A simple and effective method for hemolysis with a hypoxanthine-xanthine oxidase system and alteration of erythrocyte phospholipid composition during the hemolysis.

abstract：:Glycoengineering of therapeutic proteins has been applied to improve the clinical efficacy of several therapeutics. Here, we examined the effect of glycosylation on the properties of the Fab of the therapeutic antibody, adalimumab. An N-glycosylation site was introduced at position 178 of the H-chain constant region o...

journal_title：Journal of biochemistry

authors： Nakamura H,Kiyoshi M,Anraku M,Hashii N,Oda-Ueda N,Ueda T,Ohkuri T

更新日期：2020-10-27 00:00:00

abstract：:The melanocortin receptor type 4 (MC4-R) is involved in food intake and represents a potential target for the treatment of some forms of obesity. The fluorescent protein EGFP was fused to the wild-type or mutated coding sequence of the human MC4-R. After transfection in HEK 293, clones stably expressing hMC4-R-EGFP we...

journal_title：Journal of biochemistry

authors： Blondet A,Doghman M,Rached M,Durand P,Bégeot M,Naville D

更新日期：2004-04-01 00:00:00

abstract：:The pH-activity curve of heavy meromyosin ATPase [EC 3.6.1.3] was measured at various temperatures. The pH-activity curve at higher temperatures showed a maximum at low pH and a minimum at pH 7 to 8 as has been already reported. At lower temperatures it was sigmoidal in shape, similar to a simple dissociation curve of...

journal_title：Journal of biochemistry

authors： Ishigami F,Morita F

更新日期：1977-02-01 00:00:00

abstract：:The major gangliosides were isolated from small intestine of 2-week-old mice of C3H/He strain and identified as GM3, GM1, and GD1a. These gangliosides characteristically contained a phytosphingosine moiety and alpha-hydroxy fatty acids. The gangliosides contained both N-glycolylneuraminic acid and N-acetylneuraminic a...

journal_title：Journal of biochemistry

authors： Sato E,Uezato T,Fujita M,Nishimura K

更新日期：1982-06-01 00:00:00

abstract：:In sea urchin embryos, primary mesenchyme cells, descendants from micromeres produced at the 16-cell stage, form spicules or CaCO3 deposits in their skeletal vacuoles, at the post-gastrula stage. Micromeres isolated at the 16-cell stage also differentiate into spicule-forming cells during their culture at the same tim...

journal_title：Journal of biochemistry

authors： Mitsunaga K,Fujino Y,Yasumasu I

更新日期：1986-12-01 00:00:00

abstract：:Human platelet membrane glycoprotein IV (GPIV), one of the major glycoprotein components, was purified by successive affinity chromatographies on columns of Lens culinaris agglutinin-Sepharose and wheat germ agglutinin-Sepharose followed by preparative polyacrylamide gel electrophoresis in the presence of sodium dodec...

journal_title：Journal of biochemistry

authors： Tsuji T,Osawa T

更新日期：1986-10-01 00:00:00

abstract：:A new multifunctional protein kinase, which normally exists as an inactive form in the soluble fraction in mammalian tissues, attaches to membranes to exhibit full enzymatic activity. A low concentration of Ca2+ is absolutely necessary for this activation. This process is reversible. cAMP shows no effect. The active f...

journal_title：Journal of biochemistry

authors： Takai Y,Kishimoto A,Iwasa Y,Kawahara Y,Mori T,Nishizuka Y,Tamura A,Fujii T

更新日期：1979-08-01 00:00:00

abstract：:Rho-kinase is a main player in the regulation of cytoskeletal events and a promising drug target in the treatment of both vascular and neurological disorders. Here we report the crystal structure of the Rho-kinase catalytic domain in complex with the specific inhibitor Y-27632. Comparison with the structure of PKA bou...

journal_title：Journal of biochemistry

authors： Yamaguchi H,Miwa Y,Kasa M,Kitano K,Amano M,Kaibuchi K,Hakoshima T

更新日期：2006-09-01 00:00:00

abstract：:In this study, we demonstrated that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a phosphatidylserine (PS)-binding protein and localized the putative PS-binding site involved in the membrane fusion induced by this enzyme. In an attempt to identify the PS-binding proteins, we raised polyclonal antibodies against...

journal_title：Journal of biochemistry

authors： Kaneda M,Takeuchi K,Inoue K,Umeda M

更新日期：1997-12-01 00:00:00

abstract：:Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each protein in different c...

