Increased expression of carnitine palmitoyltransferase I gene is repressed by administering L-carnitine in the hearts of carnitine-deficient juvenile visceral steatosis mice.

abstract：:I-protein was mixed with myosin before or after myosin filaments were reconstituted. In both cases, I-protein seemed to accelerate the myosin assembly. The binding of I-protein to myosin filaments was tested by sedimentation experiments and SDS-polyacrylamide gel electrophoresis. In a low ionic strength solution at pH...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1989-07-01 00:00:00

abstract：:The X-ray diffraction pattern from chromatophore membranes of a photosynthetic bacterium, Rhodospirillum rubrum, indicates that a highly organized protein assembly exists in the membrane. The X-ray scatterer was solubilized from chromatophores by a mixture of cholate and deoxycholate. The basic component was identifie...

journal_title：Journal of biochemistry

authors： Kataoka M,Ueki T

更新日期：1981-01-01 00:00:00

abstract：:Silent information regulator type-1 (SIRT1) is crucial during the development of acute respiratory distress syndrome (ARDS). We aimed to explore whether SIRT1 activation could protect against ARDS. SIRT1 was activated by its agonist SRT1720. ARDS was induced by intraperitoneal injection of 5 mg/kg lipopolysaccharide (...

journal_title：Journal of biochemistry

authors： Zhan Y,Yang C,Zhang Q,Yao L

更新日期：2021-01-22 00:00:00

abstract：:Dupuytren's disease is a palmar fibromatosis associated with changes in fibroblast activity that also affect the metabolism of extracellular matrix components. In contrast to disease connected alterations in collagen and non-collagenous glycoproteins (mainly fibronectin), the metabolism of proteoglycans, being glycosa...

journal_title：Journal of biochemistry

authors： Koźma EM,Olczyk K,Wisowski G,Głowacki A,Bobiński R

更新日期：2005-04-01 00:00:00

abstract：:Tyr225 in the active site of Escherichia coli aspartate aminotransferase (AspAT) was replaced by phenylalanine or arginine by site-directed mutagenesis. X-ray crystallographic analysis of Y225F AspAT showed that the benzene ring of Phe225 was situated at the same position as the phenol ring of Tyr225 in wild-type AspA...

journal_title：Journal of biochemistry

authors： Inoue K,Kuramitsu S,Okamoto A,Hirotsu K,Higuchi T,Morino Y,Kagamiyama H

更新日期：1991-04-01 00:00:00

abstract：:ENO1-'lacZ fusions with various lengths of the ENO1 5'-flanking region were constructed on various types of yeast plasmid vectors. The fully expressed level of beta Gal directed by ENO1-'lacZ fusions differed depending on the type of vector, but on any type of vector, beta Gal activity was not greatly influenced by th...

journal_title：Journal of biochemistry

authors： Uemura H,Shiba T,Machida M,Matsui I,Jigami Y,Tanaka H

更新日期：1987-07-01 00:00:00

abstract：:When compared with collagens isolated from animal sources, collagens and collagen-like (CL) proteins from non-animal sources are non-immunogenic and thus promising as biomedical materials. Recently, a CL protein, V-CL, was identified from a bacterial source, Streptococcus pyogenes. In this study, an acid-precipitation...

journal_title：Journal of biochemistry

authors： Ma L,Chai Y,Wu T,Wang M

更新日期：2017-09-01 00:00:00

abstract：:An inorganic pyrophosphatase [EC 3.6.1.1] was isolated from Thiobacillus thiooxidans and purified 975-fold to a state of apparent homogeneity. The enzyme catalyzed the hydrolysis of inorganic pyrophosphate and no activity was found with a variety of other phosphate esters. The cation Mg2+ was required for maximum acti...

journal_title：Journal of biochemistry

authors： Tominaga N,Mori T

更新日期：1977-02-01 00:00:00

abstract：:Lambda dv plasmids having an amber mutation in an initiator gene, O or P, were constructed from mutant lambda phages by recombinant DNA techniques and several properties of such derivatives were investigated. These plasmids are perpetuated in suppressor-plus (amber-permissive) cells, but not in non-suppressor cells. T...

journal_title：Journal of biochemistry

authors： Miwa T,Akaboshi E,Matsubara K

更新日期：1983-08-01 00:00:00

abstract：:A gene encoding an Na+/H+ antiporter was cloned from chromosomal DNA of the slightly halophilic marine bacterium Vibrio parahaemolyticus. The host was an Escherichia coli mutant that lacked both of the two major Na+/H+ antiporters, NhaA and NhaB. Untransformed mutant cells were unable to grow in the presence of 0.6 M ...

