Sulfation of chondroitin sulfate secreted by baby hamster kidney cells and their polyoma virus-transformed counterparts.

abstract：:Cucumisin was isolated from prince melon sarcocarp by means of a simple purification procedure. Serine protease inhibitors such as soybean trypsin inhibitor, ovomucoid, and aprotinin had no effect on the enzyme activity. alpha 2-Macroglobulin showed 38% inhibition of the original caseinolytic activity of cucumisin. Th...

journal_title：Journal of biochemistry

authors： Uchikoba T,Yonezawa H,Kaneda M

更新日期：1995-05-01 00:00:00

abstract：:A membrane protein insoluble in water was isolated by gel chromatography in the presence of 0.1% sodium dodecyl sulfate (SDS) from chromatophores of a photosynthetic bacterium, Rhodospirillum rubrum. This is one of the major membrane proteins of the chromatophore. The protein was found to bind about four grams of SDS ...

journal_title：Journal of biochemistry

authors： Miyake J,Ochiai-Yanagi S,Kasumi T,Takagi T

更新日期：1978-06-01 00:00:00

abstract：:The conformation of the chondroitin-4-sulfate calcium salt was investigated by X-ray analysis. The following results were obtained. 1, The repeat length per disaccharide was 0.913 nm: 2, The molecular chain had three-fold screw symmetry: 3, The shape of the unit cell was a trigonal prism with dimensions a=b=1.28 nm, c...

journal_title：Journal of biochemistry

authors： Tanaka K

更新日期：1978-01-01 00:00:00

abstract：:The complete amino acid sequence of Fragment B obtained by the limited tryptic digestion of E. coli polypeptide chain elongation factor Tu (EF-Tu) was determined. Seven peptides formed from Fragment B by cleavage with cyanogen bromide (designated as CB1 to CB7 according to their order of alignment from N- to C-termini...

journal_title：Journal of biochemistry

authors： Nakamura S,Nakayama N,Takahashi K,Kaziro Y

更新日期：1982-03-01 00:00:00

abstract：:Studies were conducted to induce urinary excretion of retinol-binding protein (RBP) in the rabbit by long-term administration of cadmium, and to isolate and characterize rabbit RBP. Two rabbits were exposed to Cd at a dose of 0.8 and/or 1.5mg/kg of body weight by means of subcutaneous injections five times per week. O...

journal_title：Journal of biochemistry

authors： Muto Y,Nakanishi M,Shidoji Y

更新日期：1976-04-01 00:00:00

abstract：:The functional activities of myosin head are located in a 95 kilodalton (kDa) heavy chain which can be divided into three fragments of 23 kDa, 50 kDa, and 20 kDa. ATP hydrolysis sites were suggested to be located in the 23 kDa and 50 kDa fragments, and actin binding sites were in the 50 kDa and 20 kDa fragments. In th...

journal_title：Journal of biochemistry

authors： Miyanishi T,Toyoshima C,Wakabayashi T,Matsuda G

更新日期：1988-03-01 00:00:00

abstract：:The conditions and pretreatments required in the conventional pyridine hemochrome method were re-examined for application of the method to the measurement of protoheme and heme c contents in photosynthetically grown cells of Rhodopseudomonas sphaeroides. The amounts of hemes were calculated from two kinds of differenc...

journal_title：Journal of biochemistry

authors： Takaichi S,Morita S

更新日期：1981-05-01 00:00:00

abstract：:Kinetic parameters, Km and Vmax for the transesterification of oligouridylic acid, (Up)nU greater than p (n=0-4), by RNase A were measured spectrophotometrically at pH 7.0 and 25 degrees C. The kinetic parameters, pKm and log Vmax increased with increase in the chain length (n), and seemed to be almost constant with s...

journal_title：Journal of biochemistry

authors： Irie M,Mikami F,Monma K,Ohgi K,Watanabe H,Yamaguchi R,Nagase H

更新日期：1984-07-01 00:00:00

abstract：:We detected a novel nuclear protein, MMBP-3, that bound to the c-Myc binding motif (CACGTG) in which deoxycytidine in the CpG sequence was methylated. MMBP-3 was partially purified by chromatography on heparin-agarose and hydroxyapatite, followed by affinity adsorption to a matrix coupled to the methylated binding mot...

journal_title：Journal of biochemistry

authors： Suetake I,Tajima S,Asano A

更新日期：1995-07-01 00:00:00

abstract：:Incubation of Sarcophaga fat bodies in buffered insect saline resulted in simultaneous activation of multiple defense protein genes [Sugiyama, H. and Natori, S. (1991) Eur. J. Biochem. 200, 495-500]. In this study, we demonstrated that the addition of both adenosine and homocysteine thiolactone to this medium abolishe...

