Abstract:
:The interactions of porcine alpha2-macroglobulin (alpha2M) with native proteinases, their zymogens and the chemically-modified enzymes were compared. The alpha2M did not bind to chymotrypsinogen, or to most of the chemically modified derivatives of alpha-chymotrypsin, trypsinogen, DIP- and PMS-trypsins, but it could interact with anhydrotrypsin, PMS-subtilisin, and O-acetylated neutral subtilopeptidase. Anhydrotrypsin appeared to bind very tightly to alpha2M, as does native trypsin, whereas the binding of PMS-subtilisin to alpha2M was weaker than that of the native enzyme, judging from exchange experiments with labeled enzyme and from competitive enzyme assay. There are, however, some differences in the mode of interaction with alpha2M between native and anhydrotrypsins. (1) The shape and the magnitude of ultraviolet difference spectra caused by the interaction with alpha2M were significantly different. (2) The interaction of alpha2M with active proteinase led to the formation of new amino-terminal amino acids, while that with anhydrotrypsin did not. (3) In vivo experiments showed that radioactivity of 3H-labeled trypsin-alpha2M complex was rapidly cleared from the plasma of rats, whereas the anhydrotrypsin-alpah2M complex was cleared very slowly. These results suggest that the proteolytic activity of the enzyme is not obligatory for the first phase of alpha2M-proteinase interaction (formation of Michaelis-type complex), but only the proteolytically modified complex is cleared rapidly from the blood circulation system.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Tsuru D,Kado K,Fujiwara K,Tmimatsu M,Ogita Kdoi
10.1093/oxfordjournals.jbchem.a132042subject
Has Abstractpub_date
1978-05-01 00:00:00pages
1345-53issue
5eissn
0021-924Xissn
1756-2651journal_volume
83pub_type
杂志文章abstract::Extracellular vesicles (EVs) are small particles that are naturally released from various types of cells. EVs contain a wide variety of cellular components, such as proteins, nucleic acids, lipids and metabolites, which facilitate intercellular communication in diverse biological processes. In the tumor microenvironme...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvaa132
更新日期:2020-11-23 00:00:00
abstract::The oxygen exchange during ATP hydrolysis by glycerinated muscle fibers, myofibrils, and synthetic actomyosin filaments was studied from the distribution of the [18O]Pi species produced by the hydrolysis of [gamma-18O]ATP. The products were mixtures of two species, one with a low extent of oxygen exchange and the othe...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122718
更新日期:1989-04-01 00:00:00
abstract::We previously reported that sera from various kinds of animals contain a protein(s) capable of inhibiting the growth of the non-malignant epithelial cell line derived from Buffalo rat liver (BRL). In the present study, a similar epithelial cell-specific growth inhibitor (EGI) was purified to homogeneity from an acid-e...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a121761
更新日期:1986-09-01 00:00:00
abstract::The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133204
更新日期:1981-01-01 00:00:00
abstract::1. 3'-Guanylyl-ethanol, 3'-guanylyl-propanol, and 3'-guanylyl-alpha-glycerol were synthesized by ribonuclease N1 [EC 3.1.4.8] using guanosine 2',3'-cyclic phosphate as a phosphate donor and various alcohols as phosphate acceptors. The yields of these phosphodiesters were 15%, 13.5%, 38.2%, respectively, with respect t...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131253
更新日期:1976-07-01 00:00:00
abstract::A cell-free protein-synthesizing system active in initiation of translation of both endogenous mRNA and exogenous mRNA has been obtained from postmitochondrial supernatant (S-12) of the liver of ethionine-treated rats by adding reticulocyte ribosomal extract as a source of initiation factor. Formation of polysomes in ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133837
更新日期:1982-05-01 00:00:00
abstract::We examined the inhibitory effect of three pseudo-aminosugars (validamine, valienamine, and valiolamine), which were isolated from the broth of Streptomyces hygroscopicus, on the oligosaccharide-processing glucosidases I and II involved in glycoprotein biosynthesis in rat liver. Both glucosidases I and II were inhibit...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123160
更新日期:1990-07-01 00:00:00
abstract::Transmissible spongiform encephalopathies (TSEs), or prion diseases, are fatal infectious neurodegenerative disorders. Their causative agents are prions, which are composed of disease-associated forms of prion protein (PrP(Sc)). Naturally occurring cases of TSEs are found in several mammalian species including humans,...
