The proposed functions of membrane curvatures mediated by the BAR domain superfamily proteins.

abstract：:Alkaline phosphatase was entrapped in reverse micelles formed in n-heptane with surfactants (bis(2-ethylhexyl)sodium sulfosuccinate, phosphatidylcholine, phosphatidylethanolamine, or phosphatidic acid) and water. The entrapped enzyme could express its activity in the reverse micelles of the above surfactants only when...

journal_title：Journal of biochemistry

authors： Ohshima A,Narita H,Kito M

更新日期：1983-05-01 00:00:00

abstract：:Two G-protein-coupled receptors (GPCRs) that couple with Gαq/11, B2 bradykinin (BK) receptor (B2R) and ATP/UTP receptor P2Y2 (P2Y2R), are ubiquitously expressed and responsible for vascular tone, inflammation, and pain. We analysed the cellular signalling of P2Y2Rs in cells that express B2Rs. B2R desensitization induc...

journal_title：Journal of biochemistry

authors： Yashima S,Shimazaki A,Mitoma J,Nakagawa T,Abe M,Yamada H,Higashi H

更新日期：2015-08-01 00:00:00

abstract：:Calmodulin (CaM), a physiologically important Ca(2+)-binding protein, participates in numerous cellular regulatory processes. It is dumbbell shaped and contains two globular domains connected by a short alpha-helix. Each of the globular domains has two Ca(2+)-binding sites, the EF hands. CaM undergoes a conformational...

journal_title：Journal of biochemistry

authors： Shishido H,Yamada MD,Kondo K,Maruta S

更新日期：2009-10-01 00:00:00

abstract：:Methyl-coenzyme M reductase isoenzyme I from the methanogenic Archaeon, Methanobacterium thermoautotrophicum (strain Marburg), was crystallized by vapor diffusion methods. Crystal form M obtained with 2-methyl-2,4-pentanediol as the precipitant displayed space group P2(1), with unit cell parameters of a=83.2 A, b=117....

journal_title：Journal of biochemistry

authors： Shima S,Goubeaud M,Vinzenz D,Thauer RK,Ermler U

更新日期：1997-05-01 00:00:00

abstract：:The minimal structural unit of cytochrome c oxidase purified from Thiobacillus novellus was composed of one molecule each of two subunits with molecular masses of 32 and 23 kDa, respectively, and the unit had one molecule of heme a and one atom of copper. In the presence of n-octyl-beta-D-thioglucoside, the oxidase ex...

journal_title：Journal of biochemistry

authors： Shoji K,Yamazaki T,Nagano T,Fukumori Y,Yamanaka T

更新日期：1992-01-01 00:00:00

abstract：:Pseudomonas putida PpGl, which carries the CAM plasmid encoding enzymes involved in the degradation pathway of D-camphor, can utilize D-camphor as a sole carbon source. Cytochrome P-450cam and related enzymes participate in the early oxidation steps of D-camphor degradation metabolism. We cloned from a HindIII DNA lib...

journal_title：Journal of biochemistry

authors： Koga H,Yamaguchi E,Matsunaga K,Aramaki H,Horiuchi T

更新日期：1989-11-01 00:00:00

abstract：:Bovine dopamine beta-monooxygenase is a tetramer consisting of two disulfide-linked dimers. To examine the role(s) of the intersubunit disulfide bonds in the protein structure and activity, the enzyme was treated with DTT at pH 7.5 and 25 degrees C under nondenaturing conditions. A 15-min incubation with 0.5 mM DTT se...

journal_title：Journal of biochemistry

authors： Ishida T,Narita M,Nozaki M,Horiike K

更新日期：1996-08-01 00:00:00

abstract：:Picolinate binds to a reduced form of D-amino acid oxidase, and the complex formed has a broad absorption band around 600 nm as in the case of the purple intermediate of the enzyme with a substrate. The dissociation constant at 25 degrees C was 35 microns at pH 7.0. The pH dependence (pH 8.3-pH 6.4) of the dissociatio...

journal_title：Journal of biochemistry

authors： Nishina Y,Tojo H,Shiga K

更新日期：1986-03-01 00:00:00

abstract：:The melanocortin receptor type 4 (MC4-R) is involved in food intake and represents a potential target for the treatment of some forms of obesity. The fluorescent protein EGFP was fused to the wild-type or mutated coding sequence of the human MC4-R. After transfection in HEK 293, clones stably expressing hMC4-R-EGFP we...

journal_title：Journal of biochemistry

authors： Blondet A,Doghman M,Rached M,Durand P,Bégeot M,Naville D

更新日期：2004-04-01 00:00:00

abstract：:The DegS-DegU two-component regulatory system regulates many cellular events in Bacillus subtilis. Genes for DegSU constitutes an operon directed by the P1 promoter and downstream degU is autoregulated via the P3 promoter activated by phosphorylated DegU. In the Gram-positive bacteria, Spx plays a major role in the pr...

