A mechanism of respiratory control: studies with proteoliposomes containing cytochrome oxidase and bacteriorhodopsin.

abstract：:We previously reported the utilization of antisense RNA in the development of a novel immune system against RNA coliphage SP proliferation (Hirashima et al. [1986] Proc. Natl. Acad. Sci. U.S. 83, 7726-7730). We attempted to determine the most effective (i.e., those eliciting antiviral activity) sequences for targeting...

journal_title：Journal of biochemistry

authors： Hirashima A,Sawaki S,Mizuno T,Houba-Herin N,Inouye M

更新日期：1989-07-01 00:00:00

abstract：:Ion-exchange column-purified I-protein was labeled by fluorescein isothiocyanate (FITC) at an equimolar ratio. When FITC-labeled I-protein was reacted with glycerinated myofibrils of chicken breast muscle in a phosphate-buffered saline, fluorescence was observed at the A-band and/or the Z-line of the sarcomere. Howeve...

journal_title：Journal of biochemistry

authors： Ohashi K,Ishikawa K,Maruyama K

更新日期：1988-02-01 00:00:00

abstract：:1. Porcine cardiac native tropomyosin was phosphorylated by bovine cardiac 3':5'-cyclic AMP-dependent protein kinase. Most of the phosphate incorporation was observed in troponin I, the maximum of which was 0.7 mol of Pi per mol of troponin I. 2. In the presence of phosphorylated native tropomyosin, actomyosin ATPase ...

journal_title：Journal of biochemistry

authors： Yamamoto K,Ohtsuki I

更新日期：1982-05-01 00:00:00

abstract：:Calpastatin, an endogenous inhibitor protein specifically acting on calpain [EC 3.4.22.17; Ca2+-dependent cysteine proteinase], was purified to apparent homogeneity from the cytosol fraction of human erythrocytes. The yield was 0.38 mg from 400 ml of blood. The purification procedures included DEAE-cellulose and Ultro...

journal_title：Journal of biochemistry

authors： Takano E,Murachi T

更新日期：1982-12-01 00:00:00

abstract：:Previously, several workers reported that at very low ionic strength and in the presence of ATP, the extent of binding of S-1 with the F-actin-tropomyosin-troponin complex or regulated actin (FA-TM-TN) is unaffected by removal of Ca2+. However, in this study we found that during the ATPase reaction at physiological io...

journal_title：Journal of biochemistry

authors： Inoue A,Tonomura Y

更新日期：1982-04-01 00:00:00

abstract：:The kinetic properties of the hydrolyses of 8-Br ATP and 8-SCH3 ATP by myosin [EC 3.6.1.3] and actomyosin were compared with those of ATP, and the following results were obtained. The Ca-NTPase activities of myosin using these two ATP analogs as substrates were smaller than that of ATPase, and the NTPase activities to...

journal_title：Journal of biochemistry

authors： Takenaka H,Ikehara M,Tonomura Y

更新日期：1976-12-01 00:00:00

abstract：:A simple and rapid purification method for human serum C-reactive protein (CRP) was developed. CRP was strongly adsorbed on a DEAE-cellulose column and was easily separated from other serum proteins. CRP was purified approximately 1,000-fold with a high yield (50%). The final preparation showed a single band as judged...

journal_title：Journal of biochemistry

authors： Tsujimoto M,Inoue K,Nojima S

更新日期：1983-11-01 00:00:00

abstract：:The lanthanide ions Lu3+ (diamagnetic) and Gd3+ (paramagnetic broadening probe) were used to displace Ca2+ from the high-affinity cation binding site on G-actin. The effects of these higher-affinity ions on the proton nuclear magnetic resonance spectrum of actin were recorded. The aliphatic proton envelope in the Gd-a...

journal_title：Journal of biochemistry

authors： Barden JA,dos Remedios CG

更新日期：1984-09-01 00:00:00

abstract：:The changes in the cellular concentrations of ATP, ADP, and AMP and in oxidative phosphorylation of mitochondria were investigated in the remaining liver of partially hepatectomized rabbits. The energy charge (defined as half of the average number of anhydride-bonded phosphate groups per adenosine moiety) of the liver...

journal_title：Journal of biochemistry

authors： Kamiyama Y,Ozawa K,Honjo I

更新日期：1976-10-01 00:00:00

abstract：:Mitochondrial porin is a major integral membrane protein of the outer membrane. To assess the stop-transfer sequence in the yeast porin molecule (P), we constructed the following chimeric proteins. (i) The signal sequence of interleukin 2, a secretory protein, was fused to the amino-terminus of porin (SP). (ii) The ma...

