Analysis of proteases involved in phagocytic activity of macrophages through the use of various amino acid esters.

abstract：:Our previous study showed that the amino acid sequence of Tetrahymena histone H2B has a blocked N-terminal residue followed by 119 amino acid residues, X-Pro-Lys.... [J. Biochem. (1982) 91, 897-904]. Now, X has been found to be N-trimethylalanine by 1H nuclear magnetic resonance spectroscopy of the isolated X-Pro-Lys ...

journal_title：Journal of biochemistry

authors： Nomoto M,Kyogoku Y,Iwai K

更新日期：1982-11-01 00:00:00

abstract：:Serine dehydrogenase from Escherichia coli is a homotetrameric enzyme belonging to the short-chain dehydrogenase/reductase (SDR) family. This enzyme catalyses the NADP(+)-dependent oxidation of serine to 2-aminomalonate semialdehyde. The enzyme shows a stereospecificity for β-(3S)-hydroxy acid as a substrate; however,...

journal_title：Journal of biochemistry

authors： Yamazawa R,Nakajima Y,Mushiake K,Yoshimoto T,Ito K

更新日期：2011-06-01 00:00:00

abstract：:The present study demonstrates that the functional counter-receptors for E-selectin at the cell surface of Colo201 human colon cancer cells are localized in detergent-insoluble membrane microdomains (DIM). Following isolation of counter-receptors from whole cell lysates using E-selectin-coupled magnetic beads followed...

journal_title：Journal of biochemistry

authors： Suzuki C,Kojima N

更新日期：2007-07-01 00:00:00

abstract：:Cathepsin E (CE) is an intracellular aspartic proteinase that is exclusively expressed in cells of the gastrointestinal tracts, lymphoid tissues, urinary organs and red blood cells. However, the molecular mechanism by which CE is predominantly expressed in these cells remains unknown. Here, we report the identificatio...

journal_title：Journal of biochemistry

authors： Okamoto K,Okamoto Y,Kawakubo T,Iwata J,Yasuda Y,Tsukuba T,Yamamoto K

更新日期：2012-03-01 00:00:00

abstract：:A gene for a methionine aminopeptidase (MAP; EC 3.4.11.18), which catalyzes the removal of amino-terminal methionine from the growing peptide chain on the ribosome, has been cloned from the hyperthermophilic Archaeon, Pyrococcus furiosus, by a novel method effectively using its cosmid protein library, sequenced and ex...

journal_title：Journal of biochemistry

authors： Tsunasawa S,Izu Y,Miyagi M,Kato I

更新日期：1997-10-01 00:00:00

abstract：:The amino acid sequence of the glycophorin from porcine erythrocyte membrane has been determined by Edman degradation. Porcine glycophorin is a polypeptide chain of 133 amino acid residues and contains 12 oligosaccharide units attached to the amino-terminal side of the molecule. Ten oligosaccharides are linked O-glyco...

journal_title：Journal of biochemistry

authors： Honma K,Tomita M,Hamada A

更新日期：1980-12-01 00:00:00

abstract：:A rapid and simple method was developed for the purification of serine hydroxymethyltransferases [EC 2.1.2.1]. The procedure involved ammonium sulfate precipitation, DEAE-cellulose column chromatography and affinity chromatography on an L-adsorbent. Through this procedure the cytosolic enzyme (s-SHMT) was purified 1,6...

journal_title：Journal of biochemistry

authors： Masuda T,Sakamoto M,Nishizaki I,Hayashi H,Yamamoto M,Wada H

更新日期：1987-03-01 00:00:00

abstract：:Site-specific recognition and DNA-binding activity of p53 are crucial for its tumour suppressor function. Previous reports have shown that metal ions can affect the specific recognition and DNA-binding activity of p53DBD. Here we firstly report that magnesium ion can bind to the protein and influence its DNA-binding a...

journal_title：Journal of biochemistry

authors： Xue Y,Wang S,Feng X

更新日期：2009-07-01 00:00:00

abstract：:The effects of freezing on Alcaligenes sp. nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, EC 1.7.2.1] dissolved in sodium phosphate (pH 7.2) were investigated. The nitrite reductase was gradually activated with time in the frozen state, resulting in an increase in its activity of 2.5-4.5 times. The...

journal_title：Journal of biochemistry

authors： Masuko M,Iwasaki H,Sakurai T,Suzuki S,Nakahara A

更新日期：1985-11-01 00:00:00

abstract：:The conformation of the chondroitin-4-sulfate calcium salt was investigated by X-ray analysis. The following results were obtained. 1, The repeat length per disaccharide was 0.913 nm: 2, The molecular chain had three-fold screw symmetry: 3, The shape of the unit cell was a trigonal prism with dimensions a=b=1.28 nm, c...

