Crystallization and preliminary X-ray analysis of a DNA primase from hyperthermophilic archaeon Pyrococcus horikoshii.

abstract：:The process of mitochondrial dysfunction in ischemic rat liver was studied. A close correlation was found between decrease in the mitochondrial adenine nucleotide content and deterioration of oxidative phosphorylation capacity. The level of total adenine nucleotides, which was 15--20 nmol/mg protein in mitochondria is...

journal_title：Journal of biochemistry

authors： Watanabe F,Kamiike W,Nishimura T,Hashimoto T,Tagawa K

更新日期：1983-08-01 00:00:00

abstract：:Peptide:N-glycanase (PNGase) is the enzyme responsible for de-N-glycosylation of misfolded glycoproteins in the cytosol. Here, we report the molecular identification and characterization of PNGase (png-1, F56G4.5) from Caenorhabditis elegans. This enzyme released both high mannose- and complex-type N-glycans from glyc...

journal_title：Journal of biochemistry

authors： Kato T,Kawahara A,Ashida H,Yamamoto K

更新日期：2007-08-01 00:00:00

abstract：:In porcine aortic smooth muscle cells, neuropeptide Y (NPY) stimulates mobilization of CA(2+) from intracellular store sites via Y1 receptors. However, it has been debated whether or not Ca(2+) mobilization by Y1 receptors depends on the generation of inositol 1,4,5-trisphosphate [Ins(1,4,5)P3] following activation of...

journal_title：Journal of biochemistry

authors： Shigeri Y,Nakajima S,Fujimoto M

更新日期：1995-09-01 00:00:00

abstract：:Three kinds of phospholipase C [EC 3.1.4.3] were used to selectively hydrolyze phospholipids in rat liver microsomes, and their effects on the acyl-CoA: glycerophosphate and acyl-CoA: lysophospholipids acyltransferase systems were examined. The glycerophosphate acyltransferase [EC 2.3.1.15] system was inactivated rapi...

journal_title：Journal of biochemistry

authors： Sonoki S,Ikezawa H

更新日期：1976-12-01 00:00:00

abstract：:Comparative studies of the unfolding equilibria of two homologous proteins, bovine alpha-lactalbumin and hen lysozyme, induced by treatment with guanidine hydrochloride have been made by analysis of the peptide and the aromatic circular dichroism spectra. The effect of the specific binding of Ca2+ ion by the former pr...

journal_title：Journal of biochemistry

authors： Ikeguchi M,Kuwajima K,Sugai S

更新日期：1986-04-01 00:00:00

abstract：:The substrate specificity of microbial transglutaminase (MTG) from Streptomyces mobaraensis (formerly categorized Streptoverticillium) was studied using a Streptomyces proteinaceous protease inhibitor, STI2, as a model amine-donor substrate. Chemical modification and mutational analysis to address the substrate requir...

journal_title：Journal of biochemistry

authors： Taguchi S,Nishihama KI,Igi K,Ito K,Taira H,Motoki M,Momose H

更新日期：2000-09-01 00:00:00

abstract：:Murine interleukin-2 (MIL-2) cDNA deleted of 11 repeats of a CAG sequence, and that encoded from 33Met to 149Gln were inserted into an expression vector carrying an Escherichia coli tryptophan promoter and were expressed in E. coli, respectively. Recombinant MIL-2 deleted of 11 glutamine repeats (MIL-2(-Gln] supported...

journal_title：Journal of biochemistry

authors： Kashima N,Matsui H,Yamasaki S,Kondo N,Hamuro J

更新日期：1987-10-01 00:00:00

abstract：:Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the reversed arrangement of the variable domains of the heavy and light chains ...

journal_title：Journal of biochemistry

authors： Merk H,Stiege W,Tsumoto K,Kumagai I,Erdmann VA

更新日期：1999-02-01 00:00:00

abstract：:Triacylglycerol lipase activities in adrenals and testes of rats were measured and the properties of the enzymes were characterized. The enzymes were extracted from acetone-ether dried powder of the tissues and the crude extracts were applied to a heparin-Sepharose affinity column. The enzymes bound to the column were...

journal_title：Journal of biochemistry

authors： Murase T,Yamada N,Akanuma Y,Ohsawa N

更新日期：1982-09-01 00:00:00

abstract：:The dephosphorylation of phospho-amino acids with alkaline phosphatase (AlPase) from calf intestine or Escherichia coli and the phosphorylation of bovine serum albumin (BSA) with epidermal growth factor (EGF) receptor kinase from human A431 epidermoid carcinoma cells were investigated by 31P NMR spectroscopy. The init...

journal_title：Journal of biochemistry

authors： Takahashi K,Shimidzu M,Shindo H,Kawamoto T,Nishi M,Matsumoto U,Taniguchi S

