Systematic peptide fragmentation of polyvinylidene difluoride(PVDF)-immobilized proteins prior to microsequencing.

abstract：:The role of HSDJ, a human homolog of bacterial DnaJ and yeast YDJ1p/MAS5, in mitochondrial protein import was examined. Recombinant HSDJ was purified and an antibody was prepared. HSDJ mRNA was heat-induced in cultured cells. In pulse-labeling and chase experiments using COS-7 cells, the endogenous HSDJ homolog was pr...

journal_title：Journal of biochemistry

authors： Kanazawa M,Terada K,Kato S,Mori M

更新日期：1997-05-01 00:00:00

abstract：:Lactosylceramide and paragloboside were synthesized from their precursor glycolipids and UDP-galactose by lactose synthase A protein [UDP-Gal : GlcNAc beta-4-galactosyltransferase, EC 2.4.1.22] purified to homogeneity from human plasma. The partially purified human liver enzyme and an extract from human lymphoblastoid...

journal_title：Journal of biochemistry

authors： Yamato K,Yoshida A

更新日期：1982-10-01 00:00:00

abstract：:Pure recA protein was easily obtained from Escherichia coli harboring plasmid pTM-2 which carried the recA gene by two chromatographic steps on phosphocellulose and DEAE-cellulose. RecA protein was stable in the pH range of 6 to 9 at 25 degrees C. RecA protein was found to aggregate highly under these conditions. Lowe...

journal_title：Journal of biochemistry

authors： Kuramitsu S,Hamaguchi K,Ogawa T,Ogawa H

更新日期：1981-10-01 00:00:00

abstract：:Site-directed mutagenesis studies of recombinant Trimeresurus flavoviridis venom gland phospholipase A2 (PLA2) showed that the Asn residue at position 67 takes part in recognition of the substrate 2-arachidonoyl sn-glycero-3-phosphocholine in both monomeric and micellar states. The amount of arachidonate released from...

journal_title：Journal of biochemistry

authors： Ogawa T,Shimohigashi Y,Ohno M

更新日期：1997-11-01 00:00:00

abstract：:Sliding machines composed of F-actin and myosin or microtubules and kinesin or dynein convert the free energy of ATP hydrolysis into sliding movements and mechanical work. Development of optical microscopy with micromanipulation techniques has made possible direct observation of single events of sliding exhibited by s...

journal_title：Journal of biochemistry

authors： Oosawa F

更新日期：1995-11-01 00:00:00

abstract：:alpha-Actinin purified from chicken gizzard smooth muscle was characterized in comparison with alpha-actinins from chicken striated muscles, or fast-skeletal muscle, slow-skeletal muscle, and cardiac muscle. The gizzard alpha-actinin molecule consisted of two apparently identical subunits with a molecular weight of 10...

journal_title：Journal of biochemistry

authors： Endo T,Masaki T

更新日期：1982-11-01 00:00:00

abstract：:The amino acid sequence of glutathione S-transferase a from guinea pig liver was determined. Glutathione S-transferase a was composed of two identical subunits, each comprising 218 amino acid residues. The amino acid sequence of glutathione S-transferase a exhibited 73% homology with that of human glutathione S-transf...

journal_title：Journal of biochemistry

authors： Kamei-Hayashi K,Oshino R,Hara S

更新日期：1993-12-01 00:00:00

abstract：:A beta-galactosidase was extracted from the internal organs of a sea squirt, Styela plicata, and purified 959-fold, with an 18% yield, by successive gel chromatography, anion-exchange chromatography, chromatofocusing, and affinity chromatography on a Con A-Sepharose column. The purified enzyme was fairly homogeneous, ...

journal_title：Journal of biochemistry

authors： Shigeta S,Ono K,Oka S

更新日期：1991-07-01 00:00:00

abstract：:E. coli bearing hybrid plasmid pKOl (Oeda et al. (1981) Mol. Gen. Genet. 184, 191-199) expressed a large amount of lysophospholipase L2 activity. When a mutant which was defective in lysophospholipase L2 activity was transformed with plasmid pKOl, it overproduced lysophospholipase L2 activity. The gene responsible for...

journal_title：Journal of biochemistry

authors： Homma H,Kobayashi T,Ito Y,Kudo I,Inoue K,Ikeda H,Sekiguchi M,Nojima S

更新日期：1983-12-01 00:00:00

abstract：:The time course of binding of N-ethylmaleimide (NEM) to the SR was measured at pH 7.5 in the presence or absence of ATP or ADP. The following results were obtained. 1. Both in the presence and absence of nucleotide, the ATPase [EC 3.6.1.3] activity decreased linearly with increase in the amount of NEM bound to the fra...

