A rapid micro-assay method for gelatinolytic activity using tritium-labeled heat-denatured polymeric collagen as a substrate and its application to the detection of enzymes involved in collagen metabolism.

abstract：:The conformational changes of ribonucleic acid induced by heat treatment were studied by measurements of fluorescence polarization, circular dichroism, and ultraviolet absorption. The fluorescence polarization of proflavine covalently bound to the 3'-end of 16S ribosomal RNA of Escherichia coli and that of ethenoadeno...

journal_title：Journal of biochemistry

authors： Kumagai JI,Ohta T,Imahori K

更新日期：1976-03-01 00:00:00

abstract：:The Ca2+-sensitive ATPase activity of rabbit skeletal myofibrils disappeared completely after treatment with a solution containing CDTA, a strong divalent cation chelator, at a low ionic strength. A gel electrophoretic study revealed that all troponin C and about half of myosin light chain 2 were removed from the myof...

journal_title：Journal of biochemistry

authors： Morimoto S,Ohtsuki I

更新日期：1987-02-01 00:00:00

abstract：:Using the mRNA differential display technique and Western blot analysis, the present study demonstrates that induction of KAR2 occurs when misfolded membrane-bound cytochrome P450, mutated in its cytosolically exposed domain, is expressed in Saccharomyces cerevisiae. Using various KAR2 promoter constructs in front of ...

journal_title：Journal of biochemistry

authors： Zimmer T,Ogura A,Ohta A,Takagi M

更新日期：1999-12-01 00:00:00

abstract：:The fructose-1,6-bisphosphate aldolase gene from the thermophilic bacterium, Anoxybacillus gonensis G2, was cloned and sequenced. Nucleotide sequence analysis revealed an open reading frame coding for a 30.9 kDa protein of 286 amino acids. The amino acid sequence shared approximately 80-90% similarity to the Bacillus ...

journal_title：Journal of biochemistry

authors： Ertunga NS,Colak A,Belduz AO,Canakci S,Karaoglu H,Sandalli C

更新日期：2007-06-01 00:00:00

abstract：:Induction of cytosolic aspartate aminotransferase (cAspAT) was observed in rat liver on administration of a high-protein diet, or glucagon and during fasting. The enzyme activity in the liver of rats given 80% protein diet or glucagon injection during starvation increased to 2- to 2.4-fold that in the liver of rats ma...

journal_title：Journal of biochemistry

authors： Horio Y,Tanaka T,Taketoshi M,Uno T,Wada H

更新日期：1988-05-01 00:00:00

abstract：:Apoprotein of electron-transferring flavoprotein (ETF) reacts with FAD as follows: A*<-->A, A+FAD<-->holoETF. Two different forms of apoETF (A* and A) convert into each other and only one of them, A, can associate with FAD [Sato, K. et al. (1991) J. Biochem. 109, 734-740]. In the present study, the reactions between a...

journal_title：Journal of biochemistry

authors： Sato K,Nishina Y,Shiga K

更新日期：1992-12-01 00:00:00

abstract：:Depolymerization of microtubules in the ATP-reassembly buffer permitted the preparation of GDPETNGTP. Incubation of this tubulin fraction at 35 degrees with ATP induced the phosphorylation of E-site GDP into GTP, which was then dephosphorylated during microtubule assembly. Incubation of GDPETNGTP with phosphoenolpyruv...

journal_title：Journal of biochemistry

authors： Kobayashi T,Simizu T

更新日期：1976-06-01 00:00:00

abstract：:Two proteinaceous factors, 15K and 9K proteins, which acted together to stabilize the inactivated yeast F1F0-ATPase-inhibitor complex [Hashimoto, T., et al. (1984) J. Biochem. 95, 131-136] were hardly distinguishable from the sigma and epsilon subunits, respectively, of yeast F1-ATPase by sodium dodecyl sulfate (SDS) ...

journal_title：Journal of biochemistry

authors： Okada Y,Hashimoto T,Yoshida Y,Tagawa K

更新日期：1986-01-01 00:00:00

abstract：:Picolinate binds to a reduced form of D-amino acid oxidase, and the complex formed has a broad absorption band around 600 nm as in the case of the purple intermediate of the enzyme with a substrate. The dissociation constant at 25 degrees C was 35 microns at pH 7.0. The pH dependence (pH 8.3-pH 6.4) of the dissociatio...

journal_title：Journal of biochemistry

authors： Nishina Y,Tojo H,Shiga K

更新日期：1986-03-01 00:00:00

abstract：:The calnexin homologue (Cne1p) of Saccharomyces cerevisiae was expressed in Escherichia coli to evaluate its chaperone function. The chaperone function was examined as to the effects on the suppression of thermal denaturation and the enhancement of refolding, using citrate synthase (CS) as a nonspecific chaperone subs...

