Interaction between D-amino acid oxidase and small molecules.

abstract：:Human promyelocytic leukemia cells (HL-60) were treated with several differentiation inducers, then the changes in the activity of cytosolic protein kinase C (PKC) isoforms were examined by hydroxylapatite chromatography and the species of the isoforms were determined immunologically. In three undifferentiated HL-60 c...

journal_title：Journal of biochemistry

authors： Tanaka Y,Yoshihara K,Tsuyuki M,Itaya-Hironaka A,Inada Y,Kamiya T

更新日期：1992-02-01 00:00:00

abstract：:Basigin belongs to the immunoglobulin superfamily and may be related to the primordial form of the superfamily. Human basigin cDNA was isolated and sequenced, and the predicted protein structure was compared with that of mouse basigin and two related molecules, embigin and the chicken blood-brain barrier antigen HT7. ...

journal_title：Journal of biochemistry

authors： Miyauchi T,Masuzawa Y,Muramatsu T

更新日期：1991-11-01 00:00:00

abstract：:Four motor proteins, MotX, MotY, PomA, and PomB, have been identified as constituents of the Na(+)-driven flagellum of Vibrio species. In this study, the complete motX gene was cloned from Vibrio alginolyticus and shown to complement three mot mutations, motX94, motX115, and motX119, as well as a V. parahaemolyticus m...

journal_title：Journal of biochemistry

authors： Okabe M,Yakushi T,Asai Y,Homma M

更新日期：2001-12-01 00:00:00

abstract：:D-type cyclin-dependent kinases (Cdk4 and Cdk6) regulate the G1 to S phase progression of the mammalian cell cycle. It has been suggested that Cdk4 and Cdk6 may have distinct functions in vivo, even though they are indistinguishable biochemically. Here we show that although these Cdks phosphorylate multiple residues i...

journal_title：Journal of biochemistry

authors： Takaki T,Fukasawa K,Suzuki-Takahashi I,Semba K,Kitagawa M,Taya Y,Hirai H

更新日期：2005-03-01 00:00:00

abstract：:Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ de...

journal_title：Journal of biochemistry

authors： Tanaka Y,Maruyama K,Mikawa T,Hotta Y

更新日期：1988-10-01 00:00:00

abstract：:Specific and high titer antisera against 4-O-methylglucuronic acid-containing glycosphingolipid (Lipid IV) from spermatozoa of the fresh-water bivalve, Hyriopsis schlegelii, were raised in rabbits. The antisera were found to agglutinate spermatozoa of three fresh-water bivalves, H. schlegelii, Anodonta woodiana, and C...

journal_title：Journal of biochemistry

authors： Sugita M,Inoue T,Itasaka O,Hori T

更新日期：1984-03-01 00:00:00

abstract：:Previously, we demonstrated that the 2.6-kDa peptide extending from Arg177 to Tyr198 in subdomain 4 of rabbit skeletal actin bound to actin itself, inhibited the elongation of actin filament, and severed F-actin. The corresponding segment in actin, therefore, is thought to contain the most critical actin-actin contact...

journal_title：Journal of biochemistry

authors： Hori K,Morita F,Matsuzawa F,Aikawa S

更新日期：1995-12-01 00:00:00

abstract：:Monkey (Macaca fuscata) mononuclear leukocytes were stimulated to produce thromboplastin (tissue factor) upon exposure to lipopolysaccharide, LPS. The stimulation was dose-related in the concentration range of 10(-5) to 10(-1) micrograms/ml of LPS. Lipid A portion of the LPS molecule was essential to induce the leukoc...

journal_title：Journal of biochemistry

authors： Nakamura S,Gotoh S,Takenaka O,Takahashi K

更新日期：1985-06-01 00:00:00

abstract：:The amino acid compositions, constituent polypeptides and bacteriochlorophyll (Bchl) contents of two kinds of Bchl-protein complexes isolated from Rhodopseudomonas palustris were examined. Spectrally dissimilar intracytoplasmic membranes obtained from cells cultured under different conditions were used as starting mat...

journal_title：Journal of biochemistry

authors： Hayashi H,Nakano M,Morita S

更新日期：1982-12-01 00:00:00

abstract：:Specific antibodies were prepared against cytochromes P450 PB-1, PB-2, PB-4, and PB-5 purified from hepatic microsomes of male rats treated with phenobarbital. With these antibodies, the levels of these four cytochrome P450s in hepatic, renal, and pulmonary microsomes of male rats that were untreated, treated with phe...

journal_title：Journal of biochemistry

authors： Imaoka S,Terano Y,Funae Y

更新日期：1989-06-01 00:00:00

abstract：:Kinetic analyses of the hydrolysis reactions of N-acetylated and N-deacetylated derivatives of 4-methylumbelliferyl chitobioside [(GlcNAc)(2)-UMB (1), GlcN-GlcNAc-UMB (2), GlcNAc-GlcN-UMB (3), and (GlcN)(2)-UMB (4)] by ChiA and ChiB from Serratia marcescens were performed. Both enzymes released UMB from all compounds ...

