Abstract:
:The aminopeptidase P gene in Escherichia coli HB101 was cloned into the plasmid pBR322. Introduction of the hybrid plasmid, pAPP01, into the E. coli DH1 resulted in an 8-fold increase of aminopeptidase P activity as compared with that of the host. The enzyme was purified by series of chromatographies on DEAE-Sephadex, QAE-Sephadex, and hydroxyapatite. The purified enzyme was homogeneous as judged by disc-gel and SDS-gel electrophoreses. the enzyme was inhibited strongly by EDTA and slightly by p-chloromercuribenzoate, but was not affected by diisopropyl phosphorofluoridate, E-64, or iodoacetic acid. The optimum pH of the enzyme was 8.5. The enzyme was stable at pH 8 to 9. After incubation for 30 min at pH 8.0, 50% remaining activity was observed at 50 degrees C. The enzyme was activated 3-fold by the addition of 5 microM Mn2+. The molecular weight of the enzyme was estimated to be 50,000 and 200,000 by SDS-PAGE and gel filtration, respectively. The amino terminal amino acid was identified to be serine by Edman degradation, indicating that the enzyme is composed of a homo-tetramer. The enzyme hydrolyzed X-Pro bonds (X = amino acid) of peptides. These characteristics suggest that cloned aminopeptidase P is identical to APP-II reported by Yoshimoto et al. (Agric. Biol. Chem. 52(8), in press (1988].
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Yoshimoto T,Murayama N,Honda T,Tone H,Tsuru Ddoi
10.1093/oxfordjournals.jbchem.a122430subject
Has Abstractpub_date
1988-07-01 00:00:00pages
93-7issue
1eissn
0021-924Xissn
1756-2651journal_volume
104pub_type
杂志文章abstract::The heparin-induced self-aggregation behaviours of four repeat peptides (R1-R4) in an acidic solution (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and compared with those in a neutral solution (pH = 7.5). In contrast with the self-aggregation-resistive behaviours of the R1 and ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvm099
更新日期:2007-07-01 00:00:00
abstract::The alpha-subunit of rod photoreceptor G protein transducin (G(t1)alpha) is heterogeneously modified at the N-terminus by a mixture of acyl groups, laurate (C12:0), myristate (C14:0), and two unsaturated fatty acids (C14:1 and C14:2). Although the N-fatty acylation of G(t1)alpha plays important roles in protein-protei...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvh039
更新日期:2004-03-01 00:00:00
abstract::The catalytic properties of a new type of dihydropteridine reductase, NADPH-specific dihydropteridine reductase [EC 1.6.99.10], from bovine liver, were studied and compared with those of the previously characterized enzyme, NADH-specific dihydropteridine reductase [EC 1.6.99.7]. With quinonoid-dihydro-6-methylpterin, ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135523
更新日期:1986-03-01 00:00:00
abstract::Partial amino acid sequences of two mitochondrial cytochrome P-450's, P-450 (SCC) and P-450 (11 beta), and two microsomal cytochrome P-450's, P-450 (C-21), and P-450 (17 alpha, Lyase), from adrenal cortex were analyzed and compared. Mitochondrial P-450's and microsomal P-450's were different in the amino acid sequence...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1983-11-01 00:00:00
abstract::Selected biochemical properties, including the charge heterodispersity profile and carbohydrate specificity, of bovine galectin-1 were determined in detail. The lectin was purified through an improved purification protocol that yielded 35-40 mg/kg of wet tissue with a specific activity of 1.7-2 x 10(4) mg-1.ml. The ga...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021493
更新日期:1996-11-01 00:00:00
abstract::The effect of crosslinking of F-actin with glutaraldehyde on the contractility of muscle ghost fiber containing reconstituted thin filament (i.e. F-actin-tropomyosin-troponin complex) and irrigated with myosin was investigated. The results show that: (i) crosslinking inhibited development of isometric tension and shor...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134045
更新日期:1982-10-01 00:00:00
abstract::The amino acid compositions, constituent polypeptides and bacteriochlorophyll (Bchl) contents of two kinds of Bchl-protein complexes isolated from Rhodopseudomonas palustris were examined. Spectrally dissimilar intracytoplasmic membranes obtained from cells cultured under different conditions were used as starting mat...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134110
更新日期:1982-12-01 00:00:00
abstract::We studied the mechanism of formation of oxygen radicals during ferrous ion-induced decomposition of linoleic acid hydroperoxide using the spin trapping and chemiluminescence methods. The formation of the superoxide anion (O2*-) was verified in the present study. The hydroxyl radical is also generated through Fenton t...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvg217
更新日期:2003-12-01 00:00:00
abstract::Cell metabolism can be considered as a process of serial construction and destruction of cellular components, both of which must be regulated accurately. In eukaryotic cells, a variety of cellular components are actively delivered into lysosomes/vacuoles, specialized compartments for hydrolysis of macromolecules. Such...
journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
doi:10.1093/jb/mvw085
更新日期:2017-02-01 00:00:00
abstract::Vasoactive intestinal peptide (VIP), a neuropeptide coupled with adenylate cyclase, was found to induce neurite extension of PC12h cells. Neurites appeared within 1 h after addition of VIP and extended for at least 24 h. The half-maximal concentration for the effect of VIP was 50 nM. In addition to the morphological c...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124333
更新日期:1994-02-01 00:00:00
abstract::An esterase that specifically hydrolyzes medium-chain (C(3)-C(5)) monoalkyl phthalates was purified from phthalate-grown Micrococcus sp. YGJ1. The enzyme activity was split into two fractions by hydrophobic chromatography on Phenyl Sepharose, and the enzymes were purified to homogeneity from each fraction. The purifie...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvi004
更新日期:2005-01-01 00:00:00
abstract::Three forms of cytochrome P-450 of liver microsomes of 3-methylcholanthrene-treated Golden hamsters were purified and characterized as regards their catalytic activity toward aflatoxin B1-related hepatocarcinogenic mycotoxins. These include two major forms, designated as cytochrome P-450-AFB (P-450-I) and P-450-II, an...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122840
更新日期:1989-08-01 00:00:00
abstract::The lanthanide ions Lu3+ (diamagnetic) and Gd3+ (paramagnetic broadening probe) were used to displace Ca2+ from the high-affinity cation binding site on G-actin. The effects of these higher-affinity ions on the proton nuclear magnetic resonance spectrum of actin were recorded. The aliphatic proton envelope in the Gd-a...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134910
更新日期:1984-09-01 00:00:00
abstract::The plasma membrane, the outermost surface of eukaryotic cells, contains various substructures, such as protrusions or invaginations, which are associated with diverse functions, including endocytosis and cell migration. These structures of the plasma membrane can be considered as tubules or inverted tubules (protrusi...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvq049
更新日期:2010-07-01 00:00:00
abstract::Under conditions where nM level of calmodulin was able to show full activation of myosin light chain kinase and cyclic-nucleotide phosphodiesterase, the fragments of calmodulin at concentrations as high as 20 microM failed to activate these enzymes in the presence of Ca2+. The fragments tested were Ala1-Lys75 (F12), A...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122303
更新日期:1988-03-01 00:00:00
abstract::In order to establish cell lines which complement the growth of temperature-sensitive (ts) mutants of influenza virus, three RNA polymerase and nucleoprotein (NP) genes each linked to the mouse mammary tumor virus LTR were cloned into the bovine papillomavirus vector DNA. After co-transfection of mouse C127 cells with...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123592
更新日期:1991-09-01 00:00:00
abstract::Plant cell-wall arabinoxylans have a complex structure that requires the action of a pool of debranching (arabinofuranosidases) and depolymerizing enzymes (endo-xylanase). Two Aspergillus nidulans strains over-secreting endo-xylanase and arabinofuranosidase were inoculated in defined 2% maltose-minimum medium resultin...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvt053
更新日期:2013-09-01 00:00:00
abstract::Incubation of Sarcophaga fat bodies in buffered insect saline resulted in simultaneous activation of multiple defense protein genes [Sugiyama, H. and Natori, S. (1991) Eur. J. Biochem. 200, 495-500]. In this study, we demonstrated that the addition of both adenosine and homocysteine thiolactone to this medium abolishe...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021247
更新日期:1996-02-01 00:00:00
abstract::X-ray diffraction photographs of a chicken gizzard G-actin.DNase I complex crystal have been recorded using the synchrotron radiation beam emitted by the Synchrotron Radiation Source at Daresbury and the Photon Factory at Tsukuba. The resolution limit was extended to 2.4 A and the exposure time was reduced approximate...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134682
更新日期:1984-03-01 00:00:00
