Abstract:
:The lipA gene of Pseudomonas cepacia DSM3959 requires a downstream gene, limA, in oder to express lipase activity. The product of the lim gene, LimA, is a molecular chaperone required during the folding of lipase in oder for the lipase to adopt an active conformation. The lipase and LimA proteins have been shown to form a complex precipitable with either an anti-lipase or anti-LimA antibody. LimA has been shown to form a 1:1 complex with with prelipase and lipase isolated from "natural" P. cepacia system. The mature lipase (lacking its signal peptide) has been expressed in the presence and absence of LimA in Escherichia coli. LimA can activate mature lipase during a urea denaturation-renaturation experiment, indicating that the signal peptide is not required for the lipase to be activated by LimA. The effects of various reagents on the renaturation of lipase from 8 M urea have been examined. We propose a mechanism for the function of the LimA chaperone during the production of active extracellular lipase.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Hobson AH,Buckley CM,Jørgensen ST,Diderichsen B,McConnell DJdoi
10.1093/oxfordjournals.jbchem.a124948subject
Has Abstractpub_date
1995-09-01 00:00:00pages
575-81issue
3eissn
0021-924Xissn
1756-2651journal_volume
118pub_type
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更新日期:1989-08-01 00:00:00
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pub_type: 杂志文章,评审
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journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
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journal_title:Journal of biochemistry
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更新日期:1981-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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doi:10.1093/oxfordjournals.jbchem.a124485
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