Multiple forms of ribonuclease H from rat liver cytosol.

abstract：:A spectrofluorometric method for sensitive determination of metalloendopeptidase activity has been developed by using a bimane-peptide containing a tryptophan residue, i.e. 1,7-dioxo-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-alpha]pyrazol-3-yl-methyl- thiomethylcarbonyl-phenylalanyl-tryptophanyl-leucine (Bim-SCH2CO-Phe-Trp-L...

journal_title：Journal of biochemistry

authors： Kajiwara K,Kumazaki T,Sato E,Kanaoka Y,Ishii S

更新日期：1991-09-01 00:00:00

abstract：:MicroRNAs (miRNAs) are a class of ∼22 nt non-coding RNAs that control diverse biological functions in animals, plants and unicellular eukaryotes by promoting degradation or inhibition of translation of target mRNAs. miRNA expression is often tissue specific and developmentally regulated. Aberrant expression of miRNAs ...

journal_title：Journal of biochemistry

authors： Davis-Dusenbery BN,Hata A

更新日期：2010-10-01 00:00:00

abstract：:The Tetrahymena group I intron ribozyme folds into a complex three dimensional structure for performing the self-splicing reaction. Catalysis depends on its core structure comprising two helical domains, P4-P6 and P3-P7. The two domains are joined by three sets of conserved base-triple(s) and other tertiary interactio...

journal_title：Journal of biochemistry

authors： Ohki Y,Ikawa Y,Shiraishi H,Inoue T

更新日期：2002-11-01 00:00:00

abstract：:We analyzed the cellular contents of not only major polyamines but also minor polyamines in slime molds Physarum polycephalum and Dictyostelium discoideum. The presence of putrescine and spermidine in either plasmodia or myxamoebae of these molds as major polyamines was confirmed. In addition to these polyamines, appr...

journal_title：Journal of biochemistry

authors： Hamana K,Matsuzaki S

更新日期：1984-04-01 00:00:00

abstract：:A cDNA clone inducible by either a tumor promoter, 12-o-tetradecanoyl phorbol-13-acetate (TPA), or a T-cell mitogen, phytohemagglutinin (PHA), was isolated from a cDNA library constructed from the poly(A) + RNAs of TPA- and PHA-stimulated human tonsillar lymphocytes, and was named pLD78. Stimulation of the tonsillar l...

journal_title：Journal of biochemistry

authors： Obaru K,Fukuda M,Maeda S,Shimada K

更新日期：1986-03-01 00:00:00

abstract：:Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

journal_title：Journal of biochemistry

authors： Morishima N,Ikai A

更新日期：1987-12-01 00:00:00

abstract：:We studied the effect of Ca2+ on the reactivities of two SH groups of DTNB light chain (Cys 128 and Cys 157) using a fluorogenic thiol reagent. It was found that a Ca2+-induced change in reactivity occurred only with Cys 128 when the light chain was in an isolated state, whereas it occurred with both Cys 128 and Cys 1...

journal_title：Journal of biochemistry

authors： Yamamoto K,Honjo R,Sekine T

更新日期：1980-01-01 00:00:00

abstract：:alpha-Actinin purified from chicken gizzard smooth muscle was characterized in comparison with alpha-actinins from chicken striated muscles, or fast-skeletal muscle, slow-skeletal muscle, and cardiac muscle. The gizzard alpha-actinin molecule consisted of two apparently identical subunits with a molecular weight of 10...

journal_title：Journal of biochemistry

authors： Endo T,Masaki T

更新日期：1982-11-01 00:00:00

abstract：:Protein kinases are essential mediators of cellular signal transduction and are often dysregulated in disease. Among these, protein tyrosine kinases (PTKs) have received specific interest due to their common roles in various diseases including cancer, and emerging observations indicating that PTK signalling pathways a...

journal_title：Journal of biochemistry

authors： Dustin CM,Heppner DE,Lin MJ,van der Vliet A

更新日期：2020-02-01 00:00:00

abstract：:The cDNAs encoding the precursors of cystatin SN, cystatin S, and two mutants of cystatin S (-18R-->W; 117R-->W) were expressed in Escherichia coli JM109 with isopropyl-beta-D-thio-galactoside (IPTG) induction. Premature cystatin S with the original signal [-20MARPLCTLLLLMATLAGALA] was processed and a large amount of ...

