Abstract:
:As a first step to catalogue mRNAs present in mouse embryonal carcinoma F9 cells, 879 clones corresponding to low-abundance mRNAs were selected from among 2,896 randomly picked up clones of undifferentiated F9 cDNA libraries, using DNA probes complementary to poly(A)+RNAs prepared from undifferentiated F9 cells and to ones prepared from mouse fibroblast L cells. Five-hundred and eighty-two of the 879 clones were partially sequenced, and the subsequent homology search revealed that 201 corresponded to 180 known genes or known DNA sequences, which include not only housekeeping genes but also various tissue-specific genes. Interestingly, at least 24 of the 180 genes are development-related genes in mammals. Among these 24, those for midkine (growth and/or differentiation factor) and interferon-beta are reportedly up-regulated, and those for ECA39 (target for c-Myc regulation), REX-1 (zinc finger protein), and OCT-3 (POU-domain transcription factor) are down-regulated during the development of mouse embryonal carcinoma cells. Thirty-seven of the 582 clones matched the 36 previously reported unidentified ESTs (expressed sequence tags) and the remaining 344 corresponded to 329 novel ESTs. Therefore, partial sequencing of F9 cDNA clones corresponding to low-abundance mRNAs in F9 cells not only provides valuable information concerning development-related genes in mammals, but also many novel ESTs useful for studying mammalian genomes.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Nishiguchi S,Joh T,Horie K,Zou Z,Yasunaga T,Shimada Kdoi
10.1093/oxfordjournals.jbchem.a124485subject
Has Abstractpub_date
1994-07-01 00:00:00pages
128-39issue
1eissn
0021-924Xissn
1756-2651journal_volume
116pub_type
杂志文章abstract::Ubiquinone was biosynthesized when rat liver mitochondria were incubated with S-adenosyl-L-methionine, solanesyl diphosphate, and [U-14C]p-hydroxybenzoate. The intermediates of ubiquinone biosynthesis but not ubiquinone were accumulated in mitochondria incubated without S-adenosyl-L-methionine and the accumulated inte...
journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
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journal_title:Journal of biochemistry
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更新日期:1996-10-01 00:00:00
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更新日期:2005-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021757
更新日期:1997-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121919
更新日期:1987-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133807
更新日期:1982-04-01 00:00:00
abstract::Rat brain type II (beta) protein kinase C (PKC) was phosphorylated by rat lung casein kinase II (CK-II). Neither type I (gamma) nor type III (alpha) PKC was significantly phosphorylated by CK-II. CK-II incorporated 0.2-0.3 mol of phosphate into 1 mol of type II PKC. This phosphate was located at the single seryl resid...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123635
更新日期:1991-10-01 00:00:00
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更新日期:2018-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2020-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1986-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1982-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-03-01 00:00:00
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更新日期:1986-03-01 00:00:00
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更新日期:1985-01-01 00:00:00
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更新日期:1982-03-01 00:00:00
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更新日期:2021-01-21 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1989-03-01 00:00:00
abstract::Roxadustat (FG-4592, Rox) is a stabilizer for hypoxia-inducible transcription factors (HIFs), which induce production of the erythroid growth factor erythropoietin, and has been listed by the World Anti-Doping Agency as a prohibited substance for athletes since 2011. Although the detection technologies for Rox and its...
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更新日期:2018-02-09 00:00:00
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pub_type: 杂志文章
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更新日期:1976-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-07-01 00:00:00