更新日期：1987-05-01 00:00:00

abstract：:A cytochrome o-type oxidase from the thermophilic bacterium PS3 grown under air-limited conditions was purified by ion-exchange chromatography in the presence of a non-ionic detergent. The enzyme was composed of three subunits (60, 30, and 16 kDa) and seemed to contain two molecules of heme b as prosthetic groups. It ...

journal_title：Journal of biochemistry

authors： Sone N,Kutoh E,Sato K

更新日期：1990-04-01 00:00:00

abstract：:In order to examine the possibility of the use of a surface plasmon resonance (SPR) sensor for real-time monitoring of the process of refolding of immobilized proteins, the refolding of firefly luciferase immobilized on a carboxymethyldextran matrix layer was analyzed. The SPR signal of the immobilized luciferase decr...

journal_title：Journal of biochemistry

authors： Zako T,Harada K,Mannen T,Yamaguchi S,Kitayama A,Ueda H,Nagamune T

更新日期：2001-01-01 00:00:00

abstract：:To enhance the heat stability of the CutA1 protein from Escherichia coli (EcCutA1) so that it has comparable stability to CutA1 from Pyrococcus horikoshii with a denaturation temperature (T(d)) of 150°C, we used the Stability Profile of Mutant Protein (SPMP) to examine the structure-sequence (3D-1D) compatibility betw...

journal_title：Journal of biochemistry

authors： Matsuura Y,Ota M,Tanaka T,Takehira M,Ogasahara K,Bagautdinov B,Kunishima N,Yutani K

更新日期：2010-10-01 00:00:00

abstract：:We cloned and sequenced the Serratia marcescens prolyl aminopeptidase (SPAP) gene. Nucleotide sequence analysis revealed an open reading frame of 951 bp, encoding a protein of 317 amino acids with a predicted molecular weight of 36,083. The expressed enzyme was purified about 90-fold on columns of Toyopearl HW65C and ...

journal_title：Journal of biochemistry

authors： Kabashima T,Kitazono A,Kitano A,Ito K,Yoshimoto T

更新日期：1997-09-01 00:00:00

abstract：:The mechanism by which GTP induces Ca2+ release from Ca2(+)-preloaded rat hepatic microsomes was studied. In the same concentration range as that for Ca2+ release, GTP inhibited the initial rate of ATP-driven Ca2+ uptake. It also inhibited the formation by ATP of the phosphorylated intermediate of Ca2(+)-ATPase, which...

journal_title：Journal of biochemistry

authors： Kimura S,Higham S,Robison BC,Kraus-Friedmann N

更新日期：1990-04-01 00:00:00

abstract：:We isolated and characterized an anti-idiotype monoclonal antibody (AR42.1) which is capable of mimicking a distinct and specific epitope of MUC-1 antigen. The cDNA sequences coding for the AR42.1 variable regions were determined. We found significant amino acid homology between complementary determining regions 3 (CD...

journal_title：Journal of biochemistry

authors： Luo D,Qi W,Ma J,Wang YJ,Wishart D

更新日期：2000-09-01 00:00:00

abstract：:Serum-free culture medium collected from primary monolayer cultures of human articular chondrocytes was found to inhibit human urokinase [EC 3.4.21.31] activity. Although chondrocyte culture medium contained a small amount of endothelial-type plasminogen activator inhibitor which could be demonstrated by reverse fibri...

journal_title：Journal of biochemistry

authors： Yamada H,Stephens RW,Nakagawa T,McNicol D

更新日期：1988-12-01 00:00:00

abstract：:Partition of merocyanine dyes, which have a negative charge, onto photosynthetic membranes of chloroplasts and bacteria was analyzed by measuring the fluorescence intensity change, absorbance change, and amount of dye in the supernatant after centrifugation. The partition depended on the surface potential, which is a ...

journal_title：Journal of biochemistry

authors： Masamoto K,Matsuura K,Itoh S,Nishimura M

更新日期：1981-02-01 00:00:00

abstract：:Cholesterol: oxygen oxidoreductase [EC 1.1.3.6] from Brevibacterium sterolicum (ATCC 21387) was found to catalyze the oxidation of steroids such as sterols, steroid hormones, and bile acids having a free C-3beta hydroxyl group. However, the enzyme was inactive towards estradiol and estriol and had a weak activity towa...

journal_title：Journal of biochemistry

authors： Ikawa S,Takita M,Ogura M

更新日期：1979-06-01 00:00:00

abstract：:The melanocortin receptor type 4 (MC4-R) is involved in food intake and represents a potential target for the treatment of some forms of obesity. The fluorescent protein EGFP was fused to the wild-type or mutated coding sequence of the human MC4-R. After transfection in HEK 293, clones stably expressing hMC4-R-EGFP we...