journal_title：Journal of biochemistry

authors： Khademi F,Mostafaie A

更新日期：2010-05-01 00:00:00

abstract：:A simple, sensitive, and accurate method for determination of cholestanol in plasma is described. In this method a fixed amount of cholestane is added to 1 ml of plasma as an internal standard and steroids are saponified and extracted with n-hexane. The amounts of cholestanol and cholesterol are determined by mass fra...

journal_title：Journal of biochemistry

authors： Seyama Y,Ichikawa K,Yamakawa T

更新日期：1976-08-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:Dithiothreitol or glutathione, essential for amino acid incorporation into polypeptides in cell-free systems, inhibited glucosamine incorporation into ovalbumin, a glycoprotein containing about three residues of N-acetylucosamine and about five residues of mannose. However, the thiol was necessary for mannose incorpor...

journal_title：Journal of biochemistry

authors： Nakayama T,Narita K,Ogata K

更新日期：1976-05-01 00:00:00

abstract：:Undecaprenyl diphosphate synthase catalyzes the sequential condensation of eight molecules of isopentenyl diphosphate (IPP) in the cis-configuration into farnesyl diphosphate (FPP) to produce undecaprenyl diphosphate (UPP), which is indispensable for the biosynthesis of the bacterial cell wall. This cis-type prenyltra...

journal_title：Journal of biochemistry

authors： Kharel Y,Zhang YW,Fujihashi M,Miki K,Koyama T

更新日期：2003-12-01 00:00:00

abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...

journal_title：Journal of biochemistry

authors： Senshu T,Fukuda M,Ohashi M

更新日期：1978-10-01 00:00:00

abstract：:Translesion DNA synthesis (TLS) involves PCNA mono-ubiquitination and TLS DNA polymerases (pols). Recent evidence has shown that the mono-ubiquitination is induced not only by DNA damage but also by other factors that induce stalling of the DNA replication fork. We studied the effect of spontaneous DNA replication err...

journal_title：Journal of biochemistry

authors： Suzuki M,Niimi A,Limsirichaikul S,Tomida S,Miao Huang Q,Izuta S,Usukura J,Itoh Y,Hishida T,Akashi T,Nakagawa Y,Kikuchi A,Pavlov Y,Murate T,Takahashi T

更新日期：2009-07-01 00:00:00

abstract：:A high molecular weight protease inhibitor was purified from the egg white of Cuban crocodile (Crocodylus rhombifer). It inhibited the casein hydrolyzing activity of trypsin, subtilisin and papain. Its native molecular weight was 730,000 and it consisted of four subunits of equal molecular weight, each pair of which w...

journal_title：Journal of biochemistry

authors： Ikai A,Kitamoto T,Nishigai M

更新日期：1983-01-01 00:00:00

abstract：:Cell metabolism can be considered as a process of serial construction and destruction of cellular components, both of which must be regulated accurately. In eukaryotic cells, a variety of cellular components are actively delivered into lysosomes/vacuoles, specialized compartments for hydrolysis of macromolecules. Such...

journal_title：Journal of biochemistry

authors： Fujiwara Y,Wada K,Kabuta T

更新日期：2017-02-01 00:00:00

abstract：:Steady-state kinetic analyses suggest that Pseudomonas ochraceae 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (4-carboxy-2-hydroxy-cis,cis-muconate-6-semialdehyde: NADP+ oxidoreductase [EC 1.2.1.45]) functions through an ordered BiBi mechanism. The enzyme binds one NADP+ molecule per subunit with a Kd of 4...

journal_title：Journal of biochemistry

authors： Maruyama K

更新日期：1988-04-01 00:00:00

abstract：:Sulfation of glycosaminoglycans (GAGs) secreted by baby hamster kidney (BHK) cells and the polyoma virus-transformants (PY-BHK) was investigated. It has been reported that chondroitin sulfate (CS) of cell membranes from PY-BHK cells is undersulfated compared to that from BHK cells (Cancer Res. 43, 2712-2717, 1983). In...

journal_title：Journal of biochemistry

authors： Sugahara K,Fukui S,Yamashina I

更新日期：1985-10-01 00:00:00

abstract：:The gene encoding a MutM from Desulfovibrio vulgaris (Miyazaki F) was cloned and expressed in Escherichia coli. A 5.9-kb DNA fragment, isolated from D. vulgaris (Miyazaki F) by XhoI and PvuII, contained a MutM gene and other open reading frames. The nucleotide sequence of the MutM gene indicated that the protein was c...

