Effects of an increase in perfusion pressure and of the direction of flow on carbohydrate metabolism in the perfused rat liver.


:The effects of increases in perfusion pressure and direction of perfusion flow (anterograde and retrograde) on hepatic carbohydrate metabolism were examined in the isolated perfused rat liver. Changing the direction of flow from anterograde (portal to caval vein) to retrograde (caval to portal vein) increased the rates of glycogenolysis and gluconeogenesis from sorbitol, lactate, pyruvate, and dihydroxyacetone. The extent of stimulation of gluconeogenesis by norepinephrine was higher during anterograde perfusion while stimulation by glucagon was higher during retrograde perfusion. Since the inflowing perfusion pressure was higher in retrograde perfusion (3.8 mmHg) than during anterograde perfusion (2.2 mmHg), we examined the effect of elevation in perfusion pressure on hepatic metabolism. In anterograde (portal to caval vein) perfusion, increases in perfusion pressure above the basal level (2.2 mmHg) caused increases in rates of glycogenolysis and gluconeogenesis with maximum rates at 4 mmHg. The extent of stimulation of gluconeogenesis by norepinephrine decreased and that by glucagon increased during perfusion at elevated pressure. At the same perfusion pressure (4 mmHg), there were no differences in rates of glycogenolysis and gluconeogenesis and in the responses to hormones between anterogradely and retrogradely perfused livers. The omission of Ca2+ ions from the perfusate abolished the effects of retrograde perfusion and of the elevation of perfusion pressure on carbohydrate metabolism. An infusion of A23187 (30 nM) induced an increase in both glycogenolysis and gluconeogenesis with unchanged perfusion pressure. The results suggest that elevated perfusion pressure during retrograde perfusion stimulates hepatic carbohydrate metabolism via a Ca(2+)-dependent process.


J Biochem


Journal of biochemistry


Shiota M,Chinzei F,Moriyama M,Kimura K,Sugano T




Has Abstract


1995-05-01 00:00:00












  • Thiobacillus ferrooxidans cytochrome c oxidase: purification, and molecular and enzymatic features.

    abstract::Cytochrome c oxidase from Thiobacillus ferrooxidans was purified to homogeneity and some of its properties were studied. The oxidase was solubilized with n-octyl-beta-D-thioglucoside (OTG) under acidic conditions (pH 4.0) and purified by one step of ion-exchange chromatography with a CM-Toyopearl column. The absorptio...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kai M,Yano T,Tamegai H,Fukumori Y,Yamanaka T

    更新日期:1992-12-01 00:00:00

  • Origin and early evolution of transition element enzymes.

    abstract::In this paper we speculate on the origin and early evolution of transition element enzymes. Iron, molybdenum, and zinc, the most abundant transition elements in seawater, presumably complexed with compounds accumulated in the primeval sea in the course of chemical evolution forming compounds with subsequently evolved ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Egami F

    更新日期:1975-06-01 00:00:00

  • Lectin-dependent inhibition of antigen-antibody reaction: application for measuring α2,6-sialylated glycoforms of transferrin.

    abstract::We developed a high-throughput Enzyme-linked immunosorbent assay (ELISA) for measuring α2,6-sialylated transferrin (Tf), based on inhibition of anti-Tf antibody binding to α2,6-sialylated Tf by a lectin, Sambucus sieboldiana Agglutinin (SSA). The inhibition was not observed with other glycoforms, such as periodate-tre...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hoshi K,Kariya Y,Nara K,Ito H,Matsumoto K,Nagae M,Yamaguchi Y,Nakajima M,Miyajima M,Arai H,Kuno A,Narimatsu H,Shirotani K,Hashimoto Y

    更新日期:2013-09-01 00:00:00

  • Exploring the nucleotide-binding site in proteins by affinity labeling and site-directed mutagenesis.

    abstract::Affinity labeling with nucleoside polyphosphopyridoxals, especially those with 3 or 4 phosphate groups, was effective for identifying the lysyl residue(s) located at or near the binding site for ATP, GTP, UDP-Glc, or ADP-Glc in various proteins. Furthermore, kinetic analysis of the mutant enzymes, in which the labeled...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Fukui T

    更新日期:1995-06-01 00:00:00

  • Mitochondrial dynamics and interorganellar communication in the development and dysmorphism of mammalian oocytes.

    abstract::Mitochondria play many critical roles in cells, not only by supplying energy, but also by supplying metabolites, buffering Ca2+ levels and regulating apoptosis. During oocyte maturation and subsequent embryo development, mitochondria change their morphology by membrane fusion and fission, and coordinately undergo mult...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Udagawa O,Ishihara N

    更新日期:2020-03-01 00:00:00

  • Immunological cross-reactivity between large and small (2'-5')oligoadenylate synthetases from pig cells.