journal_title：Journal of biochemistry

authors： Tanaka K

更新日期：1978-01-01 00:00:00

abstract：:2-Amino-4-methylphenol was converted to a brownish yellow material by the lysates of human erythrocytes or purified human hemoglobin. The reaction proceeded oxidatively, coupled with the oxidation of hemoglobin. The major component of the brownish yellow material produced by oxidative condensation of 2-amino-4-methylp...

journal_title：Journal of biochemistry

authors： Tomoda A,Arisawa M,Koshimura S

更新日期：1991-12-01 00:00:00

abstract：:We have recently developed an in vitro yeast reconstituted translation system, which is capable of synthesizing long polypeptides. Utilizing the system, we examined the role of eIF5A and its hypusine modification in translating polyproline sequence within long open reading frames. We found that polyproline motif inser...

journal_title：Journal of biochemistry

authors： Abe T,Nagai R,Shimazaki S,Kondo S,Nishimura S,Sakaguchi Y,Suzuki T,Imataka H,Tomita K,Takeuchi-Tomita N

更新日期：2020-05-01 00:00:00

abstract：:The circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of horse heart metmyoglobin and the following derivatives were measured in the Soret and near ultraviolet regions: metmyoglobin and its peroxide compound, and hydroxide, cyanide, azide, and fluoride derivatives. The heme-related CD bands in the ...

journal_title：Journal of biochemistry

authors： Kajiyoshi M,Anan FK

更新日期：1975-11-01 00:00:00

abstract：:Angiogenesis, a formation of neovessels, is regulated by the local balance between angiogenesis stimulators and inhibitors. A number of such endogenous regulators of angiogenesis have been found in the body. Recently, vasohibin-1 (VASH1) was isolated as a negative feedback regulator of angiogenesis produced by endothe...

journal_title：Journal of biochemistry

authors： Sato Y

更新日期：2013-01-01 00:00:00

abstract：:Large amounts of extra proteins, X (in the envelope fraction) and X' (in the cytoplasmic fraction) were detected by SDS-polyacrylamide gel electrophoresis when DNA of Escherichia coli was damaged. These two proteins had the same apparent molecular weight (appproximately 40,000) and were produced under identical condit...

journal_title：Journal of biochemistry

authors： Yoda K,Nagai K,Tamura G

更新日期：1977-05-01 00:00:00

abstract：:It is known that ABO blood group substances in human erythrocyte membranes are sphingoglycolipids, but recently several authors have reported that the glycoproteins of the erythrocyte membranes also have ABO blood group activities in addition to MN blood group activities and virus hemagglutination inhibitor activity. ...

journal_title：Journal of biochemistry

authors： Yamato K,Handa S,Yamakawa T

更新日期：1975-12-01 00:00:00

abstract：:Protein-tyrosine kinase p72syk exists abundantly in various hematopoietic cells and is activated by physiological or non-physiological agents. In this study we used vanadate, which is well known as a protein-tyrosine phosphatase inhibitor, to investigate the activity of p72syk and a downstream substrate for p72syk. Tr...

journal_title：Journal of biochemistry

authors： Nagai K,Inazu T,Yamamura H

更新日期：1994-11-01 00:00:00

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00

abstract：:This study presents the structural, kinetic and thermodynamic characterizations of previously unknown submicellar concentrations of SDS-induced molten globule (MGSDS) state of a highly negatively charged base-denatured ferricytochrome c (UB-state) at pH ∼12.8 (±0.2). The far-UV CD, near-UV CD, ANS-fluorescence data of...

journal_title：Journal of biochemistry

authors： Jain R,Sharma D,Kumar R,Kumar R

更新日期：2019-02-01 00:00:00

abstract：:Equilibrium gel permeation chromatography was employed to determine the ability of heparin to form complexes with thrombin and antithrombin III. In the eluate from a Sephacryl S-200 column, heparin caused a peak and then a trough in the fluorescence of 48 nM antithrombin III or 63 nM thrombin. The peak-heights with kn...

journal_title：Journal of biochemistry

authors： Oshima G,Uchiyama H,Nagasawa K

更新日期：1984-10-01 00:00:00

abstract：:Light harvesting (LH) bacteriochlorophyll (Bchl) protein complexes were isolated from chromatophores of Chromatium vinosum, by the combination of detergent solubilization, sodium dodecylsulfate (SDS) polyacrylamide gel electrophoresis (PAGE) and hydroxyapatite chromatography. Addition of the absorption spectra of thes...

