Heavy meromyosin from skipjack tuna, Euthynus pelamis. Preparation and enzymic properties.

abstract：:beta-Hydroxyisovalerylshikonin (beta-HIVS), which was isolated from the plant, Lithospermium radix, inhibited the growth of various lines of cancer cells derived from human solid tumors at low concentrations between 10(-8) and 10(-6) M. When HL-60 cells were treated with 10(-6) M beta-HIVS for 3 h, characteristic feat...

journal_title：Journal of biochemistry

authors： Hashimoto S,Xu M,Masuda Y,Aiuchi T,Nakajo S,Cao J,Miyakoshi M,Ida Y,Nakaya K

更新日期：1999-01-01 00:00:00

abstract：:Incubation of Sarcophaga fat bodies in buffered insect saline resulted in simultaneous activation of multiple defense protein genes [Sugiyama, H. and Natori, S. (1991) Eur. J. Biochem. 200, 495-500]. In this study, we demonstrated that the addition of both adenosine and homocysteine thiolactone to this medium abolishe...

journal_title：Journal of biochemistry

authors： Sugiyama H,Natori S

更新日期：1996-02-01 00:00:00

abstract：:We obtained two kinds of vesicle preparations which were of different density from the same gastric mucosae of hogs stimulated with food before slaughter. Both kinds contained H+,K+-ATPase. The light vesicle preparation differed from the heavy vesicle preparation as follows: the KCl permeability across the membrane of...

journal_title：Journal of biochemistry

authors： Asano S,Iino T,Tabuchi Y,Takeguchi N

更新日期：1988-04-01 00:00:00

abstract：:Human, bovine, and mouse C1q, a subcomponent of the first complement component, were purified, and both globular (GF) and collagen-like fragments (CLF) were isolated from human and bovine C1q. Antisera were produced in rabbits with these C1q or fragments, and F(ab')2 of immunoglobulin G (IgG) was purified from the ant...

journal_title：Journal of biochemistry

authors： Yonemasu K,Sasaki T,Shimizu A

更新日期：1983-04-01 00:00:00

abstract：:The flavoenzyme medium-chain acyl-CoA dehydrogenase (MCAD) eliminates the alpha-proton of the substrate analog, 3-thiaoctanoyl-CoA (3S-C8-CoA), to form a charge-transfer complex with deprotonated 3S-C8-CoA. This complex can simulate the metastable reaction intermediate immediately after the alpha-proton elimination of...

journal_title：Journal of biochemistry

authors： Satoh A,Nakajima Y,Miyahara I,Hirotsu K,Tanaka T,Nishina Y,Shiga K,Tamaoki H,Setoyama C,Miura R

更新日期：2003-08-01 00:00:00

abstract：:The immunological properties of acid proteinases from rat spleen, two types of cathepsin D and a cathepsin E-like enzyme, were examined. The rabbit antiserum was prepared against the major form of cathepsin D (cathepsin D-I) from rat spleen. The antiserum quantitatively precipitated the enzyme activity from the purifi...

journal_title：Journal of biochemistry

authors： Yamamoto K,Kamata O,Katsuda N,Kato K

更新日期：1980-02-01 00:00:00

abstract：:Knockout mice lacking glycosyltransferases or sulfotransferases provide unequivocal evidence that the carbohydrate moieties of glycoproteins, glycolipids, and proteoglycans play essential roles in various biological phenomena such as development, the immune response, and tissue functions. Examples of abnormalities of ...

journal_title：Journal of biochemistry

authors： Muramatsu T

更新日期：2000-02-01 00:00:00

abstract：:The ascorbate oxidase obtained from a microorganism, Acremonium sp. HI-25 (molecular weight, 80 kDa; monomeric protein), was studied with respect to atomic absorption, EPR, absorption spectra, circular dichroism (CD) spectra, and steady-state kinetics. The enzyme was found to be a multicopper protein, containing four ...

journal_title：Journal of biochemistry

authors： Hirose J,Sakurai T,Imamura K,Watanabe H,Iwamoto H,Hiromi K,Itoh H,Shin T,Murao S

更新日期：1994-05-01 00:00:00

abstract：:Proteoheteroglycan (PHG) was prepared from Neurospora crassa cells by extraction with hot water followed by cetyltrimethylammoniumbromide fractionation. The polymer was purified by DEAE-cellulose chromatography followed by gel filtrations. The PHG was fractionated into five subfractions containing carbohydrate (65-88%...

journal_title：Journal of biochemistry

authors： Nakajima T,Yoshida M,Hiura N,Matsuda K

更新日期：1984-10-01 00:00:00

abstract：:We investigated the expression and functions of circular RNA (circRNA) circNINL and miR-921 in breast cancer (BC) in this study. We found that the expression of circNINL increased while the expression of miR-921 decreased in BC tissues and cell lines, and their anomalous expressions were associated with malignant feat...

