Stimulation of protein synthesis in round spermatids from rat testes by lactate. II. Role of adenosine triphosphate (ATP).

abstract：:Physicochemical and biological properties of recombinant human erythropoietin (rhEPO) were compared with human urinary erythropoietin (uEPO). uEPO and rhEPO were purified to apparent homogeneity from the urine of patients with aplastic anemia and from the conditioned medium of Chinese hamster ovary (CHO) cells transfe...

journal_title：Journal of biochemistry

authors： Imai N,Kawamura A,Higuchi M,Oh-eda M,Orita T,Kawaguchi T,Ochi N

更新日期：1990-03-01 00:00:00

abstract：:A chitinase was purified from the culture filtrate of Streptomyces erythraeus (SE). The enzyme (SE chitinase) has a molecular weight of 30,000 and pI 3.7, and shows optimal activity at pH 5.0 with an optimal ionic strength of less than 0.2 M NaCl. SE chitinase could hydrolyze chitin and its derivatives, but could not ...

journal_title：Journal of biochemistry

authors： Hara S,Yamamura Y,Fujii Y,Mega T,Ikenaka T

更新日期：1989-03-01 00:00:00

abstract：:The rate of slow Li+ influx and the fraction of active form of acetylcholine receptor (AChR) of Electrophorus electricus membrane vesicles at equilibrium between the active and desensitized forms of the receptor were measured in the presence of various concentrations of phenyltrimethylammonium (PTA) and nereistoxin (N...

journal_title：Journal of biochemistry

authors： Aoshima H,Hori K,Yamamoto A

更新日期：1987-02-01 00:00:00

abstract：:A large COOH-terminal domain of Serratia marcescens serine protease (SSP) is essentially required for the excretion of the mature protease region through the outer membrane in Escherichia coli. For investigation of the possibility of transporting foreign periplasmic proteins by utilizing the function of the COOH-termi...

journal_title：Journal of biochemistry

authors： Shimada K,Ohnishi Y,Horinouchi S,Beppu T

更新日期：1994-08-01 00:00:00

abstract：:We studied the roles of cofilin, an actin-binding phosphoprotein, in superoxide production of neutrophil-like HL-60 cells triggered by opsonized zymosan (OZ). OZ caused dephosphorylation of cofilin as well as a transient increase of F-actin. Both reactions were complete within 30 s. Okadaic acid (OA) magnified the OZ-...

journal_title：Journal of biochemistry

authors： Nagaishi K,Adachi R,Kawanishi T,Yamaguchi T,Kasahara T,Hayakawa T,Suzuki K

更新日期：1999-05-01 00:00:00

abstract：:Immunoblotting showed that a monoclonal antibody, 3A10, binds to a series of rat brain-specific antigens with molecular masses of 150-, 120-, 118-, 106-, 104-, 79-, and 77-kDa. The expression of 3A10 antigens is dependent on the developmental stage of the brain; only the 106-kDa antigen is detected during embryonic st...

journal_title：Journal of biochemistry

authors： Harada A,Takeuchi K,Dohmae N,Takio K,Uenaka T,Aoki J,Inoue K,Umeda M

更新日期：1999-03-01 00:00:00

abstract：:The lipA gene of Pseudomonas cepacia DSM3959 requires a downstream gene, limA, in oder to express lipase activity. The product of the lim gene, LimA, is a molecular chaperone required during the folding of lipase in oder for the lipase to adopt an active conformation. The lipase and LimA proteins have been shown to fo...

journal_title：Journal of biochemistry

authors： Hobson AH,Buckley CM,Jørgensen ST,Diderichsen B,McConnell DJ

更新日期：1995-09-01 00:00:00

abstract：:The intracellular distribution of 10Sa RNA in Escherichia coli was investigated in cell extracts. Northern hybridization revealed that a large fraction of 10Sa RNA cosediments with 70S ribosomes. When 70S ribosomes were dissociated into 50S and 30S subunits in the presence of low levels of Mg2+ ions, almost all of the...

journal_title：Journal of biochemistry

authors： Komine Y,Kitabatake M,Inokuchi H

更新日期：1996-03-01 00:00:00

abstract：:Separation of 13-hydroperoxylinoleic acid or 13-hydroperoxylinolenic acid from linoleic acid or linolenic acid, respectively, was carried out easily and quickly by high performance liquid chromatography on porous polymer gel (TSK-Gel LS-140) using n-hexane/ethanol as an eluent. An eluent containing a large amount of n...

