3-13C]-, [15N]-, and [3,3,3-D3]alanine; [carboxyl-13C]- and [15N]proline. RR spectra were also measured for the intermediates of ...

journal_title：Journal of biochemistry

authors： Nishina Y,Shiga K,Miura R,Tojo H,Ohta M,Miyake Y,Yamano T,Watari H

更新日期：1983-12-01 00:00:00

abstract：:Inhibitory effects of various amino acid esters on the phagocytic activity of guinea pig peritoneal macrophages were studied with sensitized 51Cr-sheep erythrocytes (51Cr-EAb) as well as 125I-alpha-amylase complexed with homologous IgG2 antibody (Ag-Ab complex). The intracellular uptake of 51Cr-EAb was markedly inhibi...

journal_title：Journal of biochemistry

authors： Nihira S,Koyama J

更新日期：1983-08-01 00:00:00

abstract：:Cell metabolism can be considered as a process of serial construction and destruction of cellular components, both of which must be regulated accurately. In eukaryotic cells, a variety of cellular components are actively delivered into lysosomes/vacuoles, specialized compartments for hydrolysis of macromolecules. Such...

journal_title：Journal of biochemistry

authors： Fujiwara Y,Wada K,Kabuta T

更新日期：2017-02-01 00:00:00

abstract：:We investigated the significance of the plasma membrane lipid composition in exocytosis in an in vitro interaction system using an intact secretory granular fraction (SG) isolated from the rat parotid gland. When various liposomes (as a model of plasma membranes) were added to this assay system, rapid and transient am...

journal_title：Journal of biochemistry

authors： Mizuno-Kamiya M,Inokuchi H,Kameyama Y,Yashiro K,Shin SO,Fujita A

更新日期：1995-10-01 00:00:00

abstract：:The entire mitochondrial genome of Rana catesbeiana was cloned into a plasmid vector pBR322 at the unique BamHI site and the nucleotide sequences of the ND2 gene and of its flanking genes were determined. The ND2 gene was encoded by 1,033 base pairs and, as deduced from the nucleotide sequence, the ND2 product consist...

journal_title：Journal of biochemistry

authors： Fujii H,Shimada T,Goto Y,Okazaki T

更新日期：1988-03-01 00:00:00

abstract：:The denaturation of lysozyme and ribonuclease A by guanidine hydrochloride was followed in the presence and absence of glycerol and sorbitol by means of circular dichroism measurements at 25 degrees C. The protein-solvent interactions in the presence of these polyols were also studied by means of density measurements,...

journal_title：Journal of biochemistry

authors： Gekko K,Ito H

更新日期：1990-04-01 00:00:00

abstract：:Resonance Raman spectra of the soluble-domain of a membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F were recorded in different oxidation states. In the oxidized state, the Raman band due to the totally symmetric stretching mode of the iron-sulfur cluster was observed at 341 cm-1, which was attributed ...

journal_title：Journal of biochemistry

authors： Furuichi H,Ozaki Y,Niki K,Akutsu H

更新日期：1990-11-01 00:00:00

abstract：:Galactocerebrosidase was purified about 22,600-fold using several hydrophobic column and gel filtration steps with a 4.8% recovery, from human lymphocytes. Its specific activity was 1.54 x 10(5) nmol/h/mg with tritium-labeled galactocerebroside as the substrate in the taurocholate system. The optimal pH for galactocer...

journal_title：Journal of biochemistry

authors： Sakai N,Inui K,Midorikawa M,Okuno Y,Ueda S,Iwamatsu A,Okada S

更新日期：1994-09-01 00:00:00

abstract：:Caenorhabditis elegans HMG-5, which is encoded by F45E4.9, contains two high mobility group (HMG) box domains and shows sequence similarity with mammalian mitochondrial transcription factor A (TFAM). In this study, using soaking RNA interference, we found that knockdown of HMG-5 reduced the amount of mtDNA in P0 herma...

journal_title：Journal of biochemistry

authors： Sumitani M,Kasashima K,Matsugi J,Endo H

更新日期：2011-05-01 00:00:00

abstract：:Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ de...

journal_title：Journal of biochemistry

authors： Tanaka Y,Maruyama K,Mikawa T,Hotta Y

更新日期：1988-10-01 00:00:00

abstract：:Nitric oxide (NO) has diverse effects on immune responses and hepatic functions. In BNL CL.2 cells, the murine embryonic liver cells, inducible nitric oxide synthase (iNOS) mRNA expression appeared after 3 h of treatment with IFN-gamma and LPS. Interestingly, mRNA and protein expression of iNOS was down-regulated by s...

journal_title：Journal of biochemistry

authors： Lee BS,Kim YM,Kang HS,Kim HM,Pyun KH,Choi I

更新日期：2001-01-01 00:00:00

abstract：:Induction of cytosolic aspartate aminotransferase (cAspAT) was observed in rat liver on administration of a high-protein diet, or glucagon and during fasting. The enzyme activity in the liver of rats given 80% protein diet or glucagon injection during starvation increased to 2- to 2.4-fold that in the liver of rats ma...

journal_title：Journal of biochemistry

authors： Horio Y,Tanaka T,Taketoshi M,Uno T,Wada H

更新日期：1988-05-01 00:00:00

abstract：:Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...

journal_title：Journal of biochemistry

authors： Watanabe A,Yonemura I,Gonda K,Numata O

更新日期：2000-01-01 00:00:00

abstract：:LukF and Hlg2 of staphylococcal gamma-hemolysin assemble into hetero-oligomeric pores on human red blood cells (HRBC). Here, we demonstrate, using a single-molecule imaging technique, that a W177T/R198T mutant of LukF, which exhibits no binding activity toward phosphatidylcholine, could form intermediate oligomers wit...

journal_title：Journal of biochemistry

authors： Monma N,Nguyen VT,Kaneko J,Higuchi H,Kamio Y

更新日期：2004-10-01 00:00:00

abstract：:Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each protein in different c...

journal_title：Journal of biochemistry

authors： Khademi F,Mostafaie A

更新日期：2010-05-01 00:00:00