journal_title：Journal of biochemistry

authors： Saitoh E,Isemura S

更新日期：1994-08-01 00:00:00

abstract：:Two-dimensional gel electrophoresis using 0.5% agarose gel in the 1st dimension and 2-12% gradient polyacrylamide gel in the 2nd dimension succeeded in the isoelectrical focusing of connectin, a giant myofibrillar protein of approximately 3,000 kDa. Immunoblotting with an anti-connectin monoclonal antibody, SM1-36-2, ...

journal_title：Journal of biochemistry

authors： Matsumura K,Hori S,Sugiura H,Shimizu T

更新日期：1990-10-01 00:00:00

abstract：:We tested the capsid targeted viral inactivation method as an anti-HBV strategy. HepG2 cells were cotransfected with HBV expression plasmid and the plasmid encoding fusion protein of either Core-A3C or Core-humanized renilla GFP (hrGFP). Core-A3C had substantial effect on HBV DNA levels. In the HepG2 cells expressing ...

journal_title：Journal of biochemistry

authors： Li D,Liu J,Kang F,Guan W,Gao X,Wang Y,Sun D

更新日期：2011-10-01 00:00:00

abstract：:We developed a method to quantify galectin-7 extracted from adult mouse tissues by Western blot analysis. More than 0.5 ng of galectin-7 per mg of tissue was detectable by this method. The amounts of galectin-7 in tissues were determined as follows: skin, 62 +/- 3 ng/mg; esophagus, 23 +/- 8 ng/mg; stomach, 18 +/- 6 ng...

journal_title：Journal of biochemistry

authors： Sato M,Nishi N,Shoji H,Kumagai M,Imaizumi T,Hata Y,Hirashima M,Suzuki S,Nakamura T

更新日期：2002-02-01 00:00:00

abstract：:Catalase from Aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. The enzyme had a molecular weight of 385,000 as estimated from sedimentation measurements. Carbohydrate analyses showed that the catalase was a glycoprotein containing a...

journal_title：Journal of biochemistry

authors： Kikuchi-Torii K,Hayashi S,Nakamoto H,Nakamura S

更新日期：1982-11-01 00:00:00

abstract：:Several kinds of hydrophilic proteins were examined to determine their interaction with artificial liposomes. Mitochondrial aspartate aminotransferase (m-GOT) [EC 2.6.1.1], as well as cytochrome c, was found to interact strongly with negatively charged liposomes. In each case, an appreciable amount of the protein boun...

journal_title：Journal of biochemistry

authors： Furuya E,Yoshida Y,Tagawa K

更新日期：1979-05-01 00:00:00

abstract：:Cation specificity and effects of pH and temperature on membrane aggregation of porcine intestinal brush borders were studied in terms of the turbidity change of the membrane suspensions due to the addition of polyvalent cations. Monovalent cations (K+ and Na+) did not induce the membrane aggregation at 5 mM or below....

journal_title：Journal of biochemistry

authors： Ohyashiki T,Takeuchi M,Mohri T

更新日期：1984-03-01 00:00:00

abstract：:Human matrix metalloproteinase 7 (MMP-7) activity exhibits broad bell-shaped pH profile with the acidic and alkaline pK(a) (pK(e1) and pK(e2)) values of about 4 and 10. The ionizable group for pK(e2) was assigned to Lys or Arg by thermodynamic analysis; however, no such residues are present in the active site. Hence, ...

journal_title：Journal of biochemistry

authors： Morishima A,Yasukawa K,Inouye K

更新日期：2012-05-01 00:00:00

abstract：:The Escherichia coli phenylalanine-sensitive DAHP (3-deoxy-D-arabino-heptulosonate 7-phosphate) synthetase (aroG product) is one of the DAHP synthetase isozymes that catalyze the first committed step in the biosynthesis of aromatic amino acids and vitamins. Through target-directed mutagenesis of the cloned aroG on a p...

journal_title：Journal of biochemistry

authors： Ger YM,Chen SL,Chiang HJ,Shiuan D

更新日期：1994-11-01 00:00:00

abstract：:Dimeric T. flavoviridis phospholipase A2 has been studied in terms of the interaction with essential Ca2+ by equilibrium gel filtration, ultraviolet difference spectroscopy, fluorescence measurements, and chemical modifications with p-bromophenacyl bromide. The subunit bound to Ca2+ with a 1:1 molar ratio and no coope...