journal_title：Journal of biochemistry

authors： Aoshima H

更新日期：1977-08-01 00:00:00

abstract：:Phospholipase A2 of rat liver mitochondria was purified approximately 1,400-fold by extraction with KCl, and chromatographies on a Sephadex G-75 column and a diacyl-glycerophosphocholine-Sepharose affinity column. The purified enzyme was very labile when incubated either at 37 degrees C or 0 degrees C, and lost its ac...

journal_title：Journal of biochemistry

authors： Natori Y,Karasawa K,Arai H,Tamori-Natori Y,Nojima S

更新日期：1983-02-01 00:00:00

abstract：:To examine whether serine proteases of rat liver chromatin are also involved in the degradation of newly synthesized and unbound ribosomal proteins and histones, like the nuclear thiol protease which we reported previously (Tsurugi, K. & Ogata, K. (1979) Eur. J. Biochem. 101, 205-213), in vivo experiments were carried...

journal_title：Journal of biochemistry

authors： Tsurugi K,Oyanagi M,Ogata K

更新日期：1983-05-01 00:00:00

abstract：:"DNase binding protein" of 43K daltons as determined by SDS-polyacrylamide gel electrophoresis, was purified from the 0.1 M KCl-soluble (non-structural) fraction of chicken skeletal muscle. The protein was distinct from actin in amino acid composition and physicochemical properties. "DNase binding protein" was also is...

journal_title：Journal of biochemistry

authors： Kohama K,Holtzer H

更新日期：1981-02-01 00:00:00

abstract：:A soluble enzyme preparation (20,000 X g supernatant fraction), prepared from the mycelia of wild-type Neurospora crassa, was capable of transferring [14C]glucose from UDP-[14C]glucose into both trichloroacetic acid (TCA)-soluble and TCA-insoluble macromolecule products in the absence of added primer. These reactions ...

journal_title：Journal of biochemistry

authors： Takahara H,Matsuda K

更新日期：1977-06-01 00:00:00

abstract：:Enterococcus hirae V-ATPase, in contrast to most V-type ATPases, is resistant to N-ethylmaleimide (NEM). Alignment of the amino acid sequences of NtpA suggests that the NEM-sensitive Cys of V-type ATPases is replaced by Ala in E. hirae V-ATPase. Consistent with this prediction, the V-ATPase became sensitive upon subst...

journal_title：Journal of biochemistry

authors： Murata T,Yoshikawa Y,Hosaka T,Takase K,Kakinuma Y,Yamato I,Kikuchi T

更新日期：2002-11-01 00:00:00

abstract：:Ornithine transcarbamylase [EC 2.1.3.3] has been purified from chick kidney to homogeneity. The molecular weight is 110,000 as determined by gel filtration. Sodium dodecylsulfate polyacrylamide gel electrophoresis of the enzyme showed that the enzyme exists as a trimer of identical subunits of 36,000 daltons like othe...

journal_title：Journal of biochemistry

authors： Tsuji S

更新日期：1983-10-01 00:00:00

abstract：:Human HsRad51 protein assembles on a DNA molecule through cooperative binding and forms a long filament for homologous recombination. We have characterized the self-assembly of HsRad51 by measuring the fluorescence resonance energy transfer from the fluorescein-labeled protein to the rhodamine-labeled protein. Self-as...

journal_title：Journal of biochemistry

authors： Yoshioka K,Yumoto-Yoshioka Y,Fleury F,Takahashi M

更新日期：2003-05-01 00:00:00

abstract：:Site-directed mutagenesis studies of recombinant Trimeresurus flavoviridis venom gland phospholipase A2 (PLA2) showed that the Asn residue at position 67 takes part in recognition of the substrate 2-arachidonoyl sn-glycero-3-phosphocholine in both monomeric and micellar states. The amount of arachidonate released from...

journal_title：Journal of biochemistry

authors： Ogawa T,Shimohigashi Y,Ohno M

更新日期：1997-11-01 00:00:00

abstract：:We examined the effect of porcine brain Ca2+-dependent regulator (CDR) protein on microtubule (MT) assembly from microtubular proteins isolated from porcine brain by temperature-dependent cycles of assembly-disassembly. CDR exhibited a potent inhibitory effect on MT assembly in the presence of Ca2+, whereas it had lit...

journal_title：Journal of biochemistry

authors： Nishida E,Kumagai H,Ohtsuki I,Sakai H

更新日期：1979-05-01 00:00:00

abstract：:The activity of ornithine decarboxylase (ODC) in rabbit costal chondrocytes in culture markedly increased after addition of parathyroid hormone (PTH), reaching a maximum 4 to 5 h after PTH addition. The effect of PTH was dose-dependent both in the presence and absence of fetal calf serum. Addition of cycloheximide or ...