journal_title：Journal of biochemistry

authors： Kuroda T,Shimamoto T,Inaba K,Tsuda M,Tsuchiya T

更新日期：1994-11-01 00:00:00

abstract：:Plasmapheresis with a dextran sulfate column is a treatment for patients with hypercholesteremia. When proteins bound to the column during the treatment were fractionated to prepare some known proteins, we found a 57 kDa glycoprotein designated GP57 which showed a new N-terminal amino acid sequence. Western-blot analy...

journal_title：Journal of biochemistry

authors： Choi-Miura NH,Sano Y,Oda E,Nakano Y,Tobe T,Yanagishita T,Taniyama M,Katagiri T,Tomita M

更新日期：1995-02-01 00:00:00

abstract：:A spectrofluorometric method for sensitive determination of metalloendopeptidase activity has been developed by using a bimane-peptide containing a tryptophan residue, i.e. 1,7-dioxo-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-alpha]pyrazol-3-yl-methyl- thiomethylcarbonyl-phenylalanyl-tryptophanyl-leucine (Bim-SCH2CO-Phe-Trp-L...

journal_title：Journal of biochemistry

authors： Kajiwara K,Kumazaki T,Sato E,Kanaoka Y,Ishii S

更新日期：1991-09-01 00:00:00

abstract：:Synthesis of the acid-soluble proteins in the early cleavage stage of the sea urichin, Hemicentrotus pulcherrimus, was investigated. As detected by the incorporation of lysine, the acid-soluble proteins were synthesized periodically even before the first cleavage, differing from the pattern of incorporation of tryptop...

journal_title：Journal of biochemistry

authors： Kano K,Mano Y

更新日期：1976-09-01 00:00:00

abstract：:Acetoxy Drug: Protein Transacetylase (TAase) mediating the transfer of acetyl group(s) from polyphenolic acetates (PA) to certain functional proteins in mammalian cells was identified by our earlier investigations. TAase activity was characterized in the cell lysates of Mycobacterium smegmatis and the purified protein...

journal_title：Journal of biochemistry

authors： Gupta G,Baghel AS,Bansal S,Tyagi TK,Kumari R,Saini NK,Ponnan P,Kumar A,Bose M,Saluja D,Patkar SA,Parmar VS,Raj HG

更新日期：2008-12-01 00:00:00

abstract：:Two cultured cell lines, called Kan-R1 and Kan-R2, were established from rat hepatic cells by in vitro culture with a hepatocarcinogen, 3-methoxy-4-aminoazobenzene, and examined for the gene expression of cytochrome p450 (p450) isoforms, CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP3A1 and CYP3A2, by the RT-PCR method. It was r...

journal_title：Journal of biochemistry

authors： Degawa M,Namiki M,Yoshimoto N,Makino M,Iwamoto M,Nemoto K,Hashimoto Y

更新日期：2003-06-01 00:00:00

abstract：:The SoxR protein of Escherichia coli responds to redox signals by activating the transcription of soxS, which encodes another transcription activator that directly stimulates oxidative stress genes. In contrast, Pseudomonas aeruginosa has an open reading frame (ORF) encoding a putative protein homologous to E. coli So...

journal_title：Journal of biochemistry

authors： Kobayashi K,Tagawa S

更新日期：2004-11-01 00:00:00

abstract：:A high mobility group (HMG) nonhistone protein fraction HMG(1 + 2), composed of HMG1 and HMG2, was prepared from pig thymus chromatin. In order to examine a possibility that the HMG(1 + 2) participates in the unwinding of the DNA double-helix, DNA hydrolysis assay systems with the endonucleases specific for single-str...

journal_title：Journal of biochemistry

authors： Yoshida M,Shimura K

更新日期：1984-01-01 00:00:00

abstract：:The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

journal_title：Journal of biochemistry

authors： Fukumori Y,Yamanaka T

更新日期：1987-02-01 00:00:00

abstract：:The aromatic region of the NMR spectrum of bovine pancreatic ribonuclease A was analyzed in order to clarify the nature of the microenvironments surrounding the individual histidine, tyrosine, and phenylalanine residues and the interactions with inhibitors. The NMR titration curves of ring protons of six tyrosine and ...

journal_title：Journal of biochemistry

authors： Tanokura M

更新日期：1983-07-01 00:00:00

abstract：:Specific antibodies were prepared against cytochromes P450 PB-1, PB-2, PB-4, and PB-5 purified from hepatic microsomes of male rats treated with phenobarbital. With these antibodies, the levels of these four cytochrome P450s in hepatic, renal, and pulmonary microsomes of male rats that were untreated, treated with phe...

journal_title：Journal of biochemistry

authors： Imaoka S,Terano Y,Funae Y

更新日期：1989-06-01 00:00:00

abstract：:Interleukin-1 (IL-1) is produced and released by various cells, including activated macrophages, and plays important roles in inflammation as well as immune responses. Since the precursor of IL-1 has no signal peptide, the mechanism of IL-1 release has been an enigma. To investigate the possibility of direct interacti...

