The environment of the high-affinity cation binding site on actin and the separation between cation and ATP sites as revealed by proton NMR and fluorescence spectroscopy.

abstract：:To identify the residues modified by radioactive N6-p-bromoacetaminobenzyladenosine-5'-phosphate in phosphorylase b, the peptic peptides of the modified enzyme were purified. Two radioactive peptides were isolated, each of which had a cysteine residue modified. The cysteine residue involved in the AMP site was determi...

journal_title：Journal of biochemistry

authors： Kawashima S,Eguchi C,Imahori K

更新日期：1978-12-01 00:00:00

abstract：:The 5'-AMP-activated protein kinase (AMPK) functions as a cellular energy sensor. 5-Aminoimidazole-4-carboxyamide-1-β-D-ribofranoside (AICAR) is a chemical activator of AMPK. In the liver, AICAR suppresses expression of thephosphoenolpyruvate carboxykinase(PEPCK) gene. The rat enhancer of split- and hairy-related prot...

journal_title：Journal of biochemistry

authors： Komatsu Y,Yanagisawa Y,Moriizumi M,Tsuchiya Y,Yokouchi H,Otsuka H,Aoyagi M,Tsukada A,Kanai Y,Haneishi A,Takagi K,Asano K,Ono M,Tanaka T,Tomita K,Yamada K

更新日期：2016-04-01 00:00:00

abstract：:A small nuclear protein, C1D, has roles in various cellular processes, transcription regulation, genome stability surveillance, DNA repair and RNA processing, all of which are required to maintain the host life cycles. In the previous report, C1D directly interacts with XPB, a component of the nucleotide excision repa...

journal_title：Journal of biochemistry

authors： Tomita T,Ieguchi K,Takita M,Tsukahara F,Yamada M,Egly JM,Maru Y

更新日期：2018-12-01 00:00:00

abstract：:The substrate specificity of microsomal nonspecific carboxyesterase [EC 3.1.1.1] from rabbit and rat livers was studied in vitro by using seven (A)-type and six (B)-type 12,13-epoxytrichothecene mycotoxins. The C-4 acetyl residues of diacetoxyscirpenol, T-2 toxin, monoacetylnivalenol (fusarenon-X), and diacetylnivalen...

journal_title：Journal of biochemistry

authors： Ohta M,Matsumoto H,Ishii K,Ueno Y

更新日期：1978-09-01 00:00:00

abstract：:Interferon stimulated gene factor 3 (ISGF3) is a trimeric transcription factor activated on treatment of cells with interferon-alpha and beta (type I IFNs). Upon stimulation, the regulatory subunits, p84/91 and p113, present in the cytoplasm are phosphorylated at specific tyrosine residues and assemble with the DNA bi...

journal_title：Journal of biochemistry

authors： Suhara W,Yoneyama M,Yonekawa H,Fujita T

更新日期：1996-02-01 00:00:00

abstract：:The complete amino acid sequence of Fragment B obtained by the limited tryptic digestion of E. coli polypeptide chain elongation factor Tu (EF-Tu) was determined. Seven peptides formed from Fragment B by cleavage with cyanogen bromide (designated as CB1 to CB7 according to their order of alignment from N- to C-termini...

journal_title：Journal of biochemistry

authors： Nakamura S,Nakayama N,Takahashi K,Kaziro Y

更新日期：1982-03-01 00:00:00

abstract：:A protease activity specific to spermatogenous histones was found in the egg extract of sea urchin. The enzyme responsible for this activity, named SPKK protease because of its substrate specificity, was purified as a monomeric 28 kDa protein. SPKK protease activity is inhibited by leupeptin and is specific to the rep...

journal_title：Journal of biochemistry

authors： Suzuki M,Sugiura M,Ebashi S

更新日期：1990-09-01 00:00:00

abstract：:Glycoengineering of therapeutic proteins has been applied to improve the clinical efficacy of several therapeutics. Here, we examined the effect of glycosylation on the properties of the Fab of the therapeutic antibody, adalimumab. An N-glycosylation site was introduced at position 178 of the H-chain constant region o...

journal_title：Journal of biochemistry

authors： Nakamura H,Kiyoshi M,Anraku M,Hashii N,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2020-10-27 00:00:00

abstract：:Serum at 5 to 10% is required for maintenance of functional adult rat hepatocytes in primary culture. One effect of the serum is to induce attachment and spreading of hepatocytes on plates as monolayers. Another role is to maintain cell viability for over 2 days. For the first effect, serum could be replaced completel...

journal_title：Journal of biochemistry

authors： Horiuti Y,Nakamura T,Ichihara A

更新日期：1982-12-01 00:00:00

abstract：:Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly req...

