Abstract:
:The ferric uptake regulator A (FurA) plays an essential role in responding to oxidative stress in mycobacteria. The genome of Mycobacterium smegmatis harbours three FurA orthologs; however, the potential cross-talk and contribution to drug resistance of different furA operon remain underdetermined. In this study, we characterized the cross-regulation and effect in drug resistance of these orthologs from M. smegmatis. Cross-binding of FurA protein to furA promoter was observed. The binding of FurA1 to furA3p and FurA2 to furA1p or furA3p is even more pronounced than their self-binding. The three FurA proteins are all functional at repressing the expression of the peroxidase enzyme katG1/katG2 in vivo. When overexpressing any of the furA orthologs in M. smegmatis, the bacteria become more resistant to isoniazid (INH). This pattern is consistent with that in Mycobacterium bovis. However, the knockdown of furA does not affect the INH sensitivity. This is the first report of cross-talk and contribution to drug resistance of all three furA orthologs in M. smegmatis.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Gao CH,Wei WP,Tao HL,Cai LK,Jia WZ,Hu L,Yang Mdoi
10.1093/jb/mvz030subject
Has Abstractpub_date
2019-09-01 00:00:00pages
237-243issue
3eissn
0021-924Xissn
1756-2651pii
5472330journal_volume
166pub_type
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doi:10.1093/oxfordjournals.jbchem.a121740
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1983-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
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更新日期:1989-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132985
更新日期:1980-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123267
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pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1976-05-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135367
更新日期:1985-10-01 00:00:00
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更新日期:1984-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-01-01 00:00:00
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更新日期:1996-05-01 00:00:00
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更新日期:2021-01-22 00:00:00
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更新日期:2001-12-01 00:00:00