journal_title：Journal of biochemistry

authors： Shiwa Y,Yoshikawa H,Tanaka T,Ogura M

更新日期：2015-05-01 00:00:00

abstract：:A protein, which was immunoreactive to antibody against cytochrome c oxidase, was found in the mitochondrial membrane fraction of sweet potato root tissue. The protein was associated relatively weakly with the mitochondrial inner membrane as compared with cytochrome c oxidase. It exerted no cytochrome c oxidase activi...

journal_title：Journal of biochemistry

authors： Maeshima M,Asahi T

更新日期：1981-08-01 00:00:00

abstract：:Bone marrow lineage-negative (Lin(-)) c-Kit(+) Sca-1(+) hematopoietic cells from human GM-CSF receptor gene transgenic mice were cultured on established bone marrow stromal cell (TBR59) layers and on semisolid medium. In the semisolid assay, an increasing number of larger colonies were observed in the presence of hGM-...

journal_title：Journal of biochemistry

authors： Okubo T,Yanai N,Watanabe S,Arai Ki,Obinata M

更新日期：2000-04-01 00:00:00

abstract：:Raman spectroscopy was used to investigate the hydrogen bonding at the C(4)=O moiety of the isoalloxazine nucleus in a series of flavins and flavoproteins. Isotope effects of Raman bands confirmed that the band observed around 1,710 cm(-1) is mainly derived from C(4)=O stretching vibrational mode. A linear correlation...

journal_title：Journal of biochemistry

authors： Hazekawa I,Nishina Y,Sato K,Shichiri M,Miura R,Shiga K

更新日期：1997-06-01 00:00:00

abstract：:MscL is a mechanosensitive channel that undergoes a global conformational change upon application of membrane stretching. To elucidate how the structural stability and flexibility occur, we isolated temperature-sensitive (Ts) mutants of Escherichia coli MscL that allowed cell growth at 32°C but not at 42°C. Two Ts mut...

journal_title：Journal of biochemistry

authors： Owada N,Yoshida M,Morita K,Yoshimura K

更新日期：2019-09-01 00:00:00

abstract：:Carboxypeptidase Y (CPY) inhibitor, I(C), a cytoplasmic inhibitor of vacuolar proteinases in yeast, Saccharomyces cerevisiae, was purified by means of a high-level expression system using a proteinase-deficient strain, BJ2168, and an expression vector with the promoter GAL1. The purified I(C) exists as a monomeric bet...

journal_title：Journal of biochemistry

authors： Mima J,Suzuki H,Takahashi M,Hayashi R

更新日期：2002-12-01 00:00:00

abstract：:An escape mutant of human parainfluenza virus type 1 (hPIV1), which was selected by serial passage in the presence of a sialidase inhibitor, 4-O-thiocarbamoylmethyl-2-deoxy-2,3-didehydro-N-acetylneur-aminic acid (TCM-Neu5Ac2en), exhibited remarkable syncytium formation and virus-induced cell death in LLC-MK2 cells but...

journal_title：Journal of biochemistry

authors： Takaguchi M,Takahashi T,Hosokawa C,Ueyama H,Fukushima K,Hayakawa T,Itoh K,Ikeda K,Suzuki T

更新日期：2011-02-01 00:00:00

abstract：:Spontaneous insertion of membrane proteins into liposomes formed from Escherichia coli polar phospholipids is blocked by diacylglycerol (DAG) at a physiological level. We found that cholesterol also blocks this spontaneous insertion, although a much larger amount is necessary for sufficient blockage. Reversely, sphing...

journal_title：Journal of biochemistry

authors： Nakamura S,Suzuki S,Saito H,Nishiyama KI

更新日期：2018-04-01 00:00:00

abstract：:Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry 20, 2945-2950) that these fragments can be separated by gel filtration i...

journal_title：Journal of biochemistry

authors： Hozumi T

更新日期：1981-09-01 00:00:00

abstract：:Arginine methylation in RNA-binding proteins containing arginine- and glycine-rich RGG motifs is catalyzed by specific protein arginine N-methyltransferase in cells. We previously showed that lymphoblastoid cells grown in the presence of an indirect methyltransferase inhibitor, adenosine dialdehyde (AdOx), accumulated...

journal_title：Journal of biochemistry

authors： Lin CH,Hsieh M,Li YC,Li SY,Pearson DL,Pollard KM,Li C

更新日期：2000-09-01 00:00:00

abstract：:LukF and Hlg2 of staphylococcal gamma-hemolysin assemble into hetero-oligomeric pores on human red blood cells (HRBC). Here, we demonstrate, using a single-molecule imaging technique, that a W177T/R198T mutant of LukF, which exhibits no binding activity toward phosphatidylcholine, could form intermediate oligomers wit...

journal_title：Journal of biochemistry

authors： Monma N,Nguyen VT,Kaneko J,Higuchi H,Kamio Y

更新日期：2004-10-01 00:00:00

abstract：:S-RNase is associated with the gametophytic self-incompatibility of flowering plants in Solanaceae and, on the basis of sequence homology, belongs to the RNase T2 family. To identify the active site residues in S-RNase, Nicotiana alata S6-RNase was studied by chemical modification. S6-RNase was inactivated with iodoac...