journal_title：Journal of biochemistry

authors： Endo Y,Nagai H,Watanabe Y,Ochi K,Takagi T

更新日期：1992-11-01 00:00:00

abstract：:E. coli bearing hybrid plasmid pKOl (Oeda et al. (1981) Mol. Gen. Genet. 184, 191-199) expressed a large amount of lysophospholipase L2 activity. When a mutant which was defective in lysophospholipase L2 activity was transformed with plasmid pKOl, it overproduced lysophospholipase L2 activity. The gene responsible for...

journal_title：Journal of biochemistry

authors： Homma H,Kobayashi T,Ito Y,Kudo I,Inoue K,Ikeda H,Sekiguchi M,Nojima S

更新日期：1983-12-01 00:00:00

abstract：:The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rate...

journal_title：Journal of biochemistry

authors： Shiota M,Chinzei F,Moriyama M,Kimura K,Sugano T

更新日期：1995-05-01 00:00:00

abstract：:The heparin-induced self-aggregation behaviours of four repeat peptides (R1-R4) in an acidic solution (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and compared with those in a neutral solution (pH = 7.5). In contrast with the self-aggregation-resistive behaviours of the R1 and ...

journal_title：Journal of biochemistry

authors： Mizushima F,Minoura K,Tomoo K,Sumida M,Taniguchi T,Ishida T

更新日期：2007-07-01 00:00:00

abstract：:A chromosomal gene, mlr6793, in Mesorhizobium loti was identified as the gene encoding 5-formyl-3-hydroxy-2-methylpyridine 4-carboxylic acid (FHMPC) dehydrogenase (dismutase) involved in the degradation pathway for pyridoxine (vitamin B(6)). The homogenously purified recombinant enzyme has a molecular mass of 59.1 kDa...

journal_title：Journal of biochemistry

authors： Yokochi N,Yoshikane Y,Matsumoto S,Fujisawa M,Ohnishi K,Yagi T

更新日期：2009-04-01 00:00:00

abstract：:The DNA-dependent protein kinase (DNA-PK) holoenzyme consists of a 470-kDa catalytic subunit (DNA-PKcs), a DNA-binding regulatory component known as Ku protein, and double-stranded DNA (dsDNA) with ends. We previously reported that the activity of DNA-PK in vitro is stimulated by non-histone chromosomal high mobility ...

journal_title：Journal of biochemistry

authors： Yumoto Y,Shirakawa H,Yoshida M,Suwa A,Watanabe F,Teraoka H

更新日期：1998-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of ∼22 nt non-coding RNAs that control diverse biological functions in animals, plants and unicellular eukaryotes by promoting degradation or inhibition of translation of target mRNAs. miRNA expression is often tissue specific and developmentally regulated. Aberrant expression of miRNAs ...

journal_title：Journal of biochemistry

authors： Davis-Dusenbery BN,Hata A

更新日期：2010-10-01 00:00:00

abstract：:The internal structure of the recombinant yeast-derived human hepatitis B virus surface antigen vaccine particle was investigated by small-angle neutron scattering using the contrast variation method. Data were collected in aqueous buffer solutions containing 0, 40, 60, and 100% D2O in the q range of 0.005 to 0.2 A-1 ...

journal_title：Journal of biochemistry

authors： Sato M,Sato-Miyamoto Y,Kameyama K,Ishikawa N,Imai M,Ito Y,Takagi T

更新日期：1995-12-01 00:00:00

abstract：:Anti-angiotensin I IgG in serum was measured by immune complex transfer enzyme immunoassays using angiotensin I conjugates prepared by two different methods. In the first method, angiotensin I was conjugated to dinitrophenyl bovine serum albumin and beta-D-galactosidase through covalent links. Anti-angiotensin I IgG i...

journal_title：Journal of biochemistry

authors： Kohno T,Yamaguchi K,Ishikawa E

更新日期：1990-11-01 00:00:00

abstract：:Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ de...

journal_title：Journal of biochemistry

authors： Tanaka Y,Maruyama K,Mikawa T,Hotta Y

更新日期：1988-10-01 00:00:00

abstract：:The E2F transcription factors (TFs), which control the progression of the cell cycle in response to DNA-damage and various stresses, are known to interact with a tumor suppressor, Retinoblastoma 1 (RB1). We previously showed that the response of the human RB1 promoter to a 12-O-tetradecanoylphorbol-13-acetate (TPA) in...

journal_title：Journal of biochemistry

authors： Hamada H,Goto Y,Arakawa J,Murayama E,Ogawa Y,Konno M,Oyama T,Asai M,Sato A,Tanuma SI,Uchiumi F