journal_title：Journal of biochemistry

authors： Sakaguchi M,Hachiya N,Mihara K,Omura T

更新日期：1992-08-01 00:00:00

abstract：:Effects of anion transport inhibitors on hemolysis of human erythrocytes at 200 mPa were examined. The degree of hemolysis was decreased by treating intact cells with 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS), 4,4'-dinitrostilbene-2,2'-disulfonate and pyridoxal 5'-phosphate (PLP) had little effect on the hem...

journal_title：Journal of biochemistry

authors： Yamaguchi T,Matsumoto M,Kimoto E

更新日期：1995-10-01 00:00:00

abstract：:The mammalian ATP/ADP translocator isoform-2 (ANT2) gene is growth-activated. Regulation of the gene appears to involve Sp1, as an essential activator, and a suppressor through an Sp1 core element next to the transcription start. We show here that the proximal promoter also binds AP-2 strongly and specifically to two ...

journal_title：Journal of biochemistry

authors： Li R,Hodny Z,Zaid A,Barath P,Nelson BD

更新日期：1999-07-01 00:00:00

abstract：:An acid proteinase different from pepsin was purified from bullfrog (Rana catesbeiana) gastric mucosa by chromatography on hydroxyapatite, Q-Sepharose, Con A-Sepharose 4B, and Mono Q columns. Its molecular weight after purification was estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis to be 45 kDa...

journal_title：Journal of biochemistry

authors： Inokuchi T,Kobayashi K,Horiuchi S

更新日期：1994-01-01 00:00:00

abstract：:The effects of N,N'-dicyclohexylcarbodiimide (DCCD) on light-induced H+-transport and transmembrane electric potential (delta phi) formation were studied in the membrane vesicles of Halobacterium halobium R1M1. In accordance with our previous finding of the existence of two DCCD-binding components in vesicle membrane ...

journal_title：Journal of biochemistry

authors： Murakami N,Konishi T

更新日期：1985-10-01 00:00:00

abstract：:1) Two forms of acid beta-galactosidase [EC 3.1.23] with different molecular weights catalyzing the hydrolysis of GM1-ganglioside and p-nitrophenyl-beta-D-galactoside were separated and purified from porcine spleen. 2) The apparent molecular weights were 400,000-600,000 and 70,000-74,000 for the high (termed Am form) ...

journal_title：Journal of biochemistry

authors： Yamamoto Y,Fujie M,Nishimura K

更新日期：1982-07-01 00:00:00

abstract：:Endonuclease V (Endo V) is a DNA repair enzyme that recognizes deoxyinosine and cleaves the second phosphodiester bond on the 3' side of the deaminated base lesion. A database search revealed the presence of homologous genes for Endo V in most archaeal species, but the absence in some methanogenic species. We cloned a...

journal_title：Journal of biochemistry

authors： Kiyonari S,Egashira Y,Ishino S,Ishino Y

更新日期：2014-05-01 00:00:00

abstract：:N tau-Methylhistidine(3-methylhistidine) in urine of the rat is mainly derived from the degradation of actin and myosin in skeletal muscle, intestine and skin. The fractional degradation rates of the myosin-actin pools of these tissues were calculated from the time course of increase in the specific radioactivities of...

journal_title：Journal of biochemistry

authors： Nagasawa T,Funabiki R

更新日期：1981-04-01 00:00:00

abstract：:Ovomucoid-trypsin association complex was prepared by incubating chicken egg white ovomucoid with bovine trypsin. The reactivity of ovomucoid-trypsin complex was investigated by immunodiffusion, quantitative precipitation and enzyme-linked immunosorbent assay (ELISA). It was demonstrated that the association of trypsi...

journal_title：Journal of biochemistry

authors： Matsuda T,Watanabe K,Nakamura R

更新日期：1983-07-01 00:00:00

abstract：:We prepared human rheumatoid arthritic synovial fluid phospholipase A2 (PLA2) [EC 3.1.1.4] from insect cells infected with a recombinant baculovirus. The PLA2 DNA was designed, changing the original codons to those used frequently in the polyhedrin gene. Sixteen oligo-deoxynucleotides ranging from 40 to 70 nucleotides...

journal_title：Journal of biochemistry

authors： Kawauchi Y,Takasaki J,Matsuura Y,Masuho Y

更新日期：1994-07-01 00:00:00

abstract：:When an aqueous solution of a spin-labeled "two tail" gangliosidoid was incubated with liposomes or sheep erythrocytes, the broad single resonance line in the ESR spectrum disappeared and a signal showing an anisotropic motion appeared, indicating that the spin-labeled "two tail" gangliosidoid in the micellar state wa...

