Effects of unsaturated fatty acids on the peroxisomal enzyme activities of Tetrahymena pyriformis.

abstract：:The interaction between pullulanase from Klebsiella pneumoniae and alpha-, beta-, and gamma-cyclodextrins and 6-O-alpha-glucosyl-alpha-cyclodextrin and 6-O-alpha-glucosyl-beta-cyclodextrin was examined by means of inhibition studies of the enzyme activity, UV difference spectroscopy, and flow calorimetry. All the abov...

journal_title：Journal of biochemistry

authors： Iwamoto H,Ohmori M,Ohno M,Hirose J,Hiromi K,Fukada H,Takahashi K,Hashimoto H,Sakai S

更新日期：1993-01-01 00:00:00

abstract：:Mannan, a ligand for the mannose/N-acetylglucosamine (GlcNAc) receptor, induces suppression of oxygen consumption and increases glucose production in the perfused rat liver, and repeated infusion of mannan causes desensitization of the responses. In this study, we examined whether activation of Kupffer cells by endoto...

journal_title：Journal of biochemistry

authors： Kimura K,Hamada M,Moriyama M,Kannan Y,Shiota M,Sakurada K,Musashi M,Sugano T

更新日期：1996-09-01 00:00:00

abstract：:Chemical modifications by photooxidation in the presence of rose bengal (RB) and with tetranitromethane (TNM) were carried out to elucidate the amino acid residues involved in the active site of inorganic pyrophosphatase (pyrophosphate phosphohydrolase) [EC 3.6.1.1] from Escherichia coli Q13. The photooxidation caused...

journal_title：Journal of biochemistry

authors： Samejima T,Tamagawa Y,Kondo Y,Hachimori A,Kaji H,Takeda A,Shiroya Y

更新日期：1988-05-01 00:00:00

abstract：:Isoleucyl-tRNA formation and isoleucine-dependent PPi-ATP exchange catalyzed by purified isoleucyl-tRNA synthetase [EC 6.1.1.5] of Escherichia coli were studied in the presence of various amounts of either Mg2+, Ca2+, Fe2+, Ni2+, or Cu2+. In the presence of Mg2+, isoleucine-dependent PPi-ATP exchange was observed in p...

journal_title：Journal of biochemistry

authors： Takeda Y,Ohnishi T,Ogiso Y

更新日期：1976-09-01 00:00:00

abstract：:The internal structure of the recombinant yeast-derived human hepatitis B virus surface antigen vaccine particle was investigated by small-angle neutron scattering using the contrast variation method. Data were collected in aqueous buffer solutions containing 0, 40, 60, and 100% D2O in the q range of 0.005 to 0.2 A-1 ...

journal_title：Journal of biochemistry

authors： Sato M,Sato-Miyamoto Y,Kameyama K,Ishikawa N,Imai M,Ito Y,Takagi T

更新日期：1995-12-01 00:00:00

abstract：:When the membrane fraction of Saccharomyces carlsbergensis was incubated with radiolabeled isopentenyl diphosphate in the presence of farnesyl diphosphate and Mg2+, phosphorylated and free long-chain polyprenols were formed. The reaction was inhibited by EDTA and heavy metal cations. A series of non-ionic detergents w...

journal_title：Journal of biochemistry

authors： Bukhtiyarov YE,Shabalin YA,Kulaev IS

更新日期：1993-06-01 00:00:00

abstract：:Myosin was isolated from leucocytes in horse arterial blood by the same procedures used for the isolation of myosin from skeletal muscle. The Ca2+-, EDTA-, and Mg2+-ATPase [EC 3.6.1.3] activities of the protein was 0.148, 0.147, and 0.001 mumoles/min/mg, respectively, in 0.5 M KCl at pH 7.0 and 25 degrees. The Ca2+-AT...

journal_title：Journal of biochemistry

authors： Takeuchi K,Shibata N,Senda N

更新日期：1975-07-01 00:00:00

abstract：:Thyrotropin releasing hormone (TRH) caused significant breakdown of phosphatidylinositol 4,5-bisphosphate (PIP2) in GH3 cells, but vasoactive intestinal peptide (VIP) did not. However, VIP enhanced the TRH-induced hydrolysis of PIP2, the conversion of phosphatidylinositol 4-phosphate (PIP) to PIP2 and the accumulation...

journal_title：Journal of biochemistry

authors： Suzuki R,Takenawa T

更新日期：1987-09-01 00:00:00

abstract：:It was previously found that rabbit serum contains a growth-inhibitory substance for a tumorigenic rat liver cell line RSV-BRL. In the present study, the growth inhibitor was purified from normal rabbit serum to show a homogeneous protein band with a molecular weight (Mr) of 56 k on SDS-polyacrylamide gel electrophore...

journal_title：Journal of biochemistry

authors： Kimura T,Miyazaki K,Mashima K,Yamashita J,Horio T,Kakuno T

更新日期：1991-09-01 00:00:00

abstract：:When the body temperature of rats was brought to 42 degrees C, four heat-shock proteins, with molecular weights of 70,000, 71,000, 85,000, and 100,000 (hsp 70, hsp 71, hsp 85, and hsp 100, respectively), were induced in various tissues of the rats. The hsp 70 was strongly induced by hyperthermia, and its accumulation ...

