Identification and cloning of the gene coding for lysophospholipase L2 of E. coli K-12.

Abstract:

:E. coli bearing hybrid plasmid pKOl (Oeda et al. (1981) Mol. Gen. Genet. 184, 191-199) expressed a large amount of lysophospholipase L2 activity. When a mutant which was defective in lysophospholipase L2 activity was transformed with plasmid pKOl, it overproduced lysophospholipase L2 activity. The gene responsible for the lysophospholipase L2 activity was designated as pld B. On the same hybrid plasmid another gene (pld A) coding for detergent-resistant phospholipase A (DR-phospholipase A) was also identified. These facts together with the results of a Pl transduction experiment revealed that the pld B gene must be between the pld A and met E genes on the E. coli chromosome.

journal_name

J Biochem

journal_title

Journal of biochemistry

authors

Homma H,Kobayashi T,Ito Y,Kudo I,Inoue K,Ikeda H,Sekiguchi M,Nojima S

doi

10.1093/oxfordjournals.jbchem.a134566

subject

Has Abstract

pub_date

1983-12-01 00:00:00

pages

2079-81

issue

6

eissn

0021-924X

issn

1756-2651

journal_volume

94

pub_type

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