journal_title：Journal of biochemistry

authors： Ohno M,Honda A,Tanaka S,Mohri N,Shieh TC,Kihara H

更新日期：1984-10-01 00:00:00

abstract：:To improve our understanding of the physiological roles of parvalbumins, PA-1 (pI 4.78) and PA-2 (pI 4.97) parvalbumins were prepared from bullfrog skeletal muscle and their calcium binding properties were examined in a medium of constant ionic strength (I = 0.106, pH 6.80, at 20 degrees C) containing various concentr...

journal_title：Journal of biochemistry

authors： Ogawa Y,Tanokura M

更新日期：1986-01-01 00:00:00

abstract：:Using a new cannulation method, which completely prevented interruption of circulation to brain tissue during surgical operations, we could prepare a functionally intact hemoglobin-free isolated perfused rat head in situ. The frequency distribution (8-25 Hz) and amplitude of a spontaneous electroencephalogram, EEG (50...

journal_title：Journal of biochemistry

authors： Inagaki M,Tamura M

更新日期：1993-06-01 00:00:00

abstract：:The crystal structure of a ternary complex of meso-2,3-butanediol dehydrogenase with NAD+ and a competitive inhibitor, mercaptoethanol, has been determined at 1.7 A resolution by means of molecular replacement and refined to a final R-factor of 0.194. The overall structure is similar to those of the other short chain ...

journal_title：Journal of biochemistry

authors： Otagiri M,Kurisu G,Ui S,Takusagawa Y,Ohkuma M,Kudo T,Kusunoki M

更新日期：2001-02-01 00:00:00

abstract：:The Genome of Bacillus subtilis 168 was used for cloning and engineering of large-sized DNAs. A mouse genomic DNA of approximately 120 kb was cloned into a locus of the B. subtilis genome by ordered assembly of 20- to 50-kb mouse DNA segments. Cloned mouse DNA, maintained stably, was engineered through B. subtilis tra...

journal_title：Journal of biochemistry

authors： Itaya M,Nagata T,Shiroishi T,Fujita K,Tsuge K

更新日期：2000-11-01 00:00:00

abstract：:Gastric cancer (GC) is one of the most common cancers in gastrointestinal malignant tumours. Long non-coding RNAs were widely reported to play a significant role in the regulation of occurrence or development of tumours. Bioinformatics analysis and a wide range of experiments were conducted to explore the expression s...

journal_title：Journal of biochemistry

authors： Xin H,Yan Z,Cao J

更新日期：2020-11-01 00:00:00

abstract：:In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

journal_title：Journal of biochemistry

authors： Egami F

更新日期：1975-06-01 00:00:00

abstract：:1-O-Alk-1'-enyl-2-O-acetyl-glycerophosphocholine (vinyl form of PAF) was found with PAF in perfused rat and guinea pig hearts. The main molecular species of the vinyl form of PAF, after separation by reverse phase HPLC, were identified as 1-O-hexadec-, -octadec-, and -octadecen-1'-enyl-2-O-acetyl-GPCs (16:0, 18:0, and...

journal_title：Journal of biochemistry

authors： Nakayama R,Saito K

更新日期：1989-04-01 00:00:00

abstract：:Two novel oligosaccharides with the sialyl-Le(a) structure were isolated from human milk using a monoclonal antibody, MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography, and structurally characterized...

journal_title：Journal of biochemistry

authors： Kitagawa H,Nakada H,Fukui S,Funakoshi I,Kawasaki T,Yamashina I,Tate S,Inagaki F

更新日期：1993-10-01 00:00:00

abstract：:We determined the complete amino acid sequence of a novel subtilisin inhibitor, SIL15, which had been isolated from the culture supernatant of Streptomyces bikiniensis and shown to be a member of the Streptomyces subtilisin inhibitor (SSI)-like (SIL) protein family, and then identified its reactive site. SIL15 is comp...

journal_title：Journal of biochemistry

authors： Terabe M,Kojima S,Taguchi S,Momose H,Miura K

更新日期：1995-03-01 00:00:00

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:RING finger protein 8 (RNF8) is an E3 ligase that is pivotal for DNA repair. However, the role of RNF8 in ulcerative colitis (UC) remains unclear. The aim of this study is to investigate the effect and the mechanism of RNF8 on UC model induced by trinitrobenzene sulfonic acid (TNBS) in mice. Lentiviruses overexpressin...

journal_title：Journal of biochemistry

authors： Zhu Y,Shi Y,Ke X,Xuan L,Ma Z

更新日期：2020-11-01 00:00:00