journal_title：Journal of biochemistry

authors： Yoshida H,Tonomura Y

更新日期：1976-03-01 00:00:00

abstract：:Human, bovine, and mouse C1q, a subcomponent of the first complement component, were purified, and both globular (GF) and collagen-like fragments (CLF) were isolated from human and bovine C1q. Antisera were produced in rabbits with these C1q or fragments, and F(ab')2 of immunoglobulin G (IgG) was purified from the ant...

journal_title：Journal of biochemistry

authors： Yonemasu K,Sasaki T,Shimizu A

更新日期：1983-04-01 00:00:00

abstract：:The prothrombin-converting activity of Factor Xa was enhanced by thrombin-stimulated Factor V-deficient platelets and supplementary extraneous Factor Va, and also by thrombin-stimulated normal human platelets. Both extraneous Factor Va and intra-platelet Factor Va were equally inactivated by a gamma-carboxyglutamic ac...

journal_title：Journal of biochemistry

authors： Suzuki K,Nishioka J,Matsuda M,Murayama H,Hashimoto S

更新日期：1984-08-01 00:00:00

abstract：:The conformational changes of ribonucleic acid induced by heat treatment were studied by measurements of fluorescence polarization, circular dichroism, and ultraviolet absorption. The fluorescence polarization of proflavine covalently bound to the 3'-end of 16S ribosomal RNA of Escherichia coli and that of ethenoadeno...

journal_title：Journal of biochemistry

authors： Kumagai JI,Ohta T,Imahori K

更新日期：1976-03-01 00:00:00

abstract：:Corynebacterium diphtheriae, a pathogenic Gram-positive bacterium, contains sialic acids on its cell surface, but no genes related to sialic acid decoration or metabolism have been reported in C. diphtheriae. In the present study, we have identified a putative sialidase gene, nanH, from C. diphtheriae KCTC3075 and cha...

journal_title：Journal of biochemistry

authors： Kim S,Oh DB,Kwon O,Kang HA

更新日期：2010-04-01 00:00:00

abstract：:The scaffolding protein Salvador (Sav) plays a key role in the Hippo (Hpo) signalling pathway, which controls tissue growth by inhibiting cell proliferation and promoting apoptosis. Dysregulation of the Hippo pathway contributes to cancer development. Since the identification of the first Sav gene in 2002, very little...

journal_title：Journal of biochemistry

authors： Song J,Hong HR,Yamashita E,Park IY,Lee SJ

更新日期：2015-09-01 00:00:00

abstract：:Aggregate formation of recombinant human erythropoietin (r-EPO) on heat-treatment was followed by gel permeation chromatography combined with a low-angle laser light scattering technique under various conditions with respect to pH and salt concentration in order to provide basic knowledge about the change strictly req...

journal_title：Journal of biochemistry

authors： Endo Y,Nagai H,Watanabe Y,Ochi K,Takagi T

更新日期：1992-11-01 00:00:00

abstract：:The contribution of the P1' residue at the first reactive site of peanut protease inhibitor B-III to the inhibition was analyzed by replacement of the P1' Arg(11) with other amino acids (Arg, Ser, Ala, Leu, Phe, Asp) after selective modification of the second reactive site. The Arg derivative had the same trypsin inhi...

journal_title：Journal of biochemistry

authors： Kurokawa T,Hara S,Norioka S,Teshima T,Ikenaka T

更新日期：1987-03-01 00:00:00

abstract：:When an aqueous solution of a spin-labeled "two tail" gangliosidoid was incubated with liposomes or sheep erythrocytes, the broad single resonance line in the ESR spectrum disappeared and a signal showing an anisotropic motion appeared, indicating that the spin-labeled "two tail" gangliosidoid in the micellar state wa...

journal_title：Journal of biochemistry

authors： Kanda S,Inoue K,Nojima S,Utsumi H,Wiegandt H

更新日期：1982-06-01 00:00:00

abstract：:Three forms (termed I, II, and III) of ribonuclease H (RNase H) [EC 3.1.4.34] activity are present in rat liver cytosol. These enzymes degrade RNA specifically in RNA-DNA hybrid structures. They were eluted at 0 M, 0.25 M, and 0.5 M KCl in phosphocellulose chromatography, and were further purified by using blue Sephar...

journal_title：Journal of biochemistry

authors： Sawai Y,Yanokura M,Tsukada K

更新日期：1979-09-01 00:00:00

abstract：:A fluorescent photoreactive ATP derivative, 2'(3')-O-(4-benzoylbenzoyl)-1,N(6)-etheno-ATP (Bz(2)-epsilonATP), was synthesized and reacted with the rice kinesin K16 motor domain (K16MD). In the presence of ADP or ATP, UV irradiation of the K16MD solution containing Bz(2)-epsilonATP resulted in a new 100 kDa band, which...