journal_title：Journal of biochemistry

authors： Fujio N,Hatayama T,Kinoshita H,Yukioka M

更新日期：1987-01-01 00:00:00

abstract：:7 beta-Hydroxysteroid dehydrogenase (7 beta-HSD) was produced by Ruminococcus sp. PO1-3 obtained from among human intestinal bacteria. The enzyme was purified from a crude extract by ammonium sulfate fractionation, and Butyl-Toyopearl 650M, Sephadex G-150, Matrex Red A and Octyl-Sepharose chromatographies. The purifie...

journal_title：Journal of biochemistry

authors： Akao T,Akao T,Kobashi K

更新日期：1987-09-01 00:00:00

abstract：:Pig heart phosphoprotein phosphatase [phosphoprotein phosphophydrolase, EC 3.1.3.16] of Mr 224,000 was dissociated by gel-filtration on Sephacryl S-300, into an active subunit (alpha subunit) of Mr 31,000 and inactive subunits of higher molecular weight in the presence of 6 M urea. After the removal of urea, these sub...

journal_title：Journal of biochemistry

authors： Imazu M,Imaoka T,Usui H,Kinohara N,Takeda M

更新日期：1981-09-01 00:00:00

abstract：:It has been demonstrated that the GM2 ganglioside cannot be cleaved by exo-beta-N-acetylhexosaminidases isolated from molds or bacterial sources. Here, a novel GM2 ganglioside-degrading enzyme was found in cells of Nocardia sp. This enzyme releases free fatty acids from the GM2 ganglioside. The chemical structure of t...

journal_title：Journal of biochemistry

authors： Hirabayashi Y,Kimura M,Matsumoto M,Yamamoto K,Kadowaki S,Tochikura T

更新日期：1988-01-01 00:00:00

abstract：:A method was developed to obtain heavy meromyosin (HMM) from the tryptic digest of skipjack tuna dorsal myosin. The tuna HMM thus obtained was shown to be homogeneous on gel filtration-gel electrophoresis, and on ultracentrifugation. The sedimentation constant (S20,w) was estimated to be 6.1S for tuna HMM. The ATPase ...

journal_title：Journal of biochemistry

authors： Kimura I,Arai K,Watanabe S

更新日期：1979-12-01 00:00:00

abstract：:Two DFP-sensitive alkaline proteinases with strong esterase activity toward Ac-(Ala)3-OMe, designated as alkaline serine proteinases D and E, were purified pronase, a protease mixture from St. griseus K-1. Each was shown to be homogeneous by acrylamide disc gel electrophoresis. The molecular weights of these enzymes w...

journal_title：Journal of biochemistry

authors： Narahashi Y,Yoda K

更新日期：1977-03-01 00:00:00

abstract：:The three-dimensional structure of rat-liver acyl-CoA oxidase-II (ACO-II) in a complex with a C12-fatty acid was solved by the molecular replacement method based on the uncomplexed ACO-II structure. The crystalline form of the complex was obtained by cocrystallization of ACO-II with dodecanoyl-CoA. The crystalline com...

journal_title：Journal of biochemistry

authors： Tokuoka K,Nakajima Y,Hirotsu K,Miyahara I,Nishina Y,Shiga K,Tamaoki H,Setoyama C,Tojo H,Miura R

更新日期：2006-04-01 00:00:00

abstract：:The electron transport system coupled to the oxidation of methylamine in Pseudomonas AM1 was investigated by reconstituting it from the highly purified components. A mixture of methylamine dehydrogenase, cytochrome cH and cytochrome c oxidase (= cytochrome aa3) actively oxidized methylamine (161 mol of O2 consumed/mol...

journal_title：Journal of biochemistry

authors： Fukumori Y,Yamanaka T

更新日期：1987-02-01 00:00:00

abstract：:The changes in the cellular concentrations of ATP, ADP, and AMP and in oxidative phosphorylation of mitochondria were investigated in the remaining liver of partially hepatectomized rabbits. The energy charge (defined as half of the average number of anhydride-bonded phosphate groups per adenosine moiety) of the liver...

journal_title：Journal of biochemistry

authors： Kamiyama Y,Ozawa K,Honjo I

更新日期：1976-10-01 00:00:00

abstract：:The kinetic specificities of BPN' and Carlsberg subtilisins [EC 3.4.21.14] were examined with various nucleus-substituted derivatives of Nalpha-acetylated aromatic amino acid methyl esters for mapping their hydrophobic binding sites in comparison with that of alpha-chymotrypsin. The Carlsberg enzyme was generally much...

