Abstract:
:The structure of vibrioferrin, a siderophore from Vibrio parahaemolyticus, was elucidated based on a combination of partial hydrolysis and spectroscopic techniques. HPLC of purified vibrioferrin showed two peaks with an area ratio of approximately 2:1. However, upon reinjection of each of those isolated compounds, the original chromatographic pattern was obtained, indicating an equilibrium between two compounds in aqueous solution. Consistent with this finding, most of the NMR signals of vibrioferrin were duplicated. The structure was determined as 1-(2-[2-(5-carboxy-5-hydroxy-2-oxo-1-pyrrolidinyl)propionamide]ethyl) citrate, which exists in two epimeric forms resulting from cyclization between an amidic nitrogen of the alanine residue and a keto group of the 2-ketoglutaric acid residue. Transport experiments with 55Fe-labeled vibrioferrin demonstrated the function of vibrioferrin as a siderophore in V. parahaemolyticus. Kinetic studies with mid-log phase cells revealed that the iron uptake system was receptor-mediated, with Km and Vmax values of 67 nM and 54 pmol Fe/mg cell protein/min, respectively. Moreover, iron uptake mediated by vibrioferrin was blocked both by uncouplers and by ATPase inhibitors.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Yamamoto S,Okujo N,Yoshida T,Matsuura S,Shinoda Sdoi
10.1093/oxfordjournals.jbchem.a124432subject
Has Abstractpub_date
1994-05-01 00:00:00pages
868-74issue
5eissn
0021-924Xissn
1756-2651journal_volume
115pub_type
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journal_title:Journal of biochemistry
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更新日期:1996-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135230
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doi:10.1093/oxfordjournals.jbchem.a122718
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a124958
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a021274
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1997-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a002845
更新日期:2001-02-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135523
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更新日期:2008-11-01 00:00:00
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更新日期:1999-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a130792
更新日期:1975-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1981-11-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123463
更新日期:1991-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1977-03-01 00:00:00
abstract::A beta-galactosidase was extracted from the internal organs of a sea squirt, Styela plicata, and purified 959-fold, with an 18% yield, by successive gel chromatography, anion-exchange chromatography, chromatofocusing, and affinity chromatography on a Con A-Sepharose column. The purified enzyme was fairly homogeneous, ...
journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1991-07-01 00:00:00