    abstract::Using glycerol gradient centrifugation, the molecular sizes of porcine (2'-5')oligoadenylate synthetases (2-5A synthetases) were estimated. The 2-5A synthetase purified from pig spleen was about 150 kDa, while the enzyme extracted from nuclei of Newcastle disease virus-infected pig epithelial cells (SK-h) was about 20...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Shimizu N,Sokawa Y,Sokawa J

    更新日期:1984-06-01 00:00:00

  • Ca2+- and S1-induced conformational changes of reconstituted skeletal muscle thin filaments observed by fluorescence energy transfer spectroscopy: structural evidence for three States of thin filament.

    abstract::Rabbit skeletal muscle alpha-tropomyosin (Tm) and the deletion mutant (D234Tm) in which internal actin-binding pseudo-repeats 2, 3, and 4 are missing [Landis et al. (1997) J. Biol. Chem. 272, 14051-14056] were used to investigate the interaction between actin and tropomyosin or actin and troponin (Tn) by means of fluo...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hai H,Sano K,Maeda K,Maéda Y,Miki M

    更新日期:2002-03-01 00:00:00

  • Occurrence of low molecular weight O-acetylserine sulfhydrylase in the yeast Saccharomyces cerevisiae.

    abstract::Studies with crude preparations obtained from a cysteine auxotroph of Saccharomyces cerevisiae showed that O-acetylserine sulfhydrylase could be separated from O-acetylhomoserine sulfhydrylase by chromatography on a DEAE-cellulose column and centrifugation in a sucrose density gradient. On the basis of sedimentation d...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yamagata S

    更新日期:1980-11-01 00:00:00

  • Existence of phosphoinositide-specific phospholipase C in rat liver nuclei and its change during liver regeneration.

    abstract::We found phosphoinositide-specific phospholipase C (PtdIns-PLC) activity in nuclei isolated from rat liver. The enzyme hydrolyzed phosphatidylinositol, phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol 4,5-bisphosphate in a Ca(2+)-dependent manner, and produced inositol mono-, bis-, and triphosphate,...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kuriki H,Tamiya-Koizumi K,Asano M,Yoshida S,Kojima K,Nimura Y

    更新日期:1992-03-01 00:00:00

  • Change in the expression of hypopharyngeal-gland proteins of the worker honeybees (Apis mellifera L.) with age and/or role.

    abstract::The roles of adult worker honeybees change with age; young workers nurse brood by secreting bee milk (royal jelly), and older workers forage for nectar and pollen and process nectar into honey. The electrophoretic profile of worker hypopharyngeal-gland proteins changes with age and/or role. Immunoblotting analysis usi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kubo T,Sasaki M,Nakamura J,Sasagawa H,Ohashi K,Takeuchi H,Natori S

    更新日期:1996-02-01 00:00:00

  • Rho family GTPases as key regulators for neuronal network formation.

    abstract::Rho family GTPases act as transducers of signals from extracellular stimuli to the cytoskeleton and gene expression. Their actions are temporal and spatial determinants for cellular functions. The cellular functions of Rho family GTPases have been studied in fibroblasts and endothelial cells, and recent advances have ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Negishi M,Katoh H

    更新日期:2002-08-01 00:00:00

  • Stable expression of gastric proton pump activity at the cell surface.

    abstract::Stable cell lines expressing the gastric proton pump alpha- and/or beta-subunits were constructed. The cell line co-expressing the alpha- and beta-subunits showed inward Rb(+) transport, which was activated by Rb(+) in a concentration-dependent manner. In the alpha+beta-expressing cell line, rapid recovery of intracel...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kimura T,Yoshida A,Tabuchi Y,Ikari A,Takeguchi N,Asano S

    更新日期:2002-06-01 00:00:00

  • Mitochondrial targeting signal-induced conformational change and repression of the peroxisomal targeting signal of the precursor for rat liver serine:pyruvate/alanine:glyoxylate aminotransferase.

    abstract::In the rat liver, two mRNAs for serine:pyruvate (or alanine:glyoxylate) aminotransferase are generated from a single gene by alternative transcription initiation. The longer mRNA encodes a precursor of a mitochondrial enzyme that has a mitochondrial targeting signal at the N-terminus and is translocated into mitochond...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oda T,Uchida C,Miura S

    更新日期:2000-04-01 00:00:00

  • Identification of the catalytic residues of sequence-specific and histidine-free ribonuclease colicin E5.

    abstract::Colicin E5 cleaves tRNAs for Tyr, His, Asn and Asp in their anticodons to abolish protein synthesis in Escherichia coli. We previously showed how its C-terminal RNase domain, E5-CRD, recognizes the anticodon bases but the catalytic mechanism remained to be elucidated. Although the reaction products with 5'-OH and 2',3...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Inoue-Ito S,Yajima S,Fushinobu S,Nakamura S,Ogawa T,Hidaka M,Masaki H