journal_title:Journal of biochemistry
pub_type: 杂志文章,评审
doi:10.1093/jb/mvs148
更新日期:2013-02-01 00:00:00
abstract::Mitochondria exhibiting cyanide-resistant respiration were isolated from Hansenula anomala which had been incubated in the presence of antimycin A to induce cyanide-resistant respiration. The cyanide-resistant respiration in isolated mitochondria was not inhibited by antimycin A or myxothiazol, suggesting that the bra...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123176
更新日期:1990-08-01 00:00:00
abstract::Resonance Raman spectra of the soluble-domain of a membrane-bound hydrogenase from Desulfovibrio vulgaris Miyazaki F were recorded in different oxidation states. In the oxidized state, the Raman band due to the totally symmetric stretching mode of the iron-sulfur cluster was observed at 341 cm-1, which was attributed ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123269
更新日期:1990-11-01 00:00:00
abstract::To examine whether serine proteases of rat liver chromatin are also involved in the degradation of newly synthesized and unbound ribosomal proteins and histones, like the nuclear thiol protease which we reported previously (Tsurugi, K. & Ogata, K. (1979) Eur. J. Biochem. 101, 205-213), in vivo experiments were carried...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134257
更新日期:1983-05-01 00:00:00
abstract::The contribution of the P1' residue at the first reactive site of peanut protease inhibitor B-III to the inhibition was analyzed by replacement of the P1' Arg(11) with other amino acids (Arg, Ser, Ala, Leu, Phe, Asp) after selective modification of the second reactive site. The Arg derivative had the same trypsin inhi...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/101.3.723
更新日期:1987-03-01 00:00:00
abstract::Histidine and its derivatives increased rabbit muscle fructose 1,6-bisphosphatase activity at neutral pH with positive cooperativity. In the presence of histidine and carnosine the optimum pH shifted from pH 8.0 to 7.4. The cooperative response of the enzyme to AMP and fructose 1,6-bisphosphate was observed in the pre...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a132723
更新日期:1980-01-01 00:00:00
abstract::The amino acid compositions, constituent polypeptides and bacteriochlorophyll (Bchl) contents of two kinds of Bchl-protein complexes isolated from Rhodopseudomonas palustris were examined. Spectrally dissimilar intracytoplasmic membranes obtained from cells cultured under different conditions were used as starting mat...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134110
更新日期:1982-12-01 00:00:00
abstract::A cysteine proteinase inhibitor (designated as cystatin SA) was isolated from human whole saliva by procedures including chromatography on DE 32 and DEAE-Sepharose CL-6B. The amino acid sequence determined by conventional methods showed sequence homology of 90 and 87% as compared with the sequences of cystatin S and c...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122107
更新日期:1987-10-01 00:00:00
abstract::As a first step to catalogue mRNAs present in mouse embryonal carcinoma F9 cells, 879 clones corresponding to low-abundance mRNAs were selected from among 2,896 randomly picked up clones of undifferentiated F9 cDNA libraries, using DNA probes complementary to poly(A)+RNAs prepared from undifferentiated F9 cells and to...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124485
更新日期:1994-07-01 00:00:00
abstract::Tetrahymena F-actin-binding protein, which induces bundling of Tetrahymena F-actin, was localized to a division furrow during cytokinesis. We report here the cloning and characterization of the gene and cDNA of a Tetrahymena F-actin-binding protein. The cDNA encodes a protein comprising 579 deduced amino acids with a ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022587
更新日期:2000-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134130
更新日期:1982-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123224
更新日期:1990-09-01 00:00:00
abstract::1. The relation between intrinsic viscosity in 0.2 M NaCl and molecular weight for samples of chondroitin sulfate isolated from shark cartilage could be expressed by [eeta] = 6.5 X 10(-6) Mr114. 2. Their sedimentation coefficients were linear with respect to the square root of molecular weight, and the intercept was n...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131956
更新日期:1978-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1984-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131866
更新日期:1977-12-01 00:00:00
abstract::Here we report the enzymatic and ligand-binding properties of a major secretory protein in the choroid plexus of cane toad, Bufo marinus, whose protein is homologous with lipocalin-type prostaglandin (PG) D synthase (L-PGDS) and is recombinantly expressed in Xenopus A6 cells and Escherichia coli. The toad protein boun...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvm016
更新日期:2007-02-01 00:00:00
abstract::The FLI-1 transcription factor is a member of the ETS gene family, most closely related to ERG. In this study, the FLI-1 protein products were characterized using a specific monoclonal antibody previously developed against bacterially expressed protein. In the human T-cell line Jurkat, both isoforms of FLI-1, p51 and ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvi032
更新日期:2005-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2020-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123199
更新日期:1990-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1978-06-01 00:00:00
abstract::Erucic acid was incorporated into cardiac phosphatidylserine and hepatic and cardiac sphingomyelin of male Sprague-Dawley rat. The fatty acid compositions of mitochondrial and microsomal phospholipids were similar in both liver and heart. The effect of a low fat diet and a diet containing erucic acid on the fatty acid...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133921
更新日期:1982-07-01 00:00:00
abstract::Class B scavenger receptor type I (SR-BI) is a phosphatidylserine (PS)-recognizing receptor of testicular Sertoli cells responsible for the phagocytosis of spermatogenic cells undergoing apoptosis. Here, we determined signal mediators that compose a signalling pathway for SR-BI-induced phagocytosis. Results of a yeast...
journal_title:Journal of biochemistry
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doi:10.1093/jb/mvn176
更新日期:2009-03-01 00:00:00
abstract::The internal ribosome entry site (IRES) is important for translation of hepatitis C virus (HCV) mRNA and has a unique RNA structure containing conserved domains I to IV. To investigate the function of domain II, we selected RNA aptamers that bind to domain II of HCV IRES by applying a simple and convenient selection m...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00