Abstract:
:Methylation and demethylation of the methyl-accepting chemotaxis protein (MCP) was studied in vitro. The in vitro MCP methylating system showed the following characteristics. 1. Multiple bands of methylated MCP were produced. 2. The reaction could be separated into two phases: the initial phase, in which the level of methylation increased as a result of multiple methylation; and the stationary phase, in which methylation and demethylation took place at the same velocity without apparent change in the level of methylation. 3. Pulse-chase analysis of the reaction showed that MCP was demethylated preferentially at the relatively highly methylated state. 4. The behavior of MCP in this system was similar to that of MCP stimulated in vivo with attractant. Based on the above results, an autonomous control mechanism of the level of MCP methylation is discussed.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Hayashi H,Minoshima S,Ohba Mdoi
10.1093/oxfordjournals.jbchem.a133945subject
Has Abstractpub_date
1982-08-01 00:00:00pages
391-7issue
2eissn
0021-924Xissn
1756-2651journal_volume
92pub_type
杂志文章abstract::A cDNA for mouse prolyl endopeptidase (PEP) was cloned and its nucleotide sequence determined. The overall amino acid sequence identity between mouse and other mammalian PEPs was about 96%. A specific inhibitor of PEP, N-benzyloxycarbonyl-thioprolyl-thioprolinal- dimethylacetal (ZTTA), inhibited DNA synthesis by Swiss...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021970
更新日期:1998-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131304
更新日期:1976-09-01 00:00:00
abstract::Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021439
更新日期:1996-09-01 00:00:00
abstract::Glucuronolactone reductase [EC 1.1.1.20] from rat kidney was purified over 300-fold by ammonium sulfate fractionation, chromatography on DEAE-cellulose and hydroxylapatite columns, and preparative isoelectric focusing. The substrate specificity of the enzyme in the reduction reaction was broad, and hexuronic acid was ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134588
更新日期:1984-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvh106
更新日期:2004-08-01 00:00:00
abstract::A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134395
更新日期:1983-08-01 00:00:00
abstract::A 45-60 kDa Gal/GalNAc-specific macrophage lectin was found to participate in the interaction between tumor cells and tumoricidal macrophages activated by an antitumor streptococcal preparation, OK-432, and in the tumoricidal activity of the activated macrophages. The binding between OK-432-elicited activated macropha...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122763
更新日期:1989-06-01 00:00:00
abstract::3-Isopropylmalate dehydrogenase (IPMDH) is a dimeric enzyme with a strongly hydrophobic core that is composed of residues from four alpha-helices. We replaced Glu253, which is found in the hydrophobic core and is part of the subunit interface of the Bacillus subtilis (Bs) IPMDH, with several other amino acids to probe...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvm082
更新日期:2007-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124977
更新日期:1995-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132190
更新日期:1978-10-01 00:00:00
abstract::Acid ceramidase (ACDase) metabolizes ceramide to sphingosine, leading to sphingosine 1-phosphate production. Reportedly, ACDase has been upregulated in prostate cancer. However, its regulatory mechanism remains unclear. LNCaP (androgen-sensitive prostate cancer cell line) but not PC3 and DU-145, (androgen-unresponsive...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvv039
更新日期:2015-10-01 00:00:00
abstract::Calpastatin, an endogenous inhibitor protein specifically acting on calpain [EC 3.4.22.17; Ca2+-dependent cysteine proteinase], was purified to apparent homogeneity from the cytosol fraction of human erythrocytes. The yield was 0.38 mg from 400 ml of blood. The purification procedures included DEAE-cellulose and Ultro...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134134
更新日期:1982-12-01 00:00:00
abstract::20S proteasomes from higher eukaryotes have immunological functions rather than those from archibacteria or yeast. To clarify the mechanism of the sorting and production of antigen-presenting peptides, it is important and worthwhile to determine the structure of mammalian proteasomes using a third generation synchrotr...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022709
更新日期:2000-06-01 00:00:00
abstract::A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvj098
更新日期:2006-05-01 00:00:00
abstract::We describe cross-linking conditions for myosin subfragment-1 and actin with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, with which the compositions of cross-linked species can be altered. The specific Mg-ATPase activity of cross-linked subfragment-1 in these preparations was almost the same in spite of the diffe...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124254
更新日期:1993-12-01 00:00:00
abstract::Glutamine synthetase from a Gram-positive acid-fast bacterium, Mycobacterium smegmatis, was purified to homogeneity from cells grown with glycerol-bouillon medium. Electron micrographs of the enzyme revealed a dodecameric arrangement of its subunits in two superimposed hexagonal rings, similar to the structure of glut...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134768
更新日期:1984-06-01 00:00:00
abstract::It has been demonstrated that the GM2 ganglioside cannot be cleaved by exo-beta-N-acetylhexosaminidases isolated from molds or bacterial sources. Here, a novel GM2 ganglioside-degrading enzyme was found in cells of Nocardia sp. This enzyme releases free fatty acids from the GM2 ganglioside. The chemical structure of t...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122212
更新日期:1988-01-01 00:00:00
abstract::The accumulation of unfolded proteins in the endoplasmic reticulum (ER) under ER stress conditions activates a series of homoeostatic responses collectively termed the unfolded protein response (UPR). The UPR is unique in which the molecular mechanisms it uses to transmit signals from the ER lumen to the nucleus are c...
journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
doi:10.1093/jb/mvp166
更新日期:2009-12-01 00:00:00
abstract::The stimulatory effects of glucose and lactate on protein synthesis by round spermatids (steps 1-8) were further studied. When the cells were incubated with lactate, the response of protein synthesis in round spermatids was closely related to the intracellular level of ATP. The ATP level in spermatids increased to 3.1...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133581
更新日期:1981-10-01 00:00:00
abstract::The epitopes of about 100 monoclonal antibodies against human type II DNA topoisomerase were mapped along the enzyme molecules. Although they were randomly and independently established, epitope sites were unevenly distributed the toward N-terminal or C-terminal region. We suggest that the central catalytic domain is ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003237
更新日期:2002-09-01 00:00:00
abstract::The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate wa...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022732
更新日期:2000-07-01 00:00:00
abstract::In this report, we describe a laser-latex combination system that enables membrane-impermeable molecules to penetrate cell membranes. Laser light (Q-switched Nd:YAG laser, 532.5 nm) was used to irradiate a mixture of commercial latex particles (blue dyed, 1 micro m in diameter) and mouse fibrosarcoma (Meth-A) cells. A...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvg132
更新日期:2003-08-01 00:00:00
abstract::The terminal bases of the viral ribonucleic acid from the Caulobacter ribonucleic acid phage phiCp2 were examined. The viral ribonucleic acid contained guanosine triphosphate (pppG) at the 5'-terminus and adenosine (AOH) at the 3'-terminus. ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132112
更新日期:1978-07-01 00:00:00
abstract::Pollen proteins from several grass species have been identified and characterized as causative allergens in grass pollinosis. In contrast, allergenic potential of pollen proteins from rice, which belongs to the same Poaceae family, has not well been investigated, despite that a few clinical cases have been reported on...
journal_title:Journal of biochemistry
pub_type: 临床试验,杂志文章
doi:10.1093/jb/mvt044
更新日期:2013-08-01 00:00:00
abstract::The gene coding for a Trichosanthes trypsin inhibitor analog (Ala-6-TTI) in which methionine at position 6 was replaced by alanine was synthesized chemically. The synthetic gene was cloned into plasmid pWR590-1 and expressed in Escherichia coli as a fusion protein composed of beta-galactosidase fragment of 590 amino a...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123863
更新日期:1992-07-01 00:00:00
abstract::Ornithine transcarbamylase [EC 2.1.3.3] has been purified from chick kidney to homogeneity. The molecular weight is 110,000 as determined by gel filtration. Sodium dodecylsulfate polyacrylamide gel electrophoresis of the enzyme showed that the enzyme exists as a trimer of identical subunits of 36,000 daltons like othe...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134476
更新日期:1983-10-01 00:00:00
abstract::EnvZ is a membrane-located protein kinase which modulates expression of the ompF and ompC genes through phosphotransfer signal transduction in Escherichia coli. Previously, we developed an in vitro method for analyzing the intact form of EnvZ in isolated cytoplasmic membranes, and demonstrated that this particular for...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123823
更新日期:1992-06-01 00:00:00
abstract::To understand the mechanism of activation of a receptor by its agonist, the excitation and relaxation processes of the vibrational states of the receptor should be examined. As a first approach to this problem, we calculated the normal vibrational modes of agonists (glutamate and kainate) and an antagonist (6-cyano-7-...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a002931
更新日期:2001-06-01 00:00:00
abstract::In a previous report (J. Biochem. 89, 1333-1335, 1981) we showed that 30 degrees C-treatment (pCa less than 6 and 2 mM MgCl2), like EDTA-treatment, caused a reversible removal of regulatory light-chains from Akazara adductor myosin. Utilizing the heat-treatment, we now show (a) that not half but the total removal of r...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134216
更新日期:1983-02-01 00:00:00
abstract::The nucleotide sequence at the intron-exon junction in the human beta-globin gene was analyzed by the quantification method (categorical discriminant analysis) proposed previously. Using the sample score of a 16-nucleotide sequence at a 3'-splice junction, we studied to what extent such a sequence contains the 3'-spli...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1990-12-01 00:00:00