journal_title：Journal of biochemistry

authors： Oku N,Saito N,Okada S,Watanabe N,Kobayashi Y

更新日期：1995-10-01 00:00:00

abstract：:The aim of this work was to investigate the putative modulation of glucose uptake in trophoblast cells by several dietary compounds and by drugs of abuse. For this, the acute (26 min) and chronic (48 h) effect of these substances on the apical uptake of 3H-2-deoxy-D-glucose (3H-DG) by a human choriocarcinoma cell line...

journal_title：Journal of biochemistry

authors： Araújo JR,Gonçalves P,Martel F

更新日期：2008-08-01 00:00:00

abstract：:An acidic protein of human saliva, which we named SAP-1 previously, is now shown to be an inhibitor of several cysteine proteinases. The protein inhibited papain and ficin strongly, and stem bromelain and bovine cathepsin C partially. However, it did not inhibit either porcine cathepsin B or clostripain. The mode of t...

journal_title：Journal of biochemistry

authors： Isemura S,Saitoh E,Ito S,Isemura M,Sanada K

更新日期：1984-10-01 00:00:00

abstract：:Steady-state kinetic analyses suggest that Pseudomonas ochraceae 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (4-carboxy-2-hydroxy-cis,cis-muconate-6-semialdehyde: NADP+ oxidoreductase [EC 1.2.1.45]) functions through an ordered BiBi mechanism. The enzyme binds one NADP+ molecule per subunit with a Kd of 4...

journal_title：Journal of biochemistry

authors： Maruyama K

更新日期：1988-04-01 00:00:00

abstract：:The patterns of myosin isozymes in embryonic and adult chicken pectoralis muscle were examined by electrophoresis in a non-denaturing gel system (pyrophosphate acrylamide gel electrophoresis), and both light chains and heavy chains of embryonic and adult myosin isozymes were compared. In pyrophosphate acrylamide gel e...

journal_title：Journal of biochemistry

authors： Takano-Ohmuro H,Obinata T,Masaki T,Mikawa T

更新日期：1982-04-01 00:00:00

abstract：:Elafin was shown to be a new type of proteinase inhibitor which has an anchoring sequence. Human elafin, a potent inhibitor specific for elastase and proteinase 3, has a unique repeating sequence in its prosegment that is rich in Gln and Lys residues. The prosegment, termed "cementoin," exhibits high homology with the...

journal_title：Journal of biochemistry

authors： Nara K,Ito S,Ito T,Suzuki Y,Ghoneim MA,Tachibana S,Hirose S

更新日期：1994-03-01 00:00:00

abstract：:Using the mRNA differential display technique and Western blot analysis, the present study demonstrates that induction of KAR2 occurs when misfolded membrane-bound cytochrome P450, mutated in its cytosolically exposed domain, is expressed in Saccharomyces cerevisiae. Using various KAR2 promoter constructs in front of ...

journal_title：Journal of biochemistry

authors： Zimmer T,Ogura A,Ohta A,Takagi M

更新日期：1999-12-01 00:00:00

abstract：:The complete amino acid sequences of the Ulex europeus anti-H(O) lectins I and II were determined by using a protein sequencer. After digestion with endoproteinases Lys-C and Asp-N of the lectins reduced with 2-mercaptoethanol and modified with iodoacetamide, the resulting peptides were purified by reversed-phase high...

journal_title：Journal of biochemistry

authors： Konami Y,Yamamoto K,Osawa T

更新日期：1991-04-01 00:00:00

abstract：:Activation of antiviral innate immunity is triggered by cellular pattern recognition receptors. Retinoic acid inducible gene-I (RIG-I)-like receptors (RLRs) detect viral non-self RNA in cytoplasm of virus-infected cells and play a critical role in the clearance of the invaded viruses through production of antiviral cy...

journal_title：Journal of biochemistry

authors： Yoneyama M,Jogi M,Onomoto K

更新日期：2016-03-01 00:00:00

abstract：:To investigate the role of carboxyl groups of glucoamylases [EC 3.2.1.3] from a Rhizopus sp. (Gluc1 and Gluc2), the modification of Gluc1 and Gluc2 with a water-soluble carbodiimide, 1-cyclohexyl-3-(2-morpholinyl-(4)-ethyl)carbodiimide metho-p-toluenesulfonate (CMC), was studied. The inactivation of Gluc1 proceeded wi...

journal_title：Journal of biochemistry

authors： Iwama M,Ohtsuki R,Takahashi T,Irie M

更新日期：1984-08-01 00:00:00