journal_title：Journal of biochemistry

authors： Xu X,Kanbara K,Azakami H,Kato A

更新日期：2004-05-01 00:00:00

abstract：:We previously reported that internucleosomal DNA fragmentation, a biochemical feature of apoptosis, was induced spontaneously in high-cell-density culture of adult rat hepatocytes. To understand better the intracellular mechanism of the DNA fragmentation in this system, we have examined the effects of several inhibito...

journal_title：Journal of biochemistry

authors： Maeda S,Suzuki A,Lin KH,Inagaki H,Saito T

更新日期：1995-12-01 00:00:00

abstract：:Incubation of Sarcophaga fat bodies in buffered insect saline resulted in simultaneous activation of multiple defense protein genes [Sugiyama, H. and Natori, S. (1991) Eur. J. Biochem. 200, 495-500]. In this study, we demonstrated that the addition of both adenosine and homocysteine thiolactone to this medium abolishe...

journal_title：Journal of biochemistry

authors： Sugiyama H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:A transition-state analog, gluconolactone, was found to partially quench the protein fluorescence of glucoamylase [EC 3.2.1.3] from Rhizopus niveus. The interaction between gluconolactone and the enzyme was studied statically and kinetically at pH 4.5 in terms of fluorescence change. The dissociation constant Kd of th...

journal_title：Journal of biochemistry

authors： Ohnishi M,Yamashita T,Hiromi K

更新日期：1977-01-01 00:00:00

abstract：:A method for immobilizing oligosaccharides on a TLC plate for immunostaining has been developed. N-Glycolylneuraminic acid (NeuGc)-containing oligosaccharides derived from II3NeuGc-LacCer, IV3NeuGc-nLcOse4Cer, II3NeuGc-GgOse3Cer, and II3(NeuGc)2-LacCer by digestion with our newly isolated endoglycoceramidase (Ito, M. ...

journal_title：Journal of biochemistry

authors： Higashi H,Hirabayashi Y,Ito M,Yamagata T,Matsumoto M,Ueda S,Kato S

更新日期：1987-08-01 00:00:00

abstract：:We identified a protein with a molecular weight of about 44 kDa in an extract of rat regenerating liver. This protein was undetectable in normal, sham-operated, and completely regenerated liver. We also purified this 44-kDa protein from an extract of rat liver remnant after partial hepatectomy. The partial amino acid ...

journal_title：Journal of biochemistry

authors： Tanahashi T,Suzuki M,Itoh N,Mitsui Y

更新日期：1995-08-01 00:00:00

abstract：:The steric course of the enoyl reduction catalyzed by fatty acid synthetase was investigated with the enzymes from bakers' yeast, rat liver and Brevibacterium ammoniagenes. The non-enzymic hydrogen-deuterium exchange of the methylene group of malonyl-CoA was studied by NMR spectroscopy. The half-life period of the met...

journal_title：Journal of biochemistry

authors： Saito K,Kawaguchi A,Seyama Y,Yamakawa T,Okuda S

更新日期：1981-12-01 00:00:00

abstract：:Kinetic parameters, Km and Vmax for the transesterification of oligouridylic acid, (Up)nU greater than p (n=0-4), by RNase A were measured spectrophotometrically at pH 7.0 and 25 degrees C. The kinetic parameters, pKm and log Vmax increased with increase in the chain length (n), and seemed to be almost constant with s...

journal_title：Journal of biochemistry

authors： Irie M,Mikami F,Monma K,Ohgi K,Watanabe H,Yamaguchi R,Nagase H

更新日期：1984-07-01 00:00:00

abstract：:PTF/SNAPc is a multisubunit complex which specifically recognizes the PSEs of small nuclear RNA genes and activates transcription by RNA polymerase II or III. Here we describe the isolation and characterization of genomic clones encoding the human PTFgamma/SNAP43 gene. The gene spans approximately 29 kilobases, and is...

journal_title：Journal of biochemistry

authors： Maeng JH,Yoon JB

更新日期：1998-07-01 00:00:00

abstract：:Ester cerebrosides isolated from whale brain were demonstrated to be a mixture of 6-O-acyl, 2-O-acyl, 4-O-acyl, and 3-O-acyl galactosyl ceramides in that order of content (Yasugi, E., et al. (1982) J. Biochem. 91, 1121-1127). However, the existence of 2-O-acyl and 4-O-acyl galactosyl ceramides has not been reported fo...

journal_title：Journal of biochemistry

authors： Yasugi E,Kasama T,Kojima H,Yamakawa T

更新日期：1983-06-01 00:00:00

abstract：:Prp8 is a splicing factor of 220 kDa originally identified in yeast and is a component of the U5 small nuclear ribonucleoprotein particle. Mouse Prp8 cDNA was cloned and shown to share 62.6 and 68.2% sequence identity with the yeast homologue at the amino acid and nucleotide level, respectively, while it differs by on...