abstract::Rabbit skeletal muscle alpha-tropomyosin (Tm) and the deletion mutant (D234Tm) in which internal actin-binding pseudo-repeats 2, 3, and 4 are missing [Landis et al. (1997) J. Biol. Chem. 272, 14051-14056] were used to investigate the interaction between actin and tropomyosin or actin and troponin (Tn) by means of fluo...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003116
更新日期:2002-03-01 00:00:00
abstract::We have examined critically whether or not new and old histones mix in the octameric units of nucleosomes during chromatin replication. MH-134SC cells were density-labeled by culturing with amino acid mixtures enriched with dense isotopes 2H, 13C, and 15N. Mononucleosomes obtained from labeled cells were fractionated ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121946
更新日期:1987-04-01 00:00:00
abstract::Lambda dv plasmids having an amber mutation in an initiator gene, O or P, were constructed from mutant lambda phages by recombinant DNA techniques and several properties of such derivatives were investigated. These plasmids are perpetuated in suppressor-plus (amber-permissive) cells, but not in non-suppressor cells. T...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134360
更新日期:1983-08-01 00:00:00
abstract::Diacylglycerol lipase (DGL) was solubilized from human platelet microsomes with heptyl-beta-D-thioglucoside, and purified to homogeneity on SDS-PAGE using a combination of chromatographic and electrophoretic methods. The molecular mass of the purified DGL was estimated to be 33 kDa. Its apparent pI was pH 6.0, as dete...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022389
更新日期:1999-06-01 00:00:00
abstract::The complement system is a classic central player in innate immunity. Most pathogens activate both complement and the toll-like receptor (TLR) pathway. Therefore, to provide a more comprehensive understanding of innate immunity, it is important to understand the crosstalk between these two systems. Mouse macrophages p...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvq136
更新日期:2011-03-01 00:00:00
abstract::Bdellin-KL is a trypsin-plasmin inhibitor from Hirudo nipponia, whose N-terminal sequence was identified as a non-classical Kazal-type. A cDNA clone encoding the inhibitor was isolated by reverse transcription-PCR and 5' rapid amplification of cDNA ends. The cDNA showed an open reading frame of 155 amino acids compris...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003003
更新日期:2001-09-01 00:00:00
abstract::Poly(8-oxyinosinic acid) (poly O8I), was synthesized by polymerizing 8-oxyinosine diphosphate using the enzyme polynucleotide phosphorylase (PNPase). The polymer formed a 1 : 1 hybrid with polycytidylic acid (poly C). The hybrid was found to induce the production of interferon in the brain of white albino mice and pro...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134012
更新日期:1982-09-01 00:00:00
abstract::Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organell...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124320
更新日期:1994-02-01 00:00:00
abstract::The genes coding for the phospholipid degradation enzymes in E. coli, detergent-resistant (DR-) phospholipase A (pldA) and lysophospholipase L2 (pldB), were cloned together on the plasmid pKO1 (Homma, H., Kobayashi, T., Ito, Y., Kudo, I., Inoue, K., Ikeda, H., Sekiguchi, M., & Nojima, S. (1983) J. Biochem. 94, 2079-20...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135346
更新日期:1985-10-01 00:00:00
abstract::Mitochondrial porin is a major integral membrane protein of the outer membrane. To assess the stop-transfer sequence in the yeast porin molecule (P), we constructed the following chimeric proteins. (i) The signal sequence of interleukin 2, a secretory protein, was fused to the amino-terminus of porin (SP). (ii) The ma...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123884
更新日期:1992-08-01 00:00:00
abstract::FOXP2 is a member of the P subfamily of FOX transcription factors, the DNA-binding domain of which is the winged helix forkhead domain (FHD). In this work we show that the FOXP2 FHD is able to bind to various DNA sequences, including a novel sequence identified in this work, with different affinities and rates as dete...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvx003
更新日期:2017-07-01 00:00:00