journal_title：Journal of biochemistry

authors： Sanada H,Nakanishi T,Inoue H,Kitamura M

更新日期：2009-04-01 00:00:00

abstract：:The sigmaH of Bacillus subtilis directs transcription of a large number of early sporulation genes, whereas the principal sigma factor, sigmaA, is essential for the transcription of the genes for vegetative growth and early sporulation. We have purified sigmaA and sigmaH proteins, and characterized their properties. T...

journal_title：Journal of biochemistry

authors： Fujita M,Sadaie Y

更新日期：1998-07-01 00:00:00

abstract：:Wild mice are divided into two groups with different ganglioside compositions in the liver. Most Japanese and a few Chinese wild mice have GM2(NeuGc) as a major ganglioside, whereas all wild mice caught at other places distributed all over the world other than Japan and China express GM1(NeuGc) and GD1a(NeuGc) in addi...

journal_title：Journal of biochemistry

authors： Hashimoto Y,Suzuki A,Yamakawa T,Wang CH,Bonhomme F,Miyashita N,Moriwaki K

更新日期：1984-01-01 00:00:00

abstract：:Studies were performed in the rat liver to examine whether or not insulin as well as glucagon causes the induction of mitochondrial serine:pyruvate aminotransferase (SPTm) [EC 2.6.1.51] and if so, whether the mechanisms of induction are similar or different for the two hormones. Not only glucagon but also insulin indu...

journal_title：Journal of biochemistry

authors： Miyajima H,Oda T,Ichiyama A

更新日期：1989-04-01 00:00:00

abstract：:Four kinds of active sites of bacterial fatty acid synthetase were mapped on distinct regions within a subunit. Active sites were specifically labeled with radioactive substrates and active-site-directed inhibitors. Labeled enzymes were cleaved with proteases, and the fragments thus produced were identified with respe...

journal_title：Journal of biochemistry

authors： Morishima N,Ikai A

更新日期：1987-12-01 00:00:00

abstract：:In our previous study, the sphingosine-like immunosuppressant ISP-1 was shown to induce apoptosis in the mouse cytotoxic T cell line CTLL-2. In this study, we characterized the ISP-1-induced apoptotic pathway. Although caspase-3-like protease activity increases concomitantly with ISP-1-induced apoptosis in CTLL-2 cell...

journal_title：Journal of biochemistry

authors： Yamaji T,Nakamura S,Takematsu H,Kawasaki T,Kozutsumi Y

更新日期：2001-04-01 00:00:00

abstract：:At the initiation of chromosomal DNA replication, DNA primases synthesize short RNA primers, which are subsequently elongated by DNA polymerases. To understand the structural basis for the primer synthesis by archaeal/eukaryotic-type primases, the gene of the DNA primase from hyperthermophilic archaeon Pyrococcus hori...

journal_title：Journal of biochemistry

authors： Ito N,Nureki O,Shirouzu M,Yokoyama S,Hanaoka F

更新日期：2001-12-01 00:00:00

abstract：:The amount of S-II, a protein specifically stimulating RNA polymerase II, was measured in various mouse organs by a micro complement fixation assay. The amount was almost the same in brain, liver, kidney, spleen, and Ehrlich ascites tumor cells on the basis of DNA, being about 3.8 micrograms/mg DNA, which corresponds ...

journal_title：Journal of biochemistry

authors： Tamura H,Sekimizu K,Obinata M,Natori S

更新日期：1980-11-01 00:00:00

abstract：:Recombinant horse L-apoferritin and its mutants were used to compare the reactivities of two different cysteinyl residues with 7-fluoro-4-sulfamoyl-2,1,3-benzoxadiazole (ABD-F), p-chloromercuribenzoic acid (PCMB), N-(9-acridinyl)maleimide (NAM), and 4-maleimido-2,2,6,6-tetramethylpiperidine-N-oxyl (NEM-TEMPO). ABD-F s...

journal_title：Journal of biochemistry

authors： Takeda S,Yamaki M,Ebina S,Nagayama K

更新日期：1995-02-01 00:00:00

abstract：:The amino acid sequences of ferredoxins I and II from a blue-green alga, Nostoc strain MAC were determined. This alga is able to grow autotrophically in the light or heterotrophically in the dark. Analyses of tryptic peptides of Cm-proteins by conventional methods including solid-phase Edman degradation gave the compl...

journal_title：Journal of biochemistry

authors： Hase T,Matsubara H,Hutber GN,Rogers LJ

更新日期：1982-11-01 00:00:00