journal_title：Journal of biochemistry

authors： Blondet A,Doghman M,Rached M,Durand P,Bégeot M,Naville D

更新日期：2004-04-01 00:00:00

abstract：:Dimeric T. flavoviridis phospholipase A2 has been studied in terms of the interaction with essential Ca2+ by equilibrium gel filtration, ultraviolet difference spectroscopy, fluorescence measurements, and chemical modifications with p-bromophenacyl bromide. The subunit bound to Ca2+ with a 1:1 molar ratio and no coope...

journal_title：Journal of biochemistry

authors： Ohno M,Honda A,Tanaka S,Mohri N,Shieh TC,Kihara H

更新日期：1984-10-01 00:00:00

abstract：:OS-9 gene is frequently coamplified with CDK4 gene in human sarcomas. We isolated and characterized three isoforms of OS-9 cDNA found in a myeloid leukemia HL-60 cDNA library. Isoform 1 consisted of 2,700 bp, from which a 667 amino acid sequence was deduced and found to be identical with that of OS-9 cDNA from osteosa...

journal_title：Journal of biochemistry

authors： Kimura Y,Nakazawa M,Yamada M

更新日期：1998-05-01 00:00:00

abstract：:Resonance Raman (RR) spectra were obtained in H2O or D2O solution for the purple intermediates of D-amino acid oxidase (DAO) with isotopically labeled substrates, i.e., [1-13C]-, [2-13C]-, [3-13C]-, [15N]-, and [3,3,3-D3]alanine; [carboxyl-13C]- and [15N]proline. RR spectra were also measured for the intermediates of ...

journal_title：Journal of biochemistry

authors： Nishina Y,Shiga K,Miura R,Tojo H,Ohta M,Miyake Y,Yamano T,Watari H

更新日期：1983-12-01 00:00:00

abstract：:Effects of anion transport inhibitors on hemolysis of human erythrocytes at 200 mPa were examined. The degree of hemolysis was decreased by treating intact cells with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonate and pyridoxal 5'-phosphate (PLP) had little effect on the hem...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Matsumoto M,Kimoto E

更新日期：1995-10-01 00:00:00

abstract：:Protein phosphorylation is a key regulatory mechanism of the organization and dynamics of the actin cytoskeleton during cell motility, differentiation, and cytokinesis. The level of protein phosphorylation is dependent on the relative activities of both protein kinases and protein phosphatases. In this paper, we exami...

journal_title：Journal of biochemistry

authors： Usui T,Marriott G,Inagaki M,Swarup G,Osada H

更新日期：1999-05-01 00:00:00

abstract：:In sea urchin embryos, primary mesenchyme cells, descendants from micromeres produced at the 16-cell stage, form spicules or CaCO3 deposits in their skeletal vacuoles, at the post-gastrula stage. Micromeres isolated at the 16-cell stage also differentiate into spicule-forming cells during their culture at the same tim...

journal_title：Journal of biochemistry

authors： Mitsunaga K,Fujino Y,Yasumasu I

更新日期：1986-12-01 00:00:00

abstract：:Raman spectroscopy was used to investigate the hydrogen bonding at the C(4)=O moiety of the isoalloxazine nucleus in a series of flavins and flavoproteins. Isotope effects of Raman bands confirmed that the band observed around 1,710 cm(-1) is mainly derived from C(4)=O stretching vibrational mode. A linear correlation...

journal_title：Journal of biochemistry

authors： Hazekawa I,Nishina Y,Sato K,Shichiri M,Miura R,Shiga K

更新日期：1997-06-01 00:00:00

abstract：:To study the effect of cross-linking of membrane proteins on vesiculation of human erythrocytes by dimyristoylphosphatidylcholine (DMPC), red cells were treated with diamide at atmospheric pressure or 100 MPa and then incubated with DMPC in buffers of pH 6.5-8.5. Irrespective of buffer pH, the amount of released vesic...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Yamada S,Kimoto E

更新日期：1994-04-01 00:00:00

abstract：:Leukotriene B4 (LTB4) is a potent inflammatory mediator derived from arachidonic acid. Two G protein-coupled receptors for LTB4 have been identified: a high-affinity receptor, BLT1, and a low-affinity receptor, BLT2. Both receptors mainly couple to pertussis toxin-sensitive Gi-like G proteins and induce cell migration...

journal_title：Journal of biochemistry

authors： Yokomizo T

更新日期：2015-02-01 00:00:00

abstract：:We generated and characterized novel antibodies specific for a cleavage site of human caspase-8/FLICE and its substrate, FLICE-like inhibitory protein (FLIP). The synthetic peptides used as immunogens were CQGDNYQKGIPVETD (#791) and VSEGQLEDSSLLEVD (#1342), which corresponded to cleaved regions of N-terminal fragments...

journal_title：Journal of biochemistry

authors： Niikura Y,Nonaka T,Imajoh-Ohmi S

更新日期：2002-07-01 00:00:00