journal_title：Journal of biochemistry

authors： Hayashi H,Morita S

更新日期：1980-11-01 00:00:00

abstract：:Heptic fibrosis/cirrhosis is a common hepatic disease characterized by the hyper-accumulation of connective tissue components, and hepatic necrosis. Chronic alcohol ingestion, viral infection, and metabolic disorders are contributing factors and there has been no effective treatment. Hepatocyte growth factor (HGF), or...

journal_title：Journal of biochemistry

authors： Matsuda Y,Matsumoto K,Ichida T,Nakamura T

更新日期：1995-09-01 00:00:00

abstract：:A partially purified fraction (Component I) which catalyzed the ATP-PPi exchange reaction dependent only on valine and glycine among the five constituent amino acids of gramicidin A was obtained from crude extracts of Bacillus brevis ATCC 8185, which produces both gramicidin A and tyrocidines. This fraction was found ...

journal_title：Journal of biochemistry

authors： Akashi K,Kubota K,Kurahashi K

更新日期：1977-01-01 00:00:00

abstract：:In the yeast Saccharomyces cerevisiae, the expression of phospholipid biosynthetic genes, including the INO1 gene (encoding inositol-1-phosphate synthase), is coordinately regulated by a cis-acting transcriptional element, UAS(INO) (inositol-sensitive upstream activating sequence). For this paper we studied the effect...

journal_title：Journal of biochemistry

authors： Kagiwada S,Zen R

更新日期：2003-04-01 00:00:00

abstract：:Crystals as large as 0.5 X 0.3 X 0.2 mm of purified arginine-transfer RNA from Escherichia coli have been prepared by a vapour diffusion method. X-ray diffraction photographs showed that the crystals gave reflections up to 3.7 A spacing. They have a trigonal space group P31 2 1 (OR P32 2 1) and cell-dimensions a=97.2,...

journal_title：Journal of biochemistry

authors： Morikawa K,Sakamaki T,Nishimura Y,Mitsui Y,Aoki K,Iitaka Y,Tsuboi M,Nishimura S

更新日期：1978-08-01 00:00:00

abstract：:Methylation and demethylation of the methyl-accepting chemotaxis protein (MCP) was studied in vitro. The in vitro MCP methylating system showed the following characteristics. 1. Multiple bands of methylated MCP were produced. 2. The reaction could be separated into two phases: the initial phase, in which the level of ...

journal_title：Journal of biochemistry

authors： Hayashi H,Minoshima S,Ohba M

更新日期：1982-08-01 00:00:00

abstract：:The Ca2+-sensitive ATPase activity of rabbit skeletal myofibrils disappeared completely after treatment with a solution containing CDTA, a strong divalent cation chelator, at a low ionic strength. A gel electrophoretic study revealed that all troponin C and about half of myosin light chain 2 were removed from the myof...

journal_title：Journal of biochemistry

authors： Morimoto S,Ohtsuki I

更新日期：1987-02-01 00:00:00

abstract：:Newly recognized mammalian secretory proteins such as preprolactin are translocated across the endoplasmic reticulum (ER) in a signal recognition particle (SRP)-dependent manner. Recent studies revealed that there are two recognition steps for signal peptides during this translocation. The first step is recognition by...

journal_title：Journal of biochemistry

authors： Hatsuzawa K,Tagaya M,Mizushima S

更新日期：1997-02-01 00:00:00

abstract：:Synthesis and degradation of glycerophospholipids in HL-60 cells and retinoic acid (RA)-treated HL-60 cells were examined. The synthesis of each subclass of ethanolamine-containing glycerophospholipids was extremely suppressed in RA-treated HL-60 cells, while that of other glycerophospholipids was not seriously affect...

journal_title：Journal of biochemistry

authors： Naito M,Kudo I,Nakagawa Y,Waku K,Nojiri H,Saito M,Inoue K

更新日期：1991-10-01 00:00:00

abstract：:We have recently shown that furin, a mammalian homologue of the yeast precursor-processing endoprotease Kex2, is involved in precursor cleavage at sites marked by the Arg-X-Lys/Arg-Arg motif within the constitutive secretory pathway. In this study, we analyzed molecular and enzymatic properties of furin expressed in C...

journal_title：Journal of biochemistry

authors： Hatsuzawa K,Murakami K,Nakayama K

更新日期：1992-03-01 00:00:00