Production of monoclonal antibodies directed to Hanganutziu-Deicher active gangliosides, N-glycolylneuraminic acid-containing gangliosides.


:We have established three kinds of monoclonal antibodies against gangliosides containing N-glycolylneuraminic acid (NeuGc) by immunization of BALB/c mice with the purified gangliosides inserted into liposomes comprising Salmonella minnesota R595 lipopolysaccharides, and fusion of spleen cells with a mouse myeloma cell line. One monoclonal antibody, SHS-1, which was generated by immunizing mice with purified i-active ganglioside(NeuGc), reacted specifically with the i-active ganglioside(NeuGc) used as an immunogen. Structurally related gangliosides, such as GM3(NeuGc), sialosylparagloboside (SPG) (NeuGc), or I-active ganglioside(NeuGc), corresponding gangliosides [GM3 containing N-acetylneuraminic acid (NeuAc), SPG(NeuAc), i-active ganglioside(NeuAc), and I-active ganglioside(NeuAc)], other gangliosides, or neutral glycosphingolipid (GSL) were not recognized by the monoclonal antibody. These findings indicate that the SHS-1 monoclonal antibody may be specific for NeuGc-containing i-active ganglioside. On the other hand, the other two monoclonal antibodies, MSG-1 and SPS-20, which were generated by immunizing mice with purified ganglioside GM3(NeuGc) and SPG(NeuGc), respectively, showed cross-reactivity to structurally related gangliosides. The MSG-1 monoclonal antibody exhibited reactivity to ganglioside GM3(NeuAc). The SPS-20 monoclonal antibody also cross-reacted with SPG(NeuAc), i-active ganglioside(NeuGc), and i-active ganglioside(NeuAc). Neither MSG-1 nor SPS-20 reacted with corresponding gangliosides, other gangliosides, or neutral GSLs tested. Using the SHS-1 antibody specific for i-active ganglioside(NeuGc), we studied the expression of NeuGc-containing antigen in human colon cancer tissue. An NeuGc-containing glycoconjugate was detected in the colon cancer tissue.


J Biochem


Journal of biochemistry


Watarai S,Kushi Y,Shigeto R,Misawa N,Eishi Y,Handa S,Yasuda T




Has Abstract


1995-05-01 00:00:00












  • Preparation and chemical characterization of the three chains of the major hemoglobin of the sea snake, Pelamis platurus.

    abstract::One of the 2 main hemoglobins of the sea snake Pelamis platurus, (the Yellow-bellied sea snake) comprising about 70% of the total hemoglobin, was separated by DEAE-Sephadex column chromatography. From results of gel filtration and iron content determination, both intact sea snake hemoglobin, and the isolated major hem...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Liu CS

    更新日期:1975-07-01 00:00:00

  • A rapid filtration assay for cAMP.

    abstract::The receptor-binding assay for cAMP was improved by using polyethylenimine-treated glass filters. A polyethylenimine-treated glass filter has high protein binding capacity. This high capacity allows an increase in the amount of protein per assay tube and the use of a crude preparation, such as a beef heart extract, as...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Takeda T,Kuno T,Shuntoh H,Tanaka C

    更新日期:1989-02-01 00:00:00

  • A continuous cell-free protein synthesis system for coupled transcription-translation.

    abstract::A continuous cell-free protein synthesis system [Spirin et al. (1988) Science 242, 1162-1164] was modified so as to be suitable for coupled transcription-translation, a process useful for obtaining products of cloned genes or cDNAs. A reaction chamber equipped with an ultrafiltration membrane was newly designed and an...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Kigawa T,Yokoyama S

    更新日期:1991-08-01 00:00:00

  • Amino acid sequence of an active fragment of potato proteinase inhibitor IIa.

    abstract::The complete amino acid sequence of an active fragment of potato proteinase inhibitor IIa has been established by the Edman degradation procedure and the carboxypeptidase technique. Sequence analyses were carried out on the reduced and carboxymethylated active fragment and its tryptic peptides. To aid in the alignment...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Iwasaki T,Kiyohara T,Yoshikawa M

    更新日期:1976-02-01 00:00:00

  • Oxygen exchange reaction during ATP hydrolysis by glycerinated muscle fibers, myofibrils, and synthetic actomyosin filaments.

    abstract::The oxygen exchange during ATP hydrolysis by glycerinated muscle fibers, myofibrils, and synthetic actomyosin filaments was studied from the distribution of the [18O]Pi species produced by the hydrolysis of [gamma-18O]ATP. The products were mixtures of two species, one with a low extent of oxygen exchange and the othe...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yasui M,Ohe M,Kajita A,Arata T,Inoue A