journal_title：Journal of biochemistry

authors： Umeki N,Mitsui T,Koike Y,Maruta S

更新日期：2006-05-01 00:00:00

abstract：:Expression of structural proteins of human immunodeficiency virus type 1 requires the direct interaction of the viral Rev trans-activator with its cis-acting RNA target sequence, the Rev response element (RRE). The originally defined 234-residue RRE, however, failed to show the full HIV-1 regulator of virion expressio...

journal_title：Journal of biochemistry

authors： Kimura T,Ohyama A

更新日期：1994-05-01 00:00:00

abstract：:Disulfide bond interchange has been pointed out as a considerable problem in preparing recombinant proteins from Escherichia coli cells. This has been reported in the system of reducing denaturation followed by a refolding process, where incorrectly folded molecules are sometimes produced. As the possibility of disulf...

journal_title：Journal of biochemistry

authors： Kimura S,Utsumi J,Yamazaki S,Shimizu H

更新日期：1988-07-01 00:00:00

abstract：:Methyl-coenzyme M reductase isoenzyme I from the methanogenic Archaeon, Methanobacterium thermoautotrophicum (strain Marburg), was crystallized by vapor diffusion methods. Crystal form M obtained with 2-methyl-2,4-pentanediol as the precipitant displayed space group P2(1), with unit cell parameters of a=83.2 A, b=117....

journal_title：Journal of biochemistry

authors： Shima S,Goubeaud M,Vinzenz D,Thauer RK,Ermler U

更新日期：1997-05-01 00:00:00

abstract：:Poly(8-oxyinosinic acid) (poly O8I), was synthesized by polymerizing 8-oxyinosine diphosphate using the enzyme polynucleotide phosphorylase (PNPase). The polymer formed a 1 : 1 hybrid with polycytidylic acid (poly C). The hybrid was found to induce the production of interferon in the brain of white albino mice and pro...

journal_title：Journal of biochemistry

authors： Folayan JO

更新日期：1982-09-01 00:00:00

abstract：:The patterns of myosin isozymes in embryonic and adult chicken pectoralis muscle were examined by electrophoresis in a non-denaturing gel system (pyrophosphate acrylamide gel electrophoresis), and both light chains and heavy chains of embryonic and adult myosin isozymes were compared. In pyrophosphate acrylamide gel e...

journal_title：Journal of biochemistry

authors： Takano-Ohmuro H,Obinata T,Masaki T,Mikawa T

更新日期：1982-04-01 00:00:00

abstract：:Lipoamide dehydrogenase (LADase) was purified to homogeneity from rat liver mitochondria, and the intracellular distribution and biosynthesis of the LADase were investigated with antibody prepared against the purified enzyme. 1) LADase activity was mostly found in mitochondria; the activity in cytosol was about one-te...

journal_title：Journal of biochemistry

authors： Matuda S,Saheki T

更新日期：1982-02-01 00:00:00

abstract：:The most abundant glycoprotein in the coelomic fluid of sea urchin Anthocidaris crassispina was purified and its subunit structure, molecular form in the native state, amino acid composition, and electron micrographic image were studied. The results showed that the protein in its native state was basically a tetramer ...

journal_title：Journal of biochemistry

authors： Giga Y,Ikai A

更新日期：1985-07-01 00:00:00

abstract：:The effect of inositol 1,4,5-trisphosphate (IP3) on Ca2+ release from microsomes of corn coleoptiles was investigated. Addition of micromolar concentrations of IP3 to Ca2+ loaded microsomes resulted in rapid release of 20-30% of sequestered Ca2+. Maximal and half maximal Ca2+ release occurred at 20 and 8 microM of IP3...

journal_title：Journal of biochemistry

authors： Reddy AS,Poovaiah BW

更新日期：1987-03-01 00:00:00

abstract：:Ten Drosophila thorax proteins (six myosin light chains and four proteins called a, b, c, d) were found to have high affinities with Ca2+. This was proved after subjecting the total Drosophila thorax proteins to two-dimensional (2D) transblot, followed by 45Ca2+ autoradiography. Three proteins (a, c, d) showed Ca2+ de...

journal_title：Journal of biochemistry

authors： Tanaka Y,Maruyama K,Mikawa T,Hotta Y

更新日期：1988-10-01 00:00:00

abstract：:Ectoenzyme release from rat liver and kidney by phosphatidylinositol (PI)-specific phospholipase C of Bacillus thuringiensis was studied. Alkaline phosphatase and 5'-nucleotidase were released from rat kidney slices to extents of up to 60% and 30%, respectively. Release of alkaline phosphatase was observed at lower am...

journal_title：Journal of biochemistry

authors： Taguchi R,Asahi Y,Ikezawa H

更新日期：1985-03-01 00:00:00