    更新日期:2012-10-01 00:00:00

  • beta-glycosidase from the hyperthermophilic archaeon Sulfolobus solfataricus: structure and activity in the presence of alcohols.

    abstract::beta-Glycosidase from the extreme thermophilic archaeon Sulfolobus solfataricus is a tetrameric protein with a molecular mass of 240 kDa, stable in the presence of detergents, and with a maximal activity at temperatures above 95 degrees C. Understanding the structure-activity relationships of the enzyme under differen...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: D'Auria S,Nucci R,Rossi M,Bertoli E,Tanfani F,Gryczynski I,Malak H,Lakowicz JR

    更新日期:1999-09-01 00:00:00

  • Binding of activated macrophages to tumor cells through a macrophage lectin and its role in macrophage tumoricidal activity.

    abstract::A 45-60 kDa Gal/GalNAc-specific macrophage lectin was found to participate in the interaction between tumor cells and tumoricidal macrophages activated by an antitumor streptococcal preparation, OK-432, and in the tumoricidal activity of the activated macrophages. The binding between OK-432-elicited activated macropha...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Oda S,Sato M,Toyoshima S,Osawa T

    更新日期:1989-06-01 00:00:00

  • Analysis of protein carbonyls with 2,4-dinitrophenyl hydrazine and its antibodies by immunoblot in two-dimensional gel electrophoresis.

    abstract::Protein carbonyls are reported to increase in aging and in pathologies such as Alzheimer's disease and ischemic injury. Detailed study of this important issue has, however, been hampered by lack of an appropriate method to identify individual carbonylated proteins. We describe here an immunoblot method to identify ind...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nakamura A,Goto S

    更新日期:1996-04-01 00:00:00

  • Phospholipid reverse micelles as a milieu of an enzyme reaction in an apolar system.

    abstract::Alkaline phosphatase was entrapped in reverse micelles formed in n-heptane with surfactants (bis(2-ethylhexyl)sodium sulfosuccinate, phosphatidylcholine, phosphatidylethanolamine, or phosphatidic acid) and water. The entrapped enzyme could express its activity in the reverse micelles of the above surfactants only when...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohshima A,Narita H,Kito M

    更新日期:1983-05-01 00:00:00

  • Analysis of the upstream regulatory region of the GTS1 gene required for its oscillatory expression.

    abstract::The protein level of the GTS1 gene product (Gts1p) fluctuated during the oscillation of energy metabolism in continuous culture of the yeast Saccharomyces cerevisiae. Here, we found that the GTS1 mRNA level oscillated with the same periodicity as the metabolic oscillation, suggesting that the expression of GTS1 was re...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tonozuka H,Wang J,Mitsui K,Saito T,Hamada Y,Tsurugi K

    更新日期:2001-11-01 00:00:00

  • Inhibition of palmitoyl CoA of EDTA- and Mg2+-ATPase of heavy meromyosin from rabbit skeletal muscle.

    abstract::Palmitoyl CoA inhibited EDTA-ATPase of heavy meromyosin (HMM) prepared from rabbit skeletal muscle. The concentration for half maximum inhibition of EDTA-ATPase was about 18 microM. Myristoyl CoA, the other long chain fatty acyl CoA, also inhibited EDTA-HMM ATPase, but CoA and short chain CoA thioesters, such as butyr...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujiwara A,Fujisaki H,Asai H,Yasumasu I

    更新日期:1981-09-01 00:00:00

  • Genomic organization of the human adipocyte-derived leucine aminopeptidase gene and its relationship to the placental leucine aminopeptidase/oxytocinase gene.

    abstract::The genomic organization of the gene encoding the human adipocyte-derived leucine aminopeptidase (A-LAP) has been determined. The gene is composed of 20 exons and 19 introns and spans approximately 47 kilobases of chromosome 5q15. The gluzincin aminopeptidase motif, the HEXXH(X)(18)E zinc-binding motif essential for e...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hattori A,Matsumoto K,Mizutani S,Tsujimoto M

    更新日期:2001-08-01 00:00:00

  • Restoration of Ca2+-transport in sarcoplasmic reticulum ATPase solubilized with octaethyleneglycol mono n-dodecyl ether.

    abstract::One mg protein/ml of sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle were solubilized with 50 mg/ml of octaethyleneglycol mono n-dodecyl ether (C12E8) in a solution containing 5 mM CaCl2, 0.1 M KCl, and 20% glycerol at pH 7.5. When 30 mg/ml of soybean lecithin was added to this mixture and t...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Andoh R,Yamamoto T

