Evolutionary relationship of denitrifying bacteria as deduced from 5S rRNA sequences.

abstract：:The Ca2+-sensitive ATPase activity of rabbit skeletal myofibrils disappeared completely after treatment with a solution containing CDTA, a strong divalent cation chelator, at a low ionic strength. A gel electrophoretic study revealed that all troponin C and about half of myosin light chain 2 were removed from the myof...

journal_title：Journal of biochemistry

authors： Morimoto S,Ohtsuki I

更新日期：1987-02-01 00:00:00

abstract：:Comparative studies of the unfolding equilibria of two homologous proteins, bovine alpha-lactalbumin and hen lysozyme, induced by treatment with guanidine hydrochloride have been made by analysis of the peptide and the aromatic circular dichroism spectra. The effect of the specific binding of Ca2+ ion by the former pr...

journal_title：Journal of biochemistry

authors： Ikeguchi M,Kuwajima K,Sugai S

更新日期：1986-04-01 00:00:00

abstract：:A novel esterase was found in Pseudomonas fluorescens cells and purified to homogeneity as determined by polyacrylamide gel electrophoresis. The esterase was extracted from the cells by freeze-thawing and hypotonic treatment. Purification was achieved by ammonium sulfate precipitation, followed by successive chromatog...

journal_title：Journal of biochemistry

authors： Nakagawa A,Tsujita T,Okuda H

更新日期：1984-04-01 00:00:00

abstract：:1. Z-line constituents contributing to the formation of the contraction bands in chicken myofibrils were investigated by using the rabbit calcium-activated factor (CAF). 2. CAF digestion hampered the formation of the contraction bands and increased the amount of soluble proteins which are apparently derived from the Z...

journal_title：Journal of biochemistry

authors： Muguruma M,Muguruma Y,Fukazawa T

更新日期：1980-07-01 00:00:00

abstract：:A novel fucose-containing neutral glycosphingolipid (GL-5) was purified from the eggs of the sea urchin, Hemicentrotus pulcherrimus. The chemical structure was determined to be Fuc alpha 1-3GalNAc beta 1-4(Fuc alpha 1-3)GlcNAc beta 1-4Glc beta 1-1Cer by methylation analysis, partial acid hydrolysis, fast atom bombardm...

journal_title：Journal of biochemistry

authors： Kubo H,Jiang GJ,Irie A,Suzuki M,Inagaki F,Hoshi M

更新日期：1992-08-01 00:00:00

abstract：:1. A major glucoamylase [EC 3.2.1.3] of Aspergillus saitoi was purified by ultrafiltration followed by successive chromatography on DEAE-Sephadex, Ultrogel AcA 44 and SP-Sephadex. The purification achieved was 23-fold from crude extract with a yield of 21%. The purified enzyme, named Gluc M1, was proved homogeneous as...

journal_title：Journal of biochemistry

authors： Takahashi T,Inokuchi N,Irie M

更新日期：1981-01-01 00:00:00

abstract：:High mobility group (HMG) protein 1 contains two DNA binding motifs, called HMG1/2-boxes, linked with a linker region. The functional relationships between the two boxes and the mechanism of involvement of the linker region for effective binding of HMG1 were examined. The binding analyses of truncated HMG1 peptides wi...

journal_title：Journal of biochemistry

authors： Saito K,Kikuchi T,Shirakawa H,Yoshida M

更新日期：1999-02-01 00:00:00

abstract：:We previously isolated a Ca2+-binding protein from a ciliate, Tetrahymena, and designated it as TCBP (Tetrahymena Ca2+-binding protein). The present paper reports that TCBP, which has two high affinity Ca2+-binding sites (Kd=4.6 X 10(-6) M), could activate porcine brain cyclic nucleotide phosphodiesterase at a concent...

journal_title：Journal of biochemistry

authors： Suzuki Y,Nagao S,Abe K,Hirabayashi T,Watanabe Y

更新日期：1981-01-01 00:00:00

abstract：:A simple and rapid method to remove myosin light chain phosphatase from gizzard myosin using hydroxyapatite chromatography was developed. Stable phosphorylated myosin without light chain phosphatase and kinase was also prepared by the same procedure. A convenient method using the urea gel system to detect minor contam...

journal_title：Journal of biochemistry

authors： Nakamura S,Nonomura Y

更新日期：1984-08-01 00:00:00

abstract：:Selected biochemical properties, including the charge heterodispersity profile and carbohydrate specificity, of bovine galectin-1 were determined in detail. The lectin was purified through an improved purification protocol that yielded 35-40 mg/kg of wet tissue with a specific activity of 1.7-2 x 10(4) mg-1.ml. The ga...