journal_title：Journal of biochemistry

authors： Xu C,Yu H,Yin X,Zhang J,Liu C,Qi H,Liu P

更新日期：2021-01-21 00:00:00

abstract：:Deleting the gene for small RNA GcvB in Esherichia coli was found to increase the sensitivity to several aminoglycoside antibiotics, such as neomycin, streptomycin, kanamycin, kasugamycin and spectinomycin, at low concentrations. GcvB, conserved in gram-negative enteric bacteria, is known to negatively control the exp...

journal_title：Journal of biochemistry

authors： Muto A,Goto S,Kurita D,Ushida C,Himeno H

更新日期：2020-11-10 00:00:00

abstract：:Induction of cytosolic aspartate aminotransferase (cAspAT) was observed in rat liver on administration of a high-protein diet, or glucagon and during fasting. The enzyme activity in the liver of rats given 80% protein diet or glucagon injection during starvation increased to 2- to 2.4-fold that in the liver of rats ma...

journal_title：Journal of biochemistry

authors： Horio Y,Tanaka T,Taketoshi M,Uno T,Wada H

更新日期：1988-05-01 00:00:00

abstract：:Genetic engineering strategies were applied to synthesize silk-like materials, [(GVPGV)(2)GG(GAGAGS)(3)AS](n). The primary structure of these materials represents the repetitive crystalline region of Bombyx mori silk fibroins incorporated with an elastic motif selected from animal elastin. The oligonucleotides were de...

journal_title：Journal of biochemistry

authors： Yao J,Asakura T

更新日期：2003-01-01 00:00:00

abstract：:Long non-coding RNA is an endogenous non-coding RNA that has currently been proved to be an important player in cancer cell biology. In the present study, we investigated the biological role of PHACTR2-AS1 in tongue squamous cell carcinoma (TSCC). PHACTR2-AS1 was preferentially localized in the cytoplasm, and was nota...

journal_title：Journal of biochemistry

authors： Yuan F,Miao Z,Chen W,Wu F,Wei C,Yong J,Xiao C

更新日期：2020-12-26 00:00:00

abstract：:Elafin was shown to be a new type of proteinase inhibitor which has an anchoring sequence. Human elafin, a potent inhibitor specific for elastase and proteinase 3, has a unique repeating sequence in its prosegment that is rich in Gln and Lys residues. The prosegment, termed "cementoin," exhibits high homology with the...

journal_title：Journal of biochemistry

authors： Nara K,Ito S,Ito T,Suzuki Y,Ghoneim MA,Tachibana S,Hirose S

更新日期：1994-03-01 00:00:00

abstract：:His397 was replaced with alanine by site-directed mutagenesis of the cloned PRC1 gene in order to confirm the role of this residue in the proton-relay system of carboxypeptidase Y (CPY). The expressed and purified H397A showed a CD spectrum almost identical to that of the wild type enzyme, but its heat stability and c...

journal_title：Journal of biochemistry

authors： Jung G,Ueno H,Hayashi R

更新日期：1998-08-01 00:00:00

abstract：:The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release from microsomes of corn coleoptiles was investigated. Addition of micromolar concentrations of IP3 to Ca2+ loaded microsomes resulted in rapid release of 20-30% of sequestered Ca2+. Maximal and half maximal Ca2+ release occurred at 20 and 8 microM of IP3...

journal_title：Journal of biochemistry

authors： Reddy AS,Poovaiah BW

更新日期：1987-03-01 00:00:00

abstract：:Previously, several workers reported that at very low ionic strength and in the presence of ATP, the extent of binding of S-1 with the F-actin-tropomyosin-troponin complex or regulated actin (FA-TM-TN) is unaffected by removal of Ca2+. However, in this study we found that during the ATPase reaction at physiological io...

journal_title：Journal of biochemistry

authors： Inoue A,Tonomura Y

更新日期：1982-04-01 00:00:00

abstract：:The enzymes phosphoglucomutase (PGM) and phosphomannomutase (PMM) play an important role in the synthesis of extracellular polysaccharide. By colony hybridization of the fosmid library of Sphingomonas chungbukensis DJ77, an open reading frame (ORF-1) of 1,626 nucleotides, whose predicted product is highly homologous w...

journal_title：Journal of biochemistry

authors： Yoon SS,Park SH,Kim YC,Shin M,Chong CK,Choi JD

更新日期：2008-10-01 00:00:00

abstract：:Xanthine dehydrogenase has been purified to a homogeneous state from cell-free extracts of a strain of Streptomyces. The enzyme has a molecular weight of 125,000 and consists of two subunits with a molecular weight of 67,000. The isoelectric point is at pH 4.4. The enzyme exhibits absorption maxima at 273, 355, and 45...

