Abstract:
:We have been interested in partially folded proteins with marginal stability and activity, because they have a potential to be mature proteins by artificial evolution. A module is defined as a contiguous peptide chain forming a compact region in a globular protein. Modules may be used as building blocks to create partially folded proteins. Barnase, a ribonuclease consisting of 110 amino acids, has been divided into six modules (M1-M6), four peptide fragments, M12 (1-52), M123 (1-73), M1234 (1-88) and M12345 (1-98), have been constructed by progressive elongation of the modules from the N-terminus. Only M12345 (1-98) had a partially folded conformation, but it lacked detectable RNase activity. A mixture of M12345 (1-98) with M56 (89-110) showed weak but distinct RNase activity. Unfolded M12345 (1-96) was constructed by removal of two residues from the C-terminus of M12345 (1-98). The mixture of M12345 (1-96) with M56 (89-110) also showed RNase activity. Further, the interaction endowed M12345 (1-96) with conformational stability. We propose that N- and C-terminal fragments obtained by successive elongation of modules would interact to be a complex with marginal stability and activity, which would be used for creating a mature complex by artificial evolution.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Tsuji T,Nagata T,Yanagawa Hdoi
10.1093/jb/mvn099subject
Has Abstractpub_date
2008-10-01 00:00:00pages
513-21issue
4eissn
0021-924Xissn
1756-2651pii
mvn099journal_volume
144pub_type
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更新日期:2004-01-01 00:00:00
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更新日期:1976-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131814
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132683
更新日期:1979-12-01 00:00:00
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更新日期:1999-09-01 00:00:00
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更新日期:2010-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1980-12-01 00:00:00
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更新日期:1981-02-01 00:00:00
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