journal_title：Journal of biochemistry

authors： Honda Y,Kitaoka M,Tokuyasu K,Sasaki C,Fukamizo T,Hayashi K

更新日期：2003-02-01 00:00:00

abstract：:The melanocortin receptor type 4 (MC4-R) is involved in food intake and represents a potential target for the treatment of some forms of obesity. The fluorescent protein EGFP was fused to the wild-type or mutated coding sequence of the human MC4-R. After transfection in HEK 293, clones stably expressing hMC4-R-EGFP we...

journal_title：Journal of biochemistry

authors： Blondet A,Doghman M,Rached M,Durand P,Bégeot M,Naville D

更新日期：2004-04-01 00:00:00

abstract：:Aberrant DNA methylation is a common form of epigenetic alterations and it has been proved to be closely related to many cancers, while its role in epidermal growth factor receptor (EGFR)-mutated non-small cell lung cancer (NSCLC) is not clear. This study focuses on the role of DNA methyltransferase 1 (DNMT1) in EGFR-...

journal_title：Journal of biochemistry

authors： Wu XY,Chen HC,Li WW,Yan JD,Lv RY

更新日期：2020-08-01 00:00:00

abstract：:An O-acetylated ganglioside that generated a trisialyllactosylceramide or GT3 by base treatment was found for the first time in cod fish brain. This ganglioside was isolated by high-performance liquid chromatography, and characterized by fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance sp...

journal_title：Journal of biochemistry

authors： Waki H,Murata A,Kon K,Maruyama K,Kimura S,Ogura H,Ando S

更新日期：1993-04-01 00:00:00

abstract：:Raman spectroscopy was used to investigate the hydrogen bonding at the C(4)=O moiety of the isoalloxazine nucleus in a series of flavins and flavoproteins. Isotope effects of Raman bands confirmed that the band observed around 1,710 cm(-1) is mainly derived from C(4)=O stretching vibrational mode. A linear correlation...

journal_title：Journal of biochemistry

authors： Hazekawa I,Nishina Y,Sato K,Shichiri M,Miura R,Shiga K

更新日期：1997-06-01 00:00:00

abstract：:Glutamine synthetase from a Gram-positive acid-fast bacterium, Mycobacterium smegmatis, was purified to homogeneity from cells grown with glycerol-bouillon medium. Electron micrographs of the enzyme revealed a dodecameric arrangement of its subunits in two superimposed hexagonal rings, similar to the structure of glut...

journal_title：Journal of biochemistry

authors： Kimura K,Yagi K,Matsuoka K

更新日期：1984-06-01 00:00:00

abstract：:A post-proline cleaving enzyme [prolyl endopeptidase, EC 3.4.21.26] was purified about 3,700-fold from an extract of bovine brain by a series of column chromatographies on DEAE-Sephadex, hydroxyapatite and PCMB-T-Sepharose, and gel filtration on Sephadex G-200 using N-carbobenzoxy-Gly-Pro-beta-naphthylamide (Z-Gly-Pro...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Nishimura T,Kita T,Tsuru D

更新日期：1983-10-01 00:00:00

abstract：:Tardigrades, a phylum of meiofaunal organisms, exhibit extraordinary tolerance to various environmental conditions, including extreme temperatures (-272 to 151 °C) and exposure to ionizing radiation. Proteins from anhydrobiotic tardigrades with homology to known proteins from other organisms are new potential targets ...

journal_title：Journal of biochemistry

authors： Kobayashi K,Kim J,Fukuda Y,Kozawa T,Inoue T

更新日期：2021-01-21 00:00:00

abstract：:Aggrecan, a large aggregating proteoglycan, is one of the major structural components of cartilage. Its core protein contains three glubular domains and two glycosaminoglycan-attachment domains. These domains play various roles to maintain cartilage structure and function. An N-terminal globular domain binds hyalurona...

journal_title：Journal of biochemistry

authors： Watanabe H,Yamada Y,Kimata K

更新日期：1998-10-01 00:00:00

abstract：:Isoleucyl-tRNA formation and isoleucine-dependent PPi-ATP exchange catalyzed by purified isoleucyl-tRNA synthetase [EC 6.1.1.5] of Escherichia coli were studied in the presence of various amounts of either Mg2+, Ca2+, Fe2+, Ni2+, or Cu2+. In the presence of Mg2+, isoleucine-dependent PPi-ATP exchange was observed in p...