journal_title：Journal of biochemistry

authors： Ohe T,Watanabe Y

更新日期：1979-07-01 00:00:00

abstract：:Partition of merocyanine dyes, which have a negative charge, onto photosynthetic membranes of chloroplasts and bacteria was analyzed by measuring the fluorescence intensity change, absorbance change, and amount of dye in the supernatant after centrifugation. The partition depended on the surface potential, which is a ...

journal_title：Journal of biochemistry

authors： Masamoto K,Matsuura K,Itoh S,Nishimura M

更新日期：1981-02-01 00:00:00

abstract：:HSP47 is a stress protein (heat shock protein) which resides in the endoplasmic reticulum, and is postulated to function as a collagen-specific molecular chaperone. To elucidate the role of HSP47 in procollagen biosynthesis, we have established human embryonic kidney 293 cell lines, which were stably transfected with ...

journal_title：Journal of biochemistry

authors： Hosokawa N,Hohenadl C,Satoh M,Kühn K,Nagata K

更新日期：1998-09-01 00:00:00

abstract：:The light protein components of silk fibroin were analyzed by Western blotting using polyclonal antibodies against two synthetic peptides that are segment of the light (L-) chain [Yamaguchi et al. (1989) J. Mol. Biol. 210, 127-139] and P25 [Chevillard et al. (1986) Sericologia 26, 435-449], respectively, and against t...

journal_title：Journal of biochemistry

authors： Tanaka K,Mori K,Mizuno S

更新日期：1993-07-01 00:00:00

abstract：:Three monoclonal antibodies (MAbs1A2, 3C5, and 4C2) for human aldolase A [EC 4.1.2.13] were established. MAbs1A2, 3C5, and 4C2 were shown to belong to subclasses IgM, IgG1, and IgG2a, respectively. None of the MAbs inhibits aldolase A activity. Their epitopes were mapped in detail on the molecule by examining the reac...

journal_title：Journal of biochemistry

authors： Kitajima Y,Matsuhashi S,Nishida H,Takasaki Y,Takahashi I,Hisatsugu T,Hori K

更新日期：1991-04-01 00:00:00

abstract：:Using an immunoblotting technique, we have studied the processing of plasma proteins in subcellular fractions of rat liver including rough and smooth microsomes and the Golgi subfractions. Each subcellular fraction was directly subjected to SDS-polyacrylamide gel electrophoresis and analyzed by immunoblotting with ant...

journal_title：Journal of biochemistry

authors： Oda K,Miki K,Hirose S,Takami N,Misumi Y,Ikehara Y

更新日期：1986-12-01 00:00:00

abstract：:The sequential in situ digestion of proteins immobilized on polyvinylidene difluoride (PVDF) has been systematically designed and optimized. The method consists of immobilization of the proteins on PVDF, S-carboxymethylation, and then successive in situ digestions using specific proteases. In order to obtain high yiel...

journal_title：Journal of biochemistry

authors： Iwamatsu A,Yoshida-Kubomura N

更新日期：1996-07-01 00:00:00

abstract：:The nucleotide sequence of the aspartate aminotransferase [EC 2.6.1.1] structural gene, aspC, of Escherichia coli K-12 was determined. The coding region of the aspC gene contained 1,188 nucleotide residues and encoded 396 amino acid residues. The amino acid sequence deduced from the nucleotide sequence agreed perfectl...

journal_title：Journal of biochemistry

authors： Kuramitsu S,Okuno S,Ogawa T,Ogawa H,Kagamiyama H

更新日期：1985-04-01 00:00:00

abstract：:A new assay method for hydrogenase [EC 1.12.2.1] based on the enzymic electrode reaction of H2-H+ equilibrium has been established. The method is based on the experimental fact that the short-circuit current of the electric cell composed of an electrode with hydrogenase and methylviologen as the mediator of H2-H+ equi...

journal_title：Journal of biochemistry

authors： Yagi T,Goto M,Nakano K,Kimura K,Inokuchi H

更新日期：1975-09-01 00:00:00

abstract：:The characteristics of the androgen receptor in the cytoplasmic fraction of Shionogi carcinoma 115 were studied in vitro by means of charcoal adsorption assay, sucrose density gradient centrifugation, and polyacrylamide gel electrophoresis. The equilibrium dissociation constant for [3H]dihydrotestosterone (Kd = (2-5) ...

journal_title：Journal of biochemistry

authors： Nohno T

更新日期：1981-02-01 00:00:00

abstract：:The genomic organization of the gene encoding the human adipocyte-derived leucine aminopeptidase (A-LAP) has been determined. The gene is composed of 20 exons and 19 introns and spans approximately 47 kilobases of chromosome 5q15. The gluzincin aminopeptidase motif, the HEXXH(X)(18)E zinc-binding motif essential for e...

journal_title：Journal of biochemistry

authors： Hattori A,Matsumoto K,Mizutani S,Tsujimoto M

更新日期：2001-08-01 00:00:00