journal_title：Journal of biochemistry

authors： Takigawa M,Watanabe R,Ishida H,Asada A,Suzuki F

更新日期：1979-01-01 00:00:00

abstract：:We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...

journal_title：Journal of biochemistry

authors： Takeuchi M,Kamata K,Yoshida M,Kameda Y,Matsui K

更新日期：1990-07-01 00:00:00

abstract：:The conformation and stabilities of the CL fragment isolated from a type lambda Bence Jones protein and the fragment in which the intrachain disulfide bond had been reduced were studied by measuring CD, fluorescence, and ultraviolet absorption. The results indicated that no great conformational change occurs on reduct...

journal_title：Journal of biochemistry

authors： Goto Y,Hamaguchi K

更新日期：1979-11-01 00:00:00

abstract：:Oxidative activity of 9-anthraldehyde (9-AA) to 9-anthracenecarboxylic acid in monkey liver was mainly located in microsomes. The reaction required NADPH as an essential cofactor and was significantly inhibited by SKF 525-A, metyrapone, disulfiram, and CO, potent inhibitors of microsomal aldehyde oxygenase (MALDO). Tw...

journal_title：Journal of biochemistry

authors： Matsunaga T,Iwawaki Y,Watanabe K,Yamamoto I,Kageyama T,Yoshimura H

更新日期：1996-04-01 00:00:00

abstract：:To study how collagen synthesis is regulated in developing chick embryonic skin, hydroxyproline synthesis, incorporation of proline, and translational activity and content of collagen mRNA in 12-, 15-, and 18-day skins were determined and compared with each other. Hydroxyproline synthesis in the 18-day skins was marke...

journal_title：Journal of biochemistry

authors： Ohyama K,Tokimitsu I,Tajima S

更新日期：1991-08-01 00:00:00

abstract：:Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...

journal_title：Journal of biochemistry

authors： Yamauchi T,Moroishi T

更新日期：2020-11-23 00:00:00

abstract：:We obtained two kinds of vesicle preparations which were of different density from the same gastric mucosae of hogs stimulated with food before slaughter. Both kinds contained H+,K+-ATPase. The light vesicle preparation differed from the heavy vesicle preparation as follows: the KCl permeability across the membrane of...

journal_title：Journal of biochemistry

authors： Asano S,Iino T,Tabuchi Y,Takeguchi N

更新日期：1988-04-01 00:00:00

abstract：:This study aims to study the effects of intra-nuclear lncRNA MEG3 on the progression of prostate cancer and the underlying mechanisms. Expressions of relative molecules were detected by Quantitative real time PCR (qRT-PCR) and western blot. Chromatin immunoprecipitation and RNA immunoprecipitation (RIP) assays were us...

journal_title：Journal of biochemistry

authors： Zhou Y,Yang H,Xia W,Cui L,Xu R,Lu H,Xue D,Tian Z,Ding T,Cao Y,Shi Q,He X

更新日期：2020-03-01 00:00:00

abstract：:Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...

journal_title：Journal of biochemistry

authors： Watanabe A,Yonemura I,Gonda K,Numata O

更新日期：2000-01-01 00:00:00

abstract：:The circular dichroism (CD) and magnetic circular dichroism (MCD) spectra of horse heart metmyoglobin and the following derivatives were measured in the Soret and near ultraviolet regions: metmyoglobin and its peroxide compound, and hydroxide, cyanide, azide, and fluoride derivatives. The heme-related CD bands in the ...

journal_title：Journal of biochemistry

authors： Kajiyoshi M,Anan FK

更新日期：1975-11-01 00:00:00

abstract：:The absorption of bile alcohols by rat small intestine was studied using in vivo and in vitro preparations. In bile duct cannulated rats, intraduodenal administered bile alcohols were certainly absorbed and excreted in the bile, although the absorption was not so efficient as for bile acids. The absorption during a 24...

journal_title：Journal of biochemistry

authors： Hoshita T,Harada N,Morita I,Kihira K

更新日期：1981-11-01 00:00:00

abstract：:Previously, we showed that a polypeptide isolated from fetal bovine cartilage stimulates proteoglycan synthesis, RNA synthesis, and DNA synthesis in cultured rabbit costal chondrocytes. In the present study, the effects of the cartilage-derived factor (CDF) were compared with those of multiplication-stimulating activi...

journal_title：Journal of biochemistry

authors： Kato Y,Nomura Y,Tsuji M,Ohmae H,Nakazawa T,Suzuki F

更新日期：1981-11-01 00:00:00