更新日期：2019-06-14 00:00:00

abstract：:Palmitoyl CoA inhibited EDTA-ATPase of heavy meromyosin (HMM) prepared from rabbit skeletal muscle. The concentration for half maximum inhibition of EDTA-ATPase was about 18 microM. Myristoyl CoA, the other long chain fatty acyl CoA, also inhibited EDTA-HMM ATPase, but CoA and short chain CoA thioesters, such as butyr...

journal_title：Journal of biochemistry

authors： Fujiwara A,Fujisaki H,Asai H,Yasumasu I

更新日期：1981-09-01 00:00:00

abstract：:Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC 3.1.4.34] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

journal_title：Journal of biochemistry

authors： Sawai Y,Yanokura M,Tsukada K

更新日期：1979-09-01 00:00:00

abstract：:The ATPase inhibitor from yeast, Saccharomyces cerevisiae, was found to strongly inhibit the ATPase activity when it was preincubated in an acidic pH region. This paper describes the conformational changes of the inhibitor in the acidic pH region, as studied by 1H NMR spectroscopy. In the pH range from 7.43 to 4.48, t...

journal_title：Journal of biochemistry

authors： Fujii S,Hashimoto T,Yoshida Y,Miura R,Yamano T,Tagawa K

更新日期：1983-01-01 00:00:00

abstract：:Human serum was shown to contain N-acetyllactosamine: N-acetylglucosaminyltransferase activity. The reaction product was hydrolyzed by beta-N-acetylglucosaminidase and released [14C]N-acetylglucosamine, indicating that the N-acetylglucosaminyl residue was beta-linked to N-acetyllactosamine. Methylation and hydrolysis ...

journal_title：Journal of biochemistry

authors： Hosomi O,Takeya A,Kogure T

更新日期：1984-06-01 00:00:00

abstract：:Bacillus licheniformis alpha-amylase (BLA) is widely used in various procedures of starch degradation in the food industry, and a BLA species with improved activity at higher temperature and under acidic conditions is desirable. Two BLA species, designated as PA and MA, have been isolated from the wild-type B. licheni...

journal_title：Journal of biochemistry

authors： Lee S,Oneda H,Minoda M,Tanaka A,Inouye K

更新日期：2006-06-01 00:00:00

abstract：:A rapid and simple method was developed for the purification of serine hydroxymethyltransferases [EC 2.1.2.1]. The procedure involved ammonium sulfate precipitation, DEAE-cellulose column chromatography and affinity chromatography on an L-adsorbent. Through this procedure the cytosolic enzyme (s-SHMT) was purified 1,6...

journal_title：Journal of biochemistry

authors： Masuda T,Sakamoto M,Nishizaki I,Hayashi H,Yamamoto M,Wada H

更新日期：1987-03-01 00:00:00

abstract：:Sodium- and potassium-activated adenosinetriphosphatase (Na+, K+-ATPase) purified from dog kidney outer medulla was examined by polyacrylamide gel electrophoresis and by photoaffinity labeling with N-(ouabain)-N'-(2-nitro-4-azidophenyl)-ethylenediamine (NAP-ouabain). The large subunit band (alpha-band) split into two ...

journal_title：Journal of biochemistry

authors： Ohta T,Kawamura M,Hasegawa T,Ishikura H,Nagano K

更新日期：1982-01-01 00:00:00

abstract：:MF-18, one of the monoclonal antibodies generated to chicken myosin, cross-reacted with rabbit skeletal myosin subfragment-1 (S1). Utilizing an improved procedure of immuno-blotting, a decrease in reactivity of MF-18 to S1 by trinitrophenylation was observed. This indicates that the reactive lysyl residue is very clos...

journal_title：Journal of biochemistry

authors： Katayama E,Wakabayashi T,Reinach F,Masaki T,Fischman DA

更新日期：1984-03-01 00:00:00

abstract：:A large imprinted gene cluster in human chromosome 11p15.5 has been implicated in Beckwith-Wiedemann syndrome and Wilms' tumor. We have identified a paternally expressed imprinted gene, PEG8/IGF2AS, in this locus. It is transcribed in the opposite direction to the IGF2 transcripts and some genomic regions are shared w...

journal_title：Journal of biochemistry

authors： Okutsu T,Kuroiwa Y,Kagitani F,Kai M,Aisaka K,Tsutsumi O,Kaneko Y,Yokomori K,Surani MA,Kohda T,Kaneko-Ishino T,Ishino F

更新日期：2000-03-01 00:00:00

abstract：:Sliding machines composed of F-actin and myosin or microtubules and kinesin or dynein convert the free energy of ATP hydrolysis into sliding movements and mechanical work. Development of optical microscopy with micromanipulation techniques has made possible direct observation of single events of sliding exhibited by s...

journal_title：Journal of biochemistry

authors： Oosawa F

更新日期：1995-11-01 00:00:00