journal_title：Journal of biochemistry

authors： Takeda Y,Ohnishi T,Ogiso Y

更新日期：1976-09-01 00:00:00

abstract：:The effect of pyocin R1 on the glucose metabolism of sensitive Pseudomonas cells was investigated. Upon treatment with pyocin R1, although the rate of O2 uptake of the sensitive cells for glucose or gluconate was not very much affected at first, the final level of O2 uptake was greatly reduced. When 2-oxogluconate was...

journal_title：Journal of biochemistry

authors： Kageyama M

更新日期：1978-12-01 00:00:00

abstract：:We demonstrate here that ribosomes from not only Escherichia coli and Thermus thermophilus [Nitta et al. (1994) J. Biochem. 115, 803-807; ibid., (1995) 118, 841-849] but also yeast and bovine mitochondria catalyze peptide synthesis promoted by a high concentration of pyridine in the absence of soluble protein factors ...

journal_title：Journal of biochemistry

authors： Nojima T,Nitta I,Ueda T,Watanabe K

更新日期：1996-06-01 00:00:00

abstract：:We tried to confirm the antioxidative capability of lecithin:cholesterol acyltransferase (LCAT) reported by Vohl et al. [Biochemistry (1999) 38, 5976-5981]. The enzyme solution protected LDL against oxidation. However, this protection was not due to LCAT enzyme, but to some unknown low-molecular-weight substance(s) in...

journal_title：Journal of biochemistry

authors： Murakami Y,Kamiyama S,Howlader ZH,Yamato T,Komai M,Furukawa Y

更新日期：2002-01-01 00:00:00

abstract：:NADPH oxidase was induced in HL-60 human promyelocytic leukemia cells when these cells were treated with 10(-7) M 1,25-dihydroxyvitamin D3 (VD3) for 4 days. The treated cells were disrupted by sonication, and the postnuclear fraction was separated into 48,000 X g supernatant (cytosol) and precipitate (membrane) fracti...

journal_title：Journal of biochemistry

authors： Nozawa R,Kato H,Yokota T

更新日期：1988-01-01 00:00:00

abstract：:Cation specificity and effects of pH and temperature on membrane aggregation of porcine intestinal brush borders were studied in terms of the turbidity change of the membrane suspensions due to the addition of polyvalent cations. Monovalent cations (K+ and Na+) did not induce the membrane aggregation at 5 mM or below....

journal_title：Journal of biochemistry

authors： Ohyashiki T,Takeuchi M,Mohri T

更新日期：1984-03-01 00:00:00

abstract：:We studied the effect of Ca2+ on the reactivities of two SH groups of DTNB light chain (Cys 128 and Cys 157) using a fluorogenic thiol reagent. It was found that a Ca2+-induced change in reactivity occurred only with Cys 128 when the light chain was in an isolated state, whereas it occurred with both Cys 128 and Cys 1...

journal_title：Journal of biochemistry

authors： Yamamoto K,Honjo R,Sekine T

更新日期：1980-01-01 00:00:00

abstract：:The position in the acyl phosphate linkage of the phosphorylated intermediate of (Na+, K+)-ATPase that is cleaved by N-methylhydroxylamine was compared with that of the model compound acetylphosphate. The products of the cleavage of the phosphoenzyme by methylhydroxylamine were the active enzyme and a N-P compound, no...

journal_title：Journal of biochemistry

authors： Ushimaru M,Shinohara Y,Fukushima Y

更新日期：1997-09-01 00:00:00

abstract：:The mechanism by which GTP induces Ca2+ release from Ca2(+)-preloaded rat hepatic microsomes was studied. In the same concentration range as that for Ca2+ release, GTP inhibited the initial rate of ATP-driven Ca2+ uptake. It also inhibited the formation by ATP of the phosphorylated intermediate of Ca2(+)-ATPase, which...

journal_title：Journal of biochemistry

authors： Kimura S,Higham S,Robison BC,Kraus-Friedmann N

更新日期：1990-04-01 00:00:00

abstract：:Both beef heart cytochrome oxidase and bacteriorhodopsin of Halobacterium halobium were reconstituted into liposomes by the sonication-cholate dialysis method. The proteoliposomes showed the respiratory control ratio of 4.2, and steady-state illumination of the vesicles lead to the 2.7-fold stimulation of the oxidase ...

journal_title：Journal of biochemistry

authors： Miki T,Orii Y,Mukohata Y

更新日期：1987-07-01 00:00:00

abstract：:The aminopeptidase P gene in Escherichia coli HB101 was cloned into the plasmid pBR322. Introduction of the hybrid plasmid, pAPP01, into the E. coli DH1 resulted in an 8-fold increase of aminopeptidase P activity as compared with that of the host. The enzyme was purified by series of chromatographies on DEAE-Sephadex,...

journal_title：Journal of biochemistry

authors： Yoshimoto T,Murayama N,Honda T,Tone H,Tsuru D

更新日期：1988-07-01 00:00:00