Abstract:
:Translesion DNA synthesis (TLS) involves PCNA mono-ubiquitination and TLS DNA polymerases (pols). Recent evidence has shown that the mono-ubiquitination is induced not only by DNA damage but also by other factors that induce stalling of the DNA replication fork. We studied the effect of spontaneous DNA replication errors on PCNA mono-ubiquitination and TLS induction. In the pol1L868F strain, which expressed an error-prone pol alpha, PCNA was spontaneously mono-ubiquitinated. Pol alpha L868F had a rate-limiting step at the extension from mismatched primer termini. Electron microscopic observation showed the accumulation of a single-stranded region at the DNA replication fork in yeast cells. For pol alpha errors, pol zeta participated in a generation of +1 frameshifts. Furthermore, in the pol1L868F strain, UV-induced mutations were lower than in the wild-type and a pol delta mutant strain (pol3-5DV), and deletion of the RAD30 gene (pol eta) suppressed this defect. These data suggest that nucleotide misincorporation by pol alpha induces exposure of single-stranded DNA, PCNA mono-ubiquitination and activates TLS pols.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Suzuki M,Niimi A,Limsirichaikul S,Tomida S,Miao Huang Q,Izuta S,Usukura J,Itoh Y,Hishida T,Akashi T,Nakagawa Y,Kikuchi A,Pavlov Y,Murate T,Takahashi Tdoi
10.1093/jb/mvp043subject
Has Abstractpub_date
2009-07-01 00:00:00pages
13-21issue
1eissn
0021-924Xissn
1756-2651pii
mvp043journal_volume
146pub_type
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