journal_title：Journal of biochemistry

authors： Kanda S,Inoue K,Nojima S,Utsumi H,Wiegandt H

更新日期：1982-06-01 00:00:00

abstract：:A rapid micro-assay method for gelatinolytic activitiy has been developed using 3H-labeled heat-denatured polymeric collagen (gelatin) as a substrate to investigate enzymes involved in the post-collagenase catabolism of collagen. The method is based on the incubation of gelatin with enzyme followed by determination of...

journal_title：Journal of biochemistry

authors： Sunada H,Nagai Y

更新日期：1980-06-01 00:00:00

abstract：:A fifth and new DFP-sensitive alkaline proteinase E, with strong esterase activity toward Ac-(Ala)3-OMe was found in pronase, a protease mixture from St. griseus K-1. Proteinase E was shown to be different from the elastase [EC 3.4.21.11]-like enzyme or subtilisin [EC 3.4.21.14]like enzyme, and alkaline proteinase A, ...

journal_title：Journal of biochemistry

authors： Narahashi Y,Yoda K

更新日期：1976-05-01 00:00:00

abstract：:Incubation of rat liver microsomes with GDP-[14C]mannose and phenyl phosphate for 30 min, followed by paper-chromatographic analysis, revealed the presence of a new radioactive compound that was isolated and characterized as phenyl beta-D-mannosyl phosphate. The enzyme catalyzing this reaction was very closely related...

journal_title：Journal of biochemistry

authors： Kato S,Tsuji M,Nakanishi Y,Suzuki S

更新日期：1980-03-01 00:00:00

abstract：:Striped bonito protamine, sardaine, was isolated from the sperm of striped bonito (Sarda orientalis) by extraction with sulfuric acid followed by ion-exchange chromatography. The preparation gave a single band upon polyacrylamide gel electrophoresis. Sardaine consists of 34 amino acid residues, and its sequence is: Pr...

journal_title：Journal of biochemistry

authors： Okamoto Y,Kuno K,Motohiro T,Nishi N,Muta E,Ota S

更新日期：1992-02-01 00:00:00

abstract：:Single-chain antibodies (scFv), which can be produced in Escherichia coli cells, have been shown in numerous cases to be active in antigen binding. In the case of the two anti-lysozyme single-chain antibodies, scFvLH and scFvHL, which have the reversed arrangement of the variable domains of the heavy and light chains ...

journal_title：Journal of biochemistry

authors： Merk H,Stiege W,Tsumoto K,Kumagai I,Erdmann VA

更新日期：1999-02-01 00:00:00

abstract：:The effects of intra- and extravesicular calcium and magnesium ions on the hydrolysis of the phosphoenzyme (EP) intermediate formed in the reaction of Ca2+,Mg2+-dependent ATPase of the sarcoplasmic reticulum were investigated. The rate constants of EP hydrolysis were measured under conditions that allowed a single tur...

journal_title：Journal of biochemistry

authors： Daiho T,Takisawa H,Yamamoto T

更新日期：1985-02-01 00:00:00

abstract：:The DNA chain elongation mechanisms of mouse DNA polymerases alpha and beta have been analyzed by using denatured DNA with a (dT)n block at the 3'-end as a template in combination with RNA ((rA)12-20)primer. The (rA)12-20-primed DNA product synthesized by DNA polymerase alpha was 3-5 s in size even after prolonged rea...

journal_title：Journal of biochemistry

authors： Matsukage A,Nishizawa M,Takahashi T,Hozumi T

更新日期：1980-12-01 00:00:00

abstract：:c-Abl non-receptor tyrosine kinase has been implicated in many cellular processes including cell differentiation, stress response and regulating gene transcription. The mechanism by which c-Abl is involved in the regulation of gene transcription remains to be elucidated. In this study, we investigated the functions of...

journal_title：Journal of biochemistry

authors： Jing Y,Wang M,Tang W,Qi T,Gu C,Hao S,Zeng X

更新日期：2007-05-01 00:00:00

abstract：:Three glucosamine-containing sphingoglycolipids were isolated from human lung tissue of a blood group-A subject. They were hexaglycosyl, pentaglycosyl, and tetraglycosyl ceramides. The hexaglycosyl ceramide exhibited blood group-A antigenicity, and the following chemical structure was proposed on the basis of sequenti...

journal_title：Journal of biochemistry

authors： Yoda Y,Makita A,Gasa S

更新日期：1982-02-01 00:00:00

abstract：:The effects of a temperature shift-up and various metabolic inhibitors on the protein synthesis of an endosymbiont isolated from the pea aphid were studied. The syntheses of at least three major polypeptides were stimulated transiently immediately after a temperature shift-up, and treatment with ethanol and heavy meta...

journal_title：Journal of biochemistry

authors： Morioka M,Ishikawa H

更新日期：1992-04-01 00:00:00