Regulation of antiviral innate immune signaling by stress-induced RNA granules.

abstract：:Serum at 5 to 10% is required for maintenance of functional adult rat hepatocytes in primary culture. One effect of the serum is to induce attachment and spreading of hepatocytes on plates as monolayers. Another role is to maintain cell viability for over 2 days. For the first effect, serum could be replaced completel...

journal_title：Journal of biochemistry

authors： Horiuti Y,Nakamura T,Ichihara A

更新日期：1982-12-01 00:00:00

abstract：:A stroma-dependent B lymphoid cell line (B31-1) has been established by coculturing sorted stem cells on a novel bone marrow stromal cell line (TBR31-1). B31-1 cells express B220, but do not express other B lymphoid differentiation markers including CD43, heat stable antigen (HSA), or surface immunoglobulin (Ig) M (sI...

journal_title：Journal of biochemistry

authors： Matsuda K,Koguma M,Okuyama R,Nakazawa T,Matsuzaki Y,Nakauchi H,Yanai N,Terasaki T,Obinata M

更新日期：1999-03-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:We have detected in rat liver cytosol three enzymes (termed C-1, C-2, and C-3) which cleaved the RNA moiety of RNA-DNA hybrid. These enzymes were separated from each other by DEAE-Sephadex and Sephadex G-200 chromatography. C-1 and C-2 specifically act on the RNA moiety of RNA-DNA hybrid, while C-3 degrades single-str...

journal_title：Journal of biochemistry

authors： Tashiro F,Ueno Y

更新日期：1978-08-01 00:00:00

abstract：:2-Amino-4-methylphenol was converted to a brownish yellow material by the lysates of human erythrocytes or purified human hemoglobin. The reaction proceeded oxidatively, coupled with the oxidation of hemoglobin. The major component of the brownish yellow material produced by oxidative condensation of 2-amino-4-methylp...

journal_title：Journal of biochemistry

authors： Tomoda A,Arisawa M,Koshimura S

更新日期：1991-12-01 00:00:00

abstract：:1. When ribonuclease T1 [EC 3.1.4.8] (0.125% solution) was treated with a 760-fold molar excess of iodoacetamide at pH 8.0 and 37 degrees, about 90% of the original activity was lost in 24 hr. The half-life of the activity was about 8 hr. The binding ability for 3'-GMP was lost simultaneously. Changes were detected on...

journal_title：Journal of biochemistry

authors： Takahashi K

更新日期：1976-12-01 00:00:00

abstract：:Kinetic analyses of the hydrolysis reactions of N-acetylated and N-deacetylated derivatives of 4-methylumbelliferyl chitobioside [(GlcNAc)(2)-UMB (1), GlcN-GlcNAc-UMB (2), GlcNAc-GlcN-UMB (3), and (GlcN)(2)-UMB (4)] by ChiA and ChiB from Serratia marcescens were performed. Both enzymes released UMB from all compounds ...

journal_title：Journal of biochemistry

authors： Honda Y,Kitaoka M,Tokuyasu K,Sasaki C,Fukamizo T,Hayashi K

更新日期：2003-02-01 00:00:00

abstract：:Four glycolipids (Fr. I, II, III, and IV) were isolated from whale brain and three (Fr. I, II, and III) of them were demonstrated to be galactosyl ceramide combined with fatty acid by ester linkage. Fr. IV was shown to be a monoalkyl monoacyl galactosyl glycerol. For determining the substituted position of the acyl gr...

journal_title：Journal of biochemistry

authors： Yasugi E,Saito E,Kasama T,Kojima H,Yamakawa T

更新日期：1982-04-01 00:00:00

abstract：:The internal structure of the recombinant yeast-derived human hepatitis B virus surface antigen vaccine particle was investigated by small-angle neutron scattering using the contrast variation method. Data were collected in aqueous buffer solutions containing 0, 40, 60, and 100% D2O in the q range of 0.005 to 0.2 A-1 ...

journal_title：Journal of biochemistry

authors： Sato M,Sato-Miyamoto Y,Kameyama K,Ishikawa N,Imai M,Ito Y,Takagi T

更新日期：1995-12-01 00:00:00

abstract：:Homogeneous indanol dehydrogenase from monkey liver catalyzed the reversible conversion of 3 alpha- or 20 alpha-hydroxy groups of several bile acids and 5 beta-pregnanes to the corresponding 3- or 20-ketosteroids. The kcat values for the steroids determined at pH 7.4 were low, but the kcat/Km values for the 3-ketoster...

journal_title：Journal of biochemistry

authors： Hara A,Nakagawa M,Taniguchi H,Sawada H

更新日期：1989-11-01 00:00:00

abstract：:A major lipid class in the Harderian glands of the Mongolian gerbil was investigated. The IR and 1H-NMR spectra suggested that it was a wax-like compound. Fatty acids present were capric, lauric, myristic, palmitic, stearic, arachidic, and behenic acids in ratios of 3.0%, 16.3%, 16.5%, 29.5%, 5.1%, 16.4%, and 9.8%, re...

