Abstract:
:The enthalpies of transfer of several amino acids and diglycine from water to aqueous polyols (glycerol, xylitol, sorbitol, and inositol) have been calorimetrically determined in order to clarify the mechanism of polyol-induced stabilization of proteins. The obtained enthalpy data were combined with the data on free energy of transfer previously determined to calculate the corresponding entropy of transfer. The enthalpies of transfer of nonpolar side chains of amino acids and peptide group were positive and negative, respectively, depending on the polyol concentration and hydrophobicity of the side chains. This indicates that the non-spontaneous transfer of nonpolar side chains to aqueous polyols is due to an enthalpy effect while that of peptide group is due to an entropy effect. These thermodynamic data are discussed in terms of changes in water structure or solvent ordering around the solute molecules. It was found that the polyol-induced stabilization of proteins is a result of a complicated enthalpy-entropy compensation phenomenon closely related to the solvent ordering around the solute molecules; consequently, the polyol effects on nonpolar side chains do not necessarily dominate those on peptide groups in an enthalpic sense.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Gekko Kdoi
10.1093/oxfordjournals.jbchem.a133639subject
Has Abstractpub_date
1981-12-01 00:00:00pages
1643-52issue
6eissn
0021-924Xissn
1756-2651journal_volume
90pub_type
杂志文章abstract::Guinea pig alveolar macrophages (A-M0) were studied with respect ot the phagocytic metabolism: the respiratory burst and its KCN sensitivity, the ability to generate H2O2, and myeloperoxidase (MPO) activity. Only fifty percent of the O2 uptake by A-M0 samples during phagocytosis was inhibited by the addition of 1 mK K...
journal_title:Journal of biochemistry
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doi:10.1093/oxfordjournals.jbchem.a133070
更新日期:1980-10-01 00:00:00
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更新日期:1979-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-05-01 00:00:00
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更新日期:2001-06-01 00:00:00
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pub_type: 杂志文章
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1975-02-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1988-03-01 00:00:00
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更新日期:2001-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1979-12-01 00:00:00
abstract::The nucleotide sequence at the intron-exon junction in the human beta-globin gene was analyzed by the quantification method (categorical discriminant analysis) proposed previously. Using the sample score of a 16-nucleotide sequence at a 3'-splice junction, we studied to what extent such a sequence contains the 3'-spli...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1990-12-01 00:00:00
abstract::Protein phosphorylation is a key regulatory mechanism of the organization and dynamics of the actin cytoskeleton during cell motility, differentiation, and cytokinesis. The level of protein phosphorylation is dependent on the relative activities of both protein kinases and protein phosphatases. In this paper, we exami...
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更新日期:1999-05-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:1991-10-01 00:00:00
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更新日期:1987-02-01 00:00:00
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更新日期:1983-10-01 00:00:00
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更新日期:1999-03-01 00:00:00
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更新日期:2001-03-01 00:00:00
abstract::To better understand the regulation of ryudocan (syndecan-4) expression, we have determined the structural organization of the human ryudocan gene. The human ryudocan gene extends approximately 24 kilobases and is divided into five exons, which appear to be conserved in syndecan family members. Exon I encodes the sign...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1996-05-01 00:00:00