journal_title：Journal of biochemistry

authors： Karasaki Y,Ohno M

更新日期：1978-09-01 00:00:00

abstract：:Proteins in the brush border membrane (BBM) of the midgut binding to the insecticidal Cry1Ac toxin from Bacillus thuringiensis were investigated to examine the lower sensitivity of Bombyx mori to Cry1Ac, and new aminopeptidase N that bound to Cry1Ac was discovered. DEAE chromatography of Triton X-100-soluble BBM prote...

journal_title：Journal of biochemistry

authors： Shitomi Y,Hayakawa T,Hossain DM,Higuchi M,Miyamoto K,Nakanishi K,Sato R,Hori H

更新日期：2006-02-01 00:00:00

abstract：:Covalently closed-circular, superhelical DNAs, including viral DNAs, bacterial plasmid DNAs, and bacteriophage replicative-form DNA, were treated with a small amount of Haemophilus gallinarum DNA-relaxing enzyme to generate incompletely relaxed DNA molecules. Each sample consisted of a set of closed-circular DNA molec...

journal_title：Journal of biochemistry

authors： Shishido K

更新日期：1979-09-01 00:00:00

abstract：:Controlled chymotryptic digestion of the 165,000 dalton component of the M-line of rabbit skeletal muscle, followed by Biogel P-150 chromatography of the digest, has led to the isolation of a homogeneous 100,000 dalton species. This fragment was found, by both sedimentation equilibrium and gel filtration chromatograph...

journal_title：Journal of biochemistry

authors： Mani RS,Kay CM

更新日期：1979-12-01 00:00:00

abstract：:The effects of various compounds on ascorbate-2-sulfate sulfohydrolase and arylsulfatase (EC 3.1.6.1) activities in the copurified preparation from the liver of Charonia lampas were investigated. The former activity was competively inhibited by inorganic phosphate and sulfate. The latter was not affected by sulfate....

journal_title：Journal of biochemistry

authors： Hatanaka H,Ogawa Y,Egami F

更新日期：1975-04-01 00:00:00

abstract：:We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted into liposomes comprising Salmonella minnesota R595 lipopolysaccharides, and fusion of spleen cells with a mouse myeloma cell...

journal_title：Journal of biochemistry

authors： Watarai S,Kushi Y,Shigeto R,Misawa N,Eishi Y,Handa S,Yasuda T

更新日期：1995-05-01 00:00:00

abstract：:Highly purified recombinant human tumor necrosis factor (TNF) (molecular mass determined as 17 kilodaltons (kDa) by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and as 36 kDa by Sephadex G-100 gel chromatography) was labeled with 125I to a specific activity of 5 microCi/micrograms without appreciable loss...

journal_title：Journal of biochemistry

authors： Yoshie O,Tada K,Ishida N

更新日期：1986-09-01 00:00:00

abstract：:We have unexpectedly found that Streptomyces subtilisin inhibitor (SSI) and some other similar serine protease inhibitors produced by Streptomycetes strongly inhibit Streptomyces griseus metallo-endopeptidase II (SGMP II) [Kajiwara, K. et al. (1991) J. Biochem. 110, 350-354]. In order to elucidate the mode of their un...

journal_title：Journal of biochemistry

authors： Kumazaki T,Kajiwara K,Kojima S,Miura K,Ishii S

更新日期：1993-10-01 00:00:00

abstract：:The vitamin A derivative, retinoic acid (RA) has various biological effects in mammalian cells and tissues. It is well known that RA induces differentiation of leukemia cells and inhibits cell growth. There are two pathways for RA action; one via RA nuclear receptors (RARs), and one via acylation of proteins by RA (re...

journal_title：Journal of biochemistry

authors： Kubo Y,Ohba T,Takahashi N

更新日期：2008-09-01 00:00:00

abstract：:The catalytic properties of a new type of dihydropteridine reductase, NADPH-specific dihydropteridine reductase [EC 1.6.99.10], from bovine liver, were studied and compared with those of the previously characterized enzyme, NADH-specific dihydropteridine reductase [EC 1.6.99.7]. With quinonoid-dihydro-6-methylpterin, ...

journal_title：Journal of biochemistry

authors： Nakanishi N,Hasegawa H,Akino M,Yamada S

更新日期：1986-03-01 00:00:00

abstract：:To gain insight into the mechanism by which the sequence at the rne-dependent site of substrate RNA affects the substrate specificity of Escherichia coli RNase E, we performed kinetic analysis of the cleavage of precursor M1 RNA molecules containing various sequences at the rne-dependent site by the N-terminal catalyt...

journal_title：Journal of biochemistry

authors： Kim KS,Sim S,Ko JH,Cho B,Lee Y

更新日期：2004-11-01 00:00:00

abstract：:Monkey (Macaca fuscata) mononuclear leukocytes were stimulated to produce thromboplastin (tissue factor) upon exposure to lipopolysaccharide, LPS. The stimulation was dose-related in the concentration range of 10(-5) to 10(-1) micrograms/ml of LPS. Lipid A portion of the LPS molecule was essential to induce the leukoc...

journal_title：Journal of biochemistry

authors： Nakamura S,Gotoh S,Takenaka O,Takahashi K

更新日期：1985-06-01 00:00:00