    更新日期:1989-04-01 00:00:00

  • Primary structure and autoproteolysis of brevilysin H6 from the venom of Gloydius halys brevicaudus.

    abstract::The complete amino acid sequence of brevilysin H6 (H6), a zinc-protease isolated from Gloydius halys brevicaudus venom, was determined by a manual Edman degradation method. H6 has an amino-terminal pyroglutamic acid and consists of a total of 419 residues. An N-linked sugar chain is attached at Asn-181. The molecule i...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujimura S,Oshikawa K,Terada S,Kimoto E

    更新日期:2000-08-01 00:00:00

  • Characterization of recombinant YakC of Schizosaccharomyces pombe showing YakC defines a new family of aldo-keto reductases.

    abstract::The yakC gene in Schizosaccharomyces pombe, which encodes yakC protein (YakC), a potential member of an aldo-keto reductase (AKR) family, was cloned and expressed in Escherichia coli cells. The recombinant YakC purified to homogeneity catalyzed the reduction of 2-nitrobenzaldehyde (k(cat), 44.1 s(-1), K(m), 0.185 +/- ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Morita T,Huruta T,Ashiuchi M,Yagi T

    更新日期:2002-10-01 00:00:00

  • Subsite preferences of pepstatin-insensitive carboxyl proteinases from bacteria.

    abstract::Pseudomonas sp. 101 carboxyl proteinase (PCP) and Xanthomonas sp. T-22 carboxyl proteinase (XCP), the first and second unique carboxyl proteinases from prokaryotes to be isolated and characterized, are not inhibited by the classical carboxyl proteinase inhibitor pepstatin. In this study, we elucidated their subsite pr...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Narutaki S,Dunn BM,Oda K

    更新日期:1999-01-01 00:00:00

  • Biosynthesis of rat kidney catalase. Double-labeling by [14C]leucine and delta-amino[3H]levulinic acid.

    abstract::Kidney catalase [EC] was doubly labeled by injecting a mixture of [14C]-leucine and delta-amino[3H]levulinic acid (ALA) into normal and allyliso-(2-isopropyl-4-pentenoyl)urea (Sedormid)-treated rats. Shortly after the injection of a tracer dose or the precursors, catalase in microsomes showed the highest spec...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Higashi T,Kawamata F,Dogen M

    更新日期:1976-08-01 00:00:00

  • Molecular architectures and functions of radical enzymes and their (re)activating proteins.

    abstract::Certain proteins utilize the high reactivity of radicals for catalysing chemically challenging reactions. These proteins contain or form a radical and therefore named 'radical enzymes'. Radicals are introduced by enzymes themselves or by (re)activating proteins called (re)activases. The X-ray structures of radical enz...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Shibata N,Toraya T

    更新日期:2015-10-01 00:00:00

  • A screening system for artificial small RNAs that inhibit the growth of Escherichia coli.

    abstract::We have developed a screening system for artificial small RNAs (sRNAs) that inhibit the growth of Escherichia coli. In this system, we used a plasmid library to express artificial sRNAs (approximately 200 bases long) containing 60 bases of random nucleotide sequence. The induced expression of the known rydB sRNA in th...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Komasa M,Fujishima K,Hiraoka K,Shinhara A,Lee BS,Tomita M,Kanai A

    更新日期:2011-09-01 00:00:00

  • Interaction of the Escherichia coli lipoprotein NlpI with periplasmic Prc (Tsp) protease.

    abstract::Escherichia coli spr (suppressor of prc) mutants and nlpI mutants show thermosensitive growth. The thermosensitivity of the spr mutants was suppressed by the nlpI mutations. Expression of the fusion genes encoding hexa-histidine-tagged NlpI (NlpI-His) and purification of the tagged NlpI showed that NlpI-His bound with...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tadokoro A,Hayashi H,Kishimoto T,Makino Y,Fujisaki S,Nishimura Y

    更新日期:2004-02-01 00:00:00

  • Tetrahymena calcium-binding protein is indeed a calmodulin.

    abstract::We previously isolated a Ca2+-binding protein from a ciliate, Tetrahymena, and designated it as TCBP (Tetrahymena Ca2+-binding protein). The present paper reports that TCBP, which has two high affinity Ca2+-binding sites (Kd=4.6 X 10(-6) M), could activate porcine brain cyclic nucleotide phosphodiesterase at a concent...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Suzuki Y,Nagao S,Abe K,Hirabayashi T,Watanabe Y

    更新日期:1981-01-01 00:00:00

  • A study on the identities of the three species of chromatin-associated proteinases in a mutant of Saccharomyces cerevisiae which lacks four major vacuolar proteinases.