journal_title：Journal of biochemistry

authors： Otsuru O,Otsuka H,Kasama T,Seyama Y,Sakai T,Yohro T

更新日期：1983-12-01 00:00:00

abstract：:Antizyme (AZ) interacts with ornithine decarboxylase, which catalyzes the first step of polyamine biosynthesis and recruits it to the proteasome for degradation. Synthesizing the functional AZ protein requires transition of the reading frame at the termination codon. This programmed +1 ribosomal frameshifting is induc...

journal_title：Journal of biochemistry

authors： Oguro A,Shigeta T,Machida K,Suzuki T,Iwamoto T,Matsufuji S,Imataka H

更新日期：2020-08-01 00:00:00

abstract：:The accumulation of unfolded proteins in the endoplasmic reticulum (ER) under ER stress conditions activates a series of homoeostatic responses collectively termed the unfolded protein response (UPR). The UPR is unique in which the molecular mechanisms it uses to transmit signals from the ER lumen to the nucleus are c...

journal_title：Journal of biochemistry

authors： Mori K

更新日期：2009-12-01 00:00:00

abstract：:Acetoxy Drug: Protein Transacetylase (TAase) mediating the transfer of acetyl group(s) from polyphenolic acetates (PA) to certain functional proteins in mammalian cells was identified by our earlier investigations. TAase activity was characterized in the cell lysates of Mycobacterium smegmatis and the purified protein...

journal_title：Journal of biochemistry

authors： Gupta G,Baghel AS,Bansal S,Tyagi TK,Kumari R,Saini NK,Ponnan P,Kumar A,Bose M,Saluja D,Patkar SA,Parmar VS,Raj HG

更新日期：2008-12-01 00:00:00

abstract：:Thymidine kinase [ATP: thymidine 5'-phosphotransferase, EC 2.7.1.21] has been purified more than 3,500 fold from microplasmodia of Physarum polycephalum. Properties of the enzyme were determined on preparations purified 1,400 fold. Thymidine was transformed to dTMP while a stoichiometric quantity of ATP was transforme...

journal_title：Journal of biochemistry

authors： Chraibi R,Wright M

更新日期：1983-02-01 00:00:00

abstract：:We previously reported that sera from various kinds of animals contain a protein(s) capable of inhibiting the growth of the non-malignant epithelial cell line derived from Buffalo rat liver (BRL). In the present study, a similar epithelial cell-specific growth inhibitor (EGI) was purified to homogeneity from an acid-e...

journal_title：Journal of biochemistry

authors： Huang W,Kimura T,Mashima K,Miyazaki K,Masaki H,Yamashita J,Horio T

更新日期：1986-09-01 00:00:00

abstract：:Thymosin β4 (Tβ4) is a major intracellular G-actin-sequestering peptide. There is increasing evidence to support important extracellular functions of Tβ4 related to angiogenesis, wound healing and cardiovascular regeneration. We investigated the expression of 'Tβ4' and 'thymosin β10', a closely related peptide, during...

journal_title：Journal of biochemistry

authors： Tokura Y,Nakayama Y,Fukada S,Nara N,Yamamoto H,Matsuda R,Hara T

更新日期：2011-01-01 00:00:00

abstract：:Previous studies have shown that purified mitochondrial outer membrane is able to catalyze the transfer of sialic acid from CMP-Neu5Ac to an exogenous asialoglycoprotein acceptor, asialofetuin. Considering the heterogeneity of the glycan chains borne by this glycoprotein, an investigation of mitochondrial sialyltransf...

journal_title：Journal of biochemistry

authors： Gasnier F,Baubichon-Cortay H,Louisot P,Gateau-Roesch O

更新日期：1991-11-01 00:00:00

abstract：:We have studied the regio- and stereoselectivity of ring-hydroxylation and N-desisopropylation of S(-)- and R(+)-propranolol, using dog liver microsomes and the expressed dog CYP2D15 in insect cells. In dog liver microsomes, 4-hydroxylation was the preferred pathway in S(-)-propranolol oxidation, while N-desisopropyla...

journal_title：Journal of biochemistry

authors： Tasaki T,Iwata H,Kazusaka A,Fujita S

更新日期：1998-04-01 00:00:00

abstract：:Protein S, a vitamin K-dependent plasma protein having Gla-residues, increases the rate of inactivation of Factor Va by activated protein C by enhancing the binding of activated protein C to phospholipid [Walker, J.F. (1981) J. Biol. Chem. 256, 11128-11131]. The present study aimed at elucidating the effect of thrombi...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Hashimoto S