journal_title：Journal of biochemistry

authors： Sugiyama H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:Picolinate binds to a reduced form of D-amino acid oxidase, and the complex formed has a broad absorption band around 600 nm as in the case of the purple intermediate of the enzyme with a substrate. The dissociation constant at 25 degrees C was 35 microns at pH 7.0. The pH dependence (pH 8.3-pH 6.4) of the dissociatio...

journal_title：Journal of biochemistry

authors： Nishina Y,Tojo H,Shiga K

更新日期：1986-03-01 00:00:00

abstract：:Aplyronine A is a macrolide isolated from Aplysia kurodai. By monitoring fluorescent intensity of pyrenyl-actin, it was found that aplyronine A inhibited both the velocity and the degree of actin polymerization. Aplyronine A also quickly depolymerized F-actin. The kinetics of depolymerization suggest that aplyronine A...

journal_title：Journal of biochemistry

authors： Saito S,Watabe S,Ozaki H,Kigoshi H,Yamada K,Fusetani N,Karaki H

更新日期：1996-09-01 00:00:00

abstract：:We have reported that Erbin expression was down-regulated in the Jurkat leukaemia T lymphocytes treated with the recombinant soluble tumour necrosis factor-related apoptosis-inducing ligand (rsTRAIL). Herein, we studied the expression and the regulation of Erbin and its binding partner, ErbB2, in the MCF-7 breast canc...

journal_title：Journal of biochemistry

authors： Liu N,Zhang J,Zhang J,Liu S,Liu Y,Zheng D

更新日期：2008-06-01 00:00:00

abstract：:Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...

journal_title：Journal of biochemistry

authors： Yamauchi T,Moroishi T

更新日期：2020-11-23 00:00:00

abstract：:X-ray diffraction photographs of a chicken gizzard G-actin.DNase I complex crystal have been recorded using the synchrotron radiation beam emitted by the Synchrotron Radiation Source at Daresbury and the Photon Factory at Tsukuba. The resolution limit was extended to 2.4 A and the exposure time was reduced approximate...

journal_title：Journal of biochemistry

authors： Sakabe N,Kamiya N,Sakabe K,Kondo H

更新日期：1984-03-01 00:00:00

abstract：:DL-[14C]Leucine or L-[3H]leucine was injected intraperitoneally into pyridoxine-deficient and control rats, and the subsequent incorporation of the radioactivities into aspartate aminotransferase (AspAT) isozymes and the total soluble protein in the liver was measured. AspAT in the cytosol (AspATc) was separated into ...

journal_title：Journal of biochemistry

authors： Shibuya M,Okada M

更新日期：1986-03-01 00:00:00

abstract：:We found that transducing phages carrying the gal or bio regions of the Escherichia coli genome were formed during in vitro packaging of endogenous lambda DNA. Structural analysis of the transducing phage genomes indicated that they were formed by abnormal excision of lambda prophage. Formation of transducing phages w...

journal_title：Journal of biochemistry

authors： Tomono M,Shiozaki M,Ikeda H

更新日期：1989-03-01 00:00:00

abstract：:The kinetics of the hydrogen-deuterium exchange reaction in sarcoplasmic reticulum (SR) membranes isolated from rabbit skeletal muscle was followed by infrared absorption measurements. The exchange rate in SR was much lower than that in the soluble proteins reported so far. When adenylyl-imidophosphate (AMPPNP, an TP ...

journal_title：Journal of biochemistry

authors： Anzai K,Kirino Y,Shimizu H

更新日期：1981-08-01 00:00:00

abstract：:The effects of a temperature shift-up and various metabolic inhibitors on the protein synthesis of an endosymbiont isolated from the pea aphid were studied. The syntheses of at least three major polypeptides were stimulated transiently immediately after a temperature shift-up, and treatment with ethanol and heavy meta...

journal_title：Journal of biochemistry

authors： Morioka M,Ishikawa H

更新日期：1992-04-01 00:00:00

abstract：:MF-18, one of the monoclonal antibodies generated to chicken myosin, cross-reacted with rabbit skeletal myosin subfragment-1 (S1). Utilizing an improved procedure of immuno-blotting, a decrease in reactivity of MF-18 to S1 by trinitrophenylation was observed. This indicates that the reactive lysyl residue is very clos...

journal_title：Journal of biochemistry

authors： Katayama E,Wakabayashi T,Reinach F,Masaki T,Fischman DA

更新日期：1984-03-01 00:00:00

abstract：:Bacteriochlorophyll (Bchl) protein complexes containing light harvesting (LH) Bchls were isolated from Chromatium vinosum, and their CD spectra were measured in the near-infrared region. These isolated Bchl protein complexes retained the CD signals of LH Bchls that were observed in situ in chromatophores. The CD spect...