    更新日期:1985-03-01 00:00:00

  • Isolation of a 5S RNA-protein L5 complex from 60S subunits of rat liver ribosomes by cesium sulfate density-gradient equilibrium centrifugation.

    abstract::Upon CS2SO4 density-gradient equilibrium centrifugation, 60S subunits of rat liver ribosomes were dissociated to form three bands at the densities of 1.55, 1.40, and 1.30 g/ml. The bands at 1.55 and 1.30 g/ml were shown to contain exclusively RNA or proteins, respectively, whereas the band at 1.40 g/ml contained both ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Isoda N,Tanaka T,Ishikawa K

    更新日期:1981-08-01 00:00:00

  • Monoclonal antibodies recognizing amino-terminal and carboxy-terminal regions of human aldolase A: probes to detect conformational changes of the enzyme.

    abstract::Three monoclonal antibodies (MAbs1A2, 3C5, and 4C2) for human aldolase A [EC] were established. MAbs1A2, 3C5, and 4C2 were shown to belong to subclasses IgM, IgG1, and IgG2a, respectively. None of the MAbs inhibits aldolase A activity. Their epitopes were mapped in detail on the molecule by examining the reac...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kitajima Y,Matsuhashi S,Nishida H,Takasaki Y,Takahashi I,Hisatsugu T,Hori K

    更新日期:1991-04-01 00:00:00

  • Real time observation of binding of herpes simplex virus type 1 (HSV-1) to Vero cells and neutralization of HSV-1 by sulfonated human immunoglobulin.

    abstract::A real time biomolecular interaction assay system involving an optical sensor was applied to the quantitative analysis of the binding of herpes simplex virus type 1 (HSV-1) to Vero cells, and the neutralization antibody titer against this virus with a commercially available sulfonated human immunoglobulin preparation....

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Inoue K,Arai T,Aoyagi M

    更新日期:1997-04-01 00:00:00

  • Characterization of the human E2F4 promoter region and its response to 12-O-tetradecanoylphorbol-13-acetate.

    abstract::The E2F transcription factors (TFs), which control the progression of the cell cycle in response to DNA-damage and various stresses, are known to interact with a tumor suppressor, Retinoblastoma 1 (RB1). We previously showed that the response of the human RB1 promoter to a 12-O-tetradecanoylphorbol-13-acetate (TPA) in...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hamada H,Goto Y,Arakawa J,Murayama E,Ogawa Y,Konno M,Oyama T,Asai M,Sato A,Tanuma SI,Uchiumi F

    更新日期:2019-06-14 00:00:00

  • Binding of intrinsic ATPase inhibitor to mitochondrial ATPase--stoichiometry of binding of nucleotides, inhibitor, and enzyme.

    abstract::F1-ATPase inhibitor was purified from yeast, Saccharomyces cerevisiae. The purified inhibitor blocked ATPase activity in the presence of ATP and Mg2+ by forming a latent equimolar enzyme-inhibitor complex with ATP and ADP newly bound to loose sites on the enzyme. A small portion of externally added ATP was hydrolyzed ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Hashimoto T,Negawa Y,Tagawa K

    更新日期:1981-10-01 00:00:00

  • Isolation and characterization of lectins specific for mannose/fucose/N-acetylglucosamine from rat peritoneal macrophages.

    abstract::Rat peritoneal macrophages were shown to have two distinct mannose/fucose/N-acetylglucosamine-specific lectins. The major lectin of 180 kDa, which is similar in size to the mannose receptor first isolated from alveolar macrophages (Wileman, T.E., Lennartz, M.R., & Stahl, P.D. (1986) Proc. Natl. Acad. Sci. U.S. 83, 250...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ii M,Wada M,Kawasaki T,Yamashina I

    更新日期:1988-10-01 00:00:00

  • Evolutionary relationship of denitrifying bacteria as deduced from 5S rRNA sequences.

    abstract::The nucleotide sequences of 5S rRNA from seven denitrifying bacteria have been determined. Based on these sequences and those reported in the literature (including two denitrifiers), a phylogenic tree of 104 eubacterial 5S rRNA sequences has been constructed to establish the position of the denitrifying bacteria. Thes...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohkubo S,Iwasaki H,Hori H,Osawa S

    更新日期:1986-11-01 00:00:00

  • Identification of novel members of the Xenopus Ca2+ -dependent lectin family and analysis of their gene expression during tail regeneration and development.

    abstract::We previously demonstrated that the gene for a member of the humoral C-type lectin family is transiently expressed in the regenerating legs of the American cockroach [Arai et al., Insect Biochem. Mol. Biol. 28, 987-994 (1998)]. To identify candidate lectin(s) involved in tail regeneration in the Xenopus laevis tadpole...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ishino T,Kunieda T,Natori S,Sekimizu K,Kubo T

    更新日期:2007-04-01 00:00:00