更新日期：1983-09-01 00:00:00

abstract：:The amino acid compositions, constituent polypeptides and bacteriochlorophyll (Bchl) contents of two kinds of Bchl-protein complexes isolated from Rhodopseudomonas palustris were examined. Spectrally dissimilar intracytoplasmic membranes obtained from cells cultured under different conditions were used as starting mat...

journal_title：Journal of biochemistry

authors： Hayashi H,Nakano M,Morita S

更新日期：1982-12-01 00:00:00

abstract：:Terf/TRIM17 is a tripartite motif protein that has been originally isolated from testis. Terf has been characterized to exhibit an E3 ubiquitin ligase activity and to undergo self-ubiquitination. The cellular function of terf and its substrates, however, remain elusive. In the present study, we performed a yeast two-h...

journal_title：Journal of biochemistry

authors： Endo H,Ikeda K,Urano T,Horie-Inoue K,Inoue S

更新日期：2012-02-01 00:00:00

abstract：:A partially purified fraction (Component I) which catalyzed the ATP-PPi exchange reaction dependent only on valine and glycine among the five constituent amino acids of gramicidin A was obtained from crude extracts of Bacillus brevis ATCC 8185, which produces both gramicidin A and tyrocidines. This fraction was found ...

journal_title：Journal of biochemistry

authors： Akashi K,Kubota K,Kurahashi K

更新日期：1977-01-01 00:00:00

abstract：:We have developed a screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli. In this system, we used a plasmid library to express artificial sRNAs (approximately 200 bases long) containing 60 bases of random nucleotide sequence. The induced expression of the known rydB sRNA in th...

journal_title：Journal of biochemistry

authors： Komasa M,Fujishima K,Hiraoka K,Shinhara A,Lee BS,Tomita M,Kanai A

更新日期：2011-09-01 00:00:00

abstract：:The genes coding for the phospholipid degradation enzymes in E. coli, detergent-resistant (DR-) phospholipase A (pldA) and lysophospholipase L2 (pldB), were cloned together on the plasmid pKO1 (Homma, H., Kobayashi, T., Ito, Y., Kudo, I., Inoue, K., Ikeda, H., Sekiguchi, M., & Nojima, S. (1983) J. Biochem. 94, 2079-20...

journal_title：Journal of biochemistry

authors： Kobayashi T,Kudo I,Homma H,Karasawa K,Inoue K,Ikeda H,Nojima S

更新日期：1985-10-01 00:00:00

abstract：:1. From the standpoint of monomer-dimer equilibrium of hog kidney D-amino acid oxidase [EC 1.4.3.3] and the interaction between the enzyme and small molecules, the effect of pH on the binding of p-aminobenzoate to the monomer and dimer of the enzyme was studied by kinetic methods and spectrophotometric titration. 2. T...

journal_title：Journal of biochemistry

authors： Horiike K,Shiga K,Isomoto A,Yamano T

更新日期：1976-11-01 00:00:00

abstract：:We have unexpectedly found that Streptomyces subtilisin inhibitor (SSI) and some other similar serine protease inhibitors produced by Streptomycetes strongly inhibit Streptomyces griseus metallo-endopeptidase II (SGMP II) [Kajiwara, K. et al. (1991) J. Biochem. 110, 350-354]. In order to elucidate the mode of their un...

journal_title：Journal of biochemistry

authors： Kumazaki T,Kajiwara K,Kojima S,Miura K,Ishii S

更新日期：1993-10-01 00:00:00

abstract：:The internal ribosome entry site (IRES) is important for translation of hepatitis C virus (HCV) mRNA and has a unique RNA structure containing conserved domains I to IV. To investigate the function of domain II, we selected RNA aptamers that bind to domain II of HCV IRES by applying a simple and convenient selection m...

journal_title：Journal of biochemistry

authors： Kikuchi K,Umehara T,Fukuda K,Hwang J,Kuno A,Hasegawa T,Nishikawa S

更新日期：2003-03-01 00:00:00

abstract：:Catalase from Aspergillus niger was purified to homogeneity as judged from the results of ultracentrifugation and polyacrylamide gel electrophoresis. The enzyme had a molecular weight of 385,000 as estimated from sedimentation measurements. Carbohydrate analyses showed that the catalase was a glycoprotein containing a...

journal_title：Journal of biochemistry

authors： Kikuchi-Torii K,Hayashi S,Nakamoto H,Nakamura S

更新日期：1982-11-01 00:00:00

abstract：:The effects of D-glucose anomers on the germination of dormant spores of Bacillus megaterium QM B1551 were studied, alpha-D-Glucose (1 mM) slightly initiated the germination of the dormant spores during 10 min incubation at 37 degrees C, while about 60% of the dormant spores became germinated with beta-D-glucose (1 mM...

journal_title：Journal of biochemistry

authors： Miwa I,Ishihara H,Fujii H,Nagano H,Okuda J

更新日期：1979-01-01 00:00:00