journal_title：Journal of biochemistry

authors： Ishimizu T,Miyagi M,Norioka S,Liu YH,Clarke AE,Sakiyama F

更新日期：1995-11-01 00:00:00

abstract：:Kinetic parameters, Km and Vmax for the transesterification of oligouridylic acid, (Up)nU greater than p (n=0-4), by RNase A were measured spectrophotometrically at pH 7.0 and 25 degrees C. The kinetic parameters, pKm and log Vmax increased with increase in the chain length (n), and seemed to be almost constant with s...

journal_title：Journal of biochemistry

authors： Irie M,Mikami F,Monma K,Ohgi K,Watanabe H,Yamaguchi R,Nagase H

更新日期：1984-07-01 00:00:00

abstract：:Substrate specificities of phospholipases C[EC 3.1.4.3] from Clostridium novyi, Clostridium perfringens, Bacillus cereus, and Pseudomonas aureofaciens were studied under the same conditions. Phospholipases C from Clostridium novyi and Bacillus cereus show wide substrate specificities while those of Clostridium perfrin...

journal_title：Journal of biochemistry

authors： Taguchi R,Ikezawa H

更新日期：1977-11-01 00:00:00

abstract：:Tardigrades, a phylum of meiofaunal organisms, exhibit extraordinary tolerance to various environmental conditions, including extreme temperatures (-272 to 151 °C) and exposure to ionizing radiation. Proteins from anhydrobiotic tardigrades with homology to known proteins from other organisms are new potential targets ...

journal_title：Journal of biochemistry

authors： Kobayashi K,Kim J,Fukuda Y,Kozawa T,Inoue T

更新日期：2021-01-21 00:00:00

abstract：:A cDNA for mouse prolyl endopeptidase (PEP) was cloned and its nucleotide sequence determined. The overall amino acid sequence identity between mouse and other mammalian PEPs was about 96%. A specific inhibitor of PEP, N-benzyloxycarbonyl-thioprolyl-thioprolinal- dimethylacetal (ZTTA), inhibited DNA synthesis by Swiss...

journal_title：Journal of biochemistry

authors： Ishino T,Ohtsuki S,Homma Ki,Natori S

更新日期：1998-03-01 00:00:00

abstract：:RPE65 is a membrane-associated retinoid isomerase involved in the visual cycle responsible for sustaining vision. Many mutations in the human RPE65 gene are associated with distinct forms of retinal degenerative diseases. The pathogenic mechanisms for most of these mutations remain poorly understood. Here, we show tha...

journal_title：Journal of biochemistry

authors： Li S,Hu J,Jin RJ,Aiyar A,Jacobson SG,Bok D,Jin M

更新日期：2015-08-01 00:00:00

abstract：:The phospholipid mediator, platelet activating factor (PAF: 1-O-alkyl-2-O-acetyl-sn-glycero-3-phosphocholine), was recently reported to activate macrophage-monocyte cells as well as neutrophils or platelets. PAF was incorporated into dipalmitoylphosphatidylcholine-cholesterol liposomes, and their effect on guinea pig ...

journal_title：Journal of biochemistry

authors： Hayashi H,Kudo I,Inoue K,Nomura H,Nojima S

更新日期：1985-04-01 00:00:00

abstract：:Neutral ceramidase (NCDase) is considered to be a critical enzyme for controlling the turnover of ceramide, an important bioactive lipid, which determines cell's fate. All-trans retinoic acid (ATRA) has been reported to induce neuronal differentiation and cell-cycle arrest [Lopez-Carballo, Moreno, Masia, Perez, and Ba...

journal_title：Journal of biochemistry

authors： Tanaka K,Tamiya-Koizumi K,Hagiwara K,Ito H,Takagi A,Kojima T,Suzuki M,Iwaki S,Fujii S,Nakamura M,Banno Y,Kannagi R,Tsurumi T,Kyogashima M,Murate T

更新日期：2012-06-01 00:00:00

abstract：:It was shown that a dipeptide, alanylhistidine, can act as a catalyst for the peptidyl transfer reaction in the absence of ribosomes between the amino acid moieties of phenylalanyl, lysyl, prolyl, and glycyl tRNAs depending on their model templates, poly U, poly A, poly C, and poly G, respectively. A template effect w...

journal_title：Journal of biochemistry

authors： Shimizu M

更新日期：1996-05-01 00:00:00

abstract：:Serum mannan binding protein (MBP), a mannose/N-acetylglusosamine-specific lectin, is important in innate immunity. In order to elucidate the mechanism underlying the wide intra- and interracial variety in the MBP serum level, we have studied the transcriptional regulation of human MBP. Rapid amplification of cDNA end...

journal_title：Journal of biochemistry

authors： Naito H,Ikeda A,Hasegawa K,Oka S,Uemura K,Kawasaki N,Kawasaki T

更新日期：1999-12-01 00:00:00