journal_title：Journal of biochemistry

authors： Ahmed H,Fink NE,Pohl J,Vasta GR

更新日期：1996-11-01 00:00:00

abstract：:Association of sulphatide with influenza A virus (IAV) haemagglutinin (HA) delivered to the cell surface promotes progeny virus production. However, it is not known whether there is direct binding of HA to sulphatide. In this study, we found that recombinant HA, which was produced by a baculovirus protein expression s...

journal_title：Journal of biochemistry

authors： Takahashi T,Satoh H,Takaguchi M,Takafuji S,Yokoyama H,Fujii S,Suzuki T

更新日期：2010-04-01 00:00:00

abstract：:Structural studies were carried out on the teichoic acids in cell walls of Listeria monocytogenes serotypes 3a, 4b, 4f, 6, and 7. The structure of the dephosphorylated repeating units, obtained by treatment with 46% hydrogen fluoride or alkaline hydrolysis, was examined by methylation analysis, acetolysis, and 1H-NMR ...

journal_title：Journal of biochemistry

authors： Uchikawa K,Sekikawa I,Azuma I

更新日期：1986-02-01 00:00:00

abstract：:We previously reported that internucleosomal DNA fragmentation, a biochemical feature of apoptosis, was induced spontaneously in high-cell-density culture of adult rat hepatocytes. To understand better the intracellular mechanism of the DNA fragmentation in this system, we have examined the effects of several inhibito...

journal_title：Journal of biochemistry

authors： Maeda S,Suzuki A,Lin KH,Inagaki H,Saito T

更新日期：1995-12-01 00:00:00

abstract：:Tissue remodelling and regeneration in various pathophysiological conditions (e.g. the processes of development, pregnancy, inflammation, wound healing, tissue regeneration, tumor growth, etc.) require angiogenesis, a dynamically coordinated response to stimuli from the extracellular microenvironment. During angiogeni...

journal_title：Journal of biochemistry

authors： Sakaue T,Maekawa M,Nakayama H,Higashiyama S

更新日期：2017-10-01 00:00:00

abstract：:Beta-N-Acetylgalactosaminidase [EC 3.2.1.53] was purified to homogeneity from the culture media of Bacillus sp. AT173-1. The enzyme has a molecular weight of 48,000 as estimated by SDS-PAGE under reducing conditions and an isoelectric point of 4.3. The enzyme requires dithiothreitol as an activator and is most active ...

journal_title：Journal of biochemistry

authors： Tanaka A,Ozaki S

更新日期：1997-08-01 00:00:00

abstract：:Serum at 5 to 10% is required for maintenance of functional adult rat hepatocytes in primary culture. One effect of the serum is to induce attachment and spreading of hepatocytes on plates as monolayers. Another role is to maintain cell viability for over 2 days. For the first effect, serum could be replaced completel...

journal_title：Journal of biochemistry

authors： Horiuti Y,Nakamura T,Ichihara A

更新日期：1982-12-01 00:00:00

abstract：:The isotypes of sarcoplasmic Ca2+ binding protein (SCP) were purified from shrimp tail muscle. SCP exists in a dimeric form. One sample of shrimp contained only alpha A chain, whereas another contained alpha B and beta chains, and a heterodimer of alpha B beta which was not analyzed precisely. The amino acid sequences...

journal_title：Journal of biochemistry

authors： Takagi T,Konishi K

更新日期：1984-06-01 00:00:00

abstract：:The effects of freezing on Alcaligenes sp. nitrite reductase [nitric-oxide: ferricytochrome c oxidoreductase, EC 1.7.2.1] dissolved in sodium phosphate (pH 7.2) were investigated. The nitrite reductase was gradually activated with time in the frozen state, resulting in an increase in its activity of 2.5-4.5 times. The...

journal_title：Journal of biochemistry

authors： Masuko M,Iwasaki H,Sakurai T,Suzuki S,Nakahara A

更新日期：1985-11-01 00:00:00

abstract：:Cilia and flagella of eukaryotic cells are evolutionarily conserved organelles with a microtubule-based axoneme as a scaffold. To fulfil their functions in cellular motility, sensory reception and developmental signalling, cilia contain unique proteins, such as receptors and ion channels. The assembly and maintenance ...

journal_title：Journal of biochemistry

authors： Nakayama K,Katoh Y

更新日期：2018-03-01 00:00:00

abstract：:His397 was replaced with alanine by site-directed mutagenesis of the cloned PRC1 gene in order to confirm the role of this residue in the proton-relay system of carboxypeptidase Y (CPY). The expressed and purified H397A showed a CD spectrum almost identical to that of the wild type enzyme, but its heat stability and c...

journal_title：Journal of biochemistry

authors： Jung G,Ueno H,Hayashi R

更新日期：1998-08-01 00:00:00

abstract：:Raft is a mobile membrane subdomain enriched in sphingolipid and cholesterol and also various signaling molecules. Previous observation suggested that brain-derived rafts contain tubulin but that rafts of non-neural origin do not. We hypothesized that SCG10, one of the neuronal growth-associated proteins (nGAPs), migh...