journal_title：Journal of biochemistry

authors： Hayashi H,Nozawa T,Hatano M,Morita S

更新日期：1981-06-01 00:00:00

abstract：:The sensitivity and specificity of two influenza C virus assays, solid-phase and overlay assays, were investigated using naturally occurring 9-O-acetylated GD(3), rat serum glycoproteins containing 60% of N-acetyl-9-O-acetylneuraminic acid, and synthetically O-acetylated sialylated compounds. The sensitivity of the so...

journal_title：Journal of biochemistry

authors： Hubl U,Ishida H,Kiso M,Hasegawa A,Schauer R

更新日期：2000-06-01 00:00:00

abstract：:We developed a method to quantify galectin-7 extracted from adult mouse tissues by Western blot analysis. More than 0.5 ng of galectin-7 per mg of tissue was detectable by this method. The amounts of galectin-7 in tissues were determined as follows: skin, 62 +/- 3 ng/mg; esophagus, 23 +/- 8 ng/mg; stomach, 18 +/- 6 ng...

journal_title：Journal of biochemistry

authors： Sato M,Nishi N,Shoji H,Kumagai M,Imaizumi T,Hata Y,Hirashima M,Suzuki S,Nakamura T

更新日期：2002-02-01 00:00:00

abstract：:A simple and rapid method has been developed for the separation of apolipoproteins in high density lipoprotein (HDL) fractions by high performance liquid chromatography (HPLC) with gel permeation columns (G3000SW TSK GEL). The HPLC pattern monitored by A280 for a mixed solution of the HDL fraction (10 microliter) and ...

journal_title：Journal of biochemistry

authors： Kinoshita M,Okazaki M,Kato H,Teramoto T,Matsushima T,Naito C,Oka H,Hara I

更新日期：1983-08-01 00:00:00

abstract：:We describe cross-linking conditions for myosin subfragment-1 and actin with 1-ethyl-3-[3-(dimethylamino)propyl]carbodiimide, with which the compositions of cross-linked species can be altered. The specific Mg-ATPase activity of cross-linked subfragment-1 in these preparations was almost the same in spite of the diffe...

journal_title：Journal of biochemistry

authors： Yamamoto K

更新日期：1993-12-01 00:00:00

abstract：:Daunomycin, an anti-neoplastic agent, is known to be sequestered by acidic organelles in normal and multidrug-resistant cells [Willingham, M.C., Cornwell, M.M., Cardarelli, C.O., Gottesman, M.M., & Pastan, I. (1986) Cancer Res. 46, 5941-5946]. We studied the mechanism of accumulation of daunomycin into acidic organell...

journal_title：Journal of biochemistry

authors： Moriyama Y,Manabe T,Yoshimori T,Tashiro Y,Futai M

更新日期：1994-02-01 00:00:00

abstract：:We previously reported that internucleosomal DNA fragmentation, a biochemical feature of apoptosis, was induced spontaneously in high-cell-density culture of adult rat hepatocytes. To understand better the intracellular mechanism of the DNA fragmentation in this system, we have examined the effects of several inhibito...

journal_title：Journal of biochemistry

authors： Maeda S,Suzuki A,Lin KH,Inagaki H,Saito T

更新日期：1995-12-01 00:00:00

abstract：:We purified dipeptidyl peptidase II (DPP II) to homogeneity from rat kidney and determined its physicochemical properties, including its molecular weight, substrate specificity, and partial amino acid sequence. Furthermore, we screened a rat kidney cDNA library, isolated the DPP II cDNA and determined its structure. T...

journal_title：Journal of biochemistry

authors： Araki H,Li Y,Yamamoto Y,Haneda M,Nishi K,Kikkawa R,Ohkubo I

更新日期：2001-02-01 00:00:00

abstract：:The presence of a leucine zipper motif was recognized in the deduced amino acid sequences of human and rat renin-binding proteins (RnBPs) on cloning and sequence analysis of the RnBP cDNAs. The in vitro synthesized RnBPs, with the respective cDNAs, formed heterodimers with porcine renin and homodimers. On comparison o...

journal_title：Journal of biochemistry

authors： Inoue H,Takahashi S,Fukui K,Miyake Y

更新日期：1991-10-01 00:00:00

abstract：:The association constants of hen egg yolk riboflavin binding protein with 8-substituted riboflavins were measured using a fluorometric titration method in the range of 10-40 degrees C, and thermodynamic parameters were calculated using ordinary methods. The flavins used were riboflavins whose 8-methyl group was substi...

journal_title：Journal of biochemistry

authors： Matsui K,Sugimoto K,Kasai S

更新日期：1982-04-01 00:00:00