Abstract:
:Pull-down assay and co-immunoprecipitation of cell extracts in which the integrase or reverse transcriptase of Moloney murine leukemia virus was transiently expressed showed that both enzymes interacted with PML proteins. In infected cells, interaction between the integrase and PML was also observed. Transient expression of PIASy and SUMO proteins facilitated SUMOylation of the integrase but had no apparent effects on the interaction with PML. A FLAG-tagged integrase co-localized with PML protein possibly in the PML body. Knockdown of PML by small interfering RNA resulted in reduced viral cDNA levels and integration efficiency. This suggested that PML proteins activated reverse transcription.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Okino Y,Inayoshi Y,Kojima Y,Kidani S,Kaneoka H,Honkawa A,Higuchi H,Nishijima K,Miyake K,Iijima Sdoi
10.1093/jb/mvs063subject
Has Abstractpub_date
2012-08-01 00:00:00pages
161-9issue
2eissn
0021-924Xissn
1756-2651pii
mvs063journal_volume
152pub_type
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