journal_title：Journal of biochemistry

authors： Takahashi A,Muramatsu H,Takagi S,Fujisawa H,Miyake Y,Muramatsu T

更新日期：2001-04-01 00:00:00

abstract：:A novel phenylserine dehydratase [EC 4.2.1.-], which catalyzes the deamination of L-threo-3-phenylserine to yield phenylpyruvate and ammonia, was purified to homogeneity from a crude extract of Pseudomonas pickettii PS22 isolated from soil. The enzyme was a monomer having a molecular mass of about 38 kDa and contained...

journal_title：Journal of biochemistry

authors： Okuda H,Nagata S,Misono H

更新日期：1996-04-01 00:00:00

abstract：:Two cultured cell lines, called Kan-R1 and Kan-R2, were established from rat hepatic cells by in vitro culture with a hepatocarcinogen, 3-methoxy-4-aminoazobenzene, and examined for the gene expression of cytochrome p450 (p450) isoforms, CYP1A1, CYP1A2, CYP2B1, CYP2B2, CYP3A1 and CYP3A2, by the RT-PCR method. It was r...

journal_title：Journal of biochemistry

authors： Degawa M,Namiki M,Yoshimoto N,Makino M,Iwamoto M,Nemoto K,Hashimoto Y

更新日期：2003-06-01 00:00:00

abstract：:The role that interaction with laminin may play in Leishmania donovani infection was investigated. Binding of (125)I-radiolabeled laminin, in a liquid-phase assay, by the parasite was rapid, saturable, specific, reversible, and of high affinity. Using a Western blotting procedure, a 67 kDa laminin-binding protein (LBP...

journal_title：Journal of biochemistry

authors： Bandyopadhyay K,Karmakar S,Ghosh A,Das PK

更新日期：2001-07-01 00:00:00

abstract：:The effects of unsaturated fatty acids on the activities of peroxisomal enzymes of Tetrahymena pyriformis were investigated. When saturated fatty acids and the corresponding unsaturated fatty acids (C18) were added to the culture medium at 0.05%, the activities of peroxisomal enzymes [fatty acyl-CoA oxidase (FAO), car...

journal_title：Journal of biochemistry

authors： Gotoh K,Takei M,Watanabe T,Suga T

更新日期：1986-10-01 00:00:00

abstract：:PKNalpha is a fatty acid- and Rho-activated serine/threonine protein kinase having a catalytic domain homologous to members of the protein kinase C family. Recently it was reported that PKNalpha is involved in the p38 mitogen-activated protein kinase (MAPK) signaling pathway. To date, however, how PKNalpha regulates t...

journal_title：Journal of biochemistry

authors： Takahashi M,Gotoh Y,Isagawa T,Nishimura T,Goyama E,Kim HS,Mukai H,Ono Y

更新日期：2003-02-01 00:00:00

abstract：:The nucleotide sequences of 5S rRNA from seven denitrifying bacteria have been determined. Based on these sequences and those reported in the literature (including two denitrifiers), a phylogenic tree of 104 eubacterial 5S rRNA sequences has been constructed to establish the position of the denitrifying bacteria. Thes...

journal_title：Journal of biochemistry

authors： Ohkubo S,Iwasaki H,Hori H,Osawa S

更新日期：1986-11-01 00:00:00

abstract：:Serologically active D-arabino-D-mannas ([alpha]D, +82 degrees approximately 89 degrees; ratio of D-arabinose to D-mannose, 1-2:1) were isolated from the soluble fraction of disintegrated cells of M. tuberculosis, M. smegmatis, and several other Mycobacterium species. These arabinomannans had similar structures, consi...

journal_title：Journal of biochemistry

authors： Misaki A,Azuma I,Yamamura Y

更新日期：1977-12-01 00:00:00

abstract：:beta1,4-galactosyltransferase II (beta1,4GalT II) is one of the enzymes transferring galactose to the terminal N-acetylglucosamine of complex-type N-glycans. Previously, we have reported that beta1,4GalT II overexpression increased cisplatin-induced HeLa cell apoptosis. However, the mechanisms of its expression regula...

journal_title：Journal of biochemistry

authors： Zhou J,Wei Y,Liu D,Ge X,Zhou F,Jiang XY,Gu J

更新日期：2008-04-01 00:00:00

abstract：:The assembly of newly synthesized histones into chromatin during replication of MH-134SC cells was studied. Cells pulse-labeled with iododeoxyuridine and [3H]lysine were mixed with an equivalent number of normal cells labeled with [14C]lysine. Nuclease chromatin obtained from pooled cells was fractionated by buoyant-d...

journal_title：Journal of biochemistry

authors： Senshu T,Fukuda M,Ohashi M

更新日期：1978-10-01 00:00:00

abstract：:Hepatic peroxisomes and mitochondria from 20-day-old chick embryo were separated by sucrose density gradient centrifugation and the characteristics of carnitine acyltransferases in these organelles were studied. The carnitine acyltransferase activities in peroxisomes were increased markedly by the treatment of chick e...

journal_title：Journal of biochemistry

authors： Ishii S,Ishii H,Suga T

更新日期：1985-09-01 00:00:00