journal_title：Journal of biochemistry

authors： Maekawa S,Morii H,Kumanogoh H,Sano M,Naruse Y,Sokawa Y,Mori N

更新日期：2001-05-01 00:00:00

abstract：:The intracellular distribution of 10Sa RNA in Escherichia coli was investigated in cell extracts. Northern hybridization revealed that a large fraction of 10Sa RNA cosediments with 70S ribosomes. When 70S ribosomes were dissociated into 50S and 30S subunits in the presence of low levels of Mg2+ ions, almost all of the...

journal_title：Journal of biochemistry

authors： Komine Y,Kitabatake M,Inokuchi H

更新日期：1996-03-01 00:00:00

abstract：:We have recently demonstrated the localization of associated m-calpain and calpastatin in the endoplasmic reticulum (ER) of bovine pulmonary artery smooth muscle. Herein, we sought to determine the role of m-calpain on calcium-dependent proteolytic cleavage of Na(+)/Ca(2+) exchanger (NCX) in the ER. Treatment of the E...

journal_title：Journal of biochemistry

authors： Samanta K,Kar P,Chakraborti T,Chakraborti S

更新日期：2010-02-01 00:00:00

abstract：:The effects of D-glucose anomers on the germination of dormant spores of Bacillus megaterium QM B1551 were studied, alpha-D-Glucose (1 mM) slightly initiated the germination of the dormant spores during 10 min incubation at 37 degrees C, while about 60% of the dormant spores became germinated with beta-D-glucose (1 mM...

journal_title：Journal of biochemistry

authors： Miwa I,Ishihara H,Fujii H,Nagano H,Okuda J

更新日期：1979-01-01 00:00:00

abstract：:Translesion DNA synthesis (TLS) involves PCNA mono-ubiquitination and TLS DNA polymerases (pols). Recent evidence has shown that the mono-ubiquitination is induced not only by DNA damage but also by other factors that induce stalling of the DNA replication fork. We studied the effect of spontaneous DNA replication err...

journal_title：Journal of biochemistry

authors： Suzuki M,Niimi A,Limsirichaikul S,Tomida S,Miao Huang Q,Izuta S,Usukura J,Itoh Y,Hishida T,Akashi T,Nakagawa Y,Kikuchi A,Pavlov Y,Murate T,Takahashi T

更新日期：2009-07-01 00:00:00

abstract：:ATP-dependent enzymes were investigated as to the stringency of their ATP requirement. For all the enzymes examined except firefly luciferase (including hexokinase, polynucleotide kinase, T4 DNA ligase, and T4 RNA ligase) ATP could be replaced with dATP, contradicting previous data. Considering the replaceable nucleot...

journal_title：Journal of biochemistry

authors： Kinoshita Y,Nishigaki K

更新日期：1997-07-01 00:00:00

abstract：:Aorta smooth muscle myosin contains two kinds of 17-kDa essential light chain, LC17nm (nonmuscle-type) and LC17gi (gizzard-type) [Hasegawa, Y., Ueda, Y., Watanabe, M., & Morita, F. (1992) J. Biochem. 111, 798-803]. The LC17 isoforms were released from porcine aorta myosin by incubation at 46 degrees C. The rate of rel...

journal_title：Journal of biochemistry

authors： Hasegawa Y,Morita F

更新日期：1992-06-01 00:00:00

abstract：:The heavy chain of myosin subfragment-1 prepared by chymotrypsin treatment had a molecular weight of about 96 K. It was split into 26 K, 50K, and 21 K fragments on trypsin treatment. The effect of actin binding on the susceptibilities of the junctions between 26 K and 50 K and between 50 K and 21 K, and on that of alk...

journal_title：Journal of biochemistry

authors： Yamamoto K,Sekine T

更新日期：1979-12-01 00:00:00

abstract：:A novel glycosphingolipid (SEGLx) has been isolated from the plerocercoids of a tapeworm, Spirometra erinacei. From the results of compositional analysis, methylation analysis, exoglycosidase hydrolysis, acid hydrolysis, fast atom bombardment mass spectrometry, and proton nuclear magnetic resonance (NMR) analysis, its...

journal_title：Journal of biochemistry

authors： Kawakami Y,Nakamura K,Kojima H,Suzuki M,Inagaki F,Suzuki A,Sonoki S,Uchida A,Murata Y,Tamai Y

更新日期：1993-11-01 00:00:00

abstract：:The heparin-induced self-aggregation behaviours of four repeat peptides (R1-R4) in an acidic solution (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and compared with those in a neutral solution (pH = 7.5). In contrast with the self-aggregation-resistive behaviours of the R1 and ...

journal_title：Journal of biochemistry

authors： Mizushima F,Minoura K,Tomoo K,Sumida M,Taniguchi T,Ishida T

更新日期：2007-07-01 00:00:00