    abstract::Using mutant strain ABYS1 of Saccharomyces cerevisiae lacking four main vacuolar proteinases, proteinase A, proteinase B, carboxypeptidase Y, and carboxypeptidase S, we examined the identities of chromatin-associated proteinases, ruling out possible contamination of the chromatin fraction by them. The chromatin of str...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Motizuki M,Kohno H,Tsurugi K

    更新日期:1988-08-01 00:00:00

  • Phosphorylation of CaBP1 and CaBP2 by protein kinase CK2.

    abstract::Several proteins in the mammalian endoplasmic reticulum are substrates for protein kinases. Many unidentified phosphoproteins from this compartment are described in the literature, and this prompted us to try to identify at least the more dominant ones. When solubilized bovine and murine microsomes were phosphorylated...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Janson IM,Ek B,Ek P

    更新日期:1997-01-01 00:00:00

  • Preparation of monoclonal antibodies against N-(gamma-maleimidobutyryloxy)succinimide (GMBS)-conjugated acetylspermine, and development of an enzyme-linked immunosorbent assay (ELISA) for N1,N12-diacetylspermine.

    abstract::We have developed three mouse monoclonal antibodies (mAb) of types IgG1 and IgG2b, i.e. anti-acetylspermine (Ac-Spm)-1 and 2 (ACSPM-1 and 2), and anti-acetylspermine (Ac-Spm)-3 (ACSPM-3), respectively, against Ac-Spm conjugated to bovine serum albumin via a heterobifunctional cross-linker, N-(gamma-maleimidobutyryloxy...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Fujiwara K,Kaminishi Y,Kitagawa T,Tsuru D,Yabuuchi M,Kanetake H,Nomata K

    更新日期:1998-07-01 00:00:00

  • Differential and additive effects of the three conserved isoleucine residues in the promoter -10 binding region on Bacillus subtilis sigma(A) structure and function.

    abstract::The promoter -10 binding region of the Bacillus subtilis sigma(A) factor forms an amphiphilic alpha-helix with three conserved isoleucines located at four-residue intervals. To investigate the structural and functional roles of the three isoleucine residues, we constructed a series of sigA mutants with single and doub...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Liao CT,Wang WH,Yang HS,Chen JP,Chang BY

    更新日期:1999-09-01 00:00:00

  • Duality of alpha-subunit of canine kidney sodium- and potassium-activated adenosinetriphophatase in electrophoresis.

    abstract::Sodium- and potassium-activated adenosinetriphosphatase (Na+, K+-ATPase) purified from dog kidney outer medulla was examined by polyacrylamide gel electrophoresis and by photoaffinity labeling with N-(ouabain)-N'-(2-nitro-4-azidophenyl)-ethylenediamine (NAP-ouabain). The large subunit band (alpha-band) split into two ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ohta T,Kawamura M,Hasegawa T,Ishikura H,Nagano K

    更新日期:1982-01-01 00:00:00

  • Existence of stoichiometric amounts of an intrinsic ATPase inhibitor and two stabilizing factors with mitochondrial ATP synthase in yeast.

    abstract::Two proteinaceous factors, 15K and 9K proteins, which acted together to stabilize the inactivated yeast F1F0-ATPase-inhibitor complex [Hashimoto, T., et al. (1984) J. Biochem. 95, 131-136] were hardly distinguishable from the sigma and epsilon subunits, respectively, of yeast F1-ATPase by sodium dodecyl sulfate (SDS) ...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Okada Y,Hashimoto T,Yoshida Y,Tagawa K

    更新日期:1986-01-01 00:00:00

  • Galectin-1 from bovine spleen: biochemical characterization, carbohydrate specificity and tissue-specific isoform profiles.

    abstract::Selected biochemical properties, including the charge heterodispersity profile and carbohydrate specificity, of bovine galectin-1 were determined in detail. The lectin was purified through an improved purification protocol that yielded 35-40 mg/kg of wet tissue with a specific activity of 1.7-2 x 10(4) The ga...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Ahmed H,Fink NE,Pohl J,Vasta GR

    更新日期:1996-11-01 00:00:00

  • Mechanism-based inactivation of coenzyme B12-dependent diol dehydratase by 3-unsaturated 1,2-diols and thioglycerol.

    abstract::The reactions of diol dehydratase with 3-unsaturated 1,2-diols and thioglycerol were investigated. Holodiol dehydratase underwent rapid and irreversible inactivation by either 3-butene-1,2-diol, 3-butyne-1,2-diol or thioglycerol without catalytic turnovers. In the inactivation, the Co-C bond of adenosylcobalamin under...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Toraya T,Tamura N,Watanabe T,Yamanishi M,Hieda N,Mori K

    更新日期:2008-10-01 00:00:00

  • Exploring the nucleotide-binding site in proteins by affinity labeling and site-directed mutagenesis.

    abstract::Affinity labeling with nucleoside polyphosphopyridoxals, especially those with 3 or 4 phosphate groups, was effective for identifying the lysyl residue(s) located at or near the binding site for ATP, GTP, UDP-Glc, or ADP-Glc in various proteins. Furthermore, kinetic analysis of the mutant enzymes, in which the labeled...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Fukui T

    更新日期:1995-06-01 00:00:00

  • Cloning of dihydrofolate reductase gene of Escherichia coli K12.

    abstract::Resistant strains for trimethoprim, a potent inhibitor of dihydrofolate reductase, were obtained by transforming the ligated products of Escherichia coli K12 DNA and plasmid pBR322 BamH I fragments. The strains carry a 13.6 kbp plasmid, pTP1, which contains the trimethoprim- and ampicillin-resistance determinant genes...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Iwakura M,Shimura Y,Tsuda K

    更新日期:1982-04-01 00:00:00

  • Static and kinetic studies on rabbit skeletal muscle troponin.

    abstract::Fluorescence titration curves of 2-[4'-iodoacetamido)anilino)naphthalene-6-sulfonic acid-labeled troponin (IAANS-labeled Tn) and troponin-1-anilinonaphthalene-8-sulfonic acid (Tn-ANS) complex indicated that the fluorescent moiety, IAANS or ANS, detects conformational change of troponin I (TnI) or Tn due to the Ca2+ bi...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Nishio T,Iio T

    更新日期:1983-09-01 00:00:00

  • Effect of urea on protein separation by ion-exchange chromatography.

    abstract::Ion-exchange chromatography (IEC) is the most frequently used chromatographic technique for the separation of proteins and peptides. In this article, the effects of urea on IEC separation of kiwifruit actinidin, egg white and urinary proteins were examined. The purity and relative amount of each protein in different c...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Khademi F,Mostafaie A

    更新日期:2010-05-01 00:00:00

  • Close association of B2 bradykinin receptors with P2Y2 ATP receptors.

    abstract::Two G-protein-coupled receptors (GPCRs) that couple with Gαq/11, B2 bradykinin (BK) receptor (B2R) and ATP/UTP receptor P2Y2 (P2Y2R), are ubiquitously expressed and responsible for vascular tone, inflammation, and pain. We analysed the cellular signalling of P2Y2Rs in cells that express B2Rs. B2R desensitization induc...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Yashima S,Shimazaki A,Mitoma J,Nakagawa T,Abe M,Yamada H,Higashi H

    更新日期:2015-08-01 00:00:00

  • Secretion of an active nonglycosylated form of the repressible acid phosphatase of Saccharomyces cerevisiae in the presence of tunicamycin at low temperatures.

    abstract::The role of mannan chains in the formation and secretion of active acid phosphatase of yeast (Saccharomyces cerevisiae), a repressible cell surface mannoprotein, was studied in yeast protoplast systems by using tunicamycin at various temperatures. At 30 degrees C, tunicamycin-treated protoplasts did not produce active...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mizunaga T,Izawa M,Ikeda K,Maruyama Y

    更新日期:1988-02-01 00:00:00

  • Stress-sensing mechanisms in the unfolded protein response: similarities and differences between yeast and mammals.

    abstract::The unfolded protein response is an adaptive stress response that responds to the imbalance between the entry of newly synthesized unfolded proteins and the inherent folding capacity in the endoplasmic reticulum (ER). Various environmental stresses and changes in physiological conditions can result in the accumulation...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章,评审


    authors: Kohno K

    更新日期:2010-01-01 00:00:00

  • Three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase. Optimization of the oxidative half-reaction through alignment of the product with reduced flavin.

    abstract::The three-dimensional structure of the purple intermediate of porcine kidney D-amino acid oxidase (DAO) was solved by cryo-X-ray crystallography; the purple intermediate is known to comprise a complex between the dehydrogenated product, an imino acid, and the reduced form of DAO. The crystalline purple intermediate wa...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Mizutani H,Miyahara I,Hirotsu K,Nishina Y,Shiga K,Setoyama C,Miura R

    更新日期:2000-07-01 00:00:00

  • Tissue-specific regulation of renin-binding protein gene expression in rats.

    abstract::Rat gene for renin-binding protein (RnBP) was shown to be expressed in the kidney, adrenal gland, brain, lung, spleen, ovary, testis, and heart. On sodium depletion and captopril administration, the rat showed a marked increase in the adrenal RnBP mRNA level and a slight decrease in the kidney RnBP mRNA level. In two-...

    journal_title:Journal of biochemistry

    pub_type: 杂志文章


    authors: Tada M,Takahashi S,Miyano M,